Associate Professor Simon De Graaf

BScAgr(Hons/Medal), PhD, GradCertEducStud(Higher Education)
Associate Professor of Animal Reproduction

B19 - R.M.C. Gunn Building
The University of Sydney

Telephone +61 2 9351 3363
Fax +61 2 9351 3957

Website Twitter
Animal Reproduction Group (ARGUS)
Google Scholar

Research interests

Applied reproduction in domestic and wild animals.

Semen and embryo preservation, artificial insemination and embryo transfer. In vitro fertilisation and embryo culture. Production of pre-sexed offspring by flow cytometric sex-sorting. The role of seminal plasma in sperm function and fertility.

Teaching and supervision

Reproductive biology, reproductive physiology and applied reproduction in units of study within the BScVet (VETS2016; VETS4233; VETS5360) and BAnVetBioSc (ANSC3102; ANSC3104) degrees.

Undergraduate and postgraduate research training in research areas listed below.

Current research students

Project title Research student
Effect of exogenous hormones used for controlled breeding on ovine cervicovaginal mucus and its interaction with ram semen Jessie MADDISON
Identify and apply proteins to improve freezing success and promote fertility of frozen-thawed ram sperm. Taylor PINI

Associations

Association for Applied Animal Andrology (AAAA) Society of Reproductive Biology SRB) International Embryo Transfer Society (IETS) International Congress on Animal Reproduction (ICAR)

International links

France

(Institut National de la Recherche Agronomique (INRA), Nouzilly, France ) Collaboration with Dr Xavier Druart on interaction between sperm, seminal plasma and the female reproductive tract in sheep

Germany

(Department of Biotechnology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Neustadt) Collaboration with Prof Dr Detlef Rath on sperm sexing by flow cytometry

Germany

(Leibniz Institute for Zoo and Wildlife Research, Berlin) Collaboration with Dr Robert Hermes on basic and applied rhino reproduction

Netherlands

(Faculty of Veterinary Medicine, Utrecht University, The Netherlands) Collaboration with Associate Professor Bart Gadella on sperm capacitation and lipidomics in mammalian reproduction

Selected grants

2015

  • Fertility of sexed ram semen; de Graaf S, Leahy T, Rickard J; Australian Wool Innovation Limited/Research Support.
  • Development of oocyte transfer technology and laparoscopic insemination method for creation of a high productivity meat sheep flock; de Graaf S, Leahy T; Ministry of Education and Science of the Republic of Kazakhstan/Research Support.

2014

  • Developing Assisted Reproductive Technologies in Dromedary Camels; de Graaf S; Sultanate of Oman, Royal Court Affairs./Research Support.
  • CLARIOstar Multifunctional Microplate Reader for Shared Used at the Open Access, Multi-User Molecular Biology Core Facility; Huang M, Scolyer R, Fraser S, Sahni S, Speranza T, Owens T, Barrs V, Ju Y, Kovacevic Z, Lu Z, Camp A, Slobedman B, Morris B, Oliver B, Dos Remedios C, Kalinowski D, Fu D, Lai D, Lovicu F, Allbutt H, Triccas J, Burgess J, Kril J, Dixon K, Rendina L, Byrne M, King N, Groundwater P, Williamson P, Ho J, Dong Q, Bao B, Assinder S, McLennan S, Richardson D, Ammit A, Sharland A, Hardikar A, Hong A, Hambly B, Lee C, Murphy C, Goldsbury C, Johnstone D, Lane D, Hibbs D, Joshua D, Huq F, Halliday G, Sutherland G, Zreiqat H, Lok H, Lyons G, Jansson P, Black J, Zhu L, Sunde M, Day M, Naylor M, Buckland M, Murray M, Shafie N, Lay P, Poronnik P, Codd R, Mason R, Ryan R, Christopherson R, de Graaf S; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • Packed to perform: the effects of telomere traits and free radicals on sperm phenotypes, fertilization success, and offspring viability; Olsson M, Tobler M, de Graaf S; Australian Research Council (ARC)/Discovery Projects (DP).
  • ACHIEVING LOW COST (CERVICAL) ARTIFICIAL INSEMINATION WITH FROZEN RAM SEMEN; de Graaf S, Bathgate R; Australian Wool Innovation Limited/Research and Development.

