Professor Joel Mackay

Professor

G08 - Biochemistry and Microbiology Building
The University of Sydney

Telephone + 61 2 9351 3906
Fax +61 2 9351 4726

Website Related website

Biographical details

Professor Joel Mackay is a specialist researcher in the field of protein structure, function, design and engineering.

Prof. Mackay was awarded his PhD from the University of Cambridge in 1994 and moved to The University of Sydney the following year. Since that time, Prof. Mackay has established a vibrant research laboratory in the School of Molecular Bioscience and is respected internationally for his work.

His work has focused on understanding the molecular mechanisms underlying gene regulation and erythrocyte development. He determined the first structures of protein complexes showing that zinc-finger domains can mediate protein-protein interactions. These findings revealed the molecular basis for several human genetic disorders.

Research interests

The primary focus of the Mackay group is to understand protein function at the molecular level, with a particular emphasis on three areas:

  1. the role and nature of protein-protein interactions in the control of gene expression,
  2. the structural and functional diversity of zinc-binding domains, and
  3. the design of new proteins with tailored functions.

Experimental approaches range from molecular and cell biology and biochemistry through to biophysical methods. High-quality physical infrastructure is available to support this work, including NMR spectrometers (600 and 800 MHz with cryosystems), X-ray crystallography, CD, analytical ultracentrifugation, Biacore, IT/DSC instruments, Nanotemper microscale thermophoresis, multi-angle laser light scattering, mass spectrometry and cell culture and fermentation facilities.

Teaching and supervision

Prof. Mackay contributes to third year undergraduate teaching in the area of protein structure and function.

Awards and honours

In 1997 Prof Mackay won the Biophysics Young Investigator Award and in 2001 the Roche Molecular Biochemicals Medal from the Australian Society for Biochemistry and Molecular Biology.

In 2002 he won the Science Minister's Prize for Achievement in the Life Sciences for scientists under thirty five, as well as the Australian and New Zealand Society for Magnetic Resonance Young Investigator Medal. In 2006, he was awarded the Gottschalk Medal for Medical Research from the Australian Academy of Science.

Keywords

Biochemistry; Molecular biology

Selected grants

2014

  • Structural and functional analysis of a cancer-linked co-regulator complex; Mackay J, Shepherd N, Ryan D; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Senior Research Fellowship B - Mechanisms of gene regulation - structure, function and design; Mackay J; National Health and Medical Research Council (NHMRC)/Career Awards: Research Fellowships.
  • University of Sydney Automated Peptide Synthesis Platform; Payne R, Britton W, Byrne S, Chan H, Clarke R, Hunt N, Jolliffe K, Kassiou M, Lay P, Mackay J, Matthews J, McErlean C, New E, Rasmussen H, Rendina L, Rudrawar S, Rutledge P, Shepherd N; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2013

  • Multiangle laser light scattering instrument for protein characterisation; Mackay J, Ataide S, Jormakka M, Ryan R, Matthews J, Shepherd N, Sunde M, Collyer C, Payne R; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Pulsed Electron Paramagnetic Resonance - an enhanced capability for research in quantum physics, materials science, chemistry and biological sciences; McCamey D, Moran G, Brown L, Dzurak A, Reilly D, Mackay J, Hamilton A, Lay P, Schmidt T; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • The Nanotemper: State-of-the-art instrumentation for the characterization of protein interactions; Payne R, Matthews J, Ataide S, Campbell I, Weiss A, Sunde M, Mackay J; Clive and Vera Ramaciotti Foundations/Awards for Biomedical Research: Major Equipment.

2012

  • Designer DNA-binding proteins targeting methylated DNA for research and therapeutic purposes; Mackay J, Segal D, Rasko J; National Health and Medical Research Council (NHMRC)/Project Grants.
  • The regulation of gene expression by post-translational modification of transcription factors; Blobel G, Mackay J, Blobel G; Australian Research Council (ARC)/Discovery Projects (DP).
  • Functional analysis of ZRANB2, an RNA-binding protein that is essential for mammalian development; Mackay J, Crossley M; Australian Research Council (ARC)/Discovery Projects (DP).

2011

  • Structural and functional analysis of a cancer-linked co-regulator complex; Mackay J, Blobel G; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Innovative technologies for the analysis of human gene regulation; Mackay J; DVC International/IPDF Grant.
  • POLARStar Omega; Sunde M, Guss M, Mackay J, Matthews J, Payne R, Cubeddu L; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • LMO2-containing complexes in leukemia and blood cell development; Matthews J, Mackay J, Guss M; National Health and Medical Research Council (NHMRC)/Project Grants.

2010

  • NMR Spectroscopy Facilities for the Sydney Region; Jolliffe K, Messerle B, Hambley T, Rendina L, Payne R, Mackay J, Kuchel P, Messerle B, Ball G, Price W, Crossley M; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • Designer RNA-binding proteins for research and therapeutic purposes; Mackay J, Segal D, Preiss T; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Automated Microwave accelerated Solid Phase Peptide Synthesizer; Jolliffe K, Payne R, Hambley T, Mackay J, Sunde M; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2009

  • NHMRC Senior Research Fellowship B; Mackay J; National Health and Medical Research Council (NHMRC)/Career Awards: Research Fellowships.
  • A new mechanism of gene regulation; Mackay J, Crossley P, Crossley M; Australian Research Council (ARC)/Discovery Projects (DP).
  • Structural studies of an RNA-binding protein; Mackay J; DVC Research/Bridging Support Grant.
  • Applied Biosystems 7500 Fast Real Time PCR system; Campbell I, Crossley P, Brand-Miller J, Caterson I, Hall R, Weiss A, Conigrave A, Mackay J, Matthews J, Sunde M, Gell D, Bell-Anderson K, Nicholas H, Crossett B; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Chracterisation of the cancer-related nucleosome remodelling and deacetylese complex; Mackay J; DVC Research/Cancer Research Fund.
  • New mechanisms of DNA recognition by zinc-finger domains; Mackay J; Australian Research Council (ARC)/Discovery Projects (DP).

2008

  • 800 MHz NMR spectrometer for biomolecular structure function analysis; Vadas M, Stock D, Graham R, Shine J, Ball G, Matthews J, Gell D, Trewhella J, Rutledge P, Messerle B, Fazekas B, Vandenberg J, Mackay J, Otting G, Kuchel P; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • 800 MHz NMR spectrometer for biomolecular structure-function analysis; Mackay J, Otting G, Kuchel P, Matthews J, Gell D, Trewhella J, Rutledge P, Messerle B, Vadas M, Graham R, Vandenberg J, Stock D, Ball G, Fazekas B, Shine J; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).

2007

  • Bridging Fellowship; Liew C, Mackay J; University of Sydney/Bridging Fellowship.
  • Analysis of the NuRD complex, a cancer-linked epigentic modifier; Mackay J; Cancer Institute New South Wales/Research Grant.
  • Manipulating the self assembly properties of fungal hydrophobin proteins for the design of novel bio; Sunde M, Kwan A, Mackay J; Australian Research Council (ARC)/Linkage Projects (LP).

2006

  • Regulation of gene expression: biomolecular interactions in cellular development and disease; Crossley M, Crossley P, Mackay J, Matthews J; National Health and Medical Research Council (NHMRC)/Program Grants.
  • Structure-function analysis of the splicing factor ZNF265; Mackay J, Adams D, Gell D, Adams D; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Hardware and software upgrade for the Beckman XL-A Analytical Ultracentrifuge; Mackay J; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Nuclear Magnetic Resonance work on proteins; Mackay J; BASF Aktiengesellschaft/BLO Project.
  • Structuring and Function of a New Class of Multi Zinc-Finger (mzf) proteins; Mackay J, Crossley P; Australian Research Council (ARC)/Discovery Projects (DP).

2005

  • The design of specific LM02 inhibitors; Mackay J; University of Sydney/Cancer Research Fund.
  • Characterization of a novel DNA-binding domain; Mackay J; University of Sydney/Research & Development.
  • Development of anti-T-ALL reagents; Crossley P, Mackay J, Matthews J; Leukaemia Foundation of NSW/Research Grant Program.

2004

  • Characterisation of the interactions between GATA cardiac transcription factors and T-box proteins; Mackay J; DVC Research/Research and Development Scheme: Research and Development (R&D).
  • SRFA; Mackay J; National Health and Medical Research Council (NHMRC)/Established Career Fellowships.
  • Biophysical characterisation of protein interactions within a transcription factor network; Mackay J; Australian Research Council (ARC)/Discovery Projects (DP).

2003

  • Characterization of erythroid differentiation related factor (EDRF): a novel a-globin binding protein; Mackay J, Crossley P, Gell D; Australian Research Council (ARC)/Discovery Projects (DP).
  • Understanding transcription factor interactions in blood cell development; Mackay J; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Trans-acting factors in fetal hemoglobin silencing; Crossley P, Perkins A, Mackay J; National Heart Lung and Blood Institute (USA)/Research Grant.
  • Characterization of the structure and function of TC-1, a novel tumourigenic protein; Mackay J; DVC Research/Research and Development Scheme: Research and Development (R&D).
  • 800 MHz NMR spectrometer for molecular structure-function analyses; Otting G, Mackay J, Kuchel P, Carver J, Andrews T, Young I, Board P, Keniry M, Messerle B, McCluskey A; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).

2002

  • Zinc fingers as pharmaceutical scaffolds; Crossley P, Mackay J, Matthews J; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Characterization of EDRF, a novel protein involved in globin regulation; Mackay J; DVC Research/Research and Development Scheme: Research and Development (R&D).
  • Disecting the roles of multifunctional zinc-binding domains in the regulation of gene expression; Mackay J; DVC Research/Bridging Support Grant.
  • Wyatt miniDawn EOS multiangle light scattering (MALLS) detector plus software; Mackay J, Matthews J, Crossley P; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • The dimerization of Ikaros family proteins; Mackay J; University of Sydney/Cancer Research Fund.

