Professor Richard Christopherson

Professor

G08 - Biochemistry and Microbiology Building
The University of Sydney

Telephone +61 2 9351 6031
Fax +61 2 9351 4726

Website Related website

Research interests

Fludarabine induces proteins that regulate apoptosis in leukaemia cells

The purine analogues, cladribine and fludarabine, are effective as single agents in treating chronic lymphocytic leukaemia (CLL) and hairy cell leukaemia (HCL). The mechanisms of action of these anticancer drugs against CLL and HCL are being investigated using CD antibody (DotScan) microarrays and two-dimensional fluorescence differential gel electrophoresis (DIGE). We have found that both drugs induce significant changes in surface expression profiles on CLL cells, and in levels of several proteins involved in apoptosis. Hsp90 inhibitors reduce oncogenic signalling proteins in leukaemia cells Heat shock protein 90 (Hsp90) inhibitors such as 17-AAG are undergoing clinical trials for treatment of a variety of cancers. Hsp90 is a molecular chaperone that catalyzes the conformational maturation of a number of oncogenic signalling proteins with the hydrolysis of ATP. Inhibitors such as 17-AAG prevent the binding of ATP to Hsp90, resulting in the release and degradation of signalling proteins required for the growth of cancer. We have found that several Hsp90 inhibitors induce significant changes in the surface expression profiles and cellular proteomes of CLL and other leukaemias. The results obtained will provide insight on the mechanisms of action of these novel drugs. Classification of leukaemias by cell surface profiling DotScan antibody microarrays capture live leukaemia cells providing an extensive immunophenotype (surface expression profile or disease signature) for that leukaemia. This novel technology has been validated for classification of leukaemias by a clinical trial involving 796 patients. DotScan has also been used to identify significant changes induced in the immunophenotype of the human myeloid cell line, HL60, induced by all-trans retinoic acid (ATRA), Vitamin D and phorbol esters. Surface profiling of colorectal cancers and detection of tumour-infiltrating lymphocytes (TILs) DotScan has also been used to determine surface expression profiles of colorectal cancer (CRC) cells from surgically resected specimens. Using fluorescence multiplexing, expression profiles of CRC cells and tumour infiltrating lymphocytes (TILs) have been obtained from cancerous polyps. TILs may be very important in the future for cancer prognosis and for therapy that involves growing these TILs in vitro and infusing them back into the patient for immunotherapy.

Keywords

Melanoma and skin cancer; Haematological malignancy; Proteomics; Biochemistry; Colorectal cancers

Selected grants

2014

  • CLARIOstar Multifunctional Microplate Reader for Shared Used at the Open Access, Multi-User Molecular Biology Core Facility; Huang M, Scolyer R, Fraser S, Sahni S, Speranza T, Owens T, Barrs V, Ju Y, Kovacevic Z, Lu Z, Camp A, Slobedman B, Morris B, Oliver B, Dos Remedios C, Kalinowski D, Fu D, Lai D, Lovicu F, Allbutt H, Triccas J, Burgess J, Kril J, Dixon K, Rendina L, Byrne M, King N, Groundwater P, Williamson P, Ho J, Dong Q, Bao B, Assinder S, McLennan S, Richardson D, Ammit A, Sharland A, Hardikar A, Hong A, Hambly B, Lee C, Murphy C, Goldsbury C, Johnstone D, Lane D, Hibbs D, Joshua D, Huq F, Halliday G, Sutherland G, Zreiqat H, Lok H, Lyons G, Jansson P, Black J, Zhu L, Sunde M, Day M, Naylor M, Buckland M, Murray M, Shafie N, Lay P, Poronnik P, Codd R, Mason R, Ryan R, Christopherson R, de Graaf S; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2013

  • Robotic High Throughput Western Analysis for the Open Access, Multi-User Sydney Cancer Research Core Facility; Richardson D, Scolyer R, Boyer M, Halliday G, Damian D, Christopherson R, Joshua D, Kench J, Hong A, Murray M, Lee C, Kalinowski D, Naylor M, Lay P, Lyons G, Kovacevic Z, Mason R, Dixon K, Chan-Ling T, Hawkins C, Sunde M, Lovejoy D, Owens T, Rendina L, Jansson P, Dos Remedios C, Charles (nee Slaviero) K, Lane D, Witting P, Dong Q, Ammit A, Groundwater P, Assinder S, Bao B, Byrne S, Zhou F, Buckland M, Grewal T, Huq F, Lai D, Codd R, Zhang D, Fu D, de Graaf S, Huang M, Payne R, Slobedman B, Barrs V, Ho J, Williamson P, Murphy C; DVC Research/Equipment Grant.
  • Application of biological nuclide labelling to probe the transduction of vibrational energy of enzymes to chemical potential for catalysis; Christopherson R; Australian Institute of Nuclear Science and Engineering (AINSE)/Awards.

2012

  • CFUND: CDIP 1049 Heterodimeric demibodies as highly selective therapautics and diagnostics (2005-002); Christopherson R.

2011

  • Melanoma Institute Australia Translational Research Program; Mann G, Thompson J, Kefford R, Menzies S, Scolyer R, Christopherson R, Rizos H, Spillane A, Zhang X, Hersey P; Cancer Institute New South Wales/Translational Program Grant.
  • Patterns of differentially abundant proteins on progressive chronic lymphocytic leukaemias; Christopherson R, Mulligan S; Leukaemia Foundation of Australia/Grants in Aid.

