Dr Kumudika de Silva

BAppSc (Hons), PhD
Research Fellow  

C01A - Jl Shute - Camden
The University of Sydney

Telephone +61 2 9036 7737
Fax +61 2 9351 1693

Biographical details

Kumi completed a Bachelor of Applied Science degree (Hons. Class I) at the University of Canberra, where she studied the oxidation of low density lipoproteins for her Honours project. She then worked for two years as a lecturer in Biochemistry at the Faculty of Medicine, University of Kelaniya, Sri Lanka.

Kumi returned to Australia to undertake doctoral studies at the John Curtin School of Medical Research, Australian National University, Canberra. During her PhD she studied the adhesion and migration of subsets of human mononuclear cells in response to native and minimally oxidised forms of low density lipoprotein. After completing her PhD, Kumi worked as a postdoctoral research fellow at the Burns and Shock Trauma Institute, Loyola University Medical Center, Chicago, USA. Here she studied the effects of prostaglandin E2 on IL-6 receptor expression and signal transduction pathways. She found that PGE2 was able to arrest cell proliferation in an in vitro model by down-regulating expression of the IL-6 receptor as well as subsequent signal transduction and identified cell surface receptors used by PGE2. This work provides an explanation for the granulocytopenia seen in thermal injury and sepsis. Kumi was also involved in studies investigating the response of bone marrow stem cells to injury in a murine model. Kumi joined the Farm Animal and Veterinary Public Health Group in 2002 to study the pathogenesis of Mycobacterium avium subsp. paratuberculosis infection, the cause of Johne¿s disease, in sheep. She is currently involved in investigating the cell-mediated immune response in sheep during the early stages of Johne¿s disease. Kumi also has a long-held interest in scientific communication and is a member of the Society of Editors (NSW).

Selected grants

2011

  • OJD/BJD: Diagnostic, predictive and preventative tools for Johne's disease in sheep and cattle; Whittington R, Begg D, de Silva K, Plain K, Purdie (Williams) A, Dhand N; Meat and Livestock Australia Ltd/Livestock Production Research and Development Program: Strategic and Applied Research Funding.

