Professor David James

FAA
Leonard P. Ullmann Chair of Metabolic Systems Biology, Charles Perkins Centre
Professor, School of Life & Environmental Sciences and Sydney Medical School

D17 - Charles Perkins Centre
The University of Sydney

Telephone +61 2 8627 1621

Website Metabolic Cybernetics

Biographical details

David James was awarded a PhD in 1985 in Physiology and Biochemistry from UNSW. During this period, together with colleagues at the Garvan Institute, he developed novel methodologies for studying in vivo glucose metabolism in small animals leading to a number of important discoveries about the role of tissue specificity of insulin action at the whole body level.

In 1985 he was awarded a Fogarty fellowship and later a Juvenile Diabetes Foundation fellowship to undertake postdoctoral training at Boston University with Paul Pilch and subsequently at Washington University in St Louis. During this period he provided cellular and molecular evidence to document the identity and behaviour of the insulin responsive glucose transporter GLUT4.

In 1989 he established his own independent career as Assist/Professor at Washington University in St Louis funded by a Career Development Award from the Juvenile Diabetes Foundation. During this period he mapped many of the molecules and pathways that are known to be involved in the cell biology of insulin regulated glucose transport in muscle and fat cells. In 1993 he returned to Australia on a Wellcome Trust Senior Research Fellowship taking up a position at the IMB in Brisbane. During this period his laboratory discovered a number of key proteins and mechanisms that regulate the docking and fusion of vesicles with the plasma membrane.

In 2002 he moved to Sydney to head up the Diabetes and Obesity Research Program at the Garvan Institute as an NHMRC Senior Principal Research Fellow where he remained until February 2014. He currently holds the Leonard P Ullmann Chair in Molecular Systems Biology and he is the Domain Leader for Biology at the Charles Perkins Centre, Sydney University.

Since returning to Australia he has won several awards including the Glaxo Wellcome Medal for Medical Research and the Kellion medal for outstanding contributions to Diabetes research. In 2007 he was elected as a fellow of the Australian Academy of Science.

He is currently on the editorial boards of Cell Metabolism, Molecular Cell Biology, Traffic and Journal Clinical Investigation. He was the founding member of the Asia Pacific Diabetes and Obesity study group with Professor Masato Kasuga and he is currently the President of the Hunter Meeting Inc.

He has supervised 23 PhD students and 25 postdoctoral fellows, most of whom remain actively engaged in research throughout the world.

James has published >200 original research papers and >25 invited reviews in his research area many of which have been cited >500 times.

Current research students

Project title Research student
Unravelling the regulation of phosphorylation from temporal large-scale phosphoproteomics studies Westa DOMANOVA
Lipid regulation if inflammatory pain John MANION
Investigation and Quantification of FES Exercise - Isometric Electromechanics and Perceptions of Its Usage as an Exercise Modality for Various Populations Matthew TAYLOR

Selected grants

2017

  • A multiple ion beam facility for Microscopy and Nanofabrication; Palomba S, Ringer S, Cairney J, Shadrivov I, Toth M, Simpson S, Phillips M, Bagnall D, Bradby J, Chen V, James D, Wallace G; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • Probing the Gene-Environment Interaction in Metabolic Disease; James D; National Health and Medical Research Council (NHMRC)/Career Awards: Research Fellowships.

2016

  • Advanced Microfluidics for Live Cell Imaging; James D, Palomba S, Neely G; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Protein Technologies Prelude� X Automated Parallel Peptide Synthesiser; Payne R, James D, Jackson S; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Ultra-high pressure system for proteomic analysis of a multi-ethnic cohort to identify biomarkers for type-2 diabetes; James D, Parker B, Humphrey S; Ian Potter Foundation/Medical Research.
  • Molecular pathways linking nutrition and age-related health; Simpson S, Le Couteur D, Cogger V, James D; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Is FGF21 the master regulator of protein intake?; Simpson S, James D, Raubenheimer D, Conigrave A, Le Couteur D; Australian Research Council (ARC)/Discovery Projects (DP).
  • A systems biology capability for the Ramaciotti Centre for Genomics; Cordwell S, James D, Simpson S, Payne R, Wilkins M, Cavicchioli R, Packer N, Guillemin G, Goodchild A, Scott R, Thorne R, Hondermarck H, Djordjevic S, McDougald D, Myers G; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • A multi-ethnic cohort and intervention trial to identify early biomarkers for type 2 diabetes and customise individualized environments for disease prevention; James D, Hocking S, Zeng R, Wu J, Brand-Miller J, Bauman A, Yang J, Sun L, Li R, Li Q; National Health and Medical Research Council (NHMRC)/National Natural Science Foundation of China (NSFC).

2015

  • USYD Research Support in Metabolic Systems Biology - New Initiatives; James D; University of Sydney/Internal Scheme.
  • Mitochondria as a key node of metabolic disease; James D; Rebecca L Cooper Medical Research Foundation/Equipment Grant.
  • Probing the exercise biochemistry landscape for new therapeutic options for treating diabetes; James D, Chaudhuri R, Hoffman N; Diabetes Australia Research Trust (DART)/Research Grants.
  • Defining factors that contribute to individual diversity in the diet-health axis; James D, Neely G, Neely G, Simpson S; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Spinning Disk Confocal System for Ultra-High Speed Imaging; James D, Braet F, Jackson S, Bilek M, Overall R; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Metabolic wiring in adipocytes - unique role in maintaining long-term health; James D, Cooney G, Cooney G; National Health and Medical Research Council (NHMRC)/Project Grants.
  • AKTA Pure 25 M2 with dedicated chromatography columns & Laboratory Refrigerator; Mackay J, Ataide S, Sunde M, Balle T, James D; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2014

  • Towards real time data sharing to support patient-centred care, quality health delivery, improvement programmes, performance management and research; Morris J, Roberts C, Simpson S, Kay J, Venkatesh S, Fazekas de St Groth B, Arthur J, Feng D, Holmes E, James D, Yang J, Engel A; NSW Ministry of Health, Office for Health and Medical Research/Bioinformatics Collaborative Grants Program.
  • Research Fellowship - SPRF; James D; National Health and Medical Research Council (NHMRC)/Career Awards: Research Fellowships.
  • Understanding the link between obesity, impaired insulin action and long term health; James D; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Mechanistic studies of insulin action; Stoeckli J, James D; National Health and Medical Research Council (NHMRC)/Project Grants.

2013

  • Defining how insulin regulates glucose uptake into muscle and fat, one of the major steps that is defective in Type 2 diabetes; James D, Stoeckli J, Lambright D, Hughes W; National Health and Medical Research Council (NHMRC)/Project Grants.

2008

  • Defining the insulin-signalling defect in human insulin resistance and type 2 diabetes; James D, Greenfield J; Diabetes Australia Research Trust (DART)/Research Grants.
  • Rebecca Cooper Foundation Grant; James D; Rebecca Cooper Medical Research Foundation/Rebecca Cooper Medical Research Foundation.

Selected publications

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Book Chapters

  • Govers, R., James, D., Coster, A. (2008). High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins. In Andrei I. Ivanov (Eds.), Exocytosis and Endocytosis, (pp. 129-146). New Jersey, United States of America: Humana Press.

