Professor Peter Waterhouse
Publications and Presentations
Prof. Waterhouse has published one book (currently editing a second for Wiley-Blackwell), more than 30 book chapters or invited reviews and 101 papers in refereed international journals. Many of these papers and reviews have been in top echelon journals including: Nature, Science, Nature Genetics, Nature Biotechnology, Nature Reviews: Genetics, EMBO, EMBO Reports, Current Biology, PNAS, Trends in Plant Science, Blood and RNA. The Web of Science identifies 82 of these from the last 20 years and records 4626 citations, giving an average ~56 citations/publication and an h-index of 35. In 2003, he was presented with the IMTC-ISI/Thomson award for being the most highly cited CSIRO scientist for the five year period from 1998 to 2003. For each of the last five years, his papers have received between 300 and 400 citations per annum. Prof. Waterhouse has been regularly invited to give, mainly keynote and plenary talks at national and international conferences (>40 since 2000) and to organise and/or chair Symposia and International Conferences. A selection of his publications and talks are given below.
Top ten career-best publications
1. Fusaro AF, Matthew L, Smith NA, Curtin SJ, Dedic-Hagan J, Ellacott GA, Watson JM, Wang M-B, Brosnan C, Carroll BJ, and Waterhouse PM. (2006). RNAi-inducing hairpin RNAs in plants act through the viral defence pathway.
EMBO Reports 7 1168-1175
Citations: 41 (IF 7.2) This paper was the first to show that RNAi-inducing hpRNA and viral dsRNA are processed in plants by three different Dicer enzymes.
2. Wang M-B, Bian X-Y, Wu L-M, Liu L-X, Smith NA, Isenegger D, Wu RM, Masuta C, Vance VB, Watson JM, Rezaian A, Dennis ES and Waterhouse PM. (2004) On the role of RNA silencing in the pathogenicity and evolution of viroids and viral satellites.
Proc Natl Acad Sci USA 101, 3275-3280.
Citations: 63 (IF 9.6) This paper was the first to propose and show that pathogens caused symptoms through siRNAs and the RNAi pathway.
3. Waterhouse PM and Helliwell CA. (2003) Exploring plant genomes by RNA-induced gene silencing.
Nature Reviews: Genetics 4, 29-38.
Citations: 108 (IF 24.1) This invited review described and compared the various ways of delivering RNAi and discussed how they could be used for functional genomics research.
4. Finnegan EJ, Margis R and Waterhouse PM. (2003). Posttranscriptional Gene Silencing Is Not Compromised in the Arabidopsis Carpel Factory (Dicer-like1) Mutant, a Homolog of Dicer-1 from Drosophila.
Current Biology.13, 236-40.
Citations: 74 (IF 10.8) This paper showed that, unlike humans which posses a single Dicer, plants have multiple Dicers.
5. Waterhouse PM, Wang M-B and Lough T. (2001) Gene silencing as an adaptive defence against viruses.
Nature 411, 834-842.
Citations: 423 (IF 31.4) This invited review brought together many previously confusing elements to give a largely cohesive picture of the nature and function of gene silencing (now termed RNAi) in plants, and was the first to draw the parallel with the mammalian immune system.
6. Wesley SV, Helliwell, C, Smith NA, Wang M-B, Rouse D, Liu Q, Gooding P, Singh S, Abbott D, Stoutjesdijk P, Robinson S, Gleave A, Green A and Waterhouse PM. (2001) Constructs for Efficient, Effective and High Throughput Gene Silencing in Plants.
The Plant Journal 27, 581-590.
Citations: 438 (IF 6.4) Describes the application of intron-spliced hairpin RNA constructs to silence a wide range of genes in a wide range of species, reducing the theory to practice. It also describes the production of a recombinase vector for high throughput silencing such as needed for large scale genomic projects. Doubly cited in Faculty of 1000.
7. Smith NA, Singh SP,Wang M-B, Stoutjesdijk P, Green A and Waterhouse PM. (2000) Total silencing by intron-spliced hairpin RNAs.
Nature 407, 319-320.
Citations: 385 (IF 31.4) This was the first demonstration that intron-spliced hairpin RNAs direct very efficient gene silencing.
8. Waterhouse PM, Graham MW and Wang M-B. (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA.
Proc Natl Acad Sci USA 95, 13959-13964.
Citations: 486 (IF 9.6) This was the first demonstration that double stranded RNA and hairpin RNA caused gene silencing in plants. This independent study was published only months after the paper by Fire and Mello that showed that double stranded RNA causes gene silencing (RNA interference; RNAi) in nematodes and for which they were awarded the Nobel Prize in 2006.