2013

  • Fibre quality: Meeting market requirements; McGregor B, de Graaf S; Australian Wool Innovation Limited/Research and Development.
  • Robotic High Throughput Western Analysis for the Open Access, Multi-User Sydney Cancer Research Core Facility; Richardson D, Scolyer R, Boyer M, Halliday G, Damian D, Christopherson R, Joshua D, Kench J, Hong A, Murray M, Lee C, Kalinowski D, Naylor M, Lay P, Lyons G, Kovacevic Z, Mason R, Dixon K, Chan-Ling T, Hawkins C, Sunde M, Lovejoy D, Owens T, Rendina L, Jansson P, Dos Remedios C, Charles (nee Slaviero) K, Lane D, Witting P, Dong Q, Ammit A, Groundwater P, Assinder S, Bao B, Byrne S, Zhou F, Buckland M, Grewal T, Huq F, Lai D, Codd R, Zhang D, Fu D, de Graaf S, Huang M, Payne R, Slobedman B, Barrs V, Ho J, Williamson P, Murphy C; DVC Research/Equipment Grant.

2011

  • Developing assisted reproductive technologies in camelids, especially the alpaca; de Graaf S, Bathgate R; Rural Industries Research & Development Corporation (RIRDC)/Research Priorities Program.
  • Developing an effective diluent for transport of rabbit semen; Bathgate R, de Graaf S; Rural Industries Research & Development Corporation (RIRDC)/Research Priorities Program.

2010

  • Proteomic analysis of the interaction between sperm and seminal plasma in mammalian species; de Graaf S; The Faculty of Veterinary Science, University of Sydney/Start-up Research Fund.
  • Ruminant Reproduction Symposium - Successful cryopreservation of embryos produced from superovulated ewes inseminated with sex-sorted ram spermatozoa; de Graaf S; The Faculty of Veterinary Science, University of Sydney/Postdoctoral fellow and new academic staff travel funds.

2009

  • In vitro production of pig embryos using sex-sorted spermatozoa; de Graaf S; The Faculty of Veterinary Science, University of Sydney/Start-up Research Fund.

Selected publications

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Book Chapters

  • de Graaf, S. (2010). Reproduction. In D.J. Cottle (Eds.), International Sheep and Wool Handbook, (pp. 189-222). Nottingham: Nottingham University Press.