Journals

  • Schoenwaelder, S., Darbousset, R., Cranmer, S., Ramshaw, H., Orive, S., Sturgeon, S., Yuan, Y., Yao, Y., Krycer, J., Woodcock, J., Maclean, J., James, D., Jackson, S., et al (2016). 14-3-3z regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function. Nature Communications, 7, 1-15. [More Information]
  • Wu, L., Levina, A., Harris, H., Cai, Z., Lai, B., Vogt, S., James, D., Lay, P. (2016). Carcinogenic chromium(VI) compounds formed by intracellular oxidation of chromium(III) dietary supplements by adipocytes. Angewandte Chemie: International Edition, 55(5), 1742-1745. [More Information]
  • Solon-Biet, S., Cogger, V., Pulpitel, T., Heblinski, M., Wahl, D., McMahon, A., Warren, A., Durrant-Whyte, J., Walters, K., Krycer, J., Ponton, F., Gokarn, R., Wali, J., Conigrave, A., James, D., Raubenheimer, D., Le Couteur, D., Simpson, S., et al (2016). Defining the Nutritional and Metabolic Context of FGF21 Using the Geometric Framework. Cell Metabolism, 24(4), 555-565. [More Information]
  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Stockli, J., James, D. (2016). Hyper-activation of the insulin signaling pathway improves intracellular proteostasis by coordinately upregulating the proteostatic machinery in adipocytes. Journal of Biological Chemistry, in press. [More Information]
  • Davey, J., Watt, K., Parker, B., Chaudhuri, R., Ryall, J., Cunningham, L., Qian, H., Sartorelli, V., Sandri, M., Chamberlain, J., James, D., et al (2016). Integrated expression analysis of muscle hypertrophy identifies Asb2 as a negative regulator of muscle mass. JCI Insight, 1(5), e85477. [More Information]
  • Yang, P., Patrick, E., Humphrey, S., Ghazanfar, S., James, D., Jothi, R., Yang, J. (2016). KinasePA: Phosphoproteomics data annotation using hypothesis driven kinase perturbation analysis. Proteomics, 16(13), 1868-1871. [More Information]
  • Brandon, A., Stuart, E., Leslie, S., Hoehn, K., James, D., Kraegen, E., Turner, N., Cooney, G. (2016). Minimal impact of age and housing temperature on the metabolic phenotype of Acc2K/K mice. Journal of Endocrinology, 228(3), 127-134. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stockli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17, 29-36. [More Information]
  • Kleinert, M., Parker, B., Chaudhuri, R., Fazakerley, D., Serup, A., Thomas, K., Krycer, J., Sylow Hansen, L., Fritzen, A., Hoffman, N., James, D., et al (2016). mTORC2 and AMPK differentially regulate muscle triglyceride content via Perilipin 3. Molecular Metabolism, 5(8), 646-655. [More Information]
  • Vafaee, F., Krycer, J., Ma, X., Burykin, T., James, D., Kuncic, Z. (2016). ORTI: An Open-Access Repository of Transcriptional Interactions for Interrogating Mammalian Gene Expression Data. PloS One, 11(10), 1-21. [More Information]
  • Yang, P., Humphrey, S., James, D., Yang, J., Jothi, R. (2016). Positive-unlabeled ensemble learning for kinase substrate prediction from dynamic phosphoproteomics data. Bioinformatics, 32(2), 252-259. [More Information]
  • Rogers, S., Fey, D., McCloy, R., Parker, B., Mitchell, N., Payne, R., Daly, R., James, D., Caldon, C., Watkins, D., et al (2016). PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells. Journal of Cell Science, 129(7), 1340-1354. [More Information]
  • Tonks, K., Coster, A., Christopher, M., Chaudhuri, R., Xu, A., Gagnon-Bartsch, J., Chisholm, D., James, D., Meikle, P., Greenfield, J., et al (2016). Skeletal Muscle and Plasma Lipidomic Signatures of Insulin Resistance and Overweight/Obesity in Humans. Obesity, 24(4), 908-916. [More Information]
  • Parker, B., Thaysen-Andersen, M., Fazakerley, D., Holliday, M., Packer, N., James, D. (2016). Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes. Molecular and Cellular Proteomics, 15(1), 141-153. [More Information]
  • Domanova, W., Krycer, J., Chaudhuri, R., Yang, P., Vafaee, F., Fazakerley, D., Humphrey, S., James, D., Kuncic, Z. (2016). Unraveling Kinase Activation Dynamics Using Kinase-Substrate Relationships from Temporal Large-Scale Phosphoproteomics Studies. PloS One, 11(6), 1-14. [More Information]
  • Wong, M., Krycer, J., Burchfield, J., James, D., Kuncic, Z. (2015). A generalised enzyme kinetic model for predicting the behaviour of complex biochemical systems. FEBS Open Bio, 5, 226-239. [More Information]
  • Yang, G., Murashige, D., Humphrey, S., James, D. (2015). A Positive Feedback Loop between Akt and mTORC2 via SIN1 Phosphorylation. Cell Reports, 12(6), 937-943. [More Information]
  • Lee, J., Walsh, M., Hoehn, K., James, D., Wherry, E., Choi, Y. (2015). Acetyl CoA Carboxylase 2 Is Dispensable for CD8+ T Cell Responses. PloS One, 10(9), 1-9. [More Information]
  • Kee, A., Yang, L., Lucas, C., Greenberg, M., Martel, N., Leong, G., Hughes, W., Cooney, G., James, D., Ostap, M., et al (2015). An Actin Filament Population Defined by the Tropomyosin Tpm3.1 Regulates Glucose Uptake. Traffic, 16(7), 691-711. [More Information]
  • Ma, X., Lee, P., Chisholm, D., James, D. (2015). Control of adipocyte differentiation in different fat depots; Implications for pathophysiology or therapy. Frontiers in Endocrinology, 6, 1-8. [More Information]
  • Chaudhuri, R., Khoo, P., Tonks, K., Junutula, J., Kolumam, G., Modrusan, Z., Samocha-Bonet, D., Meoli, C., Hocking, S., Fazakerley, D., Stockli, J., Yang, J., James, D., et al (2015). Cross-species gene expression analysis identifies a novel set of genes implicated in human insulin sensitivity. Npj Systems Biology And Applications, 1, 1-10. [More Information]
  • Rogers, S., McCloy, R., Parker, B., Chaudhuri, R., Gayevskiy, V., Hoffman, N., Watkins, D., Daly, R., James, D., Burgess, A. (2015). Dataset from the global phosphoproteomic mapping of early mitotic exit in human cells. Data in Brief, 5, 45-52. [More Information]
  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • McCloy, R., Parker, B., Rogers, S., Chaudhuri, R., Gayevskiy, V., Hoffman, N., Ali, N., Watkins, N., Daly, R., James, D., et al (2015). Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs. Molecular and Cellular Proteomics, 14(8), 2194-2212. [More Information]
  • Trefely, S., Khoo, P., Krycer, J., Chaudhuri, R., Fazakerley, D., Parker, B., Sultani, G., Lee, J., Stephan, J., Torres, E., James, D., Stoeckli, J., et al (2015). Kinome Screen Identifies PFKFB3 and Glucose Metabolism as Important Regulators of the Insulin/IGF-1 Signalling Pathway. The Journal of Biological Chemistry, 290(43), 25834-25846. [More Information]
  • Phua, Y., Nguyen, A., Roden, D., Elsworth, B., Deng, N., Nikolic, I., Yang, J., McFarland, A., Russell, R., Kaplan, W., O'Toole, S., James, D., et al (2015). MicroRNA profiling of the pubertal mouse mammary gland identifies miR-184 as a candidate breast tumour suppressor gene. Breast Cancer Research, 17(1), 1-18. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Humphrey, S., James, D., Mann, M. (2015). Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation. Trends in Endocrinology and Metabolism, 26(12), 676-687. [More Information]
  • Fazakerley, D., Naghiloo, S., Chaudhuri, R., Koumanov, F., Burchfield, J., Thomas, K., Krycer, J., Prior, M., Parker, B., Murrow, B., Stoeckli, J., Meoli, C., James, D., et al (2015). Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumour Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin Action in Adipocytes. The Journal of Biological Chemistry, 290(39), 23528-23542. [More Information]
  • Tan, S., Fisher_Wellman, K., Fazakerley, D., Ng, Y., Pant, H., Li, J., Meoli, C., Coster, A., Stoeckli, J., James, D. (2015). Selective insulin resistance in adipocytes. The Journal of Biological Chemistry, 290(18), 11337-11348. [More Information]
  • Ma, D., Stolte, C., Krycer, J., James, D., O'Donoghue, S. (2015). SnapShot: Insulin/IGF1 Signaling. Cell, 161(4), 948-948. [More Information]
  • Hocking, S., Stewart, R., Brandon, A., Suryana, E., Stuart, E., Baldwin, E., Kolumam, G., Modrusan, Z., Junutula, J., Gunton, J., James, D., et al (2015). Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and inflammation in mice. Diabetologia, 58(7), 1587-1600. [More Information]
