Dr Kumudika de Silva

BAppSc (Hons), PhD
Research Fellow  

C01A - Jl Shute - Camden
The University of Sydney

Telephone +61 2 9036 7737
Fax +61 2 9351 1693

Website Johne's Disease Sydney

Research interests

Using the early cellular immune response to predict disease resistance and susceptibility and vaccine effectivenessin sheep and cattle, specifically in relation to Johne's disease (a mycobacterial infection).

Associations

Awards and honours

2015

Women at Sydney Vice Chancellor's Sponsorship Program

Selected grants

2011

  • OJD/BJD: Diagnostic, predictive and preventative tools for Johnes disease in sheep and cattle; Whittington R, Begg D, de Silva K, Plain K, Purdie (Williams) A, Dhand N; Meat and Livestock Australia Ltd/Livestock Production Research and Development Program: Strategic and Applied Research Funding.

Selected publications

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Journals

  • de Silva, K., Plain, K., Begg, D., Purdie (Williams), A., Whittington, R. (2015). CD4+ T-cells, T-cells and B-cells are associated with lack of vaccine protection in Mycobacterium avium subspecies paratuberculosis infection. Vaccine, 33, 149-155. [More Information]
  • Plain, K., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2015). Efficient, validated method for the detection of mycobacterial growth in liquid culture media using bead-beating, magnetic particle nucleic acid isolation and quantitative PCR. Journal of Clinical Microbiology, 53(4), 1121-1128. [More Information]
  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Dhand, N., Whittington, R. (2015). Specific faecal antibody responses in sheep infected with Mycobacterium avium subspecies paratuberculosis. Veterinary Immunology and Immunopathology, 166(3-4), 125-131. [More Information]
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Begg, D., Whittington, R., Purdie (Williams), A. (2014). Expression of genes associated with cholesterol and lipid metabolism identified as a novel pathway in the early pathogenesis of Mycobacterium avium subspecies paratuberculosis - infection in cattle. Veterinary Immunology and Immunopathology, 160(3-4), 147-157. [More Information]
  • Plain, K., Marsh, I., Waldron, A., Galea, F., Whittington, A., Saunders, V., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2014). High-Throughput Direct Faecal PCR Assay to detection of Mycobacterium avium subspecies paratuberculosis in sheep and cattle. Journal of Clinical Microbiology, 52(3), 745-757. [More Information]
  • Bhardwaj, V., Dhungyel, O., de Silva, K., Whittington, R. (2014). Investigation of immunity in sheep following footrot infection and vaccination. Vaccine, 32(51), 6979-6985. [More Information]
  • Gurung, R., Begg, D., Purdie (Williams), A., de Silva, K., Bannantine, J., Whittington, R. (2014). Lymphoproliferative and Gamma Interferon responses to stress-regulated Mycobacterium avium subspecies paratuberculosis recombinant proteins. Clinical and Vaccine Immunology, 21(6), 831-837. [More Information]
  • de Silva, K., Browne, S., Begg, D., Whittington, R., Emery, D. (2013). Apoptosis of lymph node and peripheral blood cells in ovine Johne's disease. Veterinary Immunology and Immunopathology, 156(1-2), 82-90. [More Information]
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2013). Can early host responses to mycobacterial infection predict eventual disease outcomes? Preventive Veterinary Medicine, 112, 203-212. [More Information]
  • Thirunavukkarasu, S., Plain, K., Eckstein, T., de Silva, K., Whittington, R. (2013). Cellular and humoral immunogenicity of Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigens. Research In Veterinary Science, 95, 123-129. [More Information]
  • Whittington, R., Whittington, A., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Plain, K. (2013). Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium. Journal of Clinical Microbiology, 51(12), 3993-4000. [More Information]
