student profile: Miss Taylor Pini


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Thesis work

Thesis title: Identify and apply proteins to improve freezing success and promote fertility of frozen-thawed ram sperm.

Supervisors: Simon DE GRAAF , Tamara LEAHY

Thesis abstract:

Cervical insemination using frozen thawed semen remains a largely unviable option for commercial sheep producers due to its poor success rates. Poor pregnancy rates are thought to stem from a) the damage which sperm incur during the freezing process and b) the complex barrier presented by the ovine cervix and the cervical mucus it produces. Previous research has suggested that seminal plasma, and particularly its proteins, help reduce freezing damage and also impart an improved ability of sperm to transit the cervix. The current research project focuses on the mechanisms behind this improvement in hopes that it may be manipulated to improve the success of cervical inseminations using frozen thawed semen.

Selected publications

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Journals

  • Pini, T., Leahy, T., de Graaf, S. (2016). Changes to sperm surface carbohydrates following exposure to seminal plasma and freezing. Animal Reproduction Science, 169, 100-100. [More Information]
  • Pini, T., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Combes-Soia, L., Harichaux, G., Rickard, J., Druart, X., de Graaf, S. (2016). Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma. Journal of Proteome Research, 15(10), 3700-3711. [More Information]
  • Rickard, J., Pini, T., Soleilhavoup, C., Cognie, J., Bathgate, R., Lynch, G., Evans, G., Maxwell, W., Druart, X., de Graaf, S. (2014). Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction, 148, 469-478. [More Information]

Conferences

  • Rickard, J., Pini, T., Leahy, T., de Graaf, S. (2016). Species specific sorting protocol required for the production of high quality chill- and frozen-stored sex-sorted ram sperm. 18th International Congress on Animal Reproduction, N/A: N/A.
  • de Graaf, S., Rickard, J., Pini, T., Maddison, J., Druart, X., Leahy, T. (2014). Emerging Roles of Seminal Plasma in Sperm Function. 9th Biennial Conference of the Association for Applied Animal Andrology, Newcastle: Association for Applied Animal Andrology.
  • Rickard, J., Maddison, J., Pini, T., Bathgate, R., Evans, G., Maxwell, W., Lynch, G., Druart, X., de Graaf, S. (2013). Exposure to seminal plasma aids the migration of epididymal ram spermatozoa through the cervix. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.

2016

  • Pini, T., Leahy, T., de Graaf, S. (2016). Changes to sperm surface carbohydrates following exposure to seminal plasma and freezing. Animal Reproduction Science, 169, 100-100. [More Information]
  • Pini, T., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Combes-Soia, L., Harichaux, G., Rickard, J., Druart, X., de Graaf, S. (2016). Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma. Journal of Proteome Research, 15(10), 3700-3711. [More Information]
  • Rickard, J., Pini, T., Leahy, T., de Graaf, S. (2016). Species specific sorting protocol required for the production of high quality chill- and frozen-stored sex-sorted ram sperm. 18th International Congress on Animal Reproduction, N/A: N/A.

2014

  • de Graaf, S., Rickard, J., Pini, T., Maddison, J., Druart, X., Leahy, T. (2014). Emerging Roles of Seminal Plasma in Sperm Function. 9th Biennial Conference of the Association for Applied Animal Andrology, Newcastle: Association for Applied Animal Andrology.
  • Rickard, J., Pini, T., Soleilhavoup, C., Cognie, J., Bathgate, R., Lynch, G., Evans, G., Maxwell, W., Druart, X., de Graaf, S. (2014). Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction, 148, 469-478. [More Information]

2013

  • Rickard, J., Maddison, J., Pini, T., Bathgate, R., Evans, G., Maxwell, W., Lynch, G., Druart, X., de Graaf, S. (2013). Exposure to seminal plasma aids the migration of epididymal ram spermatozoa through the cervix. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.

Note: This profile is for a student at the University of Sydney. Views presented here are not necessarily those of the University.