  • Parker, B., Yang, G., Humphrey, S., Chaudhuri, R., Ma, X., Peterman, S., James, D. (2015). Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Science Signaling, 8(380), 1-8. [More Information]
  • Stoeckli, J., Meoli, C., Hoffman, N., Fazakerley, D., Pant, H., Cleasby, M., Ma, X., Kleinert, M., Brandon, A., Lopez, J., James, D., et al (2015). The RabGAP TBC1D1 Plays a Central Role in Exercise-Regulated Glucose Metabolism in Skeletal Muscle. Diabetes, 64(6), 1914-1922. [More Information]
  • Kleinert, M., Sylow Hansen, L., Fazakerley, D., Krycer, J., Thomas, K., Oxbøll, A., Jordy, A., Jensen, T., Yang, G., Schjerling, P., James, D., et al (2014). Acute mTOR inhibition induces insulin resistance and alters substrate utilization in vivo. Molecular Metabolism, 3(6), 630-641. [More Information]
  • Yang, P., Patrick, E., Tan, S., Fazakerley, D., Burchfield, J., Gribben, C., Prior, M., James, D., Yang, J. (2014). Direction pathway analysis of large-scale proteomics data reveals novel features of the insulin action pathway. Bioinformatics, 30(6), 808-814. [More Information]
  • Li, J., Cantley, J., Burchfield, J., Meoli, C., Stockli, J., Whitworth, P., Pant, H., Chaudhuri, R., Groffen, A., Verhage, M., James, D. (2014). DOC2 isoforms play dual roles in insulin secretion and insulin-stimulated glucose uptake. Diabetologia, 57(10), 2173-2182. [More Information]
  • Chow, J., Lawrence, R., Healy, M., Dominy, J., Liao, J., Breen, D., Byrne, F., Kenwood, B., Lackner, C., James, D., et al (2014). Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylation. Molecular Metabolism, 3(4), 419-431. [More Information]
  • Wu, L., Samocha-Bonet, D., Whitworth, P., Fazakerley, D., Turner, N., Biden, T., James, D., Cantley, J. (2014). Identification of fatty acid binding protein 4 as an adipokine that regulates insulin secretion during obesity. Molecular Metabolism, 3(4), 465-473. [More Information]
  • Ma, X., Yang, P., Kaplan, W., Lee, B., Wu, L., Yang, J., Yasunaga, M., Sato, K., Chisholm, D., James, D. (2014). ISL1 Regulates Peroxisome Proliferator-Activated Receptor gamma Activation and Early Adipogenesis via Bone Morphogenetic Protein 4-Dependent and -Independent Mechanisms. Molecular and Cellular Biology, 34(19), 3607-3617. [More Information]
  • Taddeo, E., Laker, R., Breen, D., Akhtar, Y., Kenwood, B., Liao, J., Zhang, M., Fazakerley, D., Tomsig, J., Harris, T., James, D., et al (2014). Opening of the mitochondrial permeability transition pore links mitochondrial dysfunction to insulin resistance in skeletal muscle. Molecular Metabolism, 3(2), 124-134. [More Information]
  • Lee, J., Walsh, M., Hoehn, K., James, D., Wherry, E., Choi, Y. (2014). Regulator of Fatty Acid metabolism, acetyl coenzyme a carboxylase 1, controls T cell immunity. The Journal of Immunology, 192(7), 3190-3199. [More Information]
  • Parker, B., Shepherd, N., Trefely, S., Hoffman, N., White, M., Engholm-Keller, K., Hambly, B., Larsen, M., James, D., Cordwell, S. (2014). Structural Basis for Phosphorylation and Lysine Acetylation Cross-talk in a Kinase Motif Associated with Myocardial Ischemia and Cardioprotection. The Journal of Biological Chemistry, 289(37), 25890-25906. [More Information]
  • Wu, L., Meoli, C., Mangiafico, S., Fazakerley, D., Cogger, V., Mohamad, M., Pant, H., Kang, M., Powter, E., Burchfield, J., Gamble, J., Le Couteur, D., James, D., et al (2014). Systemic VEGF-A Neutralization Ameliorates Diet-Induced Metabolic Dysfunction. Diabetes, 63(8), 2656-2667. [More Information]
  • Fletcher, R., Gribben, C., Ma, X., Burchfield, J., Thomas, K., Krycer, J., James, D., Fazakerley, D. (2014). The Role of the Niemann-Pick Disease, Type C1 Protein in Adipocyte Insulin Action. PloS One, 9(4), 1-10. [More Information]
  • Iseli, T., Turner, N., Zeng, X., Cooney, G., Kraegen, E., Yao, S., Ye, Y., James, D., Ye, J. (2013). Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKKbeta. PloS One, 8(4), 1-9. [More Information]
  • Yu, H., Rathore, S., Lopez, J., Davis, E., James, D., Martin, J., Shen, J. (2013). Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 110(35), E3271-E3280. [More Information]
  • Humphrey, S., Yang, G., Yang, P., Fazakerley, D., Stockli, J., Yang, J., James, D. (2013). Dynamic adipocyte phosphoproteome reveals that akt directly regulates mTORC2. Cell Metabolism, 17(6), 1009-1020. [More Information]
  • Shewan, A., McCann, R., Lamb, C., Stirrat, L., Kioumourtzoglou, D., Adamson, I., Verma, S., James, D., Bryant, N. (2013). Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells. Journal of Cell Science, 126(7), 1576-1582. [More Information]
  • Tonks, K., Ng, Y., Miller, S., Coster, A., Samocha-Bonet, D., Iseli, T., Xu, A., Patrick, E., Yang, J., Junutula, J., et al (2013). Impaired Akt phosphorylation in insulin-resistant human muscle is accompanied by selective and heterogeneous downstream defects. Diabetologia, 56(4), 875-885. [More Information]
  • Burchfield, J., Lu, J., Fazakerley, D., Tan, S., Ng, Y., Mele, K., Buckley, M., Han, W., Hughes, W., James, D. (2013). Novel systems for dynamically assessing insulin action in live cells reveals heterogeneity in the insulin response. Traffic, 14(3), 259-273. [More Information]
  • Xie, L., Yang, Y., Liang, T., Dolai, S., Xie, H., Parsaud, L., Lopez, J., He, Y., Chidambaram, S., Lam, P., et al (2013). RalA GTPase Tethers Insulin Granules to L- and R-Type Calcium Channels Through Binding α2δ-1 Subunit. Traffic, 14(4), 428-439. [More Information]
  • Tan, S., Ng, Y., Meoli, C., Kumar, A., Khoo, P., Fazakerley, D., Junutula, J., Vali, S., James, D., Stockli, J. (2012). Amplification and demultiplexing in insulin-regulated Akt protein kinase pathway in adipocytes. The Journal of Biological Chemistry, 287(9), 6128-6138. [More Information]
  • Christie, M., Whitten, A., King, G., Hu, S., Jarrott, R., Chen, K., Duff, A., Callow, P., Collins, B., James, D., et al (2012). Low-resolution solution structures of Munc 18: Syntaxin protein complexes indicate an open binding mode driven by the Syntaxin N-peptide. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 109(25), 9816-9821. [More Information]
  • King, G., Stockli, J., Hu, S., Winnen, B., Duprez, W., Meoli, C., Junutula, J., Jarrott, R., James, D., Whitten, A., et al (2012). Membrane-curvature protein exhibits interdomain flexibility and binds a small GTPase. The Journal of Biological Chemistry, 287(49), 40996-41006. [More Information]
  • Hoehn, K., Turner, N., Cooney, G., James, D. (2012). Phenotypic Discrepancies in Acetyl-CoA Carboxylase 2-deficient Mice. Journal of Biological Chemistry, 287(19), 15801. [More Information]
  • Yang, P., Humphrey, S., Fazakerley, D., Prior, M., Yang, G., James, D., Yang, J. (2012). Re-Fraction: A machine learning approach for deterministic identification of protein homologues and splice variants in large-scale MS-based proteomics. Journal of Proteome Research, 11(5), 3035-3045. [More Information]
  • Davey, J., Humphrey, S., Junutula, J., Mishra, A., Lambright, D., James, D., Stockli, J. (2012). TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic, 13(10), 1429-1441. [More Information]
  • Tan, S., Ng, Y., Burchfield, J., Ramm, G., Lambright, D., Stockli, J., James, D. (2012). The RabGAP TBC1D4/AS160 contains an atypical PTB domain that interacts with plasma membrane phospholipids to facilitae GLUT4 trafficking in adipocytes. Molecular and Cellular Biology, 32(24), 4946-4959. [More Information]
  • Hu, S., Whitten, A., King, G., Jones, A., Rowland, A., James, D., Martin, J. (2012). The weak complex between RhoGAP Protein ARHGAP22 and Signal Regulatory Protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PloS One, 7(8), 1-11. [More Information]
  • Humphrey, S., James, D. (2012). Uncaging Akt. Science Signaling, 5(223). [More Information]
  • Stockli, J., Fazakerley, D., James, D. (2011). GLUT4 exocytosis. Journal of Cell Science, 124(24), 4147-4159. [More Information]
