Dr Mario Torrado Del Rey

Postdoctoral Research Associate
School of Life and Environmental Sciences

G08 - Biochemistry and Microbiology Building
The University of Sydney

Telephone +61 2 9351 3746
Website Mackay lab website

Biographical details

Dr. Mario Torrado del Rey is currently working at Prof. Joel Mackay's laboratory at the University of Sydney. He joined the lab for his first postdoctoral experience in 2014, to work in protein structure, function and interactions. In this regard, he is especially focused on the improvement of the protein production stage.

Before that, Mario completed his Bachelor Degree in Biochemistry (2002) and his Ph.D. in Molecular Biology (2012) at the Autonoma University of Madrid, Spain. His Ph.D. work studied the stability-function relationship in homeodomains, by structural and thermodynamic approaches such as circular dichroism, isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR). This work was done under the supervision of Dr. Juan Luis Asensio at the Institute of Organic Chemistry, CSIC, Madrid, Spain.

Research interests

Mario’s interests in protein structure, function and interactions are fulfilled by his work in three main projects:

- Structural and functional study of chromatin remodelers. Mario is focused on trying to produce recombinant versions of these in mammalian and insect cells for structural and functional studies, including single particle electron microscopy. He is also interested in delineating the network of pairwise interactions between different subunits of these complexes, mainly by immunoprecipitation analyses.

- High throughput screening of binding and functional properties of transcription factors. For this purpose, multiple transcription factors are expressed and purified at the same time, and then spotted on microarrays. Experiments performed then on the microarrays allow for many different questions to be asked of the transcription factors.

- Structural study of the proteins involved in the enucleation of red blood cells. The approach involves the combination of different nuclear magnetic resonance (NMR) and X-ray crystallography experiments, amongst others.

Teaching and supervision

Mario helps co-supervise PhD, Honours, TSP, and summer students, as well as visiting international students in Prof. Joel Mackay’s laboratory. He has also experience in demonstrating in 1st and 2nd year undergraduate labs.

In the media

Google Scholar.

Links to download Mario’s Ph.D. thesis:

Source 1

Source 2

Selected publications

Download citations: PDF RTF Endnote

Journals

  • Torrado Del Rey, M., Low, J., Silva, A., Schmidberger, J., Sana, M., Sharifi Tabar, M., Isilak, M., Winning, C., Kwong, C., Bedward, M., Mackay, J., et al (2017). Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex. The FEBS Journal, In Press, 1-17. [More Information]
  • Low, J., Webb, S., Silva, A., Saathoff, H., Ryan, D., Torrado Del Rey, M., Brofelth, M., Parker, B., Shepherd, N., Mackay, J. (2016). CHD4 Is a Peripheral Component of the Nucleosome Remodeling and Deacetylase Complex. Journal of Biological Chemistry, 291(30), 15853-15866. [More Information]
  • Schmidberger, J., Sharifi Tabar, M., Torrado Del Rey, M., Silva, A., Landsberg, M., Brillault, L., Alqarni, S., Zeng, Y., Parker, B., Low, J., Mackay, J. (2016). The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/7. Protein Science, 25, 1472-1482. [More Information]
  • Torrado, M., Revuelta, J., Gonzalez, C., Corzana, F., Bastida, A., Asensio, J. (2009). Role of Conserved Salt Bridges in Homeodomain Stability and DNA Binding. Journal of Biological Chemistry, 284(35), 23765-23779. [More Information]

2017

  • Torrado Del Rey, M., Low, J., Silva, A., Schmidberger, J., Sana, M., Sharifi Tabar, M., Isilak, M., Winning, C., Kwong, C., Bedward, M., Mackay, J., et al (2017). Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex. The FEBS Journal, In Press, 1-17. [More Information]

2016

  • Low, J., Webb, S., Silva, A., Saathoff, H., Ryan, D., Torrado Del Rey, M., Brofelth, M., Parker, B., Shepherd, N., Mackay, J. (2016). CHD4 Is a Peripheral Component of the Nucleosome Remodeling and Deacetylase Complex. Journal of Biological Chemistry, 291(30), 15853-15866. [More Information]
  • Schmidberger, J., Sharifi Tabar, M., Torrado Del Rey, M., Silva, A., Landsberg, M., Brillault, L., Alqarni, S., Zeng, Y., Parker, B., Low, J., Mackay, J. (2016). The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/7. Protein Science, 25, 1472-1482. [More Information]

2009

  • Torrado, M., Revuelta, J., Gonzalez, C., Corzana, F., Bastida, A., Asensio, J. (2009). Role of Conserved Salt Bridges in Homeodomain Stability and DNA Binding. Journal of Biological Chemistry, 284(35), 23765-23779. [More Information]

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