2001

  • The structural characterisation of zinc fingers that mediate protein-protein interacations and regulate gene expression; Mackay J, Crossley P; Australian Research Council (ARC)/Large Research Grants (LRG).

2000

  • Analysis of zinc-fingers involved in protein-protein interactions; Crossley P, Mackay J; Australian Research Council (ARC)/Large Research Grants (LRG).
  • Design of antiviral superzippers; Mackay J; Biota Respiratory Research Pty Ltd/Research Support.
  • Structural studies of protein-protein interactions controlling eukaryotic gene transcription; Australian Research Council (ARC)/Australian Research Fellowship/Queen Elizabeth II Fellowship.
  • The molecular mechanism of MOZ-mediated myeloid leukemia; Mackay J; University of Sydney/Cancer Research Fund.
  • LMO2 and Ldb1: the structure of a tumorigenic protein complex; Matthews J, Mackay J; Leo and Jenny Leukaemia and Cancer Foundation/Leukaemia and Cancer Foundation.
  • Analysis of novel zinc-binding domains in histone acetylases; Mackay J, Crossley P; Australian Research Council (ARC)/Large Research Grants (LRG).
  • Structure and function of the LAP domain from the transcriptional integrator, CBP.; Mackay J, Crossley P; Australian Research Council (ARC)/Small Grants.

1999

  • Chromatography system for protein purification; Mackay J; Clive & Vera Ramaciotti Foundation/Research Grants.

Selected publications

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Book Chapters

  • Gell, D., Mackay, J., Grant, R. (2012). The detection and quantitation of protein oligomerization. In Jacqueline M Matthews (Eds.), Protein Dimerization and Oligomerization in Biology, (pp. 19-41). New York: Springer Science + Business Media.