2010

  • A mass spectrometer for multiple reaction monitoring to validate cancer biomarkers; Christopherson R; Cancer Institute New South Wales/Equipment Grant.
  • Next-generation high-speed flow cytometric cell sorter; Fazekas B, King N, Simpson S, Britton W, Campbell I, Vadas M, Grau G, Weninger W, Christopherson R, Gamble J, Rasko J, McCaughan G, Xia P, Beale P, Holst J, Haass N, Combes V, Saunders B, Byrne S, West N, Gorrell M, Bertolino P, Bowen D, Warner F, Seth D, Grimshaw M, Jolly C, Martiniello-Wilks R, Mrass P, Lyons G, Sword G; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2009

  • 7-laser BD LSR-II and Cellomics Array Scan VTi to Enhance Capability and Throughput for the NSW Advanced Cytometry Facility; King N, Weninger W, Gunning P, Fazekas B, Campbell I, Christopherson R, Black J, Mason R, Richardson D, Halliday G, Grau G, Gandevia S, Kachigian L, Geczy C, Jessup W, Vadas M, Britton W, Hunt N; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).
  • Script translational grant project; Christopherson R; New South Wales Health/Research Support.
  • Core Resource Facility - application for 7-laser flow cytometer and high content bioimaging system to enhance capabilities and throughput for the NSW Advanced Cytometry Facility; King N, Vadas M, Campbell I, Grau G, Britton W, Fazekas B, Richardson D, Halliday G, Christopherson R, Gamble J, Mason R, Rasko J, McCaughan G, Weninger W, Xia P, Gorrell M, Holst J, Pile A, Sword G, McArthur C, Dong Q, Haass N, Sharland A, Saunders B, Triccas J, Norris J, Bertolino P, Bowen D, Sze D; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2008

  • Proteomic responses of B-lymphoproliferative disorders to fludarabine; Christopherson R, Mulligan S; Leukaemia Foundation of Australia/Grants in Aid.
  • Nanotherapeutics: nanoparticles with high specificity for the delivery and controlled release of drugs; Masters A, Maschmeyer T, Christopherson R; Australian Research Council (ARC)/Discovery Projects (DP).
  • CLLARC- Proteomic changes induced in Chronic Lymphocytic Leukaemia by Fludaranine; Christopherson R; Leukaemia Foundation of NSW/Research Grant Program.
  • Application of CD Antibody Microarray Tehcnology to Study the Effects of MAP kinase and HSP90 Inhibitors on Leukemia Cells; Graves M, Christopherson R, Graves M; University of Sydney/International Visiting Research Fellowship (IVRF).

2007

  • Use of Proteomic Analyses to improve the management of colorectal cancer - the SCRIPT for CRC Projec; Chan C, Kohonen-Corish M, Robertson G, Clarke S, Baker M, Beale P, Christopherson R, Bokey E, Horvath L, Lin B, Hong A, Solomon M, Chapuis P, McKay M, Mark M; Cancer Institute New South Wales/Translational Program Grant.
  • Proteomic screening for apoptotic markers in cancer; Baxter R, Leong S, Christopherson R; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Funding the Proteomics Facility 2007-09; Christopherson R; University of Sydney/Internal Research Grant Allocations.

2006

  • Sydney Melanoma Unit Translational Research Program; Mann G, Thompson J, Kefford R, Scolyer R, Menzies S, Hersey P, Christopherson R; Cancer Institute New South Wales/Translational Research Program Grant.
  • Global phenotype profiling of chronic lymphocytic leukemia; Mulligan S, Christopherson R; CLL (Chronic Lymphocytic Leukemia) Global Research Foundation/Project Grant.
  • High resolution bioanalytical Fourier transform mass spectrometer combined with liquid chromatograph; Christopherson R, Baker M, Raftery M, Geczy C, James D, Harry E, Guilhaus M, Cavicchioli R, Stocker R, Wilkinson M, Dos Remedios C, Davey R; Australian Research Council (ARC)/Linkage Infrastructure, Equipment and Facilities (LIEF).

2005

  • FACS Aria for the University of Sydney Flow Cytometry Facility; King N, Pollard J, Rasko J, Lovicu F, Bishop A, Bertolino P, Briscoe H, Gillies M, Madigan M, McAvoy J, Murphy C, Balcar V, Armour C, Hughes M, Reeve V, Christopherson R, Halliday G, Christie M, Pollock C, McCaughan G, Gorrell M, Dong Q, Abendroth A, Menzies S, Barnetson R, Byrne S, Hunt N, Hambly B, Cook D, Mason R, Day M, Black J; University of Sydney/Equipment Grant.
  • ProteomeX MS and personnel support for the new facuility; Baxter R, Christopherson R, Daly R, Guilhaus M, Kavallaris M, Clarke S, Molloy M, Baker M; Cancer Institute New South Wales/Research Support.

2004

  • Proteomic analysis of subcellular changes during apoptosis; Baxter R, Christopherson R; Australian Research Council (ARC)/Discovery Projects (DP).

2003

  • Recombinant malarial pyrimidine enzymes as drug targets; Christopherson R, Radajewski S; National Health and Medical Research Council (NHMRC)/Project Grants.
  • SELDI Protein Chip System; Baxter R, Christopherson R, King N; University of Sydney/Major Equipment.

2002

  • Recombinant malarial pyrimidine enzymes as drug targets; Christopherson R; DVC Research/Bridging Support Grant.
  • Diagnosis of leukaemias by CD antigen expression; Chrisp J, Garret N, Christopherson R; Medsaic Pty Ltd/Research Support.
  • RC L3022 - Diagnosis of leukaemias by CD antigen expression & Protocol No X02-0056 & RC K0301- Inflammation and CD antigen expression: cardiopulmonary bypass v OPCAB surgery; Dos Remedios C, Christopherson R; Medsaic Pty Ltd/BLO Project.