Selected publications

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Journals

  • Houang, M., Toon, C., Clarkson, A., Sioson, L., de Silva, K., Watson, N., Singh, N., Chou, A., Gill, A. (2014). ALK and ROS1 overexpression is very rare in colorectal adenocarcinoma. Applied Immunohistochemistry and Molecular Morphology, Article in Press.
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Begg, D., Whittington, R., Purdie (Williams), A. (2014). Expression of genes associated with cholesterol and lipid metabolism identified as a novel pathway in the early pathogenesis of Mycobacterium avium subspecies paratuberculosis - infection in cattle. Veterinary Immunology and Immunopathology, 160, 147-157. [More Information]
  • Plain, K., Marsh, I., Waldron, A., Galea, F., Whittington, A., Saunders, V., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2014). High-Throughput Direct Faecal PCR Assay to detect Mycobacterium avium subspecies paratuberculosis in sheep and cattle. Journal of Clinical Microbiology, 52(3), 745-757. [More Information]
  • Gurung, R., Begg, D., Purdie (Williams), A., de Silva, K., Bannantine, J., Whittington, R. (2014). Lymphoproliferative and IFN-y responses to stress-regulated Mycobacterium avium subspecies paratuberculosis recombinant proteins. Clinical and Vaccine Immunology, 21(6), 831-837. [More Information]
  • de Silva, K., Browne, S., Begg, D., Whittington, R., Emery, D. (2013). Apoptosis of lymph node and peripheral blood cells in ovine Johne's disease. Veterinary Immunology and Immunopathology, 156(1-2), 82-90. [More Information]
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2013). Can early host responses to mycobacterial infection predict eventual disease outcomes? Preventive Veterinary Medicine, 112, 203-212. [More Information]
  • Thirunavukkarasu, S., Plain, K., Eckstein, T., de Silva, K., Whittington, R. (2013). Cellular and humoral immunogenicity of Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigens. Research In Veterinary Science, 95, 123-129. [More Information]
  • Whittington, R., Whittington, A., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Plain, K. (2013). Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium. Journal of Clinical Microbiology, 51(12), 3993-4000. [More Information]
  • Thirunavukkarasu, S., de Silva, K., Whittington, R., Plain, K. (2013). In vivo and in vitro expression pattern of Toll-like receptors in Mycobacterium avium subspecies paratuberculosis infection. Veterinary Immunology and Immunopathology, 156, 20-31. [More Information]
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2012). Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection. Veterinary Immunology and Immunopathology, 148(1-2), 29-47.
  • Plain, K., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Enhancement of the interferon gamma assay to detect paratuberculosis using interleukin-7 and interleukin-12 potentiation. Veterinary Immunology and Immunopathology, 149(1-2), 28-37. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2012). Expression of genes associated with the antigen presentation and processing pathway are consistently regulated in early Mycobacterium avium subsp. paratuberculosis infection. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 35(2), 151-162. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2011). Candidate gene and genome-wide association studies of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep: A review. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 34(3), 197-208. [More Information]
  • Begg, D., de Silva, K., Carter, N., Plain, K., Purdie (Williams), A., Whittington, R. (2011). Does a Th1 over Th2 dominancy really exist in the early stages of Mycobacterium avium subspecies paratuberculosis infections? Immunobiology, 216(7), 840-846. [More Information]
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2011). Indoleamine 2,3-Dioxygenase, Tryptophan Catabolism, and Mycobacterium avium subsp. paratuberculosis: a model for Chronic Mycobacterial Infection. Infection and Immunity, 79(9), 3821-3832. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2011). The interleukin 10 response in ovine Johne's disease. Veterinary Immunology and Immunopathology, 139(1), 10-16. [More Information]
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2010). Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis. Veterinary Microbiology, 141, 301-311.
  • de Silva, K., Begg, D., Carter, N., Taylor, D., Di Fiore, L., Whittington, R. (2010). The early lympocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis  compared to infection status. Immunobiology, 215(1), 12-25.
  • Plain, K., Purdie (Williams), A., Begg, D., de Silva, K., Whittington, R. (2010). Toll-like receptor (TLR)6 and TLR1 differentiation in gene expression studies of Johne's disease. Veterinary Immunology and Immunopathology, 137(1-2), 142-148. [More Information]
  • Begg, D., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D., Jungersen, G., Whittington, R. (2009). Enzyme-linked immunospot: An alternative method for the detection of interferon gamma in Johne's disease. Journal of Veterinary Diagnostic Investigation, 21(2), 187-196.
  • Zhong, L., Di Fiore, L., Taylor, D., Begg, D., de Silva, K., Whittington, R. (2009). Identification of differentially expressed genes in ileum, intestinal lymph node and peripheral blood mononuclear cells of sheep infected with Mycobacterium avium subsp. paratuberculosis using differential display polymerase chain reaction. Veterinary Immunology and Immunopathology, 131(3-4), 177-189. [More Information]
  • Taylor, D., Zhong, L., Begg, D., de Silva, K., Whittington, R. (2008). Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep. Veterinary Immunology and Immunopathology, 124, 132-151.
  • Taylor, D., Thomson, P., de Silva, K., Whittington, R. (2007). Validation of endogenous reference genes for expression profiling of RAW264.7 cells infected with Mycobacterium avium subsp. paratuberculosis by quantitive PCR. Veterinary Immunology and Immunopathology, 115, 43-55.
  • de Silva, K., Daud, A., Deng, J., Jones, S., Gamelli, R., Shankar, R. (2003). Prostaglandin E2 mediates growth arrest in NFS-60 cells by down-regulating interleukin-6 receptor expression. Biochemical Journal, 370(1), 315-321.