Journals

  • Silva, A., Kwan, A., Mackay, J. (2014). 1H, 13C and 15N resonance assignments of an N-terminal domain of CHD4. Biomolecular NMR Assignments, 8(1), 137-139. [More Information]
  • Joseph, S., Kwan, A., Mackay, J., Cubeddu, L., Matthews, J. (2014). Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1. Biomolecular NMR Assignments, 8(1), 141-144. [More Information]
  • Alqarni, S., Murthy, A., Zhanh, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 sub-complex. The Journal of Biological Chemistry, 11 June 2014, 1-22. [More Information]
  • Alqarni, S., Murthy, A., Zhang, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 subcomplex. The Journal of Biological Chemistry, 289(32), 21844-21855. [More Information]
  • Dickson, C., Kumar, K., Jacques, D., Malmirchegini, G., Spirig, T., Mackay, J., Clubb, R., Guss, M., Gell, D. (2014). Structure of the hemoglobin-IsdH complex reveals the molecular basis of iron capture by Staphylococcus aureus. The Journal of Biological Chemistry, 289(10), 6728-6738. [More Information]
  • Mackay, J. (2014). Transcription factor seeks DNA - cognate site preferred. Journal of Molecular Biology, 426(7), 1370-1372. [More Information]
  • Gamsjaeger, R., O'Connell, M., Cubeddu, L., Shepherd, N., Lowry, J., Kwan, A., Vandevenne, M., Swanton, M., Matthews, J., Mackay, J. (2013). A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers. Journal of Biological Chemistry, 288(49), 35180-35191. [More Information]
  • Dickson, C., Rich, A., D'Avigdor, W., Collins, D., Lowry, J., Mollan, T., Khandros, E., Olson, J., Weiss, M., Mackay, J., Lay, P., et al (2013). Alpha-hemoglobin-stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-alpha-hemoglobin and weakens the iron-oxygen bond. Journal of Biological Chemistry, 288(27), 19986-20001. [More Information]
  • Campbell, A., Wilkinson-White, L., Mackay, J., Matthews, J., Blobel, G. (2013). Analysis of disease-causing GATA1 mutations in murine gene complementation systems. Blood, 121(26), 5218-5227. [More Information]
  • Cordina, N., Liew, C., Gell, D., Fajer, P., Mackay, J., Brown, L. (2013). Effects of calcium binding and the hypertrophic cardiomyopathy A8V mutation on the dynamic equilibrium between closed and open conformations of the regulatory N-domain of isolated cardiac troponin C. Biochemistry, 52(11), 1950-1962. [More Information]
  • Mackay, J., Segal, D., Crossley, M. (2013). Is there a telltale RH fingerprint in zinc fingers that recognizes methylated CpG dinucleotides? Trends in Biochemical Sciences, 38(9), 421-422. [More Information]
  • Vandevenne, M., Jacques, D., Artuz, C., Nguyen, C., Kwan, A., Segal, D., Matthews, J., Crossley, M., Guss, M., Mackay, J. (2013). New insights into DNA recognition by zinc fingers revealed by structural analysis of the oncoprotein ZNF217. Journal of Biological Chemistry, 288(15), 10616-10627. [More Information]
  • Gamsjaeger, R., Kariawasam, R., Bang, L., Touma, C., Nguyen, C., Matthews, J., Cubeddu, L., Mackay, J. (2013). Semiquantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in surface plasmon resonance competition experiments. Analytical Biochemistry, 440(2), 178-185. [More Information]
  • Stokes, P., Liew, C., Kwan, A., Foo, P., Barker, H., Djamirze, A., O'Reilly, V., Visvader, J., Mackay, J., Matthews, J. (2013). Structural basis of the interaction of the breast cancer Oncogene LMO4 with the tumour suppressor CtIP/RBBP8. Journal of Molecular Biology, 425(7), 1101-1110. [More Information]
  • Liew, C., Kwan, A., Stokes, P., Mackay, J., Matthews, J. (2012). 1H, 15N and 13C assignments of an intramolecular LMO4-LIM1/CtIP complex. Biomolecular NMR Assignments, 6(1), 31-34. [More Information]
  • Bendak, K., Loughlin, F., Cheung, V., O'Connell, M., Crossley, M., Mackay, J. (2012). A rapid method for assessing the RNA-binding potential of a protein. Nucleic Acids Research, 40(14), 1-11. [More Information]
  • Morris, V., Kwan, A., Mackay, J., Sunde, M. (2012). Backbone and sidechain 1H, 13C and 15N chemical shift assignments of the hydrophobin DewA from Aspergillus nidulans. Biomolecular N M R Assignments, 8, 83-86. [More Information]
  • Musselman, C., Ramirez, J., Sims, J., Mansfield, R., Oliver, S., Denu, J., Mackay, J., Wade, P., Hagman, J., Kutateladze, T. (2012). Bivalent recognition of nucleosomes by the tandem PHD fingers of the CHD4 ATPase is required for CHD4-mediated repression. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(3), 787-792. [More Information]
  • McKenzie, J., Duyvestyn, J., Smith, T., Bendak, K., Mackay, J., Cursons, R., Cook, G., Arcus, V. (2012). Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry. RNA, 18(6), 1267-1278. [More Information]
  • Cordina, N., Liew, C., Gell, D., Fajer, P., Mackay, J., Brown, L. (2012). Interdomain orientation of cardiac Troponin C characterized by paramagnetic relaxation enhancement NMR reveals a compact state. Protein Science, 21(9), 1376-1387. [More Information]
  • O'Connell, M., Vandevenne, M., Nguyen, C., Matthews, J., Gamsjaeger, R., Segal, D., Mackay, J. (2012). Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angewandte Chemie (International Edition), 51(22), 5371-5375. [More Information]
  • Macindoe, I., Kwan, A., Ren, Q., Morris, V., Yang, W., Mackay, J., Sunde, M. (2012). Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(14), E804-E811. [More Information]
  • Dastmalchi, S., Wilkinson-White, L., Kwan, A., Gamsjaeger, R., Mackay, J., Matthews, J. (2012). Solution structure of a tethered Lmo2(LIM2)/Ldb1(LID) complex. Protein Science, 21(11), 1768-1774. [More Information]
  • Bhati, M., Lee, C., Gadd, M., Jeffries, C., Kwan, A., Whitten, A., Trewhella, J., Mackay, J., Matthews, J. (2012). Solution Structure of the LIM-Homeodomain Transcription Factor Complex Lhx3/Ldb1 and the Effects of a Pituitary Mutation on Key Lhx3 Interactions. PLoS One, 7(7), 1-9. [More Information]
  • Hsieh, Y., Wilkinson, B., O'Connell, M., Mackay, J., Matthews, J., Payne, R. (2012). Synthesis of the bacteriocin glycopeptide sublancin 168 and S-glycosylated variants. Organic Letters, 14(7), 1910-1913. [More Information]
  • Burdach, J., O'Connell, M., Mackay, J., Crossley, M. (2012). Two-timing zinc finger transcription factors liaising with RNA. Trends in Biochemical Sciences, 37(5), 199-205. [More Information]
  • Lamonica, J., Deng, W., Kadauke, S., Campbell, A., Gamsjaeger, R., Wang, H., Cheng, Y., Billin, A., Hardison, R., Mackay, J., et al (2011). Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 108(22), E159-E168. [More Information]
  • Nguyen, C., Mansfield, R., Leung, W., Vaz, P., Loughlin, F., Grant, R., Mackay, J. (2011). Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA. Journal of Molecular Biology, 407(2), 273-283. [More Information]
  • Lejon, S., Thong, S., Murthy, A., Alqarni, S., Murzina, N., Blobel, G., Laue, E., Mackay, J. (2011). Insights into Association of the NuRD Complex with FOG-1 from the Crystal Structure of an RbAp48.FOG-1 Complex. Journal of Biological Chemistry, 286(2), 1196-1203. [More Information]
  • Kwan, A., Mobli, M., Gooley, P., King, G., Mackay, J. (2011). Macromolecular NMR spectroscopy for the non-spectroscopist. The FEBS Journal, 278(5), 687-703. [More Information]
  • Bieri, M., Kwan, A., Mobli, M., King, G., Mackay, J., Gooley, P. (2011). Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination. The FEBS Journal, 278(5), 704-715. [More Information]
  • Mansfield, R., Musselman, C., Kwan, A., Oliver, S., Garske, A., Davrazou, F., Denu, J., Kutateladze, T., Mackay, J. (2011). Plant Homeodomain (PHD) Fingers of CHD4 Are Histone H3-binding Modules with Preference for Unmodified H3K4 and Methylated H3K9. Journal of Biological Chemistry, 286(13), 11779-11791. [More Information]
  • Wissmueller, S., Font Sadurni, J., Liew, C., Cram, E., Schroeder, T., Turner, J., Crossley, P., Mackay, J., Matthews, J. (2011). Protein-protein interactions: Analysis of a false positive GST pulldown result. Proteins: Structure, Function, and Bioinformatics, 79(8), 2365-2371. [More Information]
  • Gamsjaeger, R., Webb, S., Lamonica, J., Billin, A., Blobel, G., Mackay, J. (2011). Structural Basis and Specificity of Acetylated Transcription Factor GATA1 Recognition by BET Family Bromodomain Protein Brd3. Molecular and Cellular Biology, 31(13), 2632-2640. [More Information]
  • Krishna Kumar, K., Jacques, D., Pishchany, G., Caradoc-Davies, T., Spirig, T., Malmirchegini, G., Langley, D., Dickson, C., Mackay, J., Clubb, R., Guss, M., et al (2011). Structural Basis for Hemoglobin Capture by Staphylococcus aureus Cell-surface Protein, IsdH. Journal of Biological Chemistry, 286(44), 38439-38447. [More Information]
  • Wilkinson-White, L., Gamsjaeger, R., Dastmalchi, S., Wienert, B., Stokes, P., Crossley, M., Mackay, J., Matthews, J. (2011). Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 108(35), 14443-14448. [More Information]
  • Nunez Martin, N., Clifton, M., Funnell, A., Crisbel, A., Hallal, S., Quinlan (nee Edwards), K., Font Sadurni, J., Vandevenne, M., Setiyaputra, S., Pearson, R., Mackay, J., Crossley, P. (2011). The Multi-zinc Finger Protein ZNF217 Contacts DNA through a Two-finger Domain. Journal of Biological Chemistry, 286(44), 38190-38201. [More Information]
  • Mackay, J., Font Sadurni, J., Segal, D. (2011). The prospects for designer single-stranded RNA-binding proteins. Nature Structural and Molecular Biology, 18(3), 256-261. [More Information]
  • Setiyaputra, S., Mackay, J., Patrick, W. (2011). The Structure of a Truncated Phosphoribosylanthranilate Isomerase Suggests a Unified Model for Evolution of the (βα)8 Barrel Fold. Journal of Molecular Biology, 408(2), 291-303. [More Information]
  • Hynson, R., Kwan, A., Jacques, D., Mackay, J., Trewhella, J. (2010). (1)H, (13)C and (15)N backbone and side chain resonance assignments of the N-terminal domain of the histidine kinase inhibitor KipI from Bacillus subtilis. Biomolecular N M R Assignments, 4(2), 167-169. [More Information]
  • Wilkinson-White, L., Dastmalchi, S., Kwan, A., Ryan, D., Mackay, J., Matthews, J. (2010). (1)H, (15)N and (13)C assignments of an intramolecular Lmo2-LIM2/Ldb1-LID complex. Biomolecular N M R Assignments, 4(2), 203-206. [More Information]
  • Krishna Kumar, K., Dickson, C., Weiss, M., Mackay, J., Gell, D. (2010). AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state. Biochemical Journal, 432(2), 275-282. [More Information]
  • Font, J., Mackay, J. (2010). Beyond DNA: Zinc Finger Domains as RNA-Binding Modules. Methods in Molecular Biology, 649, 479-491. [More Information]
  • Schwalbe, M., Dutta, K., Libich, D., Venugopal, H., Claridge, J., Gell, D., Mackay, J., Edwards, P., Pascal, S. (2010). Two-state conformational equilibrium in the Par-4 leucine zipper domain. Proteins: Structure, Function, and Bioinformatics, 78(11), 2433-2449. [More Information]
  • Gell, D., Feng, L., Zhou, S., Jeffrey, P., Bendak, K., Gow, A., Weiss, M., Shi, Y., Mackay, J. (2009). A cis-proline in alpha-hemoglobin stabilizing protein directs the structural reorganization of alpha-hemoglobin. Journal of Biological Chemistry, 284(43), 29462-29469. [More Information]
  • Talib, J., Beck, J., Urathamakul, T., Nguyen, C., Aldrich-Wright, J., Mackay, J., Ralph, S. (2009). A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity. Chemical Communications, (37), 5546-5548. [More Information]
  • Musselman, C., Mansfield, R., Garske, A., Davrazou, F., Kwan, A., Oliver, S., O'Leary, H., Denu, J., Mackay, J., Kutateladze, T. (2009). Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications. Biochemical Journal, 423(2), 179-187. [More Information]
  • Macindoe, I., Glockner, L., Vukasin, P., Stennard, F., Costa, M., Harvey, R., Mackay, J., Sunde, M. (2009). Conformational stability and DNA binding specificity of the cardiac T-box transcription factor Tbx20. Journal of Molecular Biology, 389(3), 606-618. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2009). Corrections. Biochemistry, 48(11), 2569-2570. [More Information]
  • Matthews, J., Bhati, M., Lehtomaki, E., Mansfield, R., Cubeddu, L., Mackay, J. (2009). It Takes Two to Tango: The Structure and Function of LIM, RING, PHD and MYND Domains. Current Pharmaceutical Design, 15(31), 3681-3969. [More Information]
  • Lowry, J., Gamsjaeger, R., Thong, S., Hung, W., Kwan, A., Broitman-Maduro, G., Matthews, J., Maduro, M., Mackay, J. (2009). Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains. Journal of Biological Chemistry, 284(9), 5827-5835. [More Information]
  • O'Connell, M., Gamsjaeger, R., Mackay, J. (2009). The structural analysis of protein-protein interactions by NMR spectroscopy. Proteomics - Clinical Applications, 9, 1-9. [More Information]
  • Loughlin, F., Mansfield, R., Vaz, P., McGrath, A., Setiyaputra, S., Gamsjaeger, R., Chen, E., Morris, B., Guss, M., Mackay, J. (2009). The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 106(14), 5581-5586. [More Information]
  • Loughlin, F., Lee, M., Guss, M., Mackay, J. (2008). Crystallization of a ZRANB2-RNA complex. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(Pt 12), 1175-1177. [More Information]
  • Matthews, J., Loughlin, F., Mackay, J. (2008). Designed metal-binding sites in biomolecular and bioinorganic interactions. Current Opinion in Structural Biology, 18, 484-490. [More Information]
  • Wilkinson, S., Liew, C., Mackay, J., Salleh, H., Withers, S., McLeod, M. (2008). Escherichia coli glucuronylsynthase: An engineered enzyme for the synthesis of beta-glucuronides. Organic Letters, 10(8), 1585-1588. [More Information]
  • Wilkinson, S., Liew, C., Mackay, J., Salleh, H., Withers, S., McLeod, M. (2008). Escherichia coli Glucuronylsynthase: An Engineered Enzyme for the Synthesis of B-Glucuronides. Organic Letters, 10(8), 1585-1588. [More Information]
  • Griffin, M., Dobson, R., Pearce, F., Antonio, L., Whitten, A., Liew, C., Mackay, J., Trewhella, J., Jameson, G., Perugini, M., et al (2008). Evolution of quaternary structure in a homotetrameric enzyme. Journal of Molecular Biology, 380(4), 691-703. [More Information]
  • Bhati, M., Lee, C., Nancarrow, A., Lee, M., Craig, V., Bach, I., Guss, M., Mackay, J., Matthews, J. (2008). Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes. The EMBO Journal, 27, 2018-2029. [More Information]
  • Liew, C., Gamsjaeger, R., Mansfield, R., Mackay, J. (2008). NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins. Protein Science, 17(9), 1630-1635. [More Information]