2001

  • A recombinant protein facility at the University of Sydney; Christopherson R, Wake R, Basten A, Britton W; WELLCOME TRUST/Major Equipment Awards for Biomedical Research in Australia.

2000

  • Comparative determination of the three-dimensional structures of prokaryotic and mammalian dihydroorotases; Christopherson R; Australian Research Council (ARC)/Small Grants.

Selected publications

Download citations: PDF RTF Endnote

Book Chapters

  • Zhou, J., Belov, L., Armstrong, N., Christopherson, R. (2013). Antibody Microarrays and Multiplexing. In Xiangdong Wang (Eds.), Bioinformatics of Human Proteomics, (pp. 331-359). Berlin: Springer.
  • Christopherson, R. (2006). Nucleotide Biosynthesis. In Ganten, Detlev; Ruckpaul, Klaus (Eds.), Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine, (pp. 1333-1337). NY United States: Springer.

Journals

  • Chik, J., Zhou, J., Moh, E., Christopherson, R., Clarke, S., Molloy, M., Packer, N. (2014). Comprehensive glycomics comparison between colon cancer cell cultures and tumours: Implications for biomarker studies. International Journal of Proteomics, 108(C), 146-162. [More Information]
  • Cooper, M., Cox, N., Zimmerman, E., Dewar, B., Duncan, J., Whittle, M., Nguyen, T., Jones, L., Roy, S., Smalley, D., Christopherson, R., et al (2013). Application of Multiplexed Kinase Inhibitor Beads to Study Kinome Adaptations in Drug-Resistant Leukemia. PLoS One, 8(6), 1-14. [More Information]
  • Che, Y., Best, G., Zhong, L., Kaufman (nee Alexander), K., Mactier, S., Raftery, M., Graves, L., Mulligan, S., Christopherson, R. (2013). Hsp90 Inhibitor SNX-7081 Dysregulates Proteins Involved with DNA Repair and Replication and the Cell Cycle in Human Chronic Lymphocytic Leukemia (CLL) Cells. Journal of Proteome Research, 12(4), 1710-1722. [More Information]
  • Huang, P., Kohnke, P., Belov, L., Best, G., Mulligan, S., Christopherson, R. (2013). Profiles of surface mosaics on chronic lymphocytic leukemias distinguish stable and progressive subtypes. Journal of Pharmacy and Pharmaceutical Sciences, 16(2), 231-237. [More Information]
  • Rahman, W., Huang, P., Belov, L., Chrisp, J., Christopherson, R., Stapelberg, P., Warner, F., George, J., Bowen, D., Strasser, S., Koorey, D., Sharland, A., McCaughan, G., Shackel, N. (2012). Analysis of human liver disease using a cluster of differentiation (CD) antibody microarray. Liver International, 32(10), 1527-1534. [More Information]
  • Leong, S., McKay, M., Christopherson, R., Baxter, R. (2012). Biomarkers of Breast Cancer Apoptosis Induced by Chemotherapy and TRAIL. Journal of Proteome Research, 11(2), 1240-1250. [More Information]
  • Kohnke, P., Mactier, S., Almazi, J., Crossett, B., Christopherson, R. (2012). Fludarabine and Cladribine Induce Changes in Surface Proteins on Human B-Lymphoid Cell Lines Involved with Apoptosis, Cell Survival, and Antitumor Immunity. Journal of Proteome Research. [More Information]
  • Almazi, J., Mactier, S., Best, G., Crossett, B., Mulligan, S., Christopherson, R. (2012). Fludarabine nucleoside induces accumulations of p53, p63 and p73 in the nuclei of human B-lymphoid cell lines, with cytosolic and mitochondrial increases in p53. Proteomics - Clinical Applications, 6(5-6), 279-290. [More Information]
  • Leong, S., Nunez, A., Lin, Z., Crossett, B., Christopherson, R., Baxter, R. (2012). iTRAQ-Based Proteomic Profiling of Breast Cancer Cell Response to Doxorubicin and TRAIL. Journal of Proteome Research, 11(7), 3561-3572. [More Information]
  • Huang, P., Best, G., Belov, L., Mulligan, S., Christopherson, R. (2012). Surface profiles for subclassification of chronic lymphocytic leukemia. Leukemia and Lymphoma, 53(6), 1046-1056. [More Information]
  • Best, G., Che, Y., Singh, N., Forsyth, C., Christopherson, R., Mulligan, S. (2012). The Hsp90 inhibitor SNX-7081 synergizes with and restores sensitivity to fludarabine in chronic lymphocytic leukemia cells with lesions in the TP53 pathway: a potential treatment strategy for fludarabine refractory disease. Leukemia and Lymphoma, 53(7), 1367-1375. [More Information]
  • Mallawaaratchy, D., Mactier, S., Kaufman (nee Alexander), K., Blomfield, S., Christopherson, R. (2012). The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. Journal of Proteomics, 75(5), 1590-1599. [More Information]
  • Heath, E., Kaufman (nee Alexander), K., Christopherson, R. (2011). B-RAF: A Contributor to the Melanoma Phenotype. The International Journal of Biochemistry and Cell Biology, 43(1), 29-32. [More Information]
  • Belov, L., Zhou, J., Christopherson, R. (2011). Cell Surface Markers in Colorectal Cancer Prognosis. International Journal of Molecular Sciences, 12(1), 78-113. [More Information]
  • Zhou, J., Belov, L., Solomon, M., Chan, C., Clarke, S., Christopherson, R. (2011). Colorectal cancer cell surface protein profiling using an antibody microarray and fluorescence multiplexing. Journal of Visualized Experiments, (55), 1-6. [More Information]