  • Thirunavukkarasu, S., de Silva, K., Whittington, R., Plain, K. (2013). In vivo and in vitro expression pattern of Toll-like receptors in Mycobacterium avium subspecies paratuberculosis infection. Veterinary Immunology and Immunopathology, 156, 20-31. [More Information]
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2012). Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection. Veterinary Immunology and Immunopathology, 148(1-2), 29-47. [More Information]
  • Plain, K., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Enhancement of the interferon gamma assay to detect paratuberculosis using interleukin-7 and interleukin-12 potentiation. Veterinary Immunology and Immunopathology, 149(1-2), 28-37. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2012). Expression of genes associated with the antigen presentation and processing pathway are consistently regulated in early Mycobacterium avium subsp. paratuberculosis infection. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 35(2), 151-162. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2011). Candidate gene and genome-wide association studies of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep: A review. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 34(3), 197-208. [More Information]
  • Begg, D., de Silva, K., Carter, N., Plain, K., Purdie (Williams), A., Whittington, R. (2011). Does a Th1 over Th2 dominancy really exist in the early stages of Mycobacterium avium subspecies paratuberculosis infections? Immunobiology, 216(7), 840-846. [More Information]
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2011). Indoleamine 2,3-Dioxygenase, Tryptophan Catabolism, and Mycobacterium avium subsp. paratuberculosis: a model for Chronic Mycobacterial Infection. Infection and Immunity, 79(9), 3821-3832. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2011). The interleukin 10 response in ovine Johne's disease. Veterinary Immunology and Immunopathology, 139(1), 10-16. [More Information]
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2010). Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis. Veterinary Microbiology, 141, 301-311. [More Information]
  • de Silva, K., Begg, D., Carter, N., Taylor, D., Di Fiore, L., Whittington, R. (2010). The early lympocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis  compared to infection status. Immunobiology, 215(1), 12-25. [More Information]
  • Plain, K., Purdie (Williams), A., Begg, D., de Silva, K., Whittington, R. (2010). Toll-like receptor (TLR)6 and TLR1 differentiation in gene expression studies of Johne's disease. Veterinary Immunology and Immunopathology, 137(1-2), 142-148. [More Information]
  • Begg, D., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D., Jungersen, G., Whittington, R. (2009). Enzyme-linked immunospot: An alternative method for the detection of interferon gamma in Johne's disease. Journal of Veterinary Diagnostic Investigation, 21(2), 187-196.
  • Zhong, L., Di Fiore, L., Taylor, D., Begg, D., de Silva, K., Whittington, R. (2009). Identification of differentially expressed genes in ileum, intestinal lymph node and peripheral blood mononuclear cells of sheep infected with Mycobacterium avium subsp. paratuberculosis using differential display polymerase chain reaction. Veterinary Immunology and Immunopathology, 131(3-4), 177-189. [More Information]
  • Taylor, D., Zhong, L., Begg, D., de Silva, K., Whittington, R. (2008). Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep. Veterinary Immunology and Immunopathology, 124, 132-151. [More Information]
  • Taylor, D., Thomson, P., de Silva, K., Whittington, R. (2007). Validation of endogenous reference genes for expression profiling of RAW264.7 cells infected with Mycobacterium avium subsp. paratuberculosis by quantitive PCR. Veterinary Immunology and Immunopathology, 115, 43-55.
  • de Silva, K., Daud, A., Deng, J., Jones, S., Gamelli, R., Shankar, R. (2003). Prostaglandin E2 mediates growth arrest in NFS-60 cells by down-regulating interleukin-6 receptor expression. Biochemical Journal, 370(1), 315-321.