  • Mackay, J., Lowry, J., Crossley, P., Matthews, J. (2008). Response to Chatr-aryamontri et al.: Protein interactions: to believe or not to believe? Trends in Biochemical Sciences, 33. [More Information]
  • Gamsjaeger, R., Swanton, M., Kobus, F., Lehtomaki, E., Lowry, J., Kwan, A., Matthews, J., Mackay, J. (2008). Structural and biophysical analysis of the DNA-binding properties of myelin transcription factor 1. Journal of Biological Chemistry, 283(8), 5158-5167. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2008). Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum. Biochemistry, 47(12), 3842-3854. [More Information]
  • Kwan, A., Macindoe, I., Vukasin, P., Morris, V., Kass, I., Gupte, R., Mark, A., Templeton, M., Mackay, J., Sunde, M. (2008). The Cys3-Cys4 Loop of the Hydrophobin EAS Is Not Required for Rodlet Formation and Surface Activity. Journal of Molecular Biology, 382, 708-720. [More Information]
  • Yu, X., Kong, Y., Dore, L., Abdulmalik, O., Katein, A., Zhou, S., Choi, J., Gell, D., Mackay, J., Gow, A., et al (2007). An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. Journal of Clinical Investigation, 117(7), 1856-1865. [More Information]
  • Wang, C., Guncar, G., Forwood, J., Teh, T., Catanzariti, A., Lawrence, G., Loughlin, F., Mackay, J., Schirra, H., Anderson, P., et al (2007). Crystal Structures of Flax Rust Avirulence Proteins AvrL567-A and -D Reveal Details of the Structural Basis for Flax Disease Resistance Specificity. Plant Cell, 19(9), 2898-2912. [More Information]
  • Porter, C., Matthews, J., Mackay, J., Pursglove, S., Schmidberger, J., Leedman, P., Pero, S., Krag, D., Wilce, M., Wilce, J. (2007). Grb7 SH2 domain structure and interactions with a cyclic peptide inhibitor of cancer cell migration and proliferation. B M C Structural Biology, 7(Article number 58), 1-15.
  • Kirk, E., Sunde, M., Costa, M., Rankin, S., Wolstein, O., Castro, M., Butler, T., Hyun, C., Guo, G., Otway, R., Mackay, J., Sholler, G., Winlaw, D., et al (2007). Mutations in Cardiac T-Box Factor Gene TBX20 Are Associated with Diverse Cardiac Pathologies, Including Defects of Septation and Valvulogenesis and Cardiomyopathy. American Journal of Human Genetics, 81(2), 280-291. [More Information]
  • Mackay, J., Sunde, M., Lowry, J., Crossley, P., Matthews, J. (2007). Protein interactions: is seeing believing? Trends in Biochemical Sciences, 32(12), 530-531. [More Information]
  • Gamsjaeger, R., Liew, C., Loughlin, F., Crossley, P., Mackay, J. (2007). Sticky fingers: zinc-fingers as protein-recognition motifs. Trends in Biochemical Sciences, 32(2), 63-70. [More Information]
  • Sunde, M., Kwan, A., Templeton, M., Beever, R., Mackay, J. (2007). Structural analysis of hydrophobins. Micron, doi:10.1016/j.micron.2007.08.003, 1-12. [More Information]
  • Gell, D., Westman, B., Gorman, D., Liew, C., Liew, J., Weiss, M., Mackay, J. (2006). A Novel Haem-binding Interface in the 22 kDa Haem-binding Protein p22HBP. Journal of Molecular Biology, 362(2), 287-297. [More Information]
  • Kook, H., Yung, W., Simpson, R., Kee, H., Shin, S., Lowry, J., Loughlin, F., Yin, Z., Epstein, J., Mackay, J. (2006). Analysis of the Structure and Function of the Transcriptional Coregulator HOP. Biochemistry, 45(35), 10584-10590. [More Information]
  • Lowry, J., Mackay, J. (2006). GATA-1: one protein, many partners. The International Journal of Biochemistry and Cell Biology, 38(1), 6-11. [More Information]
  • Ryan, D., Sunde, M., Kwan, A., Marianayagam, N., Nancarrow, A., vanden Hoven, R., Thompson, L., Baca, M., Mackay, J., Visvader, J., Matthews, J. (2006). Identification of the key LMO2-binding determinants on Ldb1. Journal of Molecular Biology. [More Information]
  • Liew, C., Rand, K., Simpson, R., Yung, W., Mansfield, R., Crossley, P., Proetorius-Ibba, M., Nerlov, C., Poulsen, F., Mackay, J. (2006). Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1. Journal of Biological Chemistry, 281(38), 28296-28306. [More Information]
  • Liew, C., Crossley, P., Mackay, J., Nicholas, H. (2006). Solution Structure of the THAP Domain from Caenorhabditis elegans C-terminal Binding Protein (CtBP). Journal of Molecular Biology, 366(2), 382-390. [More Information]
  • Kwan, A., Winefield, R., Sunde, M., Matthews, J., Haverkamp, R., Templeton, M., Mackay, J. (2006). Structural basis for rodlet assembly in fungal hydrophobins. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 103(10), 3621-3626. [More Information]
  • Loughlin, F., Mackay, J. (2006). Zinc fingers are known as domains for binding DNA and RNA. Do they also mediate protein-protein interactions? IUBMB Life, 58(12), 731-733. [More Information]
  • Lee, C., Nancarrow, A., Bach, I., Mackay, J., Matthews, J. (2005). 1H, 15N and 13C assignments of an intramolecular Lhx3:ldb1 complex. Journal of Biomolecular NMR, 33(3), 198-198. [More Information]
  • Duggin, I., Matthews, J., Dixon, N., Wake, R., Mackay, J. (2005). A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis. Journal of Biological Chemistry, 280(13), 13105-13113. [More Information]
  • Sharpe, B., Liew, C., Kwan, A., Wilce, J., Crossley, P., Matthews, J., Mackay, J. (2005). Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting. Structure, 13(2), 257-266. [More Information]
  • Pursglove, S., Mackay, J. (2005). CSL: A notch above the rest. The International Journal of Biochemistry and Cell Biology, 37(12), 2472-2477. [More Information]
  • Porter, C., Wilce, M., Mackay, J., Leedman, P., Wilce, J. (2005). Grb7-SH2 domain dimerisation is affected by a single point mutation. European Biophysics Journal, 34(5), 454-460. [More Information]
  • Weiss, M., Zhou, S., Feng, L., Gell, D., Mackay, J., Shi, Y., Gow, A. (2005). Role of Alpha Hemoglobin-Stabilizing Protein in Normal Erythropoiesis and {beta}-Thalassemia. New York Academy of Sciences. Annals, 1054, 103-117. [More Information]
  • Kwan, A., Fairley, K., Anderberg, P., Liew, C., Harding, M., Mackay, J. (2005). Solution structure of a recombinant type I sculpin antifreeze protein. Biochemistry, 44(6), 1980-1988. [More Information]
  • Feng, L., Zhou, S., Gu, L., Gell, D., Mackay, J., Weiss, M., Gow, A., Shi, Y. (2005). Structure of oxidized alpha-haemoglobin bound to AHSP reveals a protective mechanism for haem. Nature, 435(7042), 697-701. [More Information]
  • Mackay, J., Muiznieks, L., Toonkool, P., Weiss, A. (2005). The hydrophobic domain 26 of human tropoelastin is unstructured in solution. Journal of Structural Biology, 150(2), 154-162. [More Information]
  • Liew, C., Simpson, R., Kwan, A., Crofts, L., Loughlin, F., Matthews, J., Crossley, P., Mackay, J. (2005). Zinc fingers as protein recognition motifs: Structural basis for the GATA-1/ Friend of GATA interaction. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 102(3), 583-588. [More Information]
  • Simpson, R., Lee, S., Bartle, N., Sum, E., Visvader, J., Matthews, J., Mackay, J., Crossley, P. (2004). A Classic Zinc Finger From Friend Of Gata Mediates An Interaction With The Coiled-Coil Of Transforming Acidic Coiled-Coil 3. Journal of Biological Chemistry, 279(38), 39789-39797.
  • Donadini, R., Liew, C., Kwan, A., Mackay, J., Fields, B. (2004). Crystal And Solution Structures Of A Superantigen From Yersinia Pseudotuberculosis Reveal A Jelly-Roll Fold. Structure, 12(1), 145-156.
  • Kong, Y., Zhou, S., Kihm, A., Katein, A., Yu, X., Gell, D., Mackay, J., Adachi, K., Foster-Brown, L., Louden, C., et al (2004). Loss Of Alpha-Hemoglobin-Stabilizing Protein Impairs Erythropoiesis And Exacerbates Beta-Thalassemia. Journal of Clinical Investigation, 114(10), 1457-1466.
  • Feng, L., Gell, D., Zhou, S., Gu, L., Kong, Y., Li, J., Hu, M., Yan, N., Lee, C., Rich, A., Armstrong, R., Lay, P., Mackay, J., et al (2004). Molecular Mechanism Of AHSP-Mediated Stabilization Of A-Hemoglobin. Cell, 119(5), 629-640.
  • Dastmalchi, S., Church, W., Morris, M., Iismaa, T., Mackay, J. (2004). Presence of transient helical segments in the galanin-like peptide evident from 1H NMR, circular dichroism, and prediction studies. Journal of Structural Biology, 146(3), 261-271.
  • Chow, M., Mackay, J., Whisstock, J., Scanlon, M., Bottomley, S. (2004). Structural And Functional Analysis Of The Josephin Domain Of The Polyglutamine Protein Ataxin-3. Biochemical and Biophysical Research Communications, 322(2), 387-394. [More Information]
  • Westman, B., Perdomo, J., Matthews, J., Crossley, P., Mackay, J. (2004). Structural Studies On A Protein-Binding Zinc-Finger Domain Of Eos Reveal Both Similarities And Differences To Classical Zinc Fingers. Biochemistry, 43(42), 13318-13327.
  • Sunde, M., McGrath, K., Young, L., Matthews, J., Chua, E., Mackay, J., Heather, A. (2004). Tc-1 Is A Novel Tumorigenic And Natively Disordered Protein Associated With Thyroid Cancer. Cancer Research, 64(8), 2766-2773.
  • Kook, H., Lepore, J., Gitler, A., Lu, M., Yung, W., Mackay, J., Zhou, R., Ferrari, V., Gruber, P., Epstein, J. (2003). Cardiac hypertrophy and histone deacetylase-dependant transcriptional repression mediated by the atypical homeodomain protein Hop. Journal of Clinical Investigation, 112(6), 863-871.
  • Simpson, R., Cram, E., Czolij, T., Matthews, J., Crossley, P., Mackay, J. (2003). CCHX zinc finger derivatives retain the ability to bind Zn(II) and mediate protein-DNA interactions. Journal of Biological Chemistry, 278(30), 28011-28018.
  • Kwan, A., Gell, D., Verger, A., Crossley, P., Matthews, J., Mackay, J. (2003). Engineering a protein scaffold from a PhD finger. Structure, 11(7), 803-813.
  • Kwan, A., Czolij, R., Mackay, J., Crossley, P. (2003). Pentaprobe: a comprehensive sequence for the one-step detection of DNA-binding activities. Nucleic Acids Research, 31(20), e124-1-e124-11.
  • Proudfoot, E., Mackay, J., Karuso, P. (2003). Solution stucture and behaviour of Δ-cis-α [Ru(R, R-picchxnMe2)(phi)]2+ by NMR spectroscopy and molecular modelling. Dalton Transactions: an international journal of inorganic, organometallic and bioinorganic chemistry, 2003 (2), 165-170.
  • Deane, J., Mackay, J., Kwan, A., Sum, E., Visvader, J., Matthews, J. (2003). Structural basis for the recognition of Idb1 by the N-terminal LIM domains of LM02 and LM04. The EMBO Journal, 22(9), 2224-2233.
  • Westman, B., Perdomo, J., Sunde, M., Crossley, P., Mackay, J. (2003). The C-terminal domain fo Eos forms a high order complex in solution. Journal of Biological Chemistry, 278(43), 42419-42426.
  • Plambeck, C., Kwan, A., Adams, D., Westman, B., Van Der Weyden, L., Medcalf, R., Morris, B., Mackay, J. (2003). The structure of the Zinc finger domain from human splicing factor ZNF265 fold. Journal of Biological Chemistry, 278(25), 22805-22811.
  • Sharpe, B., Matthews, J., Kwan, A., Newton, A., Gell, D., Crossley, P., Mackay, J. (2002). A new zinc binding fold underlines the versatility of zinc binding modules in protein evolution. Structure, 10(5), 639-648.
  • Gell, D., Kong, Y., Eaton, S., Weiss, M., Mackay, J. (2002). Biophysical characterization of the a-globin binding protein a-hemoglobin stabilizing protein. Journal of Biological Chemistry, 277(43), 40602-40609.
  • Kowalski, K., Liew, C., Matthews, J., Gell, D., Crossley, P., Mackay, J. (2002). Characterization of the conserved interaction between GATA and FOG family proteins. Journal of Biological Chemistry, 277(38), 35720-35729.
  • Chen, F., Kook, H., Milewski, R., Gitler, A., Lu, M., Li, J., Nazarian, R., Schnepp, R., Jen, K., Biben, C., Mackay, J., et al (2002). Hop is an unusual homeobox gene that modulates cardiac development. Cell, 110(6), 713-723.
  • Westman, B., Mackay, J., Gell, D. (2002). Ikaros: a key regulator of haematopoiesis. The International Journal of Biochemistry and Cell Biology, , 1304-1307.
  • Deane, J., Visvader, J., Mackay, J., Matthews, J. (2002). Letter to the Editor: 1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex. Journal of Biomolecular NMR, 23(2), 165-166.
  • Polekhina, G., House, C., Traficante, N., Mackay, J., Relaix, F., Sassoon, D., Parker, M., Bowtell, D. (2002). Siah ubiquitin ligase structurally related to TRAF and modulates TNF-a signaling. Nature Structural and Molecular Biology, 9(1), 68-75.
  • Liepinsh, E., Otting, G., Harding, M., Ward, L., Mackay, J., Haymet, A. (2002). Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein. The FEBS Journal, 269(4), 1259-1266.
  • Robson, S., Michie, K., Mackay, J., Harry, E., King, G. (2002). The Bacillus subtilis cell division proteins FtsL and DivlC are intrinsically unstable and do not interact with one another in the absence of other septasomal components. Molecular Microbiology, 44(3), 663-674.
  • Pursglove, S., Mulhern, T., Mackay, J., Hinds, M., Booker, G. (2002). The solution structure and intramolecular associations of the Tec kinase Src homology 3 domain. Journal of Biological Chemistry, 277(1), 755-762.
  • Fairley, K., Westman, B., Pham, L., Haymet, A., Harding, M., Mackay, J. (2002). Type I Shorthorn Sculpin Antifreeze protein - Recombinant Synthesis, Solution Conformation, and Ice Growth Inhibition Studies. Journal of Biological Chemistry, 277(27), 24073-24080.
  • Deane, J., Sum, E., Mackay, J., Lindeman, G., Visvader, J., Matthews, J. (2001). Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular ldb1: LMO complexes. Protein Engineering, 14, 493-499.
  • Wang, X., Connor, M., Wilson, D., Wilson, H., Nicholson,, G., Smith, R., Shaw, D., Mackay, J., Alewood, P., Christie, M., et al (2001). Discovery and structure of a potent and highly specific blocker of insect calcium channels. Journal of Biological Chemistry, 276, 40306-40312.
  • Matthews, J., Visvader, J., Mackay, J. (2001). Letter to the Editor: 1H, 15N and 13C assignments of FLIN2, an intramolecular LMO2:ldb1 complex. Journal of Biomolecular NMR, 21(4), 385-386.
  • Proudfoot, E., Mackay, J., Karuso, P. (2001). Probing site specicity of DNA binding metallointercalators by NMR spectroscopy and molecular modeling. Biochemistry, 40, 4867-4878.
  • Mackay, J., Matthews, J., Winefield, R., Mackay, L., Haverkamp, R., Templeton, M. (2001). The hydrophobin EAS is largely unstructured in solution and functions by forming amyloid-like structures. Structure, 9, 83-91.
  • Newton, A., Mackay, J., Crossley, P. (2001). The N-terminal zinc finger of the erythroid transcription factor GATA-1 binds GATC motifs in DNA. Journal of Biological Chemistry, 276, 35794-35801.