  • Mactier, S., Henrich, S., Che, Y., Kohnke, P., Christopherson, R. (2011). Comprehensive proteomic analysis of the effects of purine analogs on human Raji B-cell lymphoma. Journal of Proteome Research, 10(3), 1030-1042. [More Information]
  • Henrich, S., Mactier, S., Best, G., Mulligan, S., Crossett, B., Christopherson, R. (2011). Fludarabine Nucleoside Modulates Nuclear "Survival and Death" Proteins in Resistant Chronic Lymphocytic Leukemia Cells. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 30(12), 1181-1189. [More Information]
  • Kaufman (nee Alexander), K., Belov, L., Huang, P., Mactier, S., Scolyer, R., Mann, G., Christopherson, R. (2010). An extended antibody microarray for surface profiling metastatic melanoma. Journal of Immunological Methods, 358(40210 (1-2)), 23-34. [More Information]
  • Cassano, C., Mactier, S., Mulligan, S., Belov, L., Huang, P., Christopherson, R. (2010). Cladribine and Fludarabine Nucleoside Change the Levels of CD Antigens on B-Lymphoproliferative Disorders. International Journal of Proteomics, 2010 (Article ID 964251), 964251-1-964251-9. [More Information]
  • Yang, F., Tay, K., Dong, L., Thorne, R., Jiang, C., Yang, E., Tseng, H., Liu, H., Christopherson, R., Hersey, P., et al (2010). Cystatin B inhibition of TRAIL-induced apoptosis is associated with the protection of FLIP(L) from degradation by the E3 ligase itch in human melanoma cells. Cell Death and Differentiation, 17(8), 1354-1367. [More Information]
  • Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, E., Chan, C., Clarke, C., Clarke, S., Christopherson, R. (2010). Surface antigen profiling of colorectal cancer using antibody microarrays with fluorescence multiplexing. Journal of Immunological Methods, 355(40210 (1-2)), 40-51. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2009). Corrections. Biochemistry, 48(11), 2569-2570. [More Information]
  • Kohnke, P., Mulligan, S., Christopherson, R. (2009). Membrane proteomics for leukemia classification and drug target identification. Current Opinion in Molecular Therapeutics, 11(6), 603-610. [More Information]
  • Barber, N., Gez, S., Belov, L., Mulligan, S., Woolfson, A., Christopherson, R. (2009). Profiling CD antigens on leukaemias with an antibody microarray. FEBS Letters, 583(11), 1785-1791. [More Information]
  • Barber, N., Belov, L., Christopherson, R. (2008). All-trans retinoic acid induces different immunophenotypic changes on human HL60 and NB4 myeloid leukaemias. Leukemia Research: clinical and laboratory studies, 32, 315-322. [More Information]
  • Henrich, S., Mactier, S., Crossett, B., Mulligan, S., Christopherson, R. (2008). Fludarabine induces differential expression of proteins in human leukemia and lymphoma cells. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 27, 634-640. [More Information]
  • Henrich, S., Christopherson, R. (2008). Multiple forms of nuclear p53 formed in human Raji and MEC1 cells treated with fludarabine. Leukemia, 22(3), 657-660. [More Information]
  • Ellmark, P., Hogerkorp, C., Ek, S., Belov, L., Berglund, M., Rosenquist, R., Christopherson, R., Borrebaeck, C. (2008). Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays. Cancer Letters, 265(1), 98-106. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2008). Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum. Biochemistry, 47(12), 3842-3854. [More Information]
  • Mactier, S., Crossett, B., Christopherson, R. (2007). Differentially expressed cytosolic proteins in human leukemia and lymphoma cell lines correlate with lineages and functions. Biochimica et Biophysica Acta. Proteins and Proteomics, 1774 (9), 1173-1183. [More Information]
  • Henrich, S., Crossett, B., Christopherson, R. (2007). Differentially expressed nuclear proteins in human CCRF-CEM, HL-60, MECA and Raji cells correlate with cellular properties. Proteomics - Clinical Applications, 1(10), 1252-1265. [More Information]
  • Lee, M., Maher, M., Christopherson, R., Guss, M. (2007). Kinetic and Structural Analysis of Mutant Escherichia coli Dihydroorotases: A Flexible Loop Stabilizes the Transition State(,). Biochemistry, 46(37), 10538-10550. [More Information]
  • Leong, S., Christopherson, R., Baxter, R. (2007). Profiling of apoptotic changes in human breast cancer cells using SELDI-TOF mass spectrometry. Cellular Physiology and Biochemistry, 20(5), 579-590. [More Information]
  • Lee, M., Chan, C., Graham, S., Christopherson, R., Guss, M., Maher, M. (2007). Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: Implications for loop movement in inhibitor design. Journal of Molecular Biology, 370(5), 812-825. [More Information]
  • Ellmark, P., Woolfson, A., Belov, L., Christopherson, R. (2007). The applicability of a cluster of differentiation monoclonal antibody microarray to the diagnosis of human disease. Methods in Molecular Biology, 439(14). [More Information]