Conferences

  • Plain, K., Marsh, I., Begg, D., Purdie (Williams), A., de Silva, K., Whittington, R. (2014). A new molecular diagnostic test for Johne's disease; from lab to national uptake. 12th International Colloquium on Paratuberculosis, Italy: N/A.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2014). Consistent gene expression panels identified at early time points that predict clinical outcomes of JD in sheep. 12th International Colloquium on Paratuberculosis, Italy: N/A.
  • Bhardwaj, V., de Silva, K., Dhungyel, O., Whittington, R. (2011). Host Response to Footrot Infection and Vaccination in Sheep. Australian Society for Microbiology Annual Scientific Meeting, N/A: N/A.
  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Whittington, R. (2010). Does a Th1 over Th2 dominance exist in the early stages of Mycobacterium avium subsp. paratuberculosis infections? 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2010). Immune correlates of protection and susceptibility to mycobacterial disease. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Kawaji, S., Plain, K., Whittington, R. (2010). Immune profile and faecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne’s disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K. (2010). Immune profile and fecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne's disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Immunological characteristics of paratuberculosis as a mycobacterial infection. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Mycobacterial infection – lessons from tuberculosis and paratuberculosis. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2010). Role of IDO and tryptophan catabolism in chronic mycobacterial disease pathogenesis. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2009). Development of an experimental infection model for Johne's disease using a lyophilised pure culture seedstock of Mycobacterium avium subspecies paratuberculosis. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Begg, D., Plain, K., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an Experimental Infection Model in Cattle using a Low Passage Seed Stock of MAP. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Plain, K., Begg, D., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an experimental infection protocol in cattle using a low passage cultured strain of MAP. 10th International Colloquium on Paratuberculosis, USA: University of Minnesota.
  • de Silva, K., Begg, D., Whittington, R. (2009). The early IL-10 response in ovine Johne's disease. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Taylor, D., Zhong, L., Di Fiore, L., Begg, D., de Silva, K., Whittington, R. (2008). Analysis of Toll-like receptor gene expression in Mycobacterium paratuberculosis infected sheep. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Browne, S., de Silva, K., Begg, D., Whittington, R., Emery, D. (2008). Apoptosis of mononuclear cells in experimental and natural ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2008). Experimental infection of sheep with M.avium subspecies paratuberculosis: a brief review and introduction to an Australian ovine challenge model. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • de Silva, K., Begg, D., Taylor, D., Di Fiore, L., Whittington, R. (2008). Proliferation of lymphocyte subsets in experimental ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2008). Seeing spots, developing an IFN-gamma ELISPOT assay to detect ovine M. avium subspecies paratuberculosis infection. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Whittington, R. (2008). The IFN-g to antibody switch - does it really happen in Mycobacterium paratuberculosis infections? The 38th Australasian Society for Immunology Annual Conference ASI 2008, Australia: Australasian Society for Immunology.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identificaiton of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M.ptb. ) infected sheep. The 8th International Colloquium on Paratuberculosis, N/A: N/A.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identification of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M. ptb.) infected sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2006). Optimisation of the interferon-gamma assay to maximise responses to M. ptb. antigen in sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2006). Seeing spots, using the ELISPOT assay to detect M. avium subspecies paratuberculosis infection in sheep. Australasian Society for Immunology Conference 2006, Australia: Australasian Society for Immunology.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptosis in sheep exposed to Mycobacterium avium paratuberculosis. Apoptosis and Immunity Conference, Melbourne, Victoria, Australia: Peter MacCallum Cancer Centre.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptotic responses during the pathogenesis of ovice Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • de Silva, K., Begg, D., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2005). Early cellular responses in ovine Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Begg, D., Bosward, K., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). ELISPOT - new methods for detecting IFN-y. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2005). Gene expression and survival of M. paratuberculosis infected macrophages. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., McGregor, H., Whittington, R. (2005). Gene expression signals from the host. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). Manipulation of the Interferon - gamma assay to maximise responses to M. ptb antigen in sheep. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., Gumber, S., de Silva, K., Bower (Goldsmith), K., Di Fiore, L., Begg, D., Whittington, R. (2005). Molecular approaches to studying the host-parasite interaction. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Ovine apoptotic responses to Mycobacterium avium paratuberculosis. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • de Silva, K., Carter, N., Taylor, D., Di Fiore, L., Whittington, R., Emery, D. (2005). Proliferative responses in peripheral blood and lymph node cells in the early stages of ovine Johne's disease. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.

2015

  • de Silva, K., Plain, K., Begg, D., Purdie (Williams), A., Whittington, R. (2015). CD4+ T-cells, T-cells and B-cells are associated with lack of vaccine protection in Mycobacterium avium subspecies paratuberculosis infection. Vaccine, 33, 149-155. [More Information]
  • Plain, K., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2015). Efficient, validated method for the detection of mycobacterial growth in liquid culture media using bead-beating, magnetic particle nucleic acid isolation and quantitative PCR. Journal of Clinical Microbiology, 53(4), 1121-1128. [More Information]
  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Dhand, N., Whittington, R. (2015). Specific faecal antibody responses in sheep infected with Mycobacterium avium subspecies paratuberculosis. Veterinary Immunology and Immunopathology, 166(3-4), 125-131. [More Information]

2014

  • Plain, K., Marsh, I., Begg, D., Purdie (Williams), A., de Silva, K., Whittington, R. (2014). A new molecular diagnostic test for Johne's disease; from lab to national uptake. 12th International Colloquium on Paratuberculosis, Italy: N/A.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2014). Consistent gene expression panels identified at early time points that predict clinical outcomes of JD in sheep. 12th International Colloquium on Paratuberculosis, Italy: N/A.
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Begg, D., Whittington, R., Purdie (Williams), A. (2014). Expression of genes associated with cholesterol and lipid metabolism identified as a novel pathway in the early pathogenesis of Mycobacterium avium subspecies paratuberculosis - infection in cattle. Veterinary Immunology and Immunopathology, 160(3-4), 147-157. [More Information]
  • Plain, K., Marsh, I., Waldron, A., Galea, F., Whittington, A., Saunders, V., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2014). High-Throughput Direct Faecal PCR Assay to detection of Mycobacterium avium subspecies paratuberculosis in sheep and cattle. Journal of Clinical Microbiology, 52(3), 745-757. [More Information]
  • Bhardwaj, V., Dhungyel, O., de Silva, K., Whittington, R. (2014). Investigation of immunity in sheep following footrot infection and vaccination. Vaccine, 32(51), 6979-6985. [More Information]
  • Gurung, R., Begg, D., Purdie (Williams), A., de Silva, K., Bannantine, J., Whittington, R. (2014). Lymphoproliferative and Gamma Interferon responses to stress-regulated Mycobacterium avium subspecies paratuberculosis recombinant proteins. Clinical and Vaccine Immunology, 21(6), 831-837. [More Information]

