Dr Nicholas Shepherd

ARC DECRA Fellow

G08 - Biochemistry and Microbiology Building
The University of Sydney

Telephone +61 2 9351 3911

Research interests

My research is broadly focused on developing chemical tools to study biological systems. I am interested in the way molecules interact at a molecular level and how this relates to their biological function. We are developing synthetic molecules to study, mimic and perturb transcriptional processes in vitro and in vivo.

Keywords

Proteomics; Cell biology; Biochemistry; Drug design

Selected grants

2014

  • University of Sydney Automated Peptide Synthesis Platform; Payne R, Britton W, Byrne S, Chan H, Clarke R, Hunt N, Jolliffe K, Kassiou M, Lay P, Mackay J, Matthews J, McErlean C, New E, Rasmussen H, Rendina L, Rudrawar S, Rutledge P, Shepherd N; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Structural and functional analysis of a cancer-linked co-regulator complex; Mackay J, Shepherd N, Ryan D; National Health and Medical Research Council (NHMRC)/Project Grants.

2013

  • Multiangle laser light scattering instrument for protein characterisation; Mackay J, Ataide S, Jormakka M, Ryan R, Matthews J, Shepherd N, Sunde M, Collyer C, Payne R; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • HPLC-MS for high throughput drug and protein analysis; Payne R, Kassiou M, Cordwell S, Hibbs D, Groundwater P, Shepherd N; DVC Research/Equipment Grant.

2012

  • Innovative chemical tools for the isolation, biochemical and structural analysis of biological macromolecular assemblies; Shepherd N; Australian Research Council (ARC)/Discovery Early Career Researcher Award (DECRA).

Selected publications

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Journals

  • Vandevenne, M., O'Connell, M., Helder, S., Shepherd, N., Matthews, J., Kwan, A., Segal, D., Mackay, J. (2014). Engineering Specificity Changes on a RanBP2 Zinc Finger that Binds Single-Stranded RNA. Angewandte Chemie (International Edition), 53(30), 7848-7852. [More Information]
  • Alqarni, S., Murthy, A., Zhanh, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 sub-complex. The Journal of Biological Chemistry, 11 June 2014, 1-22. [More Information]
  • Alqarni, S., Murthy, A., Zhang, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 subcomplex. The Journal of Biological Chemistry, 289(32), 21844-21855. [More Information]
  • Parker, B., Shepherd, N., Trefely, S., Hoffman, N., White, M., Engholm-Keller, K., Hambly, B., Larsen, M., James, D., Cordwell, S. (2014). Structural Basis for Phosphorylation and Lysine Acetylation Crosstalk in a Kinase Motif Associated with Myocardial Ischemia and Cardioprotection. The Journal of Biological Chemistry, 289(37), 25890-25906. [More Information]
  • Clifton, M., Westman, B., Thong, S., O'Connell, M., Webster, M., Shepherd, N., Quinlan (nee Edwards), K., Crossley, P., Blobel, G., Mackay, J. (2014). The Identification and Structure of an N-Terminal PR Domain Show that FOG1 Is a Member of the PRDM Family of Proteins. PloS One, 9(8), e106011. [More Information]
  • Gamsjaeger, R., O'Connell, M., Cubeddu, L., Shepherd, N., Lowry, J., Kwan, A., Vandevenne, M., Swanton, M., Matthews, J., Mackay, J. (2013). A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers. Journal of Biological Chemistry, 288(49), 35180-35191. [More Information]
  • Shepherd, N., Harrison, R., Fairlie, D. (2012). Targeting quorum sensing and competence stimulation for antimicrobial chemotherapy. Current Drug Targets (online), 13(11), 1348-1359. [More Information]
  • Shepherd, N., Tanabe, H., Xu, Y., Matsunaga, S., Shibasaki, M. (2010). Direct catalytic asymmetric vinylogous mannich-type and michael reactions of an alpha,beta-unsaturated gamma-butyrolactam under dinuclear nickel catalysis. Journal of the American Chemical Society, 132(11), 3666-3667. [More Information]
  • Harrison, R., Shepherd, N., Hoang, H., Ruiz-Gomez, G., Hill, T., Driver, R., Desai, V., Young, P., Abbenante, G., Fairlie, D. (2010). Downsizing human, bacterial, and viral proteins to short water-stable alpha helices that maintain biological potency. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 107(26), 11686-11691. [More Information]
  • Harrison, R., Ruiz-Gomez, G., Hill, T., Chow, S., Shepherd, N., Lohman, R., Abbenante, G., Hoang, H., Fairlie, D. (2010). Novel helix-constrained nociceptin derivatives are potent agonists and antagonists of ERK phosphorylation and thermal analgesia in mice. Journal of Medicinal Chemistry, 53(23), 8400-8408. [More Information]
  • Mihara, H., Shepherd, N., Matsunaga, S., Shibasaki, M. (2009). A heterobimetallic Ga/Yb-Schiff base complex for catalytic asymmetric alpha-addition of isocyanides to aldehydes. Journal of the American Chemical Society, 131(24), 8384-8385. [More Information]
  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2009). Left- and right-handed alpha-helical turns in homo- and hetero-chiral helical scaffolds. Journal of the American Chemical Society, 131(43), 15877-15886. [More Information]
  • Shepherd, N., Hoang, H., Desai, V., Letouze, E., Young, P., Fairlie, D. (2006). Modular α-helical mimetics with antiviral activity against respiratory syncitial virus. Journal of the American Chemical Society, 128(40), 13284-13289. [More Information]
  • Baell, J., Duggan, P., Forsyth, S., Lewis, R., Lok, Y., Schroeder, C., Shepherd, N. (2006). Synthesis and biological evaluation of anthranilamide-based non-peptide mimetics of u-conotoxin GVIA. Tetrahedron, 62(31), 7284-7292. [More Information]
  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2005). Single Turn Peptide Alpha Helices with Exceptional Stability in Water. Journal of the American Chemical Society, 127(9), 2974-2983. [More Information]