  • Henrich, S., Cordwell, S., Crossett, B., Baker, M., Christopherson, R. (2007). The nuclear proteome and DNA-binding fraction of human Raji lymphoma cells. Biochimica et Biophysica Acta. Proteins and Proteomics, 1774 (4), 413-432. [More Information]
  • Anderson, M., Cleland, W., Huang, D., Chan, C., Shojaei, M., Christopherson, R. (2006). 13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus. Biochemistry, 45(23), 7132-7139. [More Information]
  • Belov, L., Mulligan, S., Barber, N., Woolfson, A., Scott, M., Stoner, K., Chrisp, J., Sewell, W., Bradstock, K., Bendall, L., Sartor, M., Young, G., Wiley, J., Christopherson, R., et al (2006). Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray. British Journal of Haematology, 135(2), 184-97. [More Information]
  • Christopherson, R., Stoner, K., Barber, N., Belov, L., Woolfson, A., Scott, M., Bendall, L., Mulligan, S. (2006). Classification of AML using a monoclonal antibody microarray. Methods in molecular medicine, 125, 241-251. [More Information]
  • Ellmark, P., Belov, L., Huang, P., Lee, C., Solomon, M., Morgan, D., Christopherson, R. (2006). Multiplex detection of surface molecules on colorectal cancers. Proteomics, 6(6), 1791-1802. [More Information]
  • Woolfson, A., Ellmark, P., Chrisp, J., Scott, M., Christopherson, R. (2006). The application of CD antigen proteomics to pharmacogenomics. Pharmacogenomics, 7(5), 759-771. [More Information]
  • Huang, D., Kaplan, J., Menz, R., Katis, V., Wake, R., Zhao, F., Wolfenden, R., Christopherson, R. (2006). Thermodynamic Analysis of Catalysis by the Dihydroorotases from Hamster and Bacillus caldolyticus, As Compared with the Uncatalyzed Reaction. Biochemistry, 45(27), 8275-8283. [More Information]
  • Woolfson, A., Stebbing, J., Tom, B., Stoner, K., Gilks, W., Kreil, D., Mulligan, S., Belov, L., Chrisp, J., Errington, W., Christopherson, R., et al (2005). Conservation of unique cell-surface CD antigen mosaics in HIV-1-infected individuals. Blood, 106(3), 1003-1007. [More Information]
  • Lee, M., Chan, C., Guss, M., Christopherson, R., Maher, M. (2005). Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits. Journal of Molecular Biology, 348(3), 523-533. [More Information]
  • Georgiou, J., Skarratt, K., Fuller, S., Martin, C., Christopherson, R., Wiley, J., Sluyter, R. (2005). Human Epidermal and Monocyte-Derived Langerhans Cells Express Functional P2X7 Receptors. The Journal of Investigative Dermatology, 125(3), 482-490. [More Information]
  • White, S., Belov, L., Barber, N., Hodgkin, P., Christopherson, R. (2005). Immunophenotypic changes induced on human HL60 leukaemia cells by 1alpha,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate. Leukemia Research: clinical and laboratory studies, 29(10), 1141-1151. [More Information]
  • Belov, L., Huang, P., Chrisp, J., Mulligan, S., Christopherson, R. (2005). Screening microarrays of novel monoclonal antibodies for binding to T-, B- and myeloid leukaemia cells. Journal of Immunological Methods, 305(1), 10-19. [More Information]
  • Kamal, M., Christopherson, R. (2004). Accumulation Of 5-Phosphoribosyl-1-Pyrophosphate In Human CCRF-CEM Leukaemia Cells Treated With Antifolates. The International Journal of Biochemistry and Cell Biology, 36(3), 545-551. [More Information]
  • Christopherson, R., Cinquin, O., Shojaei, M., Kuehn, D., Menz, R. (2004). Cloning and expression of malarial pyrimidine enzymes. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 23(8&9), 1459-1465.
  • Wilson, P., Mulligan, S., Christopherson, R. (2004). Metabolic Response Patterns Of Nucleotides In B-Cell Chronic Lymphocytic Leukaemias To Cladribine, Fludarabine And Deoxycoformycin. Leukemia Research: clinical and laboratory studies, 28(7), 725-731.
  • Maher, M., Huang, D., Guss, M., Collyer, C., Christopherson, R. (2003). Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form. Acta Crystallographica. Section D: Biological Crystallography, 59(2), 381-384.
  • Belov, L., Huang, P., Barber, N., Mulligan, S., Christopherson, R. (2003). Identification of repertoires of surface antigens on leukemias using an antibody mircroarray. Proteomics, 3(11), 2147-2154. [More Information]
  • Lal, S., Christopherson, R., Dos Remedios, C. (2002). Antibody arrays: an embryonic but rapidly growing technology. Drug Discovery Today, 7(18 Supplement), S143-S149.
  • Christopherson, R., Lyons, S., Wilson, P. (2002). Inhibitors of de Novo nucleotide biosynthesis as drugs. Accounts Of Chemical Research, 35(11), 961-971.
  • Menz, R., Cinquin, O., Christopherson, R. (2002). The identification, cloning and functional expression of the gene encoding orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum. Annals Of Tropical Medicine And Parasitology, 96(5), 469-476.
  • Christopherson, R., Menz, R., Cinquin, O. (2001). A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli. Molecular and Biochemical Parasitology, 117, 245-247.
  • Belov, L., de la Vega, O., Dos Remedios, C., Christopherson, R., Mulligan,, S. (2001). Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer Research, 61(11), 4483-4489.