Conferences

  • de Silva, K., Begg, D., Purdie (Williams), A., Plain, K., Whittington, R. (2013). Comparison Of The Host Immune Response To Gudair® Vaccination And Map Exposure. International Veterinary Immunology Syposium.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2012). Differential gene expression changes associated with sub-clinical Mycobacterium avium subspecies paratuberculosis infection in cattle: a transcriptomic study. Immunology 2012, usa: N/A.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2012). Early biomarkers of resistance and susceptibility to mycobacterial disease. Immunology 2012, usa: N/A.
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storage time of blood in interferon gamma assays to diagnose paratuberculosis: combination of IL-7 and IL-12 stimulation. 6th International Veterinary Vaccines and Diagnostics Conference, N/A: N/A.
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storage time of blood in interferon gamma assays to disgnose paratuberculosis: combination of II-7 and II-12 stimulation. 4th European Veterinary Immunology Workshop (EVIW), N/A: N/A.
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storgage time of blood in interferon gamma assay to diagnose paratuberculosis: reporting guidelines. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2012). Immune profiles and faecal shedding of Mycobacterium avium paratuberculosis indicate eventual severity of Johne's Disease. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Eckstein, T., Whittington, R. (2012). Macrophage immune responses to mycobacterium avium subsp. paratuberculosis (MAP) and antigens. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Thirunavukkarasu, S., Purdie (Williams), A., Plain, K., de Silva, K., Whittington, R. (2012). Novel gene expression profile in the blood cells of cattle experimentally exposed to Mycobacterium avium subspecies paratuberculosis. IMMUNOLOGY2012™ The 99th AAI Annual Meeting!, USA: N/A.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2012). The role of indoleamine 2,3-dioxygenase (IDO) and tryptophan catabolism in paratuberculosis disease progression. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2012). Transcriptomic analysis of sub clinical mycobacterium avium subsp.paratuberculosis infection in cattle. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Bhardwaj, V., de Silva, K., Dhungyel, O., Whittington, R. (2011). Host Response to Footrot Infection and Vaccination in Sheep. Australian Society for Microbiology Annual Scientific Meeting, N/A: N/A.
  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Whittington, R. (2010). Does a Th1 over Th2 dominance exist in the early stages of Mycobacterium avium subsp. paratuberculosis infections? 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2010). Immune correlates of protection and susceptibility to mycobacterial disease. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Kawaji, S., Plain, K., Whittington, R. (2010). Immune profile and faecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne’s disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K. (2010). Immune profile and fecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne's disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Immunological characteristics of paratuberculosis as a mycobacterial infection. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Mycobacterial infection – lessons from tuberculosis and paratuberculosis. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2010). Role of IDO and tryptophan catabolism in chronic mycobacterial disease pathogenesis. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2009). Development of an experimental infection model for Johne's disease using a lyophilised pure culture seedstock of Mycobacterium avium subspecies paratuberculosis. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Begg, D., Plain, K., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an Experimental Infection Model in Cattle using a Low Passage Seed Stock of MAP. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Plain, K., Begg, D., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an experimental infection protocol in cattle using a low passage cultured strain of MAP. 10th International Colloquium on Paratuberculosis, USA: University of Minnesota.
  • de Silva, K., Begg, D., Whittington, R. (2009). The early IL-10 response in ovine Johne's disease. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Taylor, D., Zhong, L., Di Fiore, L., Begg, D., de Silva, K., Whittington, R. (2008). Analysis of Toll-like receptor gene expression in Mycobacterium paratuberculosis infected sheep. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Browne, S., de Silva, K., Begg, D., Whittington, R., Emery, D. (2008). Apoptosis of mononuclear cells in experimental and natural ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2008). Experimental infection of sheep with M.avium subspecies paratuberculosis: a brief review and introduction to an Australian ovine challenge model. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • de Silva, K., Begg, D., Taylor, D., Di Fiore, L., Whittington, R. (2008). Proliferation of lymphocyte subsets in experimental ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2008). Seeing spots, developing an IFN-gamma ELISPOT assay to detect ovine M. avium subspecies paratuberculosis infection. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Whittington, R. (2008). The IFN-g to antibody switch - does it really happen in Mycobacterium paratuberculosis infections? The 38th Australasian Society for Immunology Annual Conference ASI 2008, Australia: Australasian Society for Immunology.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identificaiton of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M.ptb. ) infected sheep. The 8th International Colloquium on Paratuberculosis, N/A: N/A.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identification of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M. ptb.) infected sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2006). Optimisation of the interferon-gamma assay to maximise responses to M. ptb. antigen in sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2006). Seeing spots, using the ELISPOT assay to detect M. avium subspecies paratuberculosis infection in sheep. Australasian Society for Immunology Conference 2006, Australia: Australasian Society for Immunology.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptosis in sheep exposed to Mycobacterium avium paratuberculosis. Apoptosis and Immunity Conference, Melbourne, Victoria, Australia: Peter MacCallum Cancer Centre.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptotic responses during the pathogenesis of ovice Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • de Silva, K., Begg, D., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2005). Early cellular responses in ovine Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Begg, D., Bosward, K., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). ELISPOT - new methods for detecting IFN-y. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2005). Gene expression and survival of M. paratuberculosis infected macrophages. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., McGregor, H., Whittington, R. (2005). Gene expression signals from the host. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). Manipulation of the Interferon - gamma assay to maximise responses to M. ptb antigen in sheep. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., Gumber, S., de Silva, K., Bower (Goldsmith), K., Di Fiore, L., Begg, D., Whittington, R. (2005). Molecular approaches to studying the host-parasite interaction. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Ovine apoptotic responses to Mycobacterium avium paratuberculosis. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • de Silva, K., Carter, N., Taylor, D., Di Fiore, L., Whittington, R., Emery, D. (2005). Proliferative responses in peripheral blood and lymph node cells in the early stages of ovine Johne's disease. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.