2013

  • de Silva, K., Browne, S., Begg, D., Whittington, R., Emery, D. (2013). Apoptosis of lymph node and peripheral blood cells in ovine Johne's disease. Veterinary Immunology and Immunopathology, 156(1-2), 82-90. [More Information]
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2013). Can early host responses to mycobacterial infection predict eventual disease outcomes? Preventive Veterinary Medicine, 112, 203-212. [More Information]
  • Thirunavukkarasu, S., Plain, K., Eckstein, T., de Silva, K., Whittington, R. (2013). Cellular and humoral immunogenicity of Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigens. Research In Veterinary Science, 95, 123-129. [More Information]
  • Whittington, R., Whittington, A., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Plain, K. (2013). Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium. Journal of Clinical Microbiology, 51(12), 3993-4000. [More Information]
  • Thirunavukkarasu, S., de Silva, K., Whittington, R., Plain, K. (2013). In vivo and in vitro expression pattern of Toll-like receptors in Mycobacterium avium subspecies paratuberculosis infection. Veterinary Immunology and Immunopathology, 156, 20-31. [More Information]

2012

  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2012). Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection. Veterinary Immunology and Immunopathology, 148(1-2), 29-47. [More Information]
  • Plain, K., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Enhancement of the interferon gamma assay to detect paratuberculosis using interleukin-7 and interleukin-12 potentiation. Veterinary Immunology and Immunopathology, 149(1-2), 28-37. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2012). Expression of genes associated with the antigen presentation and processing pathway are consistently regulated in early Mycobacterium avium subsp. paratuberculosis infection. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 35(2), 151-162. [More Information]

2011

  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2011). Candidate gene and genome-wide association studies of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep: A review. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 34(3), 197-208. [More Information]
  • Begg, D., de Silva, K., Carter, N., Plain, K., Purdie (Williams), A., Whittington, R. (2011). Does a Th1 over Th2 dominancy really exist in the early stages of Mycobacterium avium subspecies paratuberculosis infections? Immunobiology, 216(7), 840-846. [More Information]
  • Bhardwaj, V., de Silva, K., Dhungyel, O., Whittington, R. (2011). Host Response to Footrot Infection and Vaccination in Sheep. Australian Society for Microbiology Annual Scientific Meeting, N/A: N/A.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2011). Indoleamine 2,3-Dioxygenase, Tryptophan Catabolism, and Mycobacterium avium subsp. paratuberculosis: a model for Chronic Mycobacterial Infection. Infection and Immunity, 79(9), 3821-3832. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2011). The interleukin 10 response in ovine Johne's disease. Veterinary Immunology and Immunopathology, 139(1), 10-16. [More Information]

2010

  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Whittington, R. (2010). Does a Th1 over Th2 dominance exist in the early stages of Mycobacterium avium subsp. paratuberculosis infections? 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2010). Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis. Veterinary Microbiology, 141, 301-311. [More Information]
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2010). Immune correlates of protection and susceptibility to mycobacterial disease. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Kawaji, S., Plain, K., Whittington, R. (2010). Immune profile and faecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne’s disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K. (2010). Immune profile and fecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne's disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Immunological characteristics of paratuberculosis as a mycobacterial infection. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Mycobacterial infection – lessons from tuberculosis and paratuberculosis. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2010). Role of IDO and tryptophan catabolism in chronic mycobacterial disease pathogenesis. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Carter, N., Taylor, D., Di Fiore, L., Whittington, R. (2010). The early lympocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis  compared to infection status. Immunobiology, 215(1), 12-25. [More Information]
  • Plain, K., Purdie (Williams), A., Begg, D., de Silva, K., Whittington, R. (2010). Toll-like receptor (TLR)6 and TLR1 differentiation in gene expression studies of Johne's disease. Veterinary Immunology and Immunopathology, 137(1-2), 142-148. [More Information]