Conferences

  • Sluyter, R., Georgiou, J., Skarratt, K., Fuller, S., Martin, C., Christopherson, R., Wiley, J. (2005). Human epidermal and monocyte-derived langerhans cells express functional P2X7 receptors. 35th Annual Scientific Meeting of the Australasian Society for Immunology 2005, Denmark: Blackwell Munksgaard.

Reference Works

  • Christopherson, R. (2008). CD antigens. In Schwab M (Eds.), Encyclopedia of Cancer. Springer.

2014

  • Chik, J., Zhou, J., Moh, E., Christopherson, R., Clarke, S., Molloy, M., Packer, N. (2014). Comprehensive glycomics comparison between colon cancer cell cultures and tumours: Implications for biomarker studies. International Journal of Proteomics, 108(C), 146-162. [More Information]

2013

  • Zhou, J., Belov, L., Armstrong, N., Christopherson, R. (2013). Antibody Microarrays and Multiplexing. In Xiangdong Wang (Eds.), Bioinformatics of Human Proteomics, (pp. 331-359). Berlin: Springer.
  • Cooper, M., Cox, N., Zimmerman, E., Dewar, B., Duncan, J., Whittle, M., Nguyen, T., Jones, L., Roy, S., Smalley, D., Christopherson, R., et al (2013). Application of Multiplexed Kinase Inhibitor Beads to Study Kinome Adaptations in Drug-Resistant Leukemia. PLoS One, 8(6), 1-14. [More Information]
  • Che, Y., Best, G., Zhong, L., Kaufman (nee Alexander), K., Mactier, S., Raftery, M., Graves, L., Mulligan, S., Christopherson, R. (2013). Hsp90 Inhibitor SNX-7081 Dysregulates Proteins Involved with DNA Repair and Replication and the Cell Cycle in Human Chronic Lymphocytic Leukemia (CLL) Cells. Journal of Proteome Research, 12(4), 1710-1722. [More Information]
  • Huang, P., Kohnke, P., Belov, L., Best, G., Mulligan, S., Christopherson, R. (2013). Profiles of surface mosaics on chronic lymphocytic leukemias distinguish stable and progressive subtypes. Journal of Pharmacy and Pharmaceutical Sciences, 16(2), 231-237. [More Information]

2012

  • Rahman, W., Huang, P., Belov, L., Chrisp, J., Christopherson, R., Stapelberg, P., Warner, F., George, J., Bowen, D., Strasser, S., Koorey, D., Sharland, A., McCaughan, G., Shackel, N. (2012). Analysis of human liver disease using a cluster of differentiation (CD) antibody microarray. Liver International, 32(10), 1527-1534. [More Information]
  • Leong, S., McKay, M., Christopherson, R., Baxter, R. (2012). Biomarkers of Breast Cancer Apoptosis Induced by Chemotherapy and TRAIL. Journal of Proteome Research, 11(2), 1240-1250. [More Information]
  • Kohnke, P., Mactier, S., Almazi, J., Crossett, B., Christopherson, R. (2012). Fludarabine and Cladribine Induce Changes in Surface Proteins on Human B-Lymphoid Cell Lines Involved with Apoptosis, Cell Survival, and Antitumor Immunity. Journal of Proteome Research. [More Information]
  • Almazi, J., Mactier, S., Best, G., Crossett, B., Mulligan, S., Christopherson, R. (2012). Fludarabine nucleoside induces accumulations of p53, p63 and p73 in the nuclei of human B-lymphoid cell lines, with cytosolic and mitochondrial increases in p53. Proteomics - Clinical Applications, 6(5-6), 279-290. [More Information]
  • Leong, S., Nunez, A., Lin, Z., Crossett, B., Christopherson, R., Baxter, R. (2012). iTRAQ-Based Proteomic Profiling of Breast Cancer Cell Response to Doxorubicin and TRAIL. Journal of Proteome Research, 11(7), 3561-3572. [More Information]
  • Huang, P., Best, G., Belov, L., Mulligan, S., Christopherson, R. (2012). Surface profiles for subclassification of chronic lymphocytic leukemia. Leukemia and Lymphoma, 53(6), 1046-1056. [More Information]
  • Best, G., Che, Y., Singh, N., Forsyth, C., Christopherson, R., Mulligan, S. (2012). The Hsp90 inhibitor SNX-7081 synergizes with and restores sensitivity to fludarabine in chronic lymphocytic leukemia cells with lesions in the TP53 pathway: a potential treatment strategy for fludarabine refractory disease. Leukemia and Lymphoma, 53(7), 1367-1375. [More Information]
  • Mallawaaratchy, D., Mactier, S., Kaufman (nee Alexander), K., Blomfield, S., Christopherson, R. (2012). The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. Journal of Proteomics, 75(5), 1590-1599. [More Information]

2011

  • Heath, E., Kaufman (nee Alexander), K., Christopherson, R. (2011). B-RAF: A Contributor to the Melanoma Phenotype. The International Journal of Biochemistry and Cell Biology, 43(1), 29-32. [More Information]
  • Belov, L., Zhou, J., Christopherson, R. (2011). Cell Surface Markers in Colorectal Cancer Prognosis. International Journal of Molecular Sciences, 12(1), 78-113. [More Information]
  • Zhou, J., Belov, L., Solomon, M., Chan, C., Clarke, S., Christopherson, R. (2011). Colorectal cancer cell surface protein profiling using an antibody microarray and fluorescence multiplexing. Journal of Visualized Experiments, (55), 1-6. [More Information]
  • Mactier, S., Henrich, S., Che, Y., Kohnke, P., Christopherson, R. (2011). Comprehensive proteomic analysis of the effects of purine analogs on human Raji B-cell lymphoma. Journal of Proteome Research, 10(3), 1030-1042. [More Information]
  • Henrich, S., Mactier, S., Best, G., Mulligan, S., Crossett, B., Christopherson, R. (2011). Fludarabine Nucleoside Modulates Nuclear "Survival and Death" Proteins in Resistant Chronic Lymphocytic Leukemia Cells. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 30(12), 1181-1189. [More Information]