2014

  • Houang, M., Toon, C., Clarkson, A., Sioson, L., de Silva, K., Watson, N., Singh, N., Chou, A., Gill, A. (2014). ALK and ROS1 overexpression is very rare in colorectal adenocarcinoma. Applied Immunohistochemistry and Molecular Morphology, Article in Press.
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Begg, D., Whittington, R., Purdie (Williams), A. (2014). Expression of genes associated with cholesterol and lipid metabolism identified as a novel pathway in the early pathogenesis of Mycobacterium avium subspecies paratuberculosis - infection in cattle. Veterinary Immunology and Immunopathology, 160, 147-157. [More Information]
  • Plain, K., Marsh, I., Waldron, A., Galea, F., Whittington, A., Saunders, V., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2014). High-Throughput Direct Faecal PCR Assay to detect Mycobacterium avium subspecies paratuberculosis in sheep and cattle. Journal of Clinical Microbiology, 52(3), 745-757. [More Information]
  • Gurung, R., Begg, D., Purdie (Williams), A., de Silva, K., Bannantine, J., Whittington, R. (2014). Lymphoproliferative and IFN-y responses to stress-regulated Mycobacterium avium subspecies paratuberculosis recombinant proteins. Clinical and Vaccine Immunology, 21(6), 831-837. [More Information]

2013

  • de Silva, K., Browne, S., Begg, D., Whittington, R., Emery, D. (2013). Apoptosis of lymph node and peripheral blood cells in ovine Johne's disease. Veterinary Immunology and Immunopathology, 156(1-2), 82-90. [More Information]
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2013). Can early host responses to mycobacterial infection predict eventual disease outcomes? Preventive Veterinary Medicine, 112, 203-212. [More Information]
  • Thirunavukkarasu, S., Plain, K., Eckstein, T., de Silva, K., Whittington, R. (2013). Cellular and humoral immunogenicity of Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigens. Research In Veterinary Science, 95, 123-129. [More Information]
  • de Silva, K., Begg, D., Purdie (Williams), A., Plain, K., Whittington, R. (2013). Comparison Of The Host Immune Response To Gudair® Vaccination And Map Exposure. International Veterinary Immunology Syposium.
  • Whittington, R., Whittington, A., Waldron, A., Begg, D., de Silva, K., Purdie (Williams), A., Plain, K. (2013). Development and validation of a liquid medium (M7H9C) for routine culture of Mycobacterium avium subsp. paratuberculosis to replace modified Bactec 12B medium. Journal of Clinical Microbiology, 51(12), 3993-4000. [More Information]
  • Thirunavukkarasu, S., de Silva, K., Whittington, R., Plain, K. (2013). In vivo and in vitro expression pattern of Toll-like receptors in Mycobacterium avium subspecies paratuberculosis infection. Veterinary Immunology and Immunopathology, 156, 20-31. [More Information]