  • Rowland, A., Larance, M., Hughes, W., James, D. (2011). Identification of RhoGAP22 as an Akt-Dependent Regulator of Cell Motility in Response to Insulin. Molecular and Cellular Biology, 31(23), 4789-4800. [More Information]
  • Rowland, A., Fazakerley, D., James, D. (2011). Mapping Insulin/GLUT4 Circuitry. Traffic, 12(6), 672-681. [More Information]
  • Tan, S., Ng, Y., James, D. (2011). Next-generation Akt inhibitors provide greater specificity: effects on glucose metabolism in adipocytes. The Biochemical Journal, 435(2), 539-544. [More Information]
  • Prior, M., Larance, M., Lawrence, R., Soul, J., Humphrey, S., Burchfield, J., Kistler, C., Davey, J., La-Borde, P., Buckley, M., James, D., et al (2011). Quantitative Proteomic Analysis of the Adipocyte Plasma Membrane. Journal of Proteome Research, 10(11), 4970-4982. [More Information]
  • Bach, M., Larance, M., James, D., Ramm, G. (2011). The serine/threonine kinase ULK1 is a target of multiple phosphorylation events. The Biochemical Journal, 440(2), 283-291. [More Information]
  • Konstantopoulos, N., Foletta, V., Segal, D., Shields, K., Sanigorski, A., Windmill, K., Swinton, C., Connor, T., Wanyonyi, S., Dyer, T., et al (2010). A Gene Expression Signature for Insulin Resistance. Physiological Genomics, 43(3), 110-20. [More Information]
  • Hoehn, K., Turner, N., Swarbrick, M., Wilks, D., Preston, E., Phua, Y., Hoy, A., James, D., Cooney, G., et al (2010). Acute or chronic upregulation of mitochondrial fatty acid oxidation has no net effect on whole-body energy expenditure or adiposity. Cell Metabolism, 11(1), 70-76. [More Information]
  • Tan, S., Ng, Y., James, D. (2010). Akt inhibitors reduce glucose uptake independently of their effects on Akt. Biochemical Journal, 432(1), 191-197. [More Information]
  • Ng, Y., Ramm, G., Burchfield, J., Coster, A., Stockli, J., James, D. (2010). Cluster Analysis of Insulin Action in Adipocytes Reveals a Key Role for Akt at the Plasma Membrane. Journal of Biological Chemistry, 285(4), 2245-2257. [More Information]
  • Ng, Y., Ramm, G., James, D. (2010). Dissecting the mechanism of insulin resistance using a novel heterodimerization strategy to activate Akt. Journal of Biological Chemistry, 285(8), 5232-5239. [More Information]
  • Larance, M., Rowland, A., Hoehn, K., Humphreys, D., Preiss, T., Guilhaus, M., James, D. (2010). Global phosphoproteomics identifies a major role for AKT and 14-3-3 in regulating EDC3. Molecular and Cellular Proteomics, 9(4), 682-694. [More Information]
  • Lamb, C., McCann, R., Stockli, J., James, D., Bryant, N. (2010). Insulin-Regulated Trafficking of GLUT4 Requires Ubiquitination. Traffic (Malden), 11, 1445-54. [More Information]
  • Hocking, S., Wu, L., Guilhaus, M., Chisholm, D., James, D. (2010). Intrinsic depot-specific differences in secretome of adipose tissue, preadipocytes and adipose tissue derived microvascular endothelial cells. Diabetes, 59(12), 3008-3016. [More Information]
  • Fazakerley, D., Holman, G., Marley, A., James, D., Stockli, J., Coster, A. (2010). Kinetic evidence for unique regulation of GLUT4 trafficking by insulin and AMP-activated protein kinase activators in L6 myotubes. Journal of Biological Chemistry, 285(3), 1653-1660. [More Information]
  • Stockli, J., Fazakerley, D., Coster, A., Holman, G., James, D. (2010). Muscling in on GLUT4 kinetics. Communicative and Integrative Biology, 3(3), 260-262. [More Information]
  • Lopez, J., Burchfield, J., Blair, D., Mele, K., Ng, Y., Vallotton, P., James, D., Hughes, W. (2009). Identification of a Distal GLUT4 Trafficking Event Controlled by Actin Polymerisation. Molecular Biology of the Cell, 20(17), 3918-3929. [More Information]
  • Stockli, J., James, D. (2009). Insulin Action under Arrestin. Cell Metabolism, 9(3), 213-214. [More Information]
  • Hoehn, K., Salmon, A., Hohnen-Behrens, C., Turner, N., Hoy, A., Maghzal, G., Stocker, R., Van Remmen, H., Kraegen, E., Cooney, G., et al (2009). Insulin resistance is a cellular antioxidant defense mechanism. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 106(42), 17787-17792. [More Information]
  • Sun, C., Vallotton, P., Wang, D., Lopez, J., Ng, Y., James, D. (2009). Membrane boundary extraction using circular multiple paths. Air Medical Journal, 42(4), 523-530. [More Information]
  • Crowe, S., Wu, L., Economou, C., Turpin, S., Matzaris, M., Hoehn, K., Hevener, A., James, D., Duh, E., et al (2009). Pigment epithelium-derived factor contributes to insulin resistance in obesity. Cell Metabolism, 10(1), 40-47. [More Information]
  • Zhao, P., Yang, L., Lopez, J., Fan, J., Burchfield, J., Bai, L., Hong, W., Xu, T., James, D. (2009). Variations in the requirement for v-SNAREs in GLUT4 trafficking in adipocytes. Journal of Cell Science, 122(19), 3472-3480. [More Information]
  • Tan, M., Ye, J., Turner, N., Hohnen-Behrens, C., Ke, C., Tang, C., Chen, T., Weiss, H., Gesing, E., Rowland, A., et al (2008). Anti-diabetic activities of triterpenoids from bitter melon associated with activation of the AMPK pathway. Chemistry and Biology, 15(3), 263-273. [More Information]
  • Turner, N., Li, J., Gosby, A., To, S., Cheng, Z., Miyoshi, H., Taketo, M., Cooney, G., Kraegen, E., James, D., et al (2008). Berberine and its more Biologically Availible Derivative Dihyroberberine, Inhibit Mitochondrial Respiratory Complex I: A Mechanism for the Action of Berberine to Activate AMPK and Improve Insulin Action. Diabetes, 57(5), 1414-1418. [More Information]
  • Yip, M., Ramm, G., Larance, M., Hoehn, K., Wagner, M., Guilhaus, M., James, D. (2008). CaMKII-Mediated Phosphorylation of the Myosin Motor Myo1c is required for Insulin-Stimulated GLUT4 Translocation in Adipocytes. Cell Metabolism, 8(5), 384-398. [More Information]
  • Hoehn, K., Hohnen-Behrens, C., Cederberg, A., Wu, L., Turner, N., Yuasa, T., Ebina, Y., James, D. (2008). IRS1-independent defects define major nodes of insulin resistance. Cell Metabolism, 7(5), 421-433. [More Information]
  • Brummer, T., Larance, M., Abreu, M., Lyons, R., Timpson, P., Emmerich, C., Fleuren, E., Lehrbach, G., Schramek, D., Guilhaus, M., et al (2008). Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein. The EMBO Journal, 27(17), 2305-2316. [More Information]
  • Ng, Y., Ramm, G., Lopez, J., James, D. (2008). Rapid Activation of Akt2 is Sufficient to Stimulate GLUT4 Translocation in 3T3-L1 Adipocytes. Cell Metabolism, 7(4), 348-356. [More Information]
  • Stockli, J., Davey, J., Hohnen-Behrens, C., Xu, A., James, D., Ramm, G. (2008). Regulation of Glut4 Translocation by the RabGAP AS160/TBC1D4: Role of Phosphorylation and Membrane Association. Molecular Endocrinology, 22(12), 2703-2715. [More Information]
  • Achuthan, A., Masendycz, P., Lopez, J., Nguyen, T., James, D., Sweet, M., Hamilton, J., Scholz, G. (2008). Regulation of the Endosomal SNARE Protein Syntaxin 7 by Colony-Stimulating Factor 1 in Macrophages. Molecular and Cellular Biology, 28(20), 6149-6159. [More Information]
  • Bao, Y., Lopez, J., James, D., Hunziker, W. (2008). Snapin Interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking. Journal of Biological Chemistry, 283(1), 324-331. [More Information]
  • Hocking, S., Chisholm, D., James, D. (2008). Studies of Regional adipose transplantation reveal a unique and beneficial interaction between subcutaneous adipose tissue and the intra-abdominal compartment. Diabetologia, 51(5), 900-902. [More Information]
  • Larance, M., Ramm, G., James, D. (2008). The GLUT4 Code. Molecular Endocrinology, 22(2), 226-233. [More Information]
  • Lopez, J., Kwan, E., Xie, L., He, Y., James, D., Gaisano, H. (2008). The RalA GTPase is a Central Regulator of Insulin Exocytosis for Pancreatic Islet Beta Cells. The Journal of Biological Chemistry, 283(26), 17939-17945. [More Information]
  • Hoy, A., Bruce, C., Cederberg, A., Turner, N., James, D., Cooney, G., Kraegen, E. (2007). Glucose infusion causes insulin resistance in skeletal muscle of rats without changes in Akt and AS160 phosphorylation. American Journal of Physiology: Endocrinology and Metabolism, 293(5), e1358-e1364. [More Information]