2010

  • Kaufman (nee Alexander), K., Belov, L., Huang, P., Mactier, S., Scolyer, R., Mann, G., Christopherson, R. (2010). An extended antibody microarray for surface profiling metastatic melanoma. Journal of Immunological Methods, 358(40210 (1-2)), 23-34. [More Information]
  • Cassano, C., Mactier, S., Mulligan, S., Belov, L., Huang, P., Christopherson, R. (2010). Cladribine and Fludarabine Nucleoside Change the Levels of CD Antigens on B-Lymphoproliferative Disorders. International Journal of Proteomics, 2010 (Article ID 964251), 964251-1-964251-9. [More Information]
  • Yang, F., Tay, K., Dong, L., Thorne, R., Jiang, C., Yang, E., Tseng, H., Liu, H., Christopherson, R., Hersey, P., et al (2010). Cystatin B inhibition of TRAIL-induced apoptosis is associated with the protection of FLIP(L) from degradation by the E3 ligase itch in human melanoma cells. Cell Death and Differentiation, 17(8), 1354-1367. [More Information]
  • Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, E., Chan, C., Clarke, C., Clarke, S., Christopherson, R. (2010). Surface antigen profiling of colorectal cancer using antibody microarrays with fluorescence multiplexing. Journal of Immunological Methods, 355(40210 (1-2)), 40-51. [More Information]

2009

  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2009). Corrections. Biochemistry, 48(11), 2569-2570. [More Information]
  • Kohnke, P., Mulligan, S., Christopherson, R. (2009). Membrane proteomics for leukemia classification and drug target identification. Current Opinion in Molecular Therapeutics, 11(6), 603-610. [More Information]
  • Barber, N., Gez, S., Belov, L., Mulligan, S., Woolfson, A., Christopherson, R. (2009). Profiling CD antigens on leukaemias with an antibody microarray. FEBS Letters, 583(11), 1785-1791. [More Information]

2008

  • Barber, N., Belov, L., Christopherson, R. (2008). All-trans retinoic acid induces different immunophenotypic changes on human HL60 and NB4 myeloid leukaemias. Leukemia Research: clinical and laboratory studies, 32, 315-322. [More Information]
  • Christopherson, R. (2008). CD antigens. In Schwab M (Eds.), Encyclopedia of Cancer. Springer.
  • Henrich, S., Mactier, S., Crossett, B., Mulligan, S., Christopherson, R. (2008). Fludarabine induces differential expression of proteins in human leukemia and lymphoma cells. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 27, 634-640. [More Information]
  • Henrich, S., Christopherson, R. (2008). Multiple forms of nuclear p53 formed in human Raji and MEC1 cells treated with fludarabine. Leukemia, 22(3), 657-660. [More Information]
  • Ellmark, P., Hogerkorp, C., Ek, S., Belov, L., Berglund, M., Rosenquist, R., Christopherson, R., Borrebaeck, C. (2008). Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays. Cancer Letters, 265(1), 98-106. [More Information]
  • Langley, D., Shojaei, M., Chan, C., Lok, H., Mackay, J., Traut, T., Guss, M., Christopherson, R. (2008). Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum. Biochemistry, 47(12), 3842-3854. [More Information]

2007

  • Mactier, S., Crossett, B., Christopherson, R. (2007). Differentially expressed cytosolic proteins in human leukemia and lymphoma cell lines correlate with lineages and functions. Biochimica et Biophysica Acta. Proteins and Proteomics, 1774 (9), 1173-1183. [More Information]
  • Henrich, S., Crossett, B., Christopherson, R. (2007). Differentially expressed nuclear proteins in human CCRF-CEM, HL-60, MECA and Raji cells correlate with cellular properties. Proteomics - Clinical Applications, 1(10), 1252-1265. [More Information]
  • Lee, M., Maher, M., Christopherson, R., Guss, M. (2007). Kinetic and Structural Analysis of Mutant Escherichia coli Dihydroorotases: A Flexible Loop Stabilizes the Transition State(,). Biochemistry, 46(37), 10538-10550. [More Information]
  • Leong, S., Christopherson, R., Baxter, R. (2007). Profiling of apoptotic changes in human breast cancer cells using SELDI-TOF mass spectrometry. Cellular Physiology and Biochemistry, 20(5), 579-590. [More Information]
  • Lee, M., Chan, C., Graham, S., Christopherson, R., Guss, M., Maher, M. (2007). Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: Implications for loop movement in inhibitor design. Journal of Molecular Biology, 370(5), 812-825. [More Information]
  • Ellmark, P., Woolfson, A., Belov, L., Christopherson, R. (2007). The applicability of a cluster of differentiation monoclonal antibody microarray to the diagnosis of human disease. Methods in Molecular Biology, 439(14). [More Information]
  • Henrich, S., Cordwell, S., Crossett, B., Baker, M., Christopherson, R. (2007). The nuclear proteome and DNA-binding fraction of human Raji lymphoma cells. Biochimica et Biophysica Acta. Proteins and Proteomics, 1774 (4), 413-432. [More Information]

