Dr Sean Humphrey

Postdoctoral Research Fellow
School of Life and Environmental Sciences

D17 - Charles Perkins Centre
The University of Sydney

Telephone +61 2 8627 4315

Website Level 5 West, D17 - Charles Perkins Centre

Current research students

Project title Research student
Pancreatic Islet Cell Atlas Sheyda NAGHILOO

Selected grants

2017

  • EasyPhos: Scalable and high performace phosphoproteomics workflow; Humphrey S; DVC Research/Bridging Support Grant.

2016

  • Ultra-high pressure system for proteomic analysis of a multi-ethnic cohort to identify biomarkers for type-2 diabetes; James D, Parker B, Humphrey S; Ian Potter Foundation/Medical Research.

Selected publications

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Journals

  • Krycer, J., Fazakerley, D., Cater, R., Thomas, K., Naghiloo, S., Burchfield, J., Humphrey, S., Vandenberg, R., Ryan, R., James, D. (2017). The amino acid transporter SLC1A3 is plasma membrane-localised in adipocytes and its activity is insensitive to insulin. FEBS Letters, 591(2), 322-330. [More Information]
  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Coster, A., Stoeckli, J., James, D. (2016). Hyperactivation of the Insulin Signaling Pathway Improves Intracellular Proteostasis by Coordinately Up-regulating the Proteostatic Machinery in Adipocytes. Journal of Biological Chemistry, 291(49), 25629-25640. [More Information]
  • Yang, P., Patrick, E., Humphrey, S., Ghazanfar, S., James, D., Jothi, R., Yang, J. (2016). KinasePA: Phosphoproteomics data annotation using hypothesis driven kinase perturbation analysis. Proteomics, 16(13), 1868-1871. [More Information]
  • Zaman, S., Choudhury, M., Jiang, J., Srivastava, P., Mohanty, B., Danielson, C., Humphrey, S., Jazwinski, S., Bastia, D. (2016). Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Molecular and Cellular Biology, 36(10), 1451-1463. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stoeckli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17(1), 29-36. [More Information]
  • Yang, P., Humphrey, S., James, D., Yang, J., Jothi, R. (2016). Positive-unlabeled ensemble learning for kinase substrate prediction from dynamic phosphoproteomics data. Bioinformatics, 32(2), 252-259. [More Information]
  • Domanova, W., Krycer, J., Chaudhuri, R., Yang, P., Vafaee, F., Fazakerley, D., Humphrey, S., James, D., Kuncic, Z. (2016). Unraveling Kinase Activation Dynamics Using Kinase-Substrate Relationships from Temporal Large-Scale Phosphoproteomics Studies. PloS One, 11(6), 1-14. [More Information]
  • Yang, G., Murashige, D., Humphrey, S., James, D. (2015). A Positive Feedback Loop between Akt and mTORC2 via SIN1 Phosphorylation. Cell Reports, 12(6), 937-943. [More Information]
  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • Humphrey, S., Azimifar, S., Mann, M. (2015). High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics. Nature Biotechnology, 33(9), 990-995. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Humphrey, S., James, D., Mann, M. (2015). Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation. Trends in Endocrinology and Metabolism, 26(12), 676-687. [More Information]
  • Parker, B., Yang, G., Humphrey, S., Chaudhuri, R., Ma, X., Peterman, S., James, D. (2015). Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Science Signaling, 8(380), 1-8. [More Information]
  • Humphrey, S., Yang, G., Yang, P., Fazakerley, D., Stockli, J., Yang, J., James, D. (2013). Dynamic adipocyte phosphoproteome reveals that akt directly regulates mTORC2. Cell Metabolism, 17(6), 1009-1020. [More Information]
  • Yang, P., Humphrey, S., Fazakerley, D., Prior, M., Yang, G., James, D., Yang, J. (2012). Re-Fraction: A machine learning approach for deterministic identification of protein homologues and splice variants in large-scale MS-based proteomics. Journal of Proteome Research, 11(5), 3035-3045. [More Information]
  • Davey, J., Humphrey, S., Junutula, J., Mishra, A., Lambright, D., James, D., Stockli, J. (2012). TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic, 13(10), 1429-1441. [More Information]
  • Humphrey, S., James, D. (2012). Uncaging Akt. Science Signaling, 5(223). [More Information]
  • Prior, M., Larance, M., Lawrence, R., Soul, J., Humphrey, S., Burchfield, J., Kistler, C., Davey, J., La-Borde, P., Buckley, M., James, D., et al (2011). Quantitative Proteomic Analysis of the Adipocyte Plasma Membrane. Journal of Proteome Research, 10(11), 4970-4982. [More Information]