Conferences

  • Mele, K., Vallotton, P., Burchfield, J., James, D., Hughes, W. (2009). Towards fully automated identification of Vesicle-Membrane Fusion Events in TIRF Microscopy. [More Information]

Patents

  • Hu, L., Ye, J., Li, J., James, D., Kraegen, E., Zhang, H., Cheng, Z., Li, J. (2010). 13, 13a-Dihydroberberine derivatives, their pharmaceutical compositions and uses. Patent No. 2007816496.
  • Ke, C., James, D., Kraegen, E., Cooney, G., Ye, Y., Tan, M., Chen, T., Li, X., Ye, J. (2009). Use of compounds extracted from Momordica charantia L. in the manufacture of medicaments for prevention and treatment of diabetes and obesity. Patent No. 2009212026.

2016

  • Schoenwaelder, S., Darbousset, R., Cranmer, S., Ramshaw, H., Orive, S., Sturgeon, S., Yuan, Y., Yao, Y., Krycer, J., Woodcock, J., Maclean, J., James, D., Jackson, S., et al (2016). 14-3-3z regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function. Nature Communications, 7, 1-15. [More Information]
  • Wu, L., Levina, A., Harris, H., Cai, Z., Lai, B., Vogt, S., James, D., Lay, P. (2016). Carcinogenic chromium(VI) compounds formed by intracellular oxidation of chromium(III) dietary supplements by adipocytes. Angewandte Chemie: International Edition, 55(5), 1742-1745. [More Information]
  • Solon-Biet, S., Cogger, V., Pulpitel, T., Heblinski, M., Wahl, D., McMahon, A., Warren, A., Durrant-Whyte, J., Walters, K., Krycer, J., Ponton, F., Gokarn, R., Wali, J., Conigrave, A., James, D., Raubenheimer, D., Le Couteur, D., Simpson, S., et al (2016). Defining the Nutritional and Metabolic Context of FGF21 Using the Geometric Framework. Cell Metabolism, 24(4), 555-565. [More Information]
  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Stockli, J., James, D. (2016). Hyper-activation of the insulin signaling pathway improves intracellular proteostasis by coordinately upregulating the proteostatic machinery in adipocytes. Journal of Biological Chemistry, in press. [More Information]
  • Davey, J., Watt, K., Parker, B., Chaudhuri, R., Ryall, J., Cunningham, L., Qian, H., Sartorelli, V., Sandri, M., Chamberlain, J., James, D., et al (2016). Integrated expression analysis of muscle hypertrophy identifies Asb2 as a negative regulator of muscle mass. JCI Insight, 1(5), e85477. [More Information]
  • Yang, P., Patrick, E., Humphrey, S., Ghazanfar, S., James, D., Jothi, R., Yang, J. (2016). KinasePA: Phosphoproteomics data annotation using hypothesis driven kinase perturbation analysis. Proteomics, 16(13), 1868-1871. [More Information]
  • Brandon, A., Stuart, E., Leslie, S., Hoehn, K., James, D., Kraegen, E., Turner, N., Cooney, G. (2016). Minimal impact of age and housing temperature on the metabolic phenotype of Acc2K/K mice. Journal of Endocrinology, 228(3), 127-134. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stockli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17, 29-36. [More Information]
  • Kleinert, M., Parker, B., Chaudhuri, R., Fazakerley, D., Serup, A., Thomas, K., Krycer, J., Sylow Hansen, L., Fritzen, A., Hoffman, N., James, D., et al (2016). mTORC2 and AMPK differentially regulate muscle triglyceride content via Perilipin 3. Molecular Metabolism, 5(8), 646-655. [More Information]
  • Vafaee, F., Krycer, J., Ma, X., Burykin, T., James, D., Kuncic, Z. (2016). ORTI: An Open-Access Repository of Transcriptional Interactions for Interrogating Mammalian Gene Expression Data. PloS One, 11(10), 1-21. [More Information]
  • Yang, P., Humphrey, S., James, D., Yang, J., Jothi, R. (2016). Positive-unlabeled ensemble learning for kinase substrate prediction from dynamic phosphoproteomics data. Bioinformatics, 32(2), 252-259. [More Information]
  • Rogers, S., Fey, D., McCloy, R., Parker, B., Mitchell, N., Payne, R., Daly, R., James, D., Caldon, C., Watkins, D., et al (2016). PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells. Journal of Cell Science, 129(7), 1340-1354. [More Information]
  • Tonks, K., Coster, A., Christopher, M., Chaudhuri, R., Xu, A., Gagnon-Bartsch, J., Chisholm, D., James, D., Meikle, P., Greenfield, J., et al (2016). Skeletal Muscle and Plasma Lipidomic Signatures of Insulin Resistance and Overweight/Obesity in Humans. Obesity, 24(4), 908-916. [More Information]
  • Parker, B., Thaysen-Andersen, M., Fazakerley, D., Holliday, M., Packer, N., James, D. (2016). Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes. Molecular and Cellular Proteomics, 15(1), 141-153. [More Information]
  • Domanova, W., Krycer, J., Chaudhuri, R., Yang, P., Vafaee, F., Fazakerley, D., Humphrey, S., James, D., Kuncic, Z. (2016). Unraveling Kinase Activation Dynamics Using Kinase-Substrate Relationships from Temporal Large-Scale Phosphoproteomics Studies. PloS One, 11(6), 1-14. [More Information]