Patents

  • Christopherson, R., Mackay, J., Matthews, J. (2012). Therapeutic Agents. Patent No. 5102772.

Reference Works

  • Grant, R., Crossley, P., Mackay, J. (2007). Zinc-finger Genes. John Wiley & Sons.

2014

  • Silva, A., Kwan, A., Mackay, J. (2014). 1H, 13C and 15N resonance assignments of an N-terminal domain of CHD4. Biomolecular NMR Assignments, 8(1), 137-139. [More Information]
  • Joseph, S., Kwan, A., Mackay, J., Cubeddu, L., Matthews, J. (2014). Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1. Biomolecular NMR Assignments, 8(1), 141-144. [More Information]
  • Alqarni, S., Murthy, A., Zhanh, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 sub-complex. The Journal of Biological Chemistry, 11 June 2014, 1-22. [More Information]
  • Alqarni, S., Murthy, A., Zhang, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 subcomplex. The Journal of Biological Chemistry, 289(32), 21844-21855. [More Information]
  • Dickson, C., Kumar, K., Jacques, D., Malmirchegini, G., Spirig, T., Mackay, J., Clubb, R., Guss, M., Gell, D. (2014). Structure of the hemoglobin-IsdH complex reveals the molecular basis of iron capture by Staphylococcus aureus. The Journal of Biological Chemistry, 289(10), 6728-6738. [More Information]
  • Mackay, J. (2014). Transcription factor seeks DNA - cognate site preferred. Journal of Molecular Biology, 426(7), 1370-1372. [More Information]

2013

  • Gamsjaeger, R., O'Connell, M., Cubeddu, L., Shepherd, N., Lowry, J., Kwan, A., Vandevenne, M., Swanton, M., Matthews, J., Mackay, J. (2013). A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers. Journal of Biological Chemistry, 288(49), 35180-35191. [More Information]
  • Dickson, C., Rich, A., D'Avigdor, W., Collins, D., Lowry, J., Mollan, T., Khandros, E., Olson, J., Weiss, M., Mackay, J., Lay, P., et al (2013). Alpha-hemoglobin-stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-alpha-hemoglobin and weakens the iron-oxygen bond. Journal of Biological Chemistry, 288(27), 19986-20001. [More Information]
  • Campbell, A., Wilkinson-White, L., Mackay, J., Matthews, J., Blobel, G. (2013). Analysis of disease-causing GATA1 mutations in murine gene complementation systems. Blood, 121(26), 5218-5227. [More Information]
  • Cordina, N., Liew, C., Gell, D., Fajer, P., Mackay, J., Brown, L. (2013). Effects of calcium binding and the hypertrophic cardiomyopathy A8V mutation on the dynamic equilibrium between closed and open conformations of the regulatory N-domain of isolated cardiac troponin C. Biochemistry, 52(11), 1950-1962. [More Information]
  • Mackay, J., Segal, D., Crossley, M. (2013). Is there a telltale RH fingerprint in zinc fingers that recognizes methylated CpG dinucleotides? Trends in Biochemical Sciences, 38(9), 421-422. [More Information]
  • Vandevenne, M., Jacques, D., Artuz, C., Nguyen, C., Kwan, A., Segal, D., Matthews, J., Crossley, M., Guss, M., Mackay, J. (2013). New insights into DNA recognition by zinc fingers revealed by structural analysis of the oncoprotein ZNF217. Journal of Biological Chemistry, 288(15), 10616-10627. [More Information]
  • Gamsjaeger, R., Kariawasam, R., Bang, L., Touma, C., Nguyen, C., Matthews, J., Cubeddu, L., Mackay, J. (2013). Semiquantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in surface plasmon resonance competition experiments. Analytical Biochemistry, 440(2), 178-185. [More Information]
  • Stokes, P., Liew, C., Kwan, A., Foo, P., Barker, H., Djamirze, A., O'Reilly, V., Visvader, J., Mackay, J., Matthews, J. (2013). Structural basis of the interaction of the breast cancer Oncogene LMO4 with the tumour suppressor CtIP/RBBP8. Journal of Molecular Biology, 425(7), 1101-1110. [More Information]