Journals

  • Pini, T., Leahy, T., de Graaf, S. (2016). Changes to sperm surface carbohydrates following exposure to seminal plasma and freezing. Animal Reproduction Science, 169, 100-100. [More Information]
  • Leahy, T., Rickard, J., Aitken, J., de Graaf, S. (2016). d-penicillamine prevents ram sperm agglutination by reducing the disulphide bonds of a copper-binding sperm protein. Reproduction, 151, 491-500. [More Information]
  • Al-Bulushi, S., Manjunatha, B., Bathgate, R., de Graaf, S. (2016). Effect of different extenders on sperm motion characteristics, viability and acrosome integrity during liquid storage of dromedary camel semen. Animal Reproduction Science, 169, 127-127. [More Information]
  • Rickard, J., Leahy, T., Druart, X., de Graaf, S. (2016). Exposure to seminal plasma reduces the duration that epididymal ram spermatozoa exhibit wave motion. Animal Reproduction Science, 169, 118-118. [More Information]
  • Bernecic, N., Gadella, B., de Graaf, S., Leahy, T. (2016). Isolation of high density lipoproteins in ovine follicular and oviductal fluid. Animal Reproduction Science, 169, 121-122. [More Information]
  • McGregor, B., de Graaf, S., Hatcher, S. (2016). On-farm factors affecting physical quality of Merino wool. 1. Nutrition, reproduction, health and management. Small Ruminant Research, 137, 138-150. [More Information]
  • Leahy, T., Rickard, J., de Graaf, S. (2016). Penicillamine attenuates agglutination of ram spermatozoa in capacitating media through the reduction of disulphide bonds on a sperm binding protein. Animal Reproduction Science, 169, 95-95. [More Information]
  • Leahy, T., Rickard, J., Aitken, J., de Graaf, S. (2016). Penicillamine prevents ram sperm agglutination in media that support capacitation. Reproduction, 151(2), 167-177. [More Information]
  • Soleilhavoup, C., Riou, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P., Reynaud, K., de Graaf, S., Druart, X. (2016). Proteomes of the Female Genital Tract During the Oestrous Cycle. Molecular and Cellular Proteomics, 15(1), 93-108. [More Information]
  • Rickard, J., Schmidt, R., Maddison, J., Bathgate, R., Lynch, G., Druart, X., de Graaf, S. (2016). Variation in seminal plasma alters the ability of ram spermatozoa to survive cryopreservation. Reproduction, Fertility and Development, 28(4), 516-523. [More Information]
  • Stuart, C., Vaughan, J., Kershaw-Young, C., Wilkinson, J., Bathgate, R., de Graaf, S. (2015). Effects of varying doses of b-nerve growth factor on the timing of ovulation, plasma progesterone concentration and corpus luteum size in female alpacas (Vicugna pacos). Reproduction, Fertility and Development, 27(8), 1181-1186. [More Information]
  • Mata-Campuzano, M., Soleilhavoup, C., Tsikis, G., Martinez-Pastor, F., de Graaf, S., Druart, X. (2015). Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration. Animal Reproduction Science, 162, 31-36. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2015). The Effect of Sperm Concentration and Storage Vessel on Quercetin-Supplemented Rabbit Semen During Chilled Storage. Reproduction in Domestic Animals, 50(4), 567-573. [More Information]
  • Rickard, J., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Lynch, G., Druart, X., de Graaf, S. (2015). The identification of proteomic markers of sperm freezing resilience in ram seminal plasma. Journal of Proteomics, 126, 303-311. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2014). Investigation of in vitro parameters and in vivo fertility of rabbit spermatozoa after chilled storage. Animal Reproduction Science, 147(3-4), 135-143. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2014). Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science, 151(3-4), 208-219. [More Information]
  • Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P., Dacheux, J., Guerin, Y., Gatti, J., de Graaf, S., Druart, X. (2014). Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. Journal of Proteomics, 109(23), 245-260. [More Information]
  • Rickard, J., Pini, T., Soleilhavoup, C., Cognie, J., Bathgate, R., Lynch, G., Evans, G., Maxwell, W., Druart, X., de Graaf, S. (2014). Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction, 148, 469-478. [More Information]
  • Bathgate, R., Mace, N., Heasman, K., Evans, G., Maxwell, W., de Graaf, S. (2013). Birth of Kids after Artificial Insemination with Sex-Sorted, Frozen-Thawed Goat Spermatozoa. Reproduction in Domestic Animals, 48(6), 893-898. [More Information]
  • Garratt, M., Bathgate, R., de Graaf, S., Brooks, R. (2013). Copper-zinc superoxide dismutase deficiency impairs sperm motility and in vivo fertility. Reproduction, 146(4), 297-304. [More Information]