Conferences

  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2005). Generating Short Stable α-Helices with Cyclic Pentapeptides. International Chemical Congress of Pacific Basin Societies PacifiChem 2005, United States: American Chemical Society. [More Information]

2014

  • Vandevenne, M., O'Connell, M., Helder, S., Shepherd, N., Matthews, J., Kwan, A., Segal, D., Mackay, J. (2014). Engineering Specificity Changes on a RanBP2 Zinc Finger that Binds Single-Stranded RNA. Angewandte Chemie (International Edition), 53(30), 7848-7852. [More Information]
  • Alqarni, S., Murthy, A., Zhanh, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 sub-complex. The Journal of Biological Chemistry, 11 June 2014, 1-22. [More Information]
  • Alqarni, S., Murthy, A., Zhang, W., Przewloka, M., Silva, A., Watson, A., Lejon, S., Pei, X., Smits, A., Kloet, S., Shepherd, N., Stokes, P., Mackay, J., et al (2014). Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 subcomplex. The Journal of Biological Chemistry, 289(32), 21844-21855. [More Information]
  • Parker, B., Shepherd, N., Trefely, S., Hoffman, N., White, M., Engholm-Keller, K., Hambly, B., Larsen, M., James, D., Cordwell, S. (2014). Structural Basis for Phosphorylation and Lysine Acetylation Crosstalk in a Kinase Motif Associated with Myocardial Ischemia and Cardioprotection. The Journal of Biological Chemistry, 289(37), 25890-25906. [More Information]
  • Clifton, M., Westman, B., Thong, S., O'Connell, M., Webster, M., Shepherd, N., Quinlan (nee Edwards), K., Crossley, P., Blobel, G., Mackay, J. (2014). The Identification and Structure of an N-Terminal PR Domain Show that FOG1 Is a Member of the PRDM Family of Proteins. PloS One, 9(8), e106011. [More Information]

2013

  • Gamsjaeger, R., O'Connell, M., Cubeddu, L., Shepherd, N., Lowry, J., Kwan, A., Vandevenne, M., Swanton, M., Matthews, J., Mackay, J. (2013). A Structural Analysis of DNA Binding by Myelin Transcription Factor 1 Double Zinc Fingers. Journal of Biological Chemistry, 288(49), 35180-35191. [More Information]

2012

  • Shepherd, N., Harrison, R., Fairlie, D. (2012). Targeting quorum sensing and competence stimulation for antimicrobial chemotherapy. Current Drug Targets (online), 13(11), 1348-1359. [More Information]

2010

  • Shepherd, N., Tanabe, H., Xu, Y., Matsunaga, S., Shibasaki, M. (2010). Direct catalytic asymmetric vinylogous mannich-type and michael reactions of an alpha,beta-unsaturated gamma-butyrolactam under dinuclear nickel catalysis. Journal of the American Chemical Society, 132(11), 3666-3667. [More Information]
  • Harrison, R., Shepherd, N., Hoang, H., Ruiz-Gomez, G., Hill, T., Driver, R., Desai, V., Young, P., Abbenante, G., Fairlie, D. (2010). Downsizing human, bacterial, and viral proteins to short water-stable alpha helices that maintain biological potency. Proceedings of the National Academy of Sciences (PNAS) of the United States of America, 107(26), 11686-11691. [More Information]
  • Harrison, R., Ruiz-Gomez, G., Hill, T., Chow, S., Shepherd, N., Lohman, R., Abbenante, G., Hoang, H., Fairlie, D. (2010). Novel helix-constrained nociceptin derivatives are potent agonists and antagonists of ERK phosphorylation and thermal analgesia in mice. Journal of Medicinal Chemistry, 53(23), 8400-8408. [More Information]

2009

  • Mihara, H., Shepherd, N., Matsunaga, S., Shibasaki, M. (2009). A heterobimetallic Ga/Yb-Schiff base complex for catalytic asymmetric alpha-addition of isocyanides to aldehydes. Journal of the American Chemical Society, 131(24), 8384-8385. [More Information]
  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2009). Left- and right-handed alpha-helical turns in homo- and hetero-chiral helical scaffolds. Journal of the American Chemical Society, 131(43), 15877-15886. [More Information]

2006

  • Shepherd, N., Hoang, H., Desai, V., Letouze, E., Young, P., Fairlie, D. (2006). Modular α-helical mimetics with antiviral activity against respiratory syncitial virus. Journal of the American Chemical Society, 128(40), 13284-13289. [More Information]
  • Baell, J., Duggan, P., Forsyth, S., Lewis, R., Lok, Y., Schroeder, C., Shepherd, N. (2006). Synthesis and biological evaluation of anthranilamide-based non-peptide mimetics of u-conotoxin GVIA. Tetrahedron, 62(31), 7284-7292. [More Information]

2005

  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2005). Generating Short Stable α-Helices with Cyclic Pentapeptides. International Chemical Congress of Pacific Basin Societies PacifiChem 2005, United States: American Chemical Society. [More Information]
  • Shepherd, N., Hoang, H., Abbenante, G., Fairlie, D. (2005). Single Turn Peptide Alpha Helices with Exceptional Stability in Water. Journal of the American Chemical Society, 127(9), 2974-2983. [More Information]

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