2015

  • Wong, M., Krycer, J., Burchfield, J., James, D., Kuncic, Z. (2015). A generalised enzyme kinetic model for predicting the behaviour of complex biochemical systems. FEBS Open Bio, 5, 226-239. [More Information]
  • Yang, G., Murashige, D., Humphrey, S., James, D. (2015). A Positive Feedback Loop between Akt and mTORC2 via SIN1 Phosphorylation. Cell Reports, 12(6), 937-943. [More Information]
  • Lee, J., Walsh, M., Hoehn, K., James, D., Wherry, E., Choi, Y. (2015). Acetyl CoA Carboxylase 2 Is Dispensable for CD8+ T Cell Responses. PloS One, 10(9), 1-9. [More Information]
  • Kee, A., Yang, L., Lucas, C., Greenberg, M., Martel, N., Leong, G., Hughes, W., Cooney, G., James, D., Ostap, M., et al (2015). An Actin Filament Population Defined by the Tropomyosin Tpm3.1 Regulates Glucose Uptake. Traffic, 16(7), 691-711. [More Information]
  • Ma, X., Lee, P., Chisholm, D., James, D. (2015). Control of adipocyte differentiation in different fat depots; Implications for pathophysiology or therapy. Frontiers in Endocrinology, 6, 1-8. [More Information]
  • Chaudhuri, R., Khoo, P., Tonks, K., Junutula, J., Kolumam, G., Modrusan, Z., Samocha-Bonet, D., Meoli, C., Hocking, S., Fazakerley, D., Stockli, J., Yang, J., James, D., et al (2015). Cross-species gene expression analysis identifies a novel set of genes implicated in human insulin sensitivity. Npj Systems Biology And Applications, 1, 1-10. [More Information]
  • Rogers, S., McCloy, R., Parker, B., Chaudhuri, R., Gayevskiy, V., Hoffman, N., Watkins, D., Daly, R., James, D., Burgess, A. (2015). Dataset from the global phosphoproteomic mapping of early mitotic exit in human cells. Data in Brief, 5, 45-52. [More Information]
  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • McCloy, R., Parker, B., Rogers, S., Chaudhuri, R., Gayevskiy, V., Hoffman, N., Ali, N., Watkins, N., Daly, R., James, D., et al (2015). Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs. Molecular and Cellular Proteomics, 14(8), 2194-2212. [More Information]
  • Trefely, S., Khoo, P., Krycer, J., Chaudhuri, R., Fazakerley, D., Parker, B., Sultani, G., Lee, J., Stephan, J., Torres, E., James, D., Stoeckli, J., et al (2015). Kinome Screen Identifies PFKFB3 and Glucose Metabolism as Important Regulators of the Insulin/IGF-1 Signalling Pathway. The Journal of Biological Chemistry, 290(43), 25834-25846. [More Information]
  • Phua, Y., Nguyen, A., Roden, D., Elsworth, B., Deng, N., Nikolic, I., Yang, J., McFarland, A., Russell, R., Kaplan, W., O'Toole, S., James, D., et al (2015). MicroRNA profiling of the pubertal mouse mammary gland identifies miR-184 as a candidate breast tumour suppressor gene. Breast Cancer Research, 17(1), 1-18. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Humphrey, S., James, D., Mann, M. (2015). Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation. Trends in Endocrinology and Metabolism, 26(12), 676-687. [More Information]
  • Fazakerley, D., Naghiloo, S., Chaudhuri, R., Koumanov, F., Burchfield, J., Thomas, K., Krycer, J., Prior, M., Parker, B., Murrow, B., Stoeckli, J., Meoli, C., James, D., et al (2015). Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumour Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin Action in Adipocytes. The Journal of Biological Chemistry, 290(39), 23528-23542. [More Information]
  • Tan, S., Fisher_Wellman, K., Fazakerley, D., Ng, Y., Pant, H., Li, J., Meoli, C., Coster, A., Stoeckli, J., James, D. (2015). Selective insulin resistance in adipocytes. The Journal of Biological Chemistry, 290(18), 11337-11348. [More Information]
  • Ma, D., Stolte, C., Krycer, J., James, D., O'Donoghue, S. (2015). SnapShot: Insulin/IGF1 Signaling. Cell, 161(4), 948-948. [More Information]
  • Hocking, S., Stewart, R., Brandon, A., Suryana, E., Stuart, E., Baldwin, E., Kolumam, G., Modrusan, Z., Junutula, J., Gunton, J., James, D., et al (2015). Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and inflammation in mice. Diabetologia, 58(7), 1587-1600. [More Information]
  • Parker, B., Yang, G., Humphrey, S., Chaudhuri, R., Ma, X., Peterman, S., James, D. (2015). Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Science Signaling, 8(380), 1-8. [More Information]
  • Stoeckli, J., Meoli, C., Hoffman, N., Fazakerley, D., Pant, H., Cleasby, M., Ma, X., Kleinert, M., Brandon, A., Lopez, J., James, D., et al (2015). The RabGAP TBC1D1 Plays a Central Role in Exercise-Regulated Glucose Metabolism in Skeletal Muscle. Diabetes, 64(6), 1914-1922. [More Information]

2014

  • Kleinert, M., Sylow Hansen, L., Fazakerley, D., Krycer, J., Thomas, K., Oxbøll, A., Jordy, A., Jensen, T., Yang, G., Schjerling, P., James, D., et al (2014). Acute mTOR inhibition induces insulin resistance and alters substrate utilization in vivo. Molecular Metabolism, 3(6), 630-641. [More Information]
  • Yang, P., Patrick, E., Tan, S., Fazakerley, D., Burchfield, J., Gribben, C., Prior, M., James, D., Yang, J. (2014). Direction pathway analysis of large-scale proteomics data reveals novel features of the insulin action pathway. Bioinformatics, 30(6), 808-814. [More Information]
  • Li, J., Cantley, J., Burchfield, J., Meoli, C., Stockli, J., Whitworth, P., Pant, H., Chaudhuri, R., Groffen, A., Verhage, M., James, D. (2014). DOC2 isoforms play dual roles in insulin secretion and insulin-stimulated glucose uptake. Diabetologia, 57(10), 2173-2182. [More Information]
  • Chow, J., Lawrence, R., Healy, M., Dominy, J., Liao, J., Breen, D., Byrne, F., Kenwood, B., Lackner, C., James, D., et al (2014). Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylation. Molecular Metabolism, 3(4), 419-431. [More Information]
  • Wu, L., Samocha-Bonet, D., Whitworth, P., Fazakerley, D., Turner, N., Biden, T., James, D., Cantley, J. (2014). Identification of fatty acid binding protein 4 as an adipokine that regulates insulin secretion during obesity. Molecular Metabolism, 3(4), 465-473. [More Information]
  • Ma, X., Yang, P., Kaplan, W., Lee, B., Wu, L., Yang, J., Yasunaga, M., Sato, K., Chisholm, D., James, D. (2014). ISL1 Regulates Peroxisome Proliferator-Activated Receptor gamma Activation and Early Adipogenesis via Bone Morphogenetic Protein 4-Dependent and -Independent Mechanisms. Molecular and Cellular Biology, 34(19), 3607-3617. [More Information]
  • Taddeo, E., Laker, R., Breen, D., Akhtar, Y., Kenwood, B., Liao, J., Zhang, M., Fazakerley, D., Tomsig, J., Harris, T., James, D., et al (2014). Opening of the mitochondrial permeability transition pore links mitochondrial dysfunction to insulin resistance in skeletal muscle. Molecular Metabolism, 3(2), 124-134. [More Information]
  • Lee, J., Walsh, M., Hoehn, K., James, D., Wherry, E., Choi, Y. (2014). Regulator of Fatty Acid metabolism, acetyl coenzyme a carboxylase 1, controls T cell immunity. The Journal of Immunology, 192(7), 3190-3199. [More Information]
  • Parker, B., Shepherd, N., Trefely, S., Hoffman, N., White, M., Engholm-Keller, K., Hambly, B., Larsen, M., James, D., Cordwell, S. (2014). Structural Basis for Phosphorylation and Lysine Acetylation Cross-talk in a Kinase Motif Associated with Myocardial Ischemia and Cardioprotection. The Journal of Biological Chemistry, 289(37), 25890-25906. [More Information]
  • Wu, L., Meoli, C., Mangiafico, S., Fazakerley, D., Cogger, V., Mohamad, M., Pant, H., Kang, M., Powter, E., Burchfield, J., Gamble, J., Le Couteur, D., James, D., et al (2014). Systemic VEGF-A Neutralization Ameliorates Diet-Induced Metabolic Dysfunction. Diabetes, 63(8), 2656-2667. [More Information]
  • Fletcher, R., Gribben, C., Ma, X., Burchfield, J., Thomas, K., Krycer, J., James, D., Fazakerley, D. (2014). The Role of the Niemann-Pick Disease, Type C1 Protein in Adipocyte Insulin Action. PloS One, 9(4), 1-10. [More Information]