2017

  • Krycer, J., Fazakerley, D., Cater, R., Thomas, K., Naghiloo, S., Burchfield, J., Humphrey, S., Vandenberg, R., Ryan, R., James, D. (2017). The amino acid transporter SLC1A3 is plasma membrane-localised in adipocytes and its activity is insensitive to insulin. FEBS Letters, 591(2), 322-330. [More Information]

2016

  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Coster, A., Stoeckli, J., James, D. (2016). Hyperactivation of the Insulin Signaling Pathway Improves Intracellular Proteostasis by Coordinately Up-regulating the Proteostatic Machinery in Adipocytes. Journal of Biological Chemistry, 291(49), 25629-25640. [More Information]
  • Yang, P., Patrick, E., Humphrey, S., Ghazanfar, S., James, D., Jothi, R., Yang, J. (2016). KinasePA: Phosphoproteomics data annotation using hypothesis driven kinase perturbation analysis. Proteomics, 16(13), 1868-1871. [More Information]
  • Zaman, S., Choudhury, M., Jiang, J., Srivastava, P., Mohanty, B., Danielson, C., Humphrey, S., Jazwinski, S., Bastia, D. (2016). Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Molecular and Cellular Biology, 36(10), 1451-1463. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stoeckli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17(1), 29-36. [More Information]
  • Yang, P., Humphrey, S., James, D., Yang, J., Jothi, R. (2016). Positive-unlabeled ensemble learning for kinase substrate prediction from dynamic phosphoproteomics data. Bioinformatics, 32(2), 252-259. [More Information]
  • Domanova, W., Krycer, J., Chaudhuri, R., Yang, P., Vafaee, F., Fazakerley, D., Humphrey, S., James, D., Kuncic, Z. (2016). Unraveling Kinase Activation Dynamics Using Kinase-Substrate Relationships from Temporal Large-Scale Phosphoproteomics Studies. PloS One, 11(6), 1-14. [More Information]

2015

  • Yang, G., Murashige, D., Humphrey, S., James, D. (2015). A Positive Feedback Loop between Akt and mTORC2 via SIN1 Phosphorylation. Cell Reports, 12(6), 937-943. [More Information]
  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • Humphrey, S., Azimifar, S., Mann, M. (2015). High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics. Nature Biotechnology, 33(9), 990-995. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Humphrey, S., James, D., Mann, M. (2015). Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation. Trends in Endocrinology and Metabolism, 26(12), 676-687. [More Information]
  • Parker, B., Yang, G., Humphrey, S., Chaudhuri, R., Ma, X., Peterman, S., James, D. (2015). Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Science Signaling, 8(380), 1-8. [More Information]

2013

  • Humphrey, S., Yang, G., Yang, P., Fazakerley, D., Stockli, J., Yang, J., James, D. (2013). Dynamic adipocyte phosphoproteome reveals that akt directly regulates mTORC2. Cell Metabolism, 17(6), 1009-1020. [More Information]

2012

  • Yang, P., Humphrey, S., Fazakerley, D., Prior, M., Yang, G., James, D., Yang, J. (2012). Re-Fraction: A machine learning approach for deterministic identification of protein homologues and splice variants in large-scale MS-based proteomics. Journal of Proteome Research, 11(5), 3035-3045. [More Information]
  • Davey, J., Humphrey, S., Junutula, J., Mishra, A., Lambright, D., James, D., Stockli, J. (2012). TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic, 13(10), 1429-1441. [More Information]
  • Humphrey, S., James, D. (2012). Uncaging Akt. Science Signaling, 5(223). [More Information]

2011

  • Prior, M., Larance, M., Lawrence, R., Soul, J., Humphrey, S., Burchfield, J., Kistler, C., Davey, J., La-Borde, P., Buckley, M., James, D., et al (2011). Quantitative Proteomic Analysis of the Adipocyte Plasma Membrane. Journal of Proteome Research, 10(11), 4970-4982. [More Information]

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