2012

  • Liew, C., Kwan, A., Stokes, P., Mackay, J., Matthews, J. (2012). 1H, 15N and 13C assignments of an intramolecular LMO4-LIM1/CtIP complex. Biomolecular NMR Assignments, 6(1), 31-34. [More Information]
  • Bendak, K., Loughlin, F., Cheung, V., O'Connell, M., Crossley, M., Mackay, J. (2012). A rapid method for assessing the RNA-binding potential of a protein. Nucleic Acids Research, 40(14), 1-11. [More Information]
  • Morris, V., Kwan, A., Mackay, J., Sunde, M. (2012). Backbone and sidechain 1H, 13C and 15N chemical shift assignments of the hydrophobin DewA from Aspergillus nidulans. Biomolecular N M R Assignments, 8, 83-86. [More Information]
  • Musselman, C., Ramirez, J., Sims, J., Mansfield, R., Oliver, S., Denu, J., Mackay, J., Wade, P., Hagman, J., Kutateladze, T. (2012). Bivalent recognition of nucleosomes by the tandem PHD fingers of the CHD4 ATPase is required for CHD4-mediated repression. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(3), 787-792. [More Information]
  • Christopherson, R., Mackay, J., Matthews, J. (2012). Therapeutic Agents. Patent No. 5102772.
  • McKenzie, J., Duyvestyn, J., Smith, T., Bendak, K., Mackay, J., Cursons, R., Cook, G., Arcus, V. (2012). Determination of ribonuclease sequence-specificity using Pentaprobes and mass spectrometry. RNA, 18(6), 1267-1278. [More Information]
  • Cordina, N., Liew, C., Gell, D., Fajer, P., Mackay, J., Brown, L. (2012). Interdomain orientation of cardiac Troponin C characterized by paramagnetic relaxation enhancement NMR reveals a compact state. Protein Science, 21(9), 1376-1387. [More Information]
  • O'Connell, M., Vandevenne, M., Nguyen, C., Matthews, J., Gamsjaeger, R., Segal, D., Mackay, J. (2012). Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angewandte Chemie (International Edition), 51(22), 5371-5375. [More Information]
  • Macindoe, I., Kwan, A., Ren, Q., Morris, V., Yang, W., Mackay, J., Sunde, M. (2012). Self-assembly of functional, amphipathic amyloid monolayers by the fungal hydrophobin EAS. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 109(14), E804-E811. [More Information]
  • Dastmalchi, S., Wilkinson-White, L., Kwan, A., Gamsjaeger, R., Mackay, J., Matthews, J. (2012). Solution structure of a tethered Lmo2(LIM2)/Ldb1(LID) complex. Protein Science, 21(11), 1768-1774. [More Information]
  • Bhati, M., Lee, C., Gadd, M., Jeffries, C., Kwan, A., Whitten, A., Trewhella, J., Mackay, J., Matthews, J. (2012). Solution Structure of the LIM-Homeodomain Transcription Factor Complex Lhx3/Ldb1 and the Effects of a Pituitary Mutation on Key Lhx3 Interactions. PLoS One, 7(7), 1-9. [More Information]
  • Hsieh, Y., Wilkinson, B., O'Connell, M., Mackay, J., Matthews, J., Payne, R. (2012). Synthesis of the bacteriocin glycopeptide sublancin 168 and S-glycosylated variants. Organic Letters, 14(7), 1910-1913. [More Information]
  • Gell, D., Mackay, J., Grant, R. (2012). The detection and quantitation of protein oligomerization. In Jacqueline M Matthews (Eds.), Protein Dimerization and Oligomerization in Biology, (pp. 19-41). New York: Springer Science + Business Media.
  • Burdach, J., O'Connell, M., Mackay, J., Crossley, M. (2012). Two-timing zinc finger transcription factors liaising with RNA. Trends in Biochemical Sciences, 37(5), 199-205. [More Information]

2011

  • Lamonica, J., Deng, W., Kadauke, S., Campbell, A., Gamsjaeger, R., Wang, H., Cheng, Y., Billin, A., Hardison, R., Mackay, J., et al (2011). Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 108(22), E159-E168. [More Information]
  • Nguyen, C., Mansfield, R., Leung, W., Vaz, P., Loughlin, F., Grant, R., Mackay, J. (2011). Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA. Journal of Molecular Biology, 407(2), 273-283. [More Information]
  • Lejon, S., Thong, S., Murthy, A., Alqarni, S., Murzina, N., Blobel, G., Laue, E., Mackay, J. (2011). Insights into Association of the NuRD Complex with FOG-1 from the Crystal Structure of an RbAp48.FOG-1 Complex. Journal of Biological Chemistry, 286(2), 1196-1203. [More Information]
  • Kwan, A., Mobli, M., Gooley, P., King, G., Mackay, J. (2011). Macromolecular NMR spectroscopy for the non-spectroscopist. The FEBS Journal, 278(5), 687-703. [More Information]
  • Bieri, M., Kwan, A., Mobli, M., King, G., Mackay, J., Gooley, P. (2011). Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination. The FEBS Journal, 278(5), 704-715. [More Information]
  • Mansfield, R., Musselman, C., Kwan, A., Oliver, S., Garske, A., Davrazou, F., Denu, J., Kutateladze, T., Mackay, J. (2011). Plant Homeodomain (PHD) Fingers of CHD4 Are Histone H3-binding Modules with Preference for Unmodified H3K4 and Methylated H3K9. Journal of Biological Chemistry, 286(13), 11779-11791. [More Information]
  • Wissmueller, S., Font Sadurni, J., Liew, C., Cram, E., Schroeder, T., Turner, J., Crossley, P., Mackay, J., Matthews, J. (2011). Protein-protein interactions: Analysis of a false positive GST pulldown result. Proteins: Structure, Function, and Bioinformatics, 79(8), 2365-2371. [More Information]
  • Gamsjaeger, R., Webb, S., Lamonica, J., Billin, A., Blobel, G., Mackay, J. (2011). Structural Basis and Specificity of Acetylated Transcription Factor GATA1 Recognition by BET Family Bromodomain Protein Brd3. Molecular and Cellular Biology, 31(13), 2632-2640. [More Information]
  • Krishna Kumar, K., Jacques, D., Pishchany, G., Caradoc-Davies, T., Spirig, T., Malmirchegini, G., Langley, D., Dickson, C., Mackay, J., Clubb, R., Guss, M., et al (2011). Structural Basis for Hemoglobin Capture by Staphylococcus aureus Cell-surface Protein, IsdH. Journal of Biological Chemistry, 286(44), 38439-38447. [More Information]
  • Wilkinson-White, L., Gamsjaeger, R., Dastmalchi, S., Wienert, B., Stokes, P., Crossley, M., Mackay, J., Matthews, J. (2011). Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 108(35), 14443-14448. [More Information]
  • Nunez Martin, N., Clifton, M., Funnell, A., Crisbel, A., Hallal, S., Quinlan (nee Edwards), K., Font Sadurni, J., Vandevenne, M., Setiyaputra, S., Pearson, R., Mackay, J., Crossley, P. (2011). The Multi-zinc Finger Protein ZNF217 Contacts DNA through a Two-finger Domain. Journal of Biological Chemistry, 286(44), 38190-38201. [More Information]
  • Mackay, J., Font Sadurni, J., Segal, D. (2011). The prospects for designer single-stranded RNA-binding proteins. Nature Structural and Molecular Biology, 18(3), 256-261. [More Information]
  • Setiyaputra, S., Mackay, J., Patrick, W. (2011). The Structure of a Truncated Phosphoribosylanthranilate Isomerase Suggests a Unified Model for Evolution of the (βα)8 Barrel Fold. Journal of Molecular Biology, 408(2), 291-303. [More Information]

2010

  • Hynson, R., Kwan, A., Jacques, D., Mackay, J., Trewhella, J. (2010). (1)H, (13)C and (15)N backbone and side chain resonance assignments of the N-terminal domain of the histidine kinase inhibitor KipI from Bacillus subtilis. Biomolecular N M R Assignments, 4(2), 167-169. [More Information]
  • Wilkinson-White, L., Dastmalchi, S., Kwan, A., Ryan, D., Mackay, J., Matthews, J. (2010). (1)H, (15)N and (13)C assignments of an intramolecular Lmo2-LIM2/Ldb1-LID complex. Biomolecular N M R Assignments, 4(2), 203-206. [More Information]
  • Krishna Kumar, K., Dickson, C., Weiss, M., Mackay, J., Gell, D. (2010). AHSP (α-haemoglobin-stabilizing protein) stabilizes apo-α-haemoglobin in a partially folded state. Biochemical Journal, 432(2), 275-282. [More Information]
  • Font, J., Mackay, J. (2010). Beyond DNA: Zinc Finger Domains as RNA-Binding Modules. Methods in Molecular Biology, 649, 479-491. [More Information]
  • Schwalbe, M., Dutta, K., Libich, D., Venugopal, H., Claridge, J., Gell, D., Mackay, J., Edwards, P., Pascal, S. (2010). Two-state conformational equilibrium in the Par-4 leucine zipper domain. Proteins: Structure, Function, and Bioinformatics, 78(11), 2433-2449. [More Information]

2009

  • Gell, D., Feng, L., Zhou, S., Jeffrey, P., Bendak, K., Gow, A., Weiss, M., Shi, Y., Mackay, J. (2009). A cis-proline in alpha-hemoglobin stabilizing protein directs the structural reorganization of alpha-hemoglobin. Journal of Biological Chemistry, 284(43), 29462-29469. [More Information]
  • Talib, J., Beck, J., Urathamakul, T., Nguyen, C., Aldrich-Wright, J., Mackay, J., Ralph, S. (2009). A mass spectrometric investigation of the ability of metal complexes to modulate transcription factor activity. Chemical Communications, (37), 5546-5548. [More Information]
  • Musselman, C., Mansfield, R., Garske, A., Davrazou, F., Kwan, A., Oliver, S., O'Leary, H., Denu, J., Mackay, J., Kutateladze, T. (2009). Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications. Biochemical Journal, 423(2), 179-187. [More Information]
  • Macindoe, I., Glockner, L., Vukasin, P., Stennard, F., Costa, M., Harvey, R., Mackay, J., Sunde, M. (2009). Conformational stability and DNA binding specificity of the cardiac T-box transcription factor Tbx20. Journal of Molecular Biology, 389(3), 606-618. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2009). Corrections. Biochemistry, 48(11), 2569-2570. [More Information]
  • Matthews, J., Bhati, M., Lehtomaki, E., Mansfield, R., Cubeddu, L., Mackay, J. (2009). It Takes Two to Tango: The Structure and Function of LIM, RING, PHD and MYND Domains. Current Pharmaceutical Design, 15(31), 3681-3969. [More Information]
  • Lowry, J., Gamsjaeger, R., Thong, S., Hung, W., Kwan, A., Broitman-Maduro, G., Matthews, J., Maduro, M., Mackay, J. (2009). Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains. Journal of Biological Chemistry, 284(9), 5827-5835. [More Information]
  • O'Connell, M., Gamsjaeger, R., Mackay, J. (2009). The structural analysis of protein-protein interactions by NMR spectroscopy. Proteomics - Clinical Applications, 9, 1-9. [More Information]
  • Loughlin, F., Mansfield, R., Vaz, P., McGrath, A., Setiyaputra, S., Gamsjaeger, R., Chen, E., Morris, B., Guss, M., Mackay, J. (2009). The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 106(14), 5581-5586. [More Information]