  • de Graaf, S., Rickard, J., Maddison, J., Bathgate, R., Druart, X. (2013). Impaired transport of liquid and frozen stored spermatozoa through the cervix. Reproduction in Domestic Animals, 48(1), 63-63. [More Information]
  • Druart, X., Rickard, J., Mactier, S., Kohnke, P., Kershaw-Young, C., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Grupen, C., de Graaf, S., et al (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics, 91, 13-22. [More Information]
  • Druart, X., Rickard, J., Mactier, S., Kohnke, P., Kershaw-Young, C., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Grupen, C., de Graaf, S., et al (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics, 91, 13-22.
  • Rath, D., Barcikowski, S., de Graaf, S., Garrels, W., Grossfeld, R., Klein, S., Knabe, W., Knorr, C., Kues, W., Meyer, W., et al (2013). Sex selection of sperm in farm animals: status report and developmental prospects. Reproduction, 145, R15-R30. [More Information]
  • Leahy, T., de Graaf, S. (2012). Seminal Plasma and its Effect on Ruminant Spermatozoa During Processing. Reproduction in Domestic Animals, 47(Suppl. 4), 207-213. [More Information]
  • Portas, T., Evans, G., de Graaf, S. (2012). The seminiferous cycle of the rhinoceros. Animal Reproduction Science, 136(1-2), 128-132. [More Information]
  • Beilby, K., de Graaf, S., Evans, G., Maxwell, W., Wilkening, S., Wrenzycki, C., Grupen, C. (2011). Quantitative mRNA expression in ovine blastocysts produced from X- and Y-chromosome bearing sperm, both in vitro and in vivo. Theriogenology: an international journal of animal reproduction, 76(3), 471-481. [More Information]
  • Beilby, K., de Graaf, S., Grupen, C. (2010). The Effect of Sperm and Cryoprotectant concentration on the Freezing Success of Sex Sorted ram Sperm for In-Vitro Fertilization. Theriogenology: an international journal of animal reproduction, 74(5), 786-794. [More Information]
  • Behr, B., Rath, D., Mueller, P., Hildebrandt, T., Goeritz, F., Braun, B., Leahy, T., de Graaf, S., Maxwell, W., Hermes, R. (2009). Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis). Theriogenology: an international journal of animal reproduction, 72(3), 353-364. [More Information]
  • Behr, B., Rath, D., Hildebrandt, T., Goeritz, F., Blottner, S., Portas, T., Bryant, B., Sieg, B., Knieriem, A., de Graaf, S., Maxwell, W., et al (2009). Germany/Australia Index of sperm sex sortability in Elephants and Rhinoceros. Reproduction in Domestic Animals, 44(2), 273-277. [More Information]
  • de Graaf, S., Beilby, K., Underwood, S., Evans, G., Maxwell, W. (2009). Sperm sexing in sheep and cattle: The exception and the rule. Theriogenology: an international journal of animal reproduction, 71(1), 89-97. [More Information]
  • de Graaf, S., Leahy, T., Marti, J., Evans, G., Maxwell, W. (2008). Application of Seminal Plasma in Sex-Sorting and Sperm Cryopreservation. Theriogenology: an international journal of animal reproduction, 70, 1360-1363. [More Information]
  • de Graaf, S., Evans, G., Maxwell, W., Cran, D., O'Brien, J. (2007). Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(2), 391-398.
  • de Graaf, S., Beilby, K., O'Brien, J., Osborn, D., Downing, J., Maxwell, W., Evans, G. (2007). Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(3), 550-555.
  • de Graaf, S., Peake, K., Maxwell, W., O'Brien, J., Evans, G. (2007). Influence of supplementing diet with Oleic and Linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livestock Science, 110, 166-173.
  • Maxwell, W., de Graaf, S., El-Hajj Ghaoui, R., Evans, G. (2007). Seminal plasma effects on sperm handling and female fertility. Society of Reproduction and Fertility Supplement, 64, 13-38.
  • de Graaf, S., Evans, G., Maxwell, W., Downing, J., O'Brien, J. (2007). Successful Low Dose Insemination of Flow Cytometrically Sorted Ram Spermatozoa in Sheep. Reproduction in Domestic Animals, 42, 648-653.
  • de Graaf, S., Evans, G., Gillan, L., Guerra, M., Maxwell, W., O'Brien, J. (2007). The influence of antioxidant, cholesterol and seminal plasma on the in vitro quality of sorted and non-sorted ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(2), 217-227.
  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). In vitro characteristics of fresh and frozen-thawed ram spermatozoa after sex sorting and re-freezing. Reproduction, Fertility and Development, 18(8), 867-874.
  • Morton, K., de Graaf, S., Campbell, A., Tomkins, L., Maxwell, W., Evans, G. (2005). Repeat Ovum Pick-up and in vitro Embryo Production from Adult Ewes with and without FSH treatment. Reproduction in Domestic Animals, 440, 422-428.
  • Maxwell, W., Evans, G., Hollinshead, F., Bathgate, R., de Graaf, S., Eriksson, B., Gillan, L., Morton, K., O'Brien, J. (2004). Integration Of Sperm Sexing Technology Into The Art Toolbox. Animal Reproduction Science, 82-83, 79-95. [More Information]