2012

  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2012). Comparative immunological and microbiological aspects of paratuberculosis as a model mycobacterial infection. Veterinary Immunology and Immunopathology, 148(1-2), 29-47.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2012). Differential gene expression changes associated with sub-clinical Mycobacterium avium subspecies paratuberculosis infection in cattle: a transcriptomic study. Immunology 2012, usa: N/A.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Dhand, N., Whittington, R. (2012). Early biomarkers of resistance and susceptibility to mycobacterial disease. Immunology 2012, usa: N/A.
  • Plain, K., Begg, D., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Enhancement of the interferon gamma assay to detect paratuberculosis using interleukin-7 and interleukin-12 potentiation. Veterinary Immunology and Immunopathology, 149(1-2), 28-37. [More Information]
  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2012). Expression of genes associated with the antigen presentation and processing pathway are consistently regulated in early Mycobacterium avium subsp. paratuberculosis infection. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 35(2), 151-162. [More Information]
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storage time of blood in interferon gamma assays to diagnose paratuberculosis: combination of IL-7 and IL-12 stimulation. 6th International Veterinary Vaccines and Diagnostics Conference, N/A: N/A.
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storage time of blood in interferon gamma assays to disgnose paratuberculosis: combination of II-7 and II-12 stimulation. 4th European Veterinary Immunology Workshop (EVIW), N/A: N/A.
  • Begg, D., Plain, K., de Silva, K., Purdie (Williams), A., Whittington, R. (2012). Extension of the storgage time of blood in interferon gamma assay to diagnose paratuberculosis: reporting guidelines. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2012). Immune profiles and faecal shedding of Mycobacterium avium paratuberculosis indicate eventual severity of Johne's Disease. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Thirunavukkarasu, S., Plain, K., de Silva, K., Eckstein, T., Whittington, R. (2012). Macrophage immune responses to mycobacterium avium subsp. paratuberculosis (MAP) and antigens. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Thirunavukkarasu, S., Purdie (Williams), A., Plain, K., de Silva, K., Whittington, R. (2012). Novel gene expression profile in the blood cells of cattle experimentally exposed to Mycobacterium avium subspecies paratuberculosis. IMMUNOLOGY2012™ The 99th AAI Annual Meeting!, USA: N/A.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2012). The role of indoleamine 2,3-dioxygenase (IDO) and tryptophan catabolism in paratuberculosis disease progression. 11th International Colloquium on Paratuberculosis, N/A: N/A.
  • Purdie (Williams), A., Begg, D., de Silva, K., Plain, K., Whittington, R. (2012). Transcriptomic analysis of sub clinical mycobacterium avium subsp.paratuberculosis infection in cattle. 11th International Colloquium on Paratuberculosis, N/A: N/A.

2011

  • Purdie (Williams), A., Plain, K., Begg, D., de Silva, K., Whittington, R. (2011). Candidate gene and genome-wide association studies of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep: A review. Comparative Immunology, Microbiology and Infectious Diseases: the international journal for medical and veterinary researchers and practitioners, 34(3), 197-208. [More Information]
  • Begg, D., de Silva, K., Carter, N., Plain, K., Purdie (Williams), A., Whittington, R. (2011). Does a Th1 over Th2 dominancy really exist in the early stages of Mycobacterium avium subspecies paratuberculosis infections? Immunobiology, 216(7), 840-846. [More Information]
  • Bhardwaj, V., de Silva, K., Dhungyel, O., Whittington, R. (2011). Host Response to Footrot Infection and Vaccination in Sheep. Australian Society for Microbiology Annual Scientific Meeting, N/A: N/A.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2011). Indoleamine 2,3-Dioxygenase, Tryptophan Catabolism, and Mycobacterium avium subsp. paratuberculosis: a model for Chronic Mycobacterial Infection. Infection and Immunity, 79(9), 3821-3832. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2011). The interleukin 10 response in ovine Johne's disease. Veterinary Immunology and Immunopathology, 139(1), 10-16. [More Information]