2013

  • Iseli, T., Turner, N., Zeng, X., Cooney, G., Kraegen, E., Yao, S., Ye, Y., James, D., Ye, J. (2013). Activation of AMPK by Bitter Melon Triterpenoids Involves CaMKKbeta. PloS One, 8(4), 1-9. [More Information]
  • Yu, H., Rathore, S., Lopez, J., Davis, E., James, D., Martin, J., Shen, J. (2013). Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 110(35), E3271-E3280. [More Information]
  • Humphrey, S., Yang, G., Yang, P., Fazakerley, D., Stockli, J., Yang, J., James, D. (2013). Dynamic adipocyte phosphoproteome reveals that akt directly regulates mTORC2. Cell Metabolism, 17(6), 1009-1020. [More Information]
  • Shewan, A., McCann, R., Lamb, C., Stirrat, L., Kioumourtzoglou, D., Adamson, I., Verma, S., James, D., Bryant, N. (2013). Endosomal sorting of GLUT4 and Gap1 is conserved between yeast and insulin-sensitive cells. Journal of Cell Science, 126(7), 1576-1582. [More Information]
  • Tonks, K., Ng, Y., Miller, S., Coster, A., Samocha-Bonet, D., Iseli, T., Xu, A., Patrick, E., Yang, J., Junutula, J., et al (2013). Impaired Akt phosphorylation in insulin-resistant human muscle is accompanied by selective and heterogeneous downstream defects. Diabetologia, 56(4), 875-885. [More Information]
  • Burchfield, J., Lu, J., Fazakerley, D., Tan, S., Ng, Y., Mele, K., Buckley, M., Han, W., Hughes, W., James, D. (2013). Novel systems for dynamically assessing insulin action in live cells reveals heterogeneity in the insulin response. Traffic, 14(3), 259-273. [More Information]
  • Xie, L., Yang, Y., Liang, T., Dolai, S., Xie, H., Parsaud, L., Lopez, J., He, Y., Chidambaram, S., Lam, P., et al (2013). RalA GTPase Tethers Insulin Granules to L- and R-Type Calcium Channels Through Binding α2δ-1 Subunit. Traffic, 14(4), 428-439. [More Information]

2012

  • Tan, S., Ng, Y., Meoli, C., Kumar, A., Khoo, P., Fazakerley, D., Junutula, J., Vali, S., James, D., Stockli, J. (2012). Amplification and demultiplexing in insulin-regulated Akt protein kinase pathway in adipocytes. The Journal of Biological Chemistry, 287(9), 6128-6138. [More Information]
  • Christie, M., Whitten, A., King, G., Hu, S., Jarrott, R., Chen, K., Duff, A., Callow, P., Collins, B., James, D., et al (2012). Low-resolution solution structures of Munc 18: Syntaxin protein complexes indicate an open binding mode driven by the Syntaxin N-peptide. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 109(25), 9816-9821. [More Information]
  • King, G., Stockli, J., Hu, S., Winnen, B., Duprez, W., Meoli, C., Junutula, J., Jarrott, R., James, D., Whitten, A., et al (2012). Membrane-curvature protein exhibits interdomain flexibility and binds a small GTPase. The Journal of Biological Chemistry, 287(49), 40996-41006. [More Information]
  • Hoehn, K., Turner, N., Cooney, G., James, D. (2012). Phenotypic Discrepancies in Acetyl-CoA Carboxylase 2-deficient Mice. Journal of Biological Chemistry, 287(19), 15801. [More Information]
  • Yang, P., Humphrey, S., Fazakerley, D., Prior, M., Yang, G., James, D., Yang, J. (2012). Re-Fraction: A machine learning approach for deterministic identification of protein homologues and splice variants in large-scale MS-based proteomics. Journal of Proteome Research, 11(5), 3035-3045. [More Information]
  • Davey, J., Humphrey, S., Junutula, J., Mishra, A., Lambright, D., James, D., Stockli, J. (2012). TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic, 13(10), 1429-1441. [More Information]
  • Tan, S., Ng, Y., Burchfield, J., Ramm, G., Lambright, D., Stockli, J., James, D. (2012). The RabGAP TBC1D4/AS160 contains an atypical PTB domain that interacts with plasma membrane phospholipids to facilitae GLUT4 trafficking in adipocytes. Molecular and Cellular Biology, 32(24), 4946-4959. [More Information]
  • Hu, S., Whitten, A., King, G., Jones, A., Rowland, A., James, D., Martin, J. (2012). The weak complex between RhoGAP Protein ARHGAP22 and Signal Regulatory Protein 14-3-3 has 1:2 stoichiometry and a single peptide binding mode. PloS One, 7(8), 1-11. [More Information]
  • Humphrey, S., James, D. (2012). Uncaging Akt. Science Signaling, 5(223). [More Information]

2011

  • Stockli, J., Fazakerley, D., James, D. (2011). GLUT4 exocytosis. Journal of Cell Science, 124(24), 4147-4159. [More Information]
  • Rowland, A., Larance, M., Hughes, W., James, D. (2011). Identification of RhoGAP22 as an Akt-Dependent Regulator of Cell Motility in Response to Insulin. Molecular and Cellular Biology, 31(23), 4789-4800. [More Information]
  • Rowland, A., Fazakerley, D., James, D. (2011). Mapping Insulin/GLUT4 Circuitry. Traffic, 12(6), 672-681. [More Information]
  • Tan, S., Ng, Y., James, D. (2011). Next-generation Akt inhibitors provide greater specificity: effects on glucose metabolism in adipocytes. The Biochemical Journal, 435(2), 539-544. [More Information]
  • Prior, M., Larance, M., Lawrence, R., Soul, J., Humphrey, S., Burchfield, J., Kistler, C., Davey, J., La-Borde, P., Buckley, M., James, D., et al (2011). Quantitative Proteomic Analysis of the Adipocyte Plasma Membrane. Journal of Proteome Research, 10(11), 4970-4982. [More Information]
  • Bach, M., Larance, M., James, D., Ramm, G. (2011). The serine/threonine kinase ULK1 is a target of multiple phosphorylation events. The Biochemical Journal, 440(2), 283-291. [More Information]