2009

  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2009). Development of an experimental infection model for Johne's disease using a lyophilised pure culture seedstock of Mycobacterium avium subspecies paratuberculosis. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Begg, D., Plain, K., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an Experimental Infection Model in Cattle using a Low Passage Seed Stock of MAP. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Plain, K., Begg, D., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an experimental infection protocol in cattle using a low passage cultured strain of MAP. 10th International Colloquium on Paratuberculosis, USA: University of Minnesota.
  • Begg, D., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D., Jungersen, G., Whittington, R. (2009). Enzyme-linked immunospot: An alternative method for the detection of interferon gamma in Johne's disease. Journal of Veterinary Diagnostic Investigation, 21(2), 187-196.
  • Zhong, L., Di Fiore, L., Taylor, D., Begg, D., de Silva, K., Whittington, R. (2009). Identification of differentially expressed genes in ileum, intestinal lymph node and peripheral blood mononuclear cells of sheep infected with Mycobacterium avium subsp. paratuberculosis using differential display polymerase chain reaction. Veterinary Immunology and Immunopathology, 131(3-4), 177-189. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2009). The early IL-10 response in ovine Johne's disease. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.

2008

  • Taylor, D., Zhong, L., Di Fiore, L., Begg, D., de Silva, K., Whittington, R. (2008). Analysis of Toll-like receptor gene expression in Mycobacterium paratuberculosis infected sheep. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Browne, S., de Silva, K., Begg, D., Whittington, R., Emery, D. (2008). Apoptosis of mononuclear cells in experimental and natural ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2008). Experimental infection of sheep with M.avium subspecies paratuberculosis: a brief review and introduction to an Australian ovine challenge model. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • de Silva, K., Begg, D., Taylor, D., Di Fiore, L., Whittington, R. (2008). Proliferation of lymphocyte subsets in experimental ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2008). Seeing spots, developing an IFN-gamma ELISPOT assay to detect ovine M. avium subspecies paratuberculosis infection. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Whittington, R. (2008). The IFN-g to antibody switch - does it really happen in Mycobacterium paratuberculosis infections? The 38th Australasian Society for Immunology Annual Conference ASI 2008, Australia: Australasian Society for Immunology.
  • Taylor, D., Zhong, L., Begg, D., de Silva, K., Whittington, R. (2008). Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep. Veterinary Immunology and Immunopathology, 124, 132-151. [More Information]

2007

  • Taylor, D., Thomson, P., de Silva, K., Whittington, R. (2007). Validation of endogenous reference genes for expression profiling of RAW264.7 cells infected with Mycobacterium avium subsp. paratuberculosis by quantitive PCR. Veterinary Immunology and Immunopathology, 115, 43-55.

2006

  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identificaiton of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M.ptb. ) infected sheep. The 8th International Colloquium on Paratuberculosis, N/A: N/A.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identification of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M. ptb.) infected sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2006). Optimisation of the interferon-gamma assay to maximise responses to M. ptb. antigen in sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2006). Seeing spots, using the ELISPOT assay to detect M. avium subspecies paratuberculosis infection in sheep. Australasian Society for Immunology Conference 2006, Australia: Australasian Society for Immunology.

2005

  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptosis in sheep exposed to Mycobacterium avium paratuberculosis. Apoptosis and Immunity Conference, Melbourne, Victoria, Australia: Peter MacCallum Cancer Centre.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptotic responses during the pathogenesis of ovice Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • de Silva, K., Begg, D., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2005). Early cellular responses in ovine Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Begg, D., Bosward, K., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). ELISPOT - new methods for detecting IFN-y. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2005). Gene expression and survival of M. paratuberculosis infected macrophages. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., McGregor, H., Whittington, R. (2005). Gene expression signals from the host. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). Manipulation of the Interferon - gamma assay to maximise responses to M. ptb antigen in sheep. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., Gumber, S., de Silva, K., Bower (Goldsmith), K., Di Fiore, L., Begg, D., Whittington, R. (2005). Molecular approaches to studying the host-parasite interaction. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Ovine apoptotic responses to Mycobacterium avium paratuberculosis. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • de Silva, K., Carter, N., Taylor, D., Di Fiore, L., Whittington, R., Emery, D. (2005). Proliferative responses in peripheral blood and lymph node cells in the early stages of ovine Johne's disease. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.

2003

  • de Silva, K., Daud, A., Deng, J., Jones, S., Gamelli, R., Shankar, R. (2003). Prostaglandin E2 mediates growth arrest in NFS-60 cells by down-regulating interleukin-6 receptor expression. Biochemical Journal, 370(1), 315-321.

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