2010

  • Begg, D., de Silva, K., Plain, K., Purdie (Williams), A., Whittington, R. (2010). Does a Th1 over Th2 dominance exist in the early stages of Mycobacterium avium subsp. paratuberculosis infections? 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2010). Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis. Veterinary Microbiology, 141, 301-311.
  • de Silva, K., Begg, D., Plain, K., Purdie (Williams), A., Kawaji, S., Whittington, R. (2010). Immune correlates of protection and susceptibility to mycobacterial disease. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Kawaji, S., Plain, K., Whittington, R. (2010). Immune profile and faecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne’s disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • de Silva, K. (2010). Immune profile and fecal shedding of Mycobacterium avium paratuberculosis vary with lesion severity in Johne's disease. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Immunological characteristics of paratuberculosis as a mycobacterial infection. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Whittington, R., Begg, D., de Silva, K., Plain, K., Purdie (Williams), A. (2010). Mycobacterial infection – lessons from tuberculosis and paratuberculosis. 9th IVIS International Veterinary Immunology Symposium, Amsterdam: Elsevier.
  • Plain, K., de Silva, K., Earl, J., Begg, D., Purdie (Williams), A., Whittington, R. (2010). Role of IDO and tryptophan catabolism in chronic mycobacterial disease pathogenesis. 41st Australasian Society for Immunology Annual Meeting, N/A: N/A.
  • de Silva, K., Begg, D., Carter, N., Taylor, D., Di Fiore, L., Whittington, R. (2010). The early lympocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis  compared to infection status. Immunobiology, 215(1), 12-25.
  • Plain, K., Purdie (Williams), A., Begg, D., de Silva, K., Whittington, R. (2010). Toll-like receptor (TLR)6 and TLR1 differentiation in gene expression studies of Johne's disease. Veterinary Immunology and Immunopathology, 137(1-2), 142-148. [More Information]

2009

  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Bower (Goldsmith), K., Zhong, L., Kawaji, S., Emery, D., Whittington, R. (2009). Development of an experimental infection model for Johne's disease using a lyophilised pure culture seedstock of Mycobacterium avium subspecies paratuberculosis. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Begg, D., Plain, K., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an Experimental Infection Model in Cattle using a Low Passage Seed Stock of MAP. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.
  • Plain, K., Begg, D., Waldron, A., de Silva, K., Whittington, R. (2009). Development of an experimental infection protocol in cattle using a low passage cultured strain of MAP. 10th International Colloquium on Paratuberculosis, USA: University of Minnesota.
  • Begg, D., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D., Jungersen, G., Whittington, R. (2009). Enzyme-linked immunospot: An alternative method for the detection of interferon gamma in Johne's disease. Journal of Veterinary Diagnostic Investigation, 21(2), 187-196.
  • Zhong, L., Di Fiore, L., Taylor, D., Begg, D., de Silva, K., Whittington, R. (2009). Identification of differentially expressed genes in ileum, intestinal lymph node and peripheral blood mononuclear cells of sheep infected with Mycobacterium avium subsp. paratuberculosis using differential display polymerase chain reaction. Veterinary Immunology and Immunopathology, 131(3-4), 177-189. [More Information]
  • de Silva, K., Begg, D., Whittington, R. (2009). The early IL-10 response in ovine Johne's disease. 10th International Colloquium on Paratuberculosis, Minnesota: University of Minnesota.