2010

  • Hu, L., Ye, J., Li, J., James, D., Kraegen, E., Zhang, H., Cheng, Z., Li, J. (2010). 13, 13a-Dihydroberberine derivatives, their pharmaceutical compositions and uses. Patent No. 2007816496.
  • Konstantopoulos, N., Foletta, V., Segal, D., Shields, K., Sanigorski, A., Windmill, K., Swinton, C., Connor, T., Wanyonyi, S., Dyer, T., et al (2010). A Gene Expression Signature for Insulin Resistance. Physiological Genomics, 43(3), 110-20. [More Information]
  • Hoehn, K., Turner, N., Swarbrick, M., Wilks, D., Preston, E., Phua, Y., Hoy, A., James, D., Cooney, G., et al (2010). Acute or chronic upregulation of mitochondrial fatty acid oxidation has no net effect on whole-body energy expenditure or adiposity. Cell Metabolism, 11(1), 70-76. [More Information]
  • Tan, S., Ng, Y., James, D. (2010). Akt inhibitors reduce glucose uptake independently of their effects on Akt. Biochemical Journal, 432(1), 191-197. [More Information]
  • Ng, Y., Ramm, G., Burchfield, J., Coster, A., Stockli, J., James, D. (2010). Cluster Analysis of Insulin Action in Adipocytes Reveals a Key Role for Akt at the Plasma Membrane. Journal of Biological Chemistry, 285(4), 2245-2257. [More Information]
  • Ng, Y., Ramm, G., James, D. (2010). Dissecting the mechanism of insulin resistance using a novel heterodimerization strategy to activate Akt. Journal of Biological Chemistry, 285(8), 5232-5239. [More Information]
  • Larance, M., Rowland, A., Hoehn, K., Humphreys, D., Preiss, T., Guilhaus, M., James, D. (2010). Global phosphoproteomics identifies a major role for AKT and 14-3-3 in regulating EDC3. Molecular and Cellular Proteomics, 9(4), 682-694. [More Information]
  • Lamb, C., McCann, R., Stockli, J., James, D., Bryant, N. (2010). Insulin-Regulated Trafficking of GLUT4 Requires Ubiquitination. Traffic (Malden), 11, 1445-54. [More Information]
  • Hocking, S., Wu, L., Guilhaus, M., Chisholm, D., James, D. (2010). Intrinsic depot-specific differences in secretome of adipose tissue, preadipocytes and adipose tissue derived microvascular endothelial cells. Diabetes, 59(12), 3008-3016. [More Information]
  • Fazakerley, D., Holman, G., Marley, A., James, D., Stockli, J., Coster, A. (2010). Kinetic evidence for unique regulation of GLUT4 trafficking by insulin and AMP-activated protein kinase activators in L6 myotubes. Journal of Biological Chemistry, 285(3), 1653-1660. [More Information]
  • Stockli, J., Fazakerley, D., Coster, A., Holman, G., James, D. (2010). Muscling in on GLUT4 kinetics. Communicative and Integrative Biology, 3(3), 260-262. [More Information]

2009

  • Lopez, J., Burchfield, J., Blair, D., Mele, K., Ng, Y., Vallotton, P., James, D., Hughes, W. (2009). Identification of a Distal GLUT4 Trafficking Event Controlled by Actin Polymerisation. Molecular Biology of the Cell, 20(17), 3918-3929. [More Information]
  • Stockli, J., James, D. (2009). Insulin Action under Arrestin. Cell Metabolism, 9(3), 213-214. [More Information]
  • Hoehn, K., Salmon, A., Hohnen-Behrens, C., Turner, N., Hoy, A., Maghzal, G., Stocker, R., Van Remmen, H., Kraegen, E., Cooney, G., et al (2009). Insulin resistance is a cellular antioxidant defense mechanism. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 106(42), 17787-17792. [More Information]
  • Sun, C., Vallotton, P., Wang, D., Lopez, J., Ng, Y., James, D. (2009). Membrane boundary extraction using circular multiple paths. Air Medical Journal, 42(4), 523-530. [More Information]
  • Crowe, S., Wu, L., Economou, C., Turpin, S., Matzaris, M., Hoehn, K., Hevener, A., James, D., Duh, E., et al (2009). Pigment epithelium-derived factor contributes to insulin resistance in obesity. Cell Metabolism, 10(1), 40-47. [More Information]
  • Mele, K., Vallotton, P., Burchfield, J., James, D., Hughes, W. (2009). Towards fully automated identification of Vesicle-Membrane Fusion Events in TIRF Microscopy. [More Information]
  • Ke, C., James, D., Kraegen, E., Cooney, G., Ye, Y., Tan, M., Chen, T., Li, X., Ye, J. (2009). Use of compounds extracted from Momordica charantia L. in the manufacture of medicaments for prevention and treatment of diabetes and obesity. Patent No. 2009212026.
  • Zhao, P., Yang, L., Lopez, J., Fan, J., Burchfield, J., Bai, L., Hong, W., Xu, T., James, D. (2009). Variations in the requirement for v-SNAREs in GLUT4 trafficking in adipocytes. Journal of Cell Science, 122(19), 3472-3480. [More Information]

2008

  • Tan, M., Ye, J., Turner, N., Hohnen-Behrens, C., Ke, C., Tang, C., Chen, T., Weiss, H., Gesing, E., Rowland, A., et al (2008). Anti-diabetic activities of triterpenoids from bitter melon associated with activation of the AMPK pathway. Chemistry and Biology, 15(3), 263-273. [More Information]
  • Turner, N., Li, J., Gosby, A., To, S., Cheng, Z., Miyoshi, H., Taketo, M., Cooney, G., Kraegen, E., James, D., et al (2008). Berberine and its more Biologically Availible Derivative Dihyroberberine, Inhibit Mitochondrial Respiratory Complex I: A Mechanism for the Action of Berberine to Activate AMPK and Improve Insulin Action. Diabetes, 57(5), 1414-1418. [More Information]
  • Yip, M., Ramm, G., Larance, M., Hoehn, K., Wagner, M., Guilhaus, M., James, D. (2008). CaMKII-Mediated Phosphorylation of the Myosin Motor Myo1c is required for Insulin-Stimulated GLUT4 Translocation in Adipocytes. Cell Metabolism, 8(5), 384-398. [More Information]
  • Govers, R., James, D., Coster, A. (2008). High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins. In Andrei I. Ivanov (Eds.), Exocytosis and Endocytosis, (pp. 129-146). New Jersey, United States of America: Humana Press.
  • Hoehn, K., Hohnen-Behrens, C., Cederberg, A., Wu, L., Turner, N., Yuasa, T., Ebina, Y., James, D. (2008). IRS1-independent defects define major nodes of insulin resistance. Cell Metabolism, 7(5), 421-433. [More Information]
  • Brummer, T., Larance, M., Abreu, M., Lyons, R., Timpson, P., Emmerich, C., Fleuren, E., Lehrbach, G., Schramek, D., Guilhaus, M., et al (2008). Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein. The EMBO Journal, 27(17), 2305-2316. [More Information]
  • Ng, Y., Ramm, G., Lopez, J., James, D. (2008). Rapid Activation of Akt2 is Sufficient to Stimulate GLUT4 Translocation in 3T3-L1 Adipocytes. Cell Metabolism, 7(4), 348-356. [More Information]
  • Stockli, J., Davey, J., Hohnen-Behrens, C., Xu, A., James, D., Ramm, G. (2008). Regulation of Glut4 Translocation by the RabGAP AS160/TBC1D4: Role of Phosphorylation and Membrane Association. Molecular Endocrinology, 22(12), 2703-2715. [More Information]
  • Achuthan, A., Masendycz, P., Lopez, J., Nguyen, T., James, D., Sweet, M., Hamilton, J., Scholz, G. (2008). Regulation of the Endosomal SNARE Protein Syntaxin 7 by Colony-Stimulating Factor 1 in Macrophages. Molecular and Cellular Biology, 28(20), 6149-6159. [More Information]
  • Bao, Y., Lopez, J., James, D., Hunziker, W. (2008). Snapin Interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking. Journal of Biological Chemistry, 283(1), 324-331. [More Information]
  • Hocking, S., Chisholm, D., James, D. (2008). Studies of Regional adipose transplantation reveal a unique and beneficial interaction between subcutaneous adipose tissue and the intra-abdominal compartment. Diabetologia, 51(5), 900-902. [More Information]
  • Larance, M., Ramm, G., James, D. (2008). The GLUT4 Code. Molecular Endocrinology, 22(2), 226-233. [More Information]
  • Lopez, J., Kwan, E., Xie, L., He, Y., James, D., Gaisano, H. (2008). The RalA GTPase is a Central Regulator of Insulin Exocytosis for Pancreatic Islet Beta Cells. The Journal of Biological Chemistry, 283(26), 17939-17945. [More Information]

2007

  • Hoy, A., Bruce, C., Cederberg, A., Turner, N., James, D., Cooney, G., Kraegen, E. (2007). Glucose infusion causes insulin resistance in skeletal muscle of rats without changes in Akt and AS160 phosphorylation. American Journal of Physiology: Endocrinology and Metabolism, 293(5), e1358-e1364. [More Information]

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