2008

  • Loughlin, F., Lee, M., Guss, M., Mackay, J. (2008). Crystallization of a ZRANB2-RNA complex. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(Pt 12), 1175-1177. [More Information]
  • Matthews, J., Loughlin, F., Mackay, J. (2008). Designed metal-binding sites in biomolecular and bioinorganic interactions. Current Opinion in Structural Biology, 18, 484-490. [More Information]
  • Wilkinson, S., Liew, C., Mackay, J., Salleh, H., Withers, S., McLeod, M. (2008). Escherichia coli glucuronylsynthase: An engineered enzyme for the synthesis of beta-glucuronides. Organic Letters, 10(8), 1585-1588. [More Information]
  • Wilkinson, S., Liew, C., Mackay, J., Salleh, H., Withers, S., McLeod, M. (2008). Escherichia coli Glucuronylsynthase: An Engineered Enzyme for the Synthesis of B-Glucuronides. Organic Letters, 10(8), 1585-1588. [More Information]
  • Griffin, M., Dobson, R., Pearce, F., Antonio, L., Whitten, A., Liew, C., Mackay, J., Trewhella, J., Jameson, G., Perugini, M., et al (2008). Evolution of quaternary structure in a homotetrameric enzyme. Journal of Molecular Biology, 380(4), 691-703. [More Information]
  • Bhati, M., Lee, C., Nancarrow, A., Lee, M., Craig, V., Bach, I., Guss, M., Mackay, J., Matthews, J. (2008). Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes. The EMBO Journal, 27, 2018-2029. [More Information]
  • Liew, C., Gamsjaeger, R., Mansfield, R., Mackay, J. (2008). NMR spectroscopy as a tool for the rapid assessment of the conformation of GST-fusion proteins. Protein Science, 17(9), 1630-1635. [More Information]
  • Mackay, J., Lowry, J., Crossley, P., Matthews, J. (2008). Response to Chatr-aryamontri et al.: Protein interactions: to believe or not to believe? Trends in Biochemical Sciences, 33. [More Information]
  • Gamsjaeger, R., Swanton, M., Kobus, F., Lehtomaki, E., Lowry, J., Kwan, A., Matthews, J., Mackay, J. (2008). Structural and biophysical analysis of the DNA-binding properties of myelin transcription factor 1. Journal of Biological Chemistry, 283(8), 5158-5167. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2008). Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum. Biochemistry, 47(12), 3842-3854. [More Information]
  • Kwan, A., Macindoe, I., Vukasin, P., Morris, V., Kass, I., Gupte, R., Mark, A., Templeton, M., Mackay, J., Sunde, M. (2008). The Cys3-Cys4 Loop of the Hydrophobin EAS Is Not Required for Rodlet Formation and Surface Activity. Journal of Molecular Biology, 382, 708-720. [More Information]

2007

  • Yu, X., Kong, Y., Dore, L., Abdulmalik, O., Katein, A., Zhou, S., Choi, J., Gell, D., Mackay, J., Gow, A., et al (2007). An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. Journal of Clinical Investigation, 117(7), 1856-1865. [More Information]
  • Wang, C., Guncar, G., Forwood, J., Teh, T., Catanzariti, A., Lawrence, G., Loughlin, F., Mackay, J., Schirra, H., Anderson, P., et al (2007). Crystal Structures of Flax Rust Avirulence Proteins AvrL567-A and -D Reveal Details of the Structural Basis for Flax Disease Resistance Specificity. Plant Cell, 19(9), 2898-2912. [More Information]
  • Porter, C., Matthews, J., Mackay, J., Pursglove, S., Schmidberger, J., Leedman, P., Pero, S., Krag, D., Wilce, M., Wilce, J. (2007). Grb7 SH2 domain structure and interactions with a cyclic peptide inhibitor of cancer cell migration and proliferation. B M C Structural Biology, 7(Article number 58), 1-15.
  • Kirk, E., Sunde, M., Costa, M., Rankin, S., Wolstein, O., Castro, M., Butler, T., Hyun, C., Guo, G., Otway, R., Mackay, J., Sholler, G., Winlaw, D., et al (2007). Mutations in Cardiac T-Box Factor Gene TBX20 Are Associated with Diverse Cardiac Pathologies, Including Defects of Septation and Valvulogenesis and Cardiomyopathy. American Journal of Human Genetics, 81(2), 280-291. [More Information]
  • Mackay, J., Sunde, M., Lowry, J., Crossley, P., Matthews, J. (2007). Protein interactions: is seeing believing? Trends in Biochemical Sciences, 32(12), 530-531. [More Information]
  • Gamsjaeger, R., Liew, C., Loughlin, F., Crossley, P., Mackay, J. (2007). Sticky fingers: zinc-fingers as protein-recognition motifs. Trends in Biochemical Sciences, 32(2), 63-70. [More Information]
  • Sunde, M., Kwan, A., Templeton, M., Beever, R., Mackay, J. (2007). Structural analysis of hydrophobins. Micron, doi:10.1016/j.micron.2007.08.003, 1-12. [More Information]
  • Grant, R., Crossley, P., Mackay, J. (2007). Zinc-finger Genes. John Wiley & Sons.

2006

  • Gell, D., Westman, B., Gorman, D., Liew, C., Liew, J., Weiss, M., Mackay, J. (2006). A Novel Haem-binding Interface in the 22 kDa Haem-binding Protein p22HBP. Journal of Molecular Biology, 362(2), 287-297. [More Information]
  • Kook, H., Yung, W., Simpson, R., Kee, H., Shin, S., Lowry, J., Loughlin, F., Yin, Z., Epstein, J., Mackay, J. (2006). Analysis of the Structure and Function of the Transcriptional Coregulator HOP. Biochemistry, 45(35), 10584-10590. [More Information]
  • Lowry, J., Mackay, J. (2006). GATA-1: one protein, many partners. The International Journal of Biochemistry and Cell Biology, 38(1), 6-11. [More Information]
  • Ryan, D., Sunde, M., Kwan, A., Marianayagam, N., Nancarrow, A., vanden Hoven, R., Thompson, L., Baca, M., Mackay, J., Visvader, J., Matthews, J. (2006). Identification of the key LMO2-binding determinants on Ldb1. Journal of Molecular Biology. [More Information]
  • Liew, C., Rand, K., Simpson, R., Yung, W., Mansfield, R., Crossley, P., Proetorius-Ibba, M., Nerlov, C., Poulsen, F., Mackay, J. (2006). Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1. Journal of Biological Chemistry, 281(38), 28296-28306. [More Information]
  • Liew, C., Crossley, P., Mackay, J., Nicholas, H. (2006). Solution Structure of the THAP Domain from Caenorhabditis elegans C-terminal Binding Protein (CtBP). Journal of Molecular Biology, 366(2), 382-390. [More Information]
  • Kwan, A., Winefield, R., Sunde, M., Matthews, J., Haverkamp, R., Templeton, M., Mackay, J. (2006). Structural basis for rodlet assembly in fungal hydrophobins. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 103(10), 3621-3626. [More Information]
  • Loughlin, F., Mackay, J. (2006). Zinc fingers are known as domains for binding DNA and RNA. Do they also mediate protein-protein interactions? IUBMB Life, 58(12), 731-733. [More Information]

2005

  • Lee, C., Nancarrow, A., Bach, I., Mackay, J., Matthews, J. (2005). 1H, 15N and 13C assignments of an intramolecular Lhx3:ldb1 complex. Journal of Biomolecular NMR, 33(3), 198-198. [More Information]
  • Duggin, I., Matthews, J., Dixon, N., Wake, R., Mackay, J. (2005). A complex mechanism determines polarity of DNA replication fork arrest by the replication terminator complex of Bacillus subtilis. Journal of Biological Chemistry, 280(13), 13105-13113. [More Information]
  • Sharpe, B., Liew, C., Kwan, A., Wilce, J., Crossley, P., Matthews, J., Mackay, J. (2005). Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting. Structure, 13(2), 257-266. [More Information]
  • Pursglove, S., Mackay, J. (2005). CSL: A notch above the rest. The International Journal of Biochemistry and Cell Biology, 37(12), 2472-2477. [More Information]
  • Porter, C., Wilce, M., Mackay, J., Leedman, P., Wilce, J. (2005). Grb7-SH2 domain dimerisation is affected by a single point mutation. European Biophysics Journal, 34(5), 454-460. [More Information]
  • Weiss, M., Zhou, S., Feng, L., Gell, D., Mackay, J., Shi, Y., Gow, A. (2005). Role of Alpha Hemoglobin-Stabilizing Protein in Normal Erythropoiesis and {beta}-Thalassemia. New York Academy of Sciences. Annals, 1054, 103-117. [More Information]
  • Kwan, A., Fairley, K., Anderberg, P., Liew, C., Harding, M., Mackay, J. (2005). Solution structure of a recombinant type I sculpin antifreeze protein. Biochemistry, 44(6), 1980-1988. [More Information]
  • Feng, L., Zhou, S., Gu, L., Gell, D., Mackay, J., Weiss, M., Gow, A., Shi, Y. (2005). Structure of oxidized alpha-haemoglobin bound to AHSP reveals a protective mechanism for haem. Nature, 435(7042), 697-701. [More Information]
  • Mackay, J., Muiznieks, L., Toonkool, P., Weiss, A. (2005). The hydrophobic domain 26 of human tropoelastin is unstructured in solution. Journal of Structural Biology, 150(2), 154-162. [More Information]
  • Liew, C., Simpson, R., Kwan, A., Crofts, L., Loughlin, F., Matthews, J., Crossley, P., Mackay, J. (2005). Zinc fingers as protein recognition motifs: Structural basis for the GATA-1/ Friend of GATA interaction. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 102(3), 583-588. [More Information]