9. Waterhouse P, Griffiths AD, Johnstone K & Winter G. (1993) Combinatorial infection and in vivo recombination: a strategy for making large phage antibody repertoires.
Nucleic Acids Research 21, 2265 – 2266
Citations: 89 (IF 6.8) Describes the invention of an in vivo recombination system which generates combinatorial heavy and light chain antibody libraries. It is key prior art in GatewayTM patents and facilitated the work described in the paper below (10).
10. Griffiths AD, Williams SC, Hartley O, Tomlinson IM, Waterhouse P, Crosby WL, Kontermann RE, Jones PT, Low NM, Allison TJ, Prospero TD, Hoogenboom, HR, Nissim A, Cox JPL, Harrison, JL, Zaccolo M, Gherardi E and Winter, G. (1994) Isolation of high affinity human antibodies directly from large synthetic repertoires.
The EMBO Journal 13, 3245 – 3260.
Citations: 624 (IF 8.9) Describes the production of a very large library of synthetic antibodies, using the strategy and vectors from (9), and the isolation of high affinity antibodies from it to a number of different antigens.
Five invited opinion pieces
1. Gordon KHJ and Waterhouse PM (2007). RNAi for insect-proof plants.
Nature Biotechnology 25, 1233-1234. (IF 22.2)
2. Waterhouse PM and Fusaro AF (2006). Viruses face a double defence by plant small RNAs. Science 313 :54-5. (IF 28.1)
3. Waterhouse PM (2006) Defense and counterdefense in the plant world.
Nature Genetics. 38:138-9 (IF 30.2)
4. Hetherington, A and Waterhouse PM (2002). The complexity of signals and levels of gene regulation in plants.
Current Opinion in Plant Biology 5, 373-¬375 (IF 8.7)
5. Finnegan, E.J., Wang, M-B. and Waterhouse PM (2001) Gene Silencing: Fleshing out the bones. Current Biology 11, 99-102. (IF 10.8)
Five recent significant publications
1. Eamens AL, Smith NA, Curtin SJ, Wang M-B, and Waterhouse PM (2009) The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes.
RNA epub doi:10.1261/rna.1646909 (IF 5.0)
2. Pase L, Layton JE, Kloosterman WP, Carradice D, Waterhouse PM, Lieschke GJ. (2009) miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2.
Blood. 113 :1794-804. (Cit 4, IF 10.4)
3. Curtin SJ, Watson JM, Smith NA, Eamens AL, Blanchard CL, Waterhouse PM. (2008) The roles of plant dsRNA-binding proteins in RNAi-like pathways.
FEBS Letters. 582 2753-60. (Cit 2, IF 3.3)
4. Eamens A, Wang MB, Smith NA, Waterhouse PM. . (2008) RNA silencing in plants: yesterday, today, and tomorrow
Plant Physiology 147, 456-68 (Cit 10, IF 6.7)
5. Brosnan CA, Mitter N, Christie M, Smith NA, Waterhouse PM and Carroll BJ. (2007) Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis.
Proc Natl Acad Sci USA 104, 14741-14746 (Cit 32, IF 9.6)
• Five recent plenary talks:
Epigenetics-2007, Perth, Australia 2007
International Plant Physiology Meeting, Rio de Janeiro, Brazil, 2007
4th Int. Rice Functional Genomics Meeting, Montpellier, France, 2006
Regulatory RNA Symposium, Taipei, Taiwan, 2006
Functional Genomics of Maize Symposium, Irapuato, Mexico, 2005
• Five recent keynote talks:
56th Annual conference: Genetics Society of Australasia, Brisbane, 2009
Plant and Animal Genome Conference, San Diego, USA, 2008,
Invitrogen : Current Topics Symposium 2008, San Diego, USA, 2008
BioAsia 2007, Bangkok, Thailand 2007
COMBIO 2007, Sydney, 2007
• Five talks at “by-invitation-only” conferences
US, UK & Aust. Epigenome Conference, Blue Mountains, Australia, 2008
Pattern Formation and Functional Morphology, Linz, Austria, 2008
Banbury Center :RNAi-Related Processes in Plants: Chromatin, Development and Defense, Cold Spring Harbor, USA, 2004.
Banbury Center: RNA mediated Silencing, CSH, USA, 2000
FMI & Novartis: Epigenetic Gene Silencing, Brunnen, Switzerland, 2000
Current national competitive grants*
Extreme expression: building a platform for industrial plant biotechnology
Dale J, Waterhouse P, Harding R, Coleman H, Dugdale B, Jackson C
Queensland University of Technology Shared Research Support ($278,400 over 3 years)
* Grants administered through the University of Sydney
New Zealand. (Plant and Food Research) Part of a Genome sequencing Consortium.