2006

  • Anderson, M., Cleland, W., Huang, D., Chan, C., Shojaei, M., Christopherson, R. (2006). 13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus. Biochemistry, 45(23), 7132-7139. [More Information]
  • Belov, L., Mulligan, S., Barber, N., Woolfson, A., Scott, M., Stoner, K., Chrisp, J., Sewell, W., Bradstock, K., Bendall, L., Sartor, M., Young, G., Wiley, J., Christopherson, R., et al (2006). Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray. British Journal of Haematology, 135(2), 184-97. [More Information]
  • Christopherson, R., Stoner, K., Barber, N., Belov, L., Woolfson, A., Scott, M., Bendall, L., Mulligan, S. (2006). Classification of AML using a monoclonal antibody microarray. Methods in molecular medicine, 125, 241-251. [More Information]
  • Ellmark, P., Belov, L., Huang, P., Lee, C., Solomon, M., Morgan, D., Christopherson, R. (2006). Multiplex detection of surface molecules on colorectal cancers. Proteomics, 6(6), 1791-1802. [More Information]
  • Christopherson, R. (2006). Nucleotide Biosynthesis. In Ganten, Detlev; Ruckpaul, Klaus (Eds.), Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine, (pp. 1333-1337). NY United States: Springer.
  • Woolfson, A., Ellmark, P., Chrisp, J., Scott, M., Christopherson, R. (2006). The application of CD antigen proteomics to pharmacogenomics. Pharmacogenomics, 7(5), 759-771. [More Information]
  • Huang, D., Kaplan, J., Menz, R., Katis, V., Wake, R., Zhao, F., Wolfenden, R., Christopherson, R. (2006). Thermodynamic Analysis of Catalysis by the Dihydroorotases from Hamster and Bacillus caldolyticus, As Compared with the Uncatalyzed Reaction. Biochemistry, 45(27), 8275-8283. [More Information]

2005

  • Woolfson, A., Stebbing, J., Tom, B., Stoner, K., Gilks, W., Kreil, D., Mulligan, S., Belov, L., Chrisp, J., Errington, W., Christopherson, R., et al (2005). Conservation of unique cell-surface CD antigen mosaics in HIV-1-infected individuals. Blood, 106(3), 1003-1007. [More Information]
  • Lee, M., Chan, C., Guss, M., Christopherson, R., Maher, M. (2005). Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits. Journal of Molecular Biology, 348(3), 523-533. [More Information]
  • Georgiou, J., Skarratt, K., Fuller, S., Martin, C., Christopherson, R., Wiley, J., Sluyter, R. (2005). Human Epidermal and Monocyte-Derived Langerhans Cells Express Functional P2X7 Receptors. The Journal of Investigative Dermatology, 125(3), 482-490. [More Information]
  • Sluyter, R., Georgiou, J., Skarratt, K., Fuller, S., Martin, C., Christopherson, R., Wiley, J. (2005). Human epidermal and monocyte-derived langerhans cells express functional P2X7 receptors. 35th Annual Scientific Meeting of the Australasian Society for Immunology 2005, Denmark: Blackwell Munksgaard.
  • White, S., Belov, L., Barber, N., Hodgkin, P., Christopherson, R. (2005). Immunophenotypic changes induced on human HL60 leukaemia cells by 1alpha,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate. Leukemia Research: clinical and laboratory studies, 29(10), 1141-1151. [More Information]
  • Belov, L., Huang, P., Chrisp, J., Mulligan, S., Christopherson, R. (2005). Screening microarrays of novel monoclonal antibodies for binding to T-, B- and myeloid leukaemia cells. Journal of Immunological Methods, 305(1), 10-19. [More Information]

2004

  • Kamal, M., Christopherson, R. (2004). Accumulation Of 5-Phosphoribosyl-1-Pyrophosphate In Human CCRF-CEM Leukaemia Cells Treated With Antifolates. The International Journal of Biochemistry and Cell Biology, 36(3), 545-551. [More Information]
  • Christopherson, R., Cinquin, O., Shojaei, M., Kuehn, D., Menz, R. (2004). Cloning and expression of malarial pyrimidine enzymes. Nucleosides, Nucleotides and Nucleic Acids: An International Journal for Rapid Communication, 23(8&9), 1459-1465.
  • Wilson, P., Mulligan, S., Christopherson, R. (2004). Metabolic Response Patterns Of Nucleotides In B-Cell Chronic Lymphocytic Leukaemias To Cladribine, Fludarabine And Deoxycoformycin. Leukemia Research: clinical and laboratory studies, 28(7), 725-731.

2003

  • Maher, M., Huang, D., Guss, M., Collyer, C., Christopherson, R. (2003). Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form. Acta Crystallographica. Section D: Biological Crystallography, 59(2), 381-384.
  • Belov, L., Huang, P., Barber, N., Mulligan, S., Christopherson, R. (2003). Identification of repertoires of surface antigens on leukemias using an antibody mircroarray. Proteomics, 3(11), 2147-2154. [More Information]

2002

  • Lal, S., Christopherson, R., Dos Remedios, C. (2002). Antibody arrays: an embryonic but rapidly growing technology. Drug Discovery Today, 7(18 Supplement), S143-S149.
  • Christopherson, R., Lyons, S., Wilson, P. (2002). Inhibitors of de Novo nucleotide biosynthesis as drugs. Accounts Of Chemical Research, 35(11), 961-971.
  • Menz, R., Cinquin, O., Christopherson, R. (2002). The identification, cloning and functional expression of the gene encoding orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum. Annals Of Tropical Medicine And Parasitology, 96(5), 469-476.

2001

  • Christopherson, R., Menz, R., Cinquin, O. (2001). A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli. Molecular and Biochemical Parasitology, 117, 245-247.
  • Belov, L., de la Vega, O., Dos Remedios, C., Christopherson, R., Mulligan,, S. (2001). Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer Research, 61(11), 4483-4489.

To update your profile click here. For support on your academic profile contact .