Conferences

  • de Graaf, S., Leahy, T., Vishwanath, R. (2014). Biological and practical lessons associated with the use of sexed semen. Ninth International Symposium on Reproduction in Domestic Ruminants, Leicestershire: Context Publishing.
  • Maddison, J., Rickard, J., Bathgate, R., Druart, X., de Graaf, S. (2014). Changes to ovine cervical mucus induced by the oestrous cycle, oestrus synchronisation and superovulation. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • de Graaf, S., Rickard, J., Pini, T., Maddison, J., Druart, X., Leahy, T. (2014). Emerging Roles of Seminal Plasma in Sperm Function. 9th Biennial Conference of the Association for Applied Animal Andrology, Newcastle: Association for Applied Animal Andrology.
  • Johinke, D., de Graaf, S., Bathgate, R. (2013). European Society for Reproduction in Domestic Animals. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Rickard, J., Maddison, J., Pini, T., Bathgate, R., Evans, G., Maxwell, W., Lynch, G., Druart, X., de Graaf, S. (2013). Exposure to seminal plasma aids the migration of epididymal ram spermatozoa through the cervix. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.
  • de Graaf, S., Rickard, J., Maddison, J., Bathgate, R., Druart, X. (2013). Impaired transport of liquid and frozen stored spermatozoa through the cervix. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Johinke, D., de Graaf, S., Bathgate, R. (2013). In vitro quality of rabbit spermatozoa during chilled storage at 5 or 15 degrees C over 96 h. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Stuart, C., Craig, J., Kershaw-Young, C., de Graaf, S., Bathgate, R. (2013). The effect of egg yolk and glycerol concentrations on the post-thaw survival of papain-reduced alpaca (Vicugna pacos) spermatozoa. Australian Society of Animal Production, Perth: N/A.
  • Stuart, C., Kershaw-Young, C., de Graaf, S., Bathgate, R. (2013). The effect of four semen diluents on the post-thaw survival of papain-reduced alpaca (Vicugna pacos) spermatozoa. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Rickard, J., Bathgate, R., Maddison, J., Schmidt, R., Lynch, G., Druart, X., de Graaf, S. (2013). The effect of seminal plasma from rams with varied freezing resilience on the cryosurvival of ram spermatozoa. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Rickard, J., Kohnke, P., Bathgate, R., Druart, X., Lynch, G., de Graaf, S. (2012). A preliminary assessment of the ram spermatozoa glycoproteome using 2DLC-MS/MS. International Congress on Animal Reproduction, Canada: Reproduction in Domestic Animals.
  • de Graaf, S., Grupen, C., Rickard, J., Bathgate, R., Mactier, S., Kohnke, B., Crossett, B., Maxwell, W., Christopherson, R., Druart, X. (2012). Identification of Membrane Glycoproteins of Ejaculated and Epididymal Boar Spermatozoa Using LC-MS/MS. The 2nd World Congress of Reproductive Biology, N/A: N/A.
  • Gibb, Z., Grupen, C., de Graaf, S. (2012). Is Sex-Sorting a Reality for the Horse Industry? The Australian College of Veterinary Scientists Science Week, N/A: N/A.
  • White, P., Bathgate, R., de Graaf, S. (2012). Sustainable student-led tutorials in Veterinary Physiology. Australian Veterinary Association conference, Australia: Australian Veterinary Association.
  • Johinke, D., de Graaf, S., Bathgate, R. (2012). The effects of various diluents on sperm motility of rabbit semen stored at 5�?�C for up to 96 hours. International Congress on Animal Reproduction, Canada: Reproduction in Domestic Animals.
  • Johinke, D., de Graaf, S., Bathgate, R. (2012). Viability of rabbit spermatozoa stored in different extenders at 5�?�C or 15�?�C for up to 96 hours. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.
  • Beilby, K., Wilkening, S., de Graaf, S., Wrenzycki, C., Grupen, C. (2011). Quantification of Developmentally Important Gene Transcripts Expressed in Embryos Produced In Vivo and In Vitro Using Sex-Sorted and Non-Sorted Ram Spermatozoa. Annual Conference of the International Embryo Transfer Society, melbourne: CSIRO Publishing.
  • Evans, G., de Graaf, S. (2010). Application of sexed sperm in animal breeding and conservation programs. International Strategic Programs for the Conservation of Animal Genetic Resources for Food and Agriculture, Canada: University of Saskatchewan.
  • de Graaf, S., Druart, X. (2010). Interaction of spermatozoa with the Female Reproductive Tract. Society for Reproductive Biology/Australian Endocrine Society, Australia: Australian Society for Reproductive Biology.
  • Beilby, K., Kaurivi, Y., Maxwell, W., Evans, G., de Graaf, S., Grupen, C. (2010). Production of Embryos in Superovulated Ewes Using Frozen-Thawed, Sex-Sorted and Refrozen-Thawed Sperm. 41st Annual Conference of the Society for Reproductive Biology, Australia: CSIRO Publishing.
  • Maxwell, W., de Graaf, S., El-Hajj Ghaoui, R., Evans, G. (2007). Seminal plasma effects on sperm handling and female fertility. In: Reproduction in Domestic Ruminants VI (INVITED REVIEW). 7th International Symposium on Reproduction in Domestic Ruminants, