Associate Professor Charles Collyer

Associate Dean

G08 - Biochemistry and Microbiology Building
The University of Sydney

Telephone +61 2 9351 2794
Fax +61 2 9351 4726

Research interests

Charles' research focuses on the fundamental relationship between the atomic structure of molecules and how they function in cells. The method of Protein Crystallography has made a major contribution to our understanding of enzyme mechanism, molecular recognition, and to our growing knowledge of the mechanics of molecular machines. Research projects in structural biology are aimed to contribute to areas such as drug design and protein engineering. A key focus of his recent efforts has been in the application of protein crystallography to biomedical research.

Selected grants

2013

  • Multiangle laser light scattering instrument for protein characterisation; Mackay J, Ataide S, Jormakka M, Ryan R, Matthews J, Shepherd N, Sunde M, Collyer C, Payne R; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2006

  • Structure-based inhibitor design of VAP-1/SSAO for the treatment of respiratory dirsorders and other major inflammatory diseases; Guss M, McDonald I, Collyer C, McDonald I; Australian Research Council (ARC)/Linkage Projects (LP).

2005

  • Synthesis and evaluation of anti-microbial porphyrin adducts for the targeted inhibition of Porphyromonas gingivalis; Crossley M, Collyer C, Hunter N, Hunter N; Australian Research Council (ARC)/Linkage Projects (LP).

2000

  • Preliminary research in the development of prophylaxis and treatment of gingivitis periodontal disease agreement between the Biochemical Veterinary Research Pty Ltd and The University of Sydney; Lucas P, Biffin J, Craft J, Collyer C, Crossley M; Biochemical Veterinary Research (BVR) Pty Ltd/BLO Project.

Selected publications

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Book Chapters

  • Goerigk, L., Falklof, O., Collyer, C., Reimers, J. (2012). First Steps Towards Quantum Refinement of Protein X-ray Structures. In Jun Zeng, Rui-Qin Zhang, Herbert R. Treutlein (Eds.), Quantum Simulations of Materials and Biological Systems, (pp. 87-120). London: Springer.
  • Kekic, M., Nosworthy, N., Dedova, I., Collyer, C., Dos Remedios, C. (2001). Regulation of the cytoskeleton assembly: a role for a ternary complex of actin with two actin-binding proteins. In Cristobal G. Dos Remedios & David D. Thomas (Eds.), Molecular Interactions of Actin: Actin Structure and Actin-Binding Proteins, (pp. 165-177). Germany: Springer.

Journals

  • Yun, P., Li, N., Collyer, C., Hunter, N. (2012). The K1K2 Region of Lys-Gingipain of Porphyromonas gingivalis Blocks Induction of HLA Expression by Gamma Interferon. Infection and Immunity, 80(10), 3733-3741. [More Information]
  • Falklof, O., Collyer, C., Reimers, J. (2012). Toward ab initio refinement of protein X-ray crystal structures: interpreting and correlating structural fluctuations. Theoretical Chemistry Accounts: theory, computation and modeling, 131(1), 1-16. [More Information]
  • McGrath, A., Mithieux, S., Collyer, C., Bakhuis, J., van den Berg, M., Sein, A., Heinz, A., Schmelzer, C., Weiss, A., Guss, M. (2011). Structure and Activity of Aspergillus nidulans Copper Amine Oxidase. Biochemistry, 50(25), 5718-5730. [More Information]
  • Li, N., Yun, P., Jeffries, C., Langley, D., Gamsjaeger, R., Church, W., Hunter, N., Collyer, C. (2011). The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Molecular Microbiology, 81(5), 1358-1373. [More Information]
  • McGrath, A., Hilmer, K., Collyer, C., Dooley, D., Guss, M. (2010). A new crystal form of human diamine oxidase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(2), 137-142. [More Information]
  • Ernberg, K., McGrath, A., Peat, T., Adams, T., Xiao, X., Pham, T., Newman, J., McDonald, I., Collyer, C., Guss, M. (2010). A new crystal form of human vascular adhesion protein 1. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(Pt 12), 1572-1578. [More Information]
  • McGrath, A., Caradoc-Davies, T., Collyer, C., Guss, M. (2010). Correlation of Active Site Metal Content in Human Diamine Oxidase with Trihydroxyphenylalanine Quinone Cofactor Biogenesis. Biochemistry, 49(38), 8316-8324. [More Information]
  • Li, N., Yun, P., Nadkarni, M., Nazila, B., Nguyen, K., Lee, M., Hunter, N., Collyer, C. (2010). Structure determination and analysis of a haemolytic gingipain adhesin domain from Porphyromonas gingivalis. Molecular Microbiology, 76(4), 861-873. [More Information]
  • Yap, B., Simpkins, G., Collyer, C., Hunter, N., Crossley, M. (2009). Porphyrin-linked nitroimidazole antibiotics targeting: Prophyromonas gingivalis. Organic and Biomolecular Chemistry, 7(14), 2855-2863. [More Information]
  • McGrath, A., Hilmer, K., Collyer, C., Shephard, E., Elmore, B., Brown, D., Dooley, D., Guss, M. (2009). Structure and inhibition of human diamine oxidase. Biochemistry, 48(41), 9810-9822. [More Information]
  • Park, J., Knott, H., Nadvi, N., Collyer, C., Wang, X., Church, W., Gorrell, M. (2008). Reversible Inactivation of Human Dipeptidyl Peptidases 8 and 9 by Oxidation. The Open Enzyme Inhibition Journal, 1, 52-60.
  • Langley, D., Harty, D., Jacques, N., Hunter, N., Guss, M., Collyer, C. (2008). Structure of N-acetyl-B-D-glucosaminidase (GcnA) from the Endocarditis Pathogen Streptococcus gordonii and its Complex with the Mechanism-based Inhibitor NAG-thiazoline. Journal of Molecular Biology, 377, 104-116. [More Information]
  • Jeffries, C., Graham, S., Stokes, P., Collyer, C., Guss, M., Matthews, J. (2006). Stabilization of a binary protein complex by intein-mediated cyclization. Protein Science, 15(11), 2612-2618. [More Information]
  • Mun, H., Culverston, E., Franks, A., Collyer, C., Clifton-Bligh, R., Conigrave, A. (2005). A double mutation in the extracellular Ca2+-sensing receptor's venus flytrap domain that selectively disables L-amino acid sensing. Journal of Biological Chemistry, 280(32), 29067-29072. [More Information]
  • Yun, P., DeCarlo, A., Chapple, C., Collyer, C., Hunter, N. (2005). Binding of Porphyromonas gingivalis gingipains to human CD4(+) T cells preferentially down-regulates surface CD2 and CD4 with little affect on co-stimulatory molecule expression. Microbial Pathogenesis, 38(2-3), 85-96. [More Information]
  • Collyer, C., Templeton, M., Reinhardt, L., Mitchell, R., Cleland, W. (2005). Kinetic analysis of the L-ornithine transcarbamoylase from Pseudomonas savastanoi pv. phaseolicola that is resistant to the transition state analogue (R)-N delta-(N'-sulfodiaminophosphinyl)-L-ornithine. Biochemistry, 44(11), 4408-15. [More Information]
  • Hunter, N., Yap, B., Collyer, C., Crossley, M. (2005). Targeting of a key pathogen in a polymicrobial infection. Microbiology Australia, 26(3), 122-123.
  • Langley, D., Harty, D., Graham, S., Guss, M., Hunter, N., Collyer, C. (2004). Crystallization Of Gcna, An N-Acetyl-β-D-Glucosaminidase, From Streptococcus Gordonii. Acta Crystallographica. Section D: Biological Crystallography, D60, 1910-1911.
  • Nguyen, K., DeCarlo, A., Paramaesvaran, M., Collyer, C., Langley, D., Hunter, N. (2004). Humoral Responses To Porphyromonas gingivalis Gingipain Adhesin Domains In Subjects With Chronic Periodontitis. Infection and Immunity, 72(3), 1374-1382.
  • DeCarlo, A., Nadkarni, M., Paramaesvaran, M., Yun, P., Collyer, C., Hunter, N. (2004). Serum Antibodies Against The Hemoglobin-Binding Domain (HA2) Of Porphyromonas Gingivalis. Journal of Periodontal Research, 39(4), 228-235.
  • Maher, M., Huang, D., Guss, M., Collyer, C., Christopherson, R. (2003). Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form. Acta Crystallographica. Section D: Biological Crystallography, 59(2), 381-384.
  • Yun, L., DeCarlo, A., Collyer, C., Hunter, N. (2003). Enhancement of Th2 pathways and direct activation of B cells by the gingipains of Porphyromonas gingivalis. Clinical and Experimental Immunology, 134(2), 295-302.
  • DeCarlo, A., Huang, Y., Collyer, C., Langley, D., Katz, J. (2003). Feasibility of an HA2 domain-based periodontitis vaccine. Infection and Immunity, 71(1), 562-566.
  • Paramaesvaran, M., Nguyen, K., Caldon, E., McDonald, J., Najdi, S., Gonzaga, G., Langley, D., DeCarlo, A., Hunter, N., Crossley, M., Collyer, C. (2003). Porphyrin-mediated cell surface heme capture from hemoglobin by Porphyromanas gingivalis. Journal of Bacteriology, 185(8), 2528-2537.
  • Yun, P., DeCarlo, A., Collyer, C., Hunter, N. (2002). Modulation of an interleukin-12 and interferon gamma synergistic feedback regulatory cycle of T-cell/monocyte co-cultures by Porphyromonas gingivalis lipopolysaccharide in the absence or presence of cysteine proteinases. Infection and Immunity, 70(10), 5695-5705.
  • Hunter, N., Nguyen, K., McDonald, J., Quinn, M., Langley, D., Crossley, M., Collyer, C. (2002). Structural requirements for recognition of essential porphyrin by Porphyromonas gingivalis. Journal of Porphyrins and Phthalocyanines, 6(12), 774-782.
  • Collyer, C., Yun,, P., Decarlo, A., Hunter,, N. (2001). Hydrolysis of interleukin-12 by Porphyromonas gingivalis major cysteine proteinases may affect local gamma interferon accumulation and the Th1 or Th2 T-Cell phenotype in periodontitis. Infection and Immunity, 69, No. 9, 5650-5660.

Patents

  • Hunter, N., Collyer, C., De Carlo, A. (2010). Prophylaxis And Treatment Of P. Gingivalis Infection. Patent No. 1189630, 60043936.4-08. Germany.
  • Hunter, N., Collyer, C., DeCarlo, A. (2008). A Method Of Prophylaxis And Treatment And Agents Useful For Same. Patent No. 515853, 7319086, 781704. New Zealand.

2012

  • Goerigk, L., Falklof, O., Collyer, C., Reimers, J. (2012). First Steps Towards Quantum Refinement of Protein X-ray Structures. In Jun Zeng, Rui-Qin Zhang, Herbert R. Treutlein (Eds.), Quantum Simulations of Materials and Biological Systems, (pp. 87-120). London: Springer.
  • Yun, P., Li, N., Collyer, C., Hunter, N. (2012). The K1K2 Region of Lys-Gingipain of Porphyromonas gingivalis Blocks Induction of HLA Expression by Gamma Interferon. Infection and Immunity, 80(10), 3733-3741. [More Information]
  • Falklof, O., Collyer, C., Reimers, J. (2012). Toward ab initio refinement of protein X-ray crystal structures: interpreting and correlating structural fluctuations. Theoretical Chemistry Accounts: theory, computation and modeling, 131(1), 1-16. [More Information]

2011

  • McGrath, A., Mithieux, S., Collyer, C., Bakhuis, J., van den Berg, M., Sein, A., Heinz, A., Schmelzer, C., Weiss, A., Guss, M. (2011). Structure and Activity of Aspergillus nidulans Copper Amine Oxidase. Biochemistry, 50(25), 5718-5730. [More Information]
  • Li, N., Yun, P., Jeffries, C., Langley, D., Gamsjaeger, R., Church, W., Hunter, N., Collyer, C. (2011). The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Molecular Microbiology, 81(5), 1358-1373. [More Information]

2010

  • McGrath, A., Hilmer, K., Collyer, C., Dooley, D., Guss, M. (2010). A new crystal form of human diamine oxidase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(2), 137-142. [More Information]
  • Ernberg, K., McGrath, A., Peat, T., Adams, T., Xiao, X., Pham, T., Newman, J., McDonald, I., Collyer, C., Guss, M. (2010). A new crystal form of human vascular adhesion protein 1. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(Pt 12), 1572-1578. [More Information]
  • McGrath, A., Caradoc-Davies, T., Collyer, C., Guss, M. (2010). Correlation of Active Site Metal Content in Human Diamine Oxidase with Trihydroxyphenylalanine Quinone Cofactor Biogenesis. Biochemistry, 49(38), 8316-8324. [More Information]
  • Hunter, N., Collyer, C., De Carlo, A. (2010). Prophylaxis And Treatment Of P. Gingivalis Infection. Patent No. 1189630, 60043936.4-08. Germany.
  • Li, N., Yun, P., Nadkarni, M., Nazila, B., Nguyen, K., Lee, M., Hunter, N., Collyer, C. (2010). Structure determination and analysis of a haemolytic gingipain adhesin domain from Porphyromonas gingivalis. Molecular Microbiology, 76(4), 861-873. [More Information]

2009

  • Yap, B., Simpkins, G., Collyer, C., Hunter, N., Crossley, M. (2009). Porphyrin-linked nitroimidazole antibiotics targeting: Prophyromonas gingivalis. Organic and Biomolecular Chemistry, 7(14), 2855-2863. [More Information]
  • McGrath, A., Hilmer, K., Collyer, C., Shephard, E., Elmore, B., Brown, D., Dooley, D., Guss, M. (2009). Structure and inhibition of human diamine oxidase. Biochemistry, 48(41), 9810-9822. [More Information]

2008

  • Hunter, N., Collyer, C., DeCarlo, A. (2008). A Method Of Prophylaxis And Treatment And Agents Useful For Same. Patent No. 515853, 7319086, 781704. New Zealand.
  • Park, J., Knott, H., Nadvi, N., Collyer, C., Wang, X., Church, W., Gorrell, M. (2008). Reversible Inactivation of Human Dipeptidyl Peptidases 8 and 9 by Oxidation. The Open Enzyme Inhibition Journal, 1, 52-60.
  • Langley, D., Harty, D., Jacques, N., Hunter, N., Guss, M., Collyer, C. (2008). Structure of N-acetyl-B-D-glucosaminidase (GcnA) from the Endocarditis Pathogen Streptococcus gordonii and its Complex with the Mechanism-based Inhibitor NAG-thiazoline. Journal of Molecular Biology, 377, 104-116. [More Information]

2006

  • Jeffries, C., Graham, S., Stokes, P., Collyer, C., Guss, M., Matthews, J. (2006). Stabilization of a binary protein complex by intein-mediated cyclization. Protein Science, 15(11), 2612-2618. [More Information]

2005

  • Mun, H., Culverston, E., Franks, A., Collyer, C., Clifton-Bligh, R., Conigrave, A. (2005). A double mutation in the extracellular Ca2+-sensing receptor's venus flytrap domain that selectively disables L-amino acid sensing. Journal of Biological Chemistry, 280(32), 29067-29072. [More Information]
  • Yun, P., DeCarlo, A., Chapple, C., Collyer, C., Hunter, N. (2005). Binding of Porphyromonas gingivalis gingipains to human CD4(+) T cells preferentially down-regulates surface CD2 and CD4 with little affect on co-stimulatory molecule expression. Microbial Pathogenesis, 38(2-3), 85-96. [More Information]
  • Collyer, C., Templeton, M., Reinhardt, L., Mitchell, R., Cleland, W. (2005). Kinetic analysis of the L-ornithine transcarbamoylase from Pseudomonas savastanoi pv. phaseolicola that is resistant to the transition state analogue (R)-N delta-(N'-sulfodiaminophosphinyl)-L-ornithine. Biochemistry, 44(11), 4408-15. [More Information]
  • Hunter, N., Yap, B., Collyer, C., Crossley, M. (2005). Targeting of a key pathogen in a polymicrobial infection. Microbiology Australia, 26(3), 122-123.

2004

  • Langley, D., Harty, D., Graham, S., Guss, M., Hunter, N., Collyer, C. (2004). Crystallization Of Gcna, An N-Acetyl-β-D-Glucosaminidase, From Streptococcus Gordonii. Acta Crystallographica. Section D: Biological Crystallography, D60, 1910-1911.
  • Nguyen, K., DeCarlo, A., Paramaesvaran, M., Collyer, C., Langley, D., Hunter, N. (2004). Humoral Responses To Porphyromonas gingivalis Gingipain Adhesin Domains In Subjects With Chronic Periodontitis. Infection and Immunity, 72(3), 1374-1382.
  • DeCarlo, A., Nadkarni, M., Paramaesvaran, M., Yun, P., Collyer, C., Hunter, N. (2004). Serum Antibodies Against The Hemoglobin-Binding Domain (HA2) Of Porphyromonas Gingivalis. Journal of Periodontal Research, 39(4), 228-235.

2003

  • Maher, M., Huang, D., Guss, M., Collyer, C., Christopherson, R. (2003). Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form. Acta Crystallographica. Section D: Biological Crystallography, 59(2), 381-384.
  • Yun, L., DeCarlo, A., Collyer, C., Hunter, N. (2003). Enhancement of Th2 pathways and direct activation of B cells by the gingipains of Porphyromonas gingivalis. Clinical and Experimental Immunology, 134(2), 295-302.
  • DeCarlo, A., Huang, Y., Collyer, C., Langley, D., Katz, J. (2003). Feasibility of an HA2 domain-based periodontitis vaccine. Infection and Immunity, 71(1), 562-566.
  • Paramaesvaran, M., Nguyen, K., Caldon, E., McDonald, J., Najdi, S., Gonzaga, G., Langley, D., DeCarlo, A., Hunter, N., Crossley, M., Collyer, C. (2003). Porphyrin-mediated cell surface heme capture from hemoglobin by Porphyromanas gingivalis. Journal of Bacteriology, 185(8), 2528-2537.

2002

  • Yun, P., DeCarlo, A., Collyer, C., Hunter, N. (2002). Modulation of an interleukin-12 and interferon gamma synergistic feedback regulatory cycle of T-cell/monocyte co-cultures by Porphyromonas gingivalis lipopolysaccharide in the absence or presence of cysteine proteinases. Infection and Immunity, 70(10), 5695-5705.
  • Hunter, N., Nguyen, K., McDonald, J., Quinn, M., Langley, D., Crossley, M., Collyer, C. (2002). Structural requirements for recognition of essential porphyrin by Porphyromonas gingivalis. Journal of Porphyrins and Phthalocyanines, 6(12), 774-782.

2001

  • Collyer, C., Yun,, P., Decarlo, A., Hunter,, N. (2001). Hydrolysis of interleukin-12 by Porphyromonas gingivalis major cysteine proteinases may affect local gamma interferon accumulation and the Th1 or Th2 T-Cell phenotype in periodontitis. Infection and Immunity, 69, No. 9, 5650-5660.
  • Kekic, M., Nosworthy, N., Dedova, I., Collyer, C., Dos Remedios, C. (2001). Regulation of the cytoskeleton assembly: a role for a ternary complex of actin with two actin-binding proteins. In Cristobal G. Dos Remedios & David D. Thomas (Eds.), Molecular Interactions of Actin: Actin Structure and Actin-Binding Proteins, (pp. 165-177). Germany: Springer.

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