student profile: Miss Susannah Hallal


Map

Thesis work

Thesis title: Modulation of Neuroglia Function by Glioblastoma Stem Cell-derived Extracellular Vesicles

Supervisors: Lenka MUNOZ , Michael BUCKLAND , Kimberley KAUFMAN

Thesis abstract:

Glioblastoma multiforme (GBM) is the most common and lethal primary brain tumour in adults. In order to promote tumour progression, GBM cells have been shown to hijack their tumour microenvironment via extracellular vesicle (EV) signaling. EVs are 30-1000 nm membranous vesicles released by all cells and contain molecules that reflect their cell of origin. EVs are gaining immense attention in cancer research for their role in intercellular communication. Glioma stem-like cells (GSC) are proposed to drive the aggressiveness, relapse and treatment resistance of GBM tumours. We have observed the induction of an invasion-like phenotype in normal primary astrocytes treated with GSC-EVs. Characterising the molecular changes in astrocytes in response to GSC-EV will help understand how aggressive GBMs hijack their environment. EVs are also valuable sources of biomarkers. Recently, the Kaufman lab described the most comprehensive GBM-EV proteome signature to date, including 14 EV proteins that correlated with GBM invasiveness. We aim to translate this in vitro signature to clinical specimens to describe new biomarkers predictive of GBM progression and invasion. We have recently identified and characterised a novel source of clinical GBM-EVs, as well as living tumour cells. These derivatives are being routinely collected for ongoing biomarker and intercellular signaling studies.

Selected publications

Download citations: PDF RTF Endnote

Journals

  • Mallawaaratchy, D., Hallal, S., Russell, B., Ly, L., Ebrahimkhani, S., Wei, H., Christopherson, R., Buckland, M., Kaufman (nee Alexander), K. (2017). Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Journal of Neuro-Oncology, 131(2), 233-244. [More Information]

2017

  • Mallawaaratchy, D., Hallal, S., Russell, B., Ly, L., Ebrahimkhani, S., Wei, H., Christopherson, R., Buckland, M., Kaufman (nee Alexander), K. (2017). Comprehensive proteome profiling of glioblastoma-derived extracellular vesicles identifies markers for more aggressive disease. Journal of Neuro-Oncology, 131(2), 233-244. [More Information]

Note: This profile is for a student at the University of Sydney. Views presented here are not necessarily those of the University.