2004

  • Simpson, R., Lee, S., Bartle, N., Sum, E., Visvader, J., Matthews, J., Mackay, J., Crossley, P. (2004). A Classic Zinc Finger From Friend Of Gata Mediates An Interaction With The Coiled-Coil Of Transforming Acidic Coiled-Coil 3. Journal of Biological Chemistry, 279(38), 39789-39797.
  • Donadini, R., Liew, C., Kwan, A., Mackay, J., Fields, B. (2004). Crystal And Solution Structures Of A Superantigen From Yersinia Pseudotuberculosis Reveal A Jelly-Roll Fold. Structure, 12(1), 145-156.
  • Kong, Y., Zhou, S., Kihm, A., Katein, A., Yu, X., Gell, D., Mackay, J., Adachi, K., Foster-Brown, L., Louden, C., et al (2004). Loss Of Alpha-Hemoglobin-Stabilizing Protein Impairs Erythropoiesis And Exacerbates Beta-Thalassemia. Journal of Clinical Investigation, 114(10), 1457-1466.
  • Feng, L., Gell, D., Zhou, S., Gu, L., Kong, Y., Li, J., Hu, M., Yan, N., Lee, C., Rich, A., Armstrong, R., Lay, P., Mackay, J., et al (2004). Molecular Mechanism Of AHSP-Mediated Stabilization Of A-Hemoglobin. Cell, 119(5), 629-640.
  • Dastmalchi, S., Church, W., Morris, M., Iismaa, T., Mackay, J. (2004). Presence of transient helical segments in the galanin-like peptide evident from 1H NMR, circular dichroism, and prediction studies. Journal of Structural Biology, 146(3), 261-271.
  • Chow, M., Mackay, J., Whisstock, J., Scanlon, M., Bottomley, S. (2004). Structural And Functional Analysis Of The Josephin Domain Of The Polyglutamine Protein Ataxin-3. Biochemical and Biophysical Research Communications, 322(2), 387-394. [More Information]
  • Westman, B., Perdomo, J., Matthews, J., Crossley, P., Mackay, J. (2004). Structural Studies On A Protein-Binding Zinc-Finger Domain Of Eos Reveal Both Similarities And Differences To Classical Zinc Fingers. Biochemistry, 43(42), 13318-13327.
  • Sunde, M., McGrath, K., Young, L., Matthews, J., Chua, E., Mackay, J., Heather, A. (2004). Tc-1 Is A Novel Tumorigenic And Natively Disordered Protein Associated With Thyroid Cancer. Cancer Research, 64(8), 2766-2773.

2003

  • Kook, H., Lepore, J., Gitler, A., Lu, M., Yung, W., Mackay, J., Zhou, R., Ferrari, V., Gruber, P., Epstein, J. (2003). Cardiac hypertrophy and histone deacetylase-dependant transcriptional repression mediated by the atypical homeodomain protein Hop. Journal of Clinical Investigation, 112(6), 863-871.
  • Simpson, R., Cram, E., Czolij, T., Matthews, J., Crossley, P., Mackay, J. (2003). CCHX zinc finger derivatives retain the ability to bind Zn(II) and mediate protein-DNA interactions. Journal of Biological Chemistry, 278(30), 28011-28018.
  • Kwan, A., Gell, D., Verger, A., Crossley, P., Matthews, J., Mackay, J. (2003). Engineering a protein scaffold from a PhD finger. Structure, 11(7), 803-813.
  • Kwan, A., Czolij, R., Mackay, J., Crossley, P. (2003). Pentaprobe: a comprehensive sequence for the one-step detection of DNA-binding activities. Nucleic Acids Research, 31(20), e124-1-e124-11.
  • Proudfoot, E., Mackay, J., Karuso, P. (2003). Solution stucture and behaviour of Δ-cis-α [Ru(R, R-picchxnMe2)(phi)]2+ by NMR spectroscopy and molecular modelling. Dalton Transactions: an international journal of inorganic, organometallic and bioinorganic chemistry, 2003 (2), 165-170.
  • Deane, J., Mackay, J., Kwan, A., Sum, E., Visvader, J., Matthews, J. (2003). Structural basis for the recognition of Idb1 by the N-terminal LIM domains of LM02 and LM04. The EMBO Journal, 22(9), 2224-2233.
  • Westman, B., Perdomo, J., Sunde, M., Crossley, P., Mackay, J. (2003). The C-terminal domain fo Eos forms a high order complex in solution. Journal of Biological Chemistry, 278(43), 42419-42426.
  • Plambeck, C., Kwan, A., Adams, D., Westman, B., Van Der Weyden, L., Medcalf, R., Morris, B., Mackay, J. (2003). The structure of the Zinc finger domain from human splicing factor ZNF265 fold. Journal of Biological Chemistry, 278(25), 22805-22811.

2002

  • Sharpe, B., Matthews, J., Kwan, A., Newton, A., Gell, D., Crossley, P., Mackay, J. (2002). A new zinc binding fold underlines the versatility of zinc binding modules in protein evolution. Structure, 10(5), 639-648.
  • Gell, D., Kong, Y., Eaton, S., Weiss, M., Mackay, J. (2002). Biophysical characterization of the a-globin binding protein a-hemoglobin stabilizing protein. Journal of Biological Chemistry, 277(43), 40602-40609.
  • Kowalski, K., Liew, C., Matthews, J., Gell, D., Crossley, P., Mackay, J. (2002). Characterization of the conserved interaction between GATA and FOG family proteins. Journal of Biological Chemistry, 277(38), 35720-35729.
  • Chen, F., Kook, H., Milewski, R., Gitler, A., Lu, M., Li, J., Nazarian, R., Schnepp, R., Jen, K., Biben, C., Mackay, J., et al (2002). Hop is an unusual homeobox gene that modulates cardiac development. Cell, 110(6), 713-723.
  • Westman, B., Mackay, J., Gell, D. (2002). Ikaros: a key regulator of haematopoiesis. The International Journal of Biochemistry and Cell Biology, , 1304-1307.
  • Deane, J., Visvader, J., Mackay, J., Matthews, J. (2002). Letter to the Editor: 1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex. Journal of Biomolecular NMR, 23(2), 165-166.
  • Polekhina, G., House, C., Traficante, N., Mackay, J., Relaix, F., Sassoon, D., Parker, M., Bowtell, D. (2002). Siah ubiquitin ligase structurally related to TRAF and modulates TNF-a signaling. Nature Structural and Molecular Biology, 9(1), 68-75.
  • Liepinsh, E., Otting, G., Harding, M., Ward, L., Mackay, J., Haymet, A. (2002). Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein. The FEBS Journal, 269(4), 1259-1266.
  • Robson, S., Michie, K., Mackay, J., Harry, E., King, G. (2002). The Bacillus subtilis cell division proteins FtsL and DivlC are intrinsically unstable and do not interact with one another in the absence of other septasomal components. Molecular Microbiology, 44(3), 663-674.
  • Pursglove, S., Mulhern, T., Mackay, J., Hinds, M., Booker, G. (2002). The solution structure and intramolecular associations of the Tec kinase Src homology 3 domain. Journal of Biological Chemistry, 277(1), 755-762.
  • Fairley, K., Westman, B., Pham, L., Haymet, A., Harding, M., Mackay, J. (2002). Type I Shorthorn Sculpin Antifreeze protein - Recombinant Synthesis, Solution Conformation, and Ice Growth Inhibition Studies. Journal of Biological Chemistry, 277(27), 24073-24080.

2001

  • Deane, J., Sum, E., Mackay, J., Lindeman, G., Visvader, J., Matthews, J. (2001). Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular ldb1: LMO complexes. Protein Engineering, 14, 493-499.
  • Wang, X., Connor, M., Wilson, D., Wilson, H., Nicholson,, G., Smith, R., Shaw, D., Mackay, J., Alewood, P., Christie, M., et al (2001). Discovery and structure of a potent and highly specific blocker of insect calcium channels. Journal of Biological Chemistry, 276, 40306-40312.
  • Matthews, J., Visvader, J., Mackay, J. (2001). Letter to the Editor: 1H, 15N and 13C assignments of FLIN2, an intramolecular LMO2:ldb1 complex. Journal of Biomolecular NMR, 21(4), 385-386.
  • Proudfoot, E., Mackay, J., Karuso, P. (2001). Probing site specicity of DNA binding metallointercalators by NMR spectroscopy and molecular modeling. Biochemistry, 40, 4867-4878.
  • Mackay, J., Matthews, J., Winefield, R., Mackay, L., Haverkamp, R., Templeton, M. (2001). The hydrophobin EAS is largely unstructured in solution and functions by forming amyloid-like structures. Structure, 9, 83-91.
  • Newton, A., Mackay, J., Crossley, P. (2001). The N-terminal zinc finger of the erythroid transcription factor GATA-1 binds GATC motifs in DNA. Journal of Biological Chemistry, 276, 35794-35801.

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