2008

  • Taylor, D., Zhong, L., Di Fiore, L., Begg, D., de Silva, K., Whittington, R. (2008). Analysis of Toll-like receptor gene expression in Mycobacterium paratuberculosis infected sheep. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Browne, S., de Silva, K., Begg, D., Whittington, R., Emery, D. (2008). Apoptosis of mononuclear cells in experimental and natural ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2008). Experimental infection of sheep with M.avium subspecies paratuberculosis: a brief review and introduction to an Australian ovine challenge model. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • de Silva, K., Begg, D., Taylor, D., Di Fiore, L., Whittington, R. (2008). Proliferation of lymphocyte subsets in experimental ovine Johne's disease. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2008). Seeing spots, developing an IFN-gamma ELISPOT assay to detect ovine M. avium subspecies paratuberculosis infection. 9th International Colloquium on Paratuberculosis ICP 2007, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Whittington, R. (2008). The IFN-g to antibody switch - does it really happen in Mycobacterium paratuberculosis infections? The 38th Australasian Society for Immunology Annual Conference ASI 2008, Australia: Australasian Society for Immunology.
  • Taylor, D., Zhong, L., Begg, D., de Silva, K., Whittington, R. (2008). Toll-like receptor genes are differentially expressed at the sites of infection during the progression of Johne's disease in outbred sheep. Veterinary Immunology and Immunopathology, 124, 132-151.

2007

  • Taylor, D., Thomson, P., de Silva, K., Whittington, R. (2007). Validation of endogenous reference genes for expression profiling of RAW264.7 cells infected with Mycobacterium avium subsp. paratuberculosis by quantitive PCR. Veterinary Immunology and Immunopathology, 115, 43-55.

2006

  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identificaiton of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M.ptb. ) infected sheep. The 8th International Colloquium on Paratuberculosis, N/A: N/A.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., Begg, D., Emery, D., Whittington, R. (2006). Identification of differentially expressed genes in uninfected and Mycobacterium paratuberculosis (M. ptb.) infected sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2006). Optimisation of the interferon-gamma assay to maximise responses to M. ptb. antigen in sheep. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2006). Seeing spots, using the ELISPOT assay to detect M. avium subspecies paratuberculosis infection in sheep. Australasian Society for Immunology Conference 2006, Australia: Australasian Society for Immunology.

2005

  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptosis in sheep exposed to Mycobacterium avium paratuberculosis. Apoptosis and Immunity Conference, Melbourne, Victoria, Australia: Peter MacCallum Cancer Centre.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Apoptotic responses during the pathogenesis of ovice Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • de Silva, K., Begg, D., Di Fiore, L., Taylor, D., Emery, D., Whittington, R. (2005). Early cellular responses in ovine Johne's disease. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Begg, D., Bosward, K., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). ELISPOT - new methods for detecting IFN-y. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., de Silva, K., Di Fiore, L., Whittington, R. (2005). Gene expression and survival of M. paratuberculosis infected macrophages. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • Di Fiore, L., Taylor, D., de Silva, K., Bosward, K., McGregor, H., Whittington, R. (2005). Gene expression signals from the host. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Bosward, K., Begg, D., de Silva, K., Di Fiore, L., Taylor, D., Whittington, R. (2005). Manipulation of the Interferon - gamma assay to maximise responses to M. ptb antigen in sheep. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Taylor, D., Gumber, S., de Silva, K., Bower (Goldsmith), K., Di Fiore, L., Begg, D., Whittington, R. (2005). Molecular approaches to studying the host-parasite interaction. MLA OJD Harvest Year Conference, North Sydney, N.S.W: Meat & Livestock Australia Ltd.
  • Browne, S., de Silva, K., Whittington, R., Emery, D. (2005). Ovine apoptotic responses to Mycobacterium avium paratuberculosis. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.
  • de Silva, K., Carter, N., Taylor, D., Di Fiore, L., Whittington, R., Emery, D. (2005). Proliferative responses in peripheral blood and lymph node cells in the early stages of ovine Johne's disease. 8th International Colloquium on Paratuberculosis ICP 2005, United States: International Association for Paratuberculosis.

2003

  • de Silva, K., Daud, A., Deng, J., Jones, S., Gamelli, R., Shankar, R. (2003). Prostaglandin E2 mediates growth arrest in NFS-60 cells by down-regulating interleukin-6 receptor expression. Biochemical Journal, 370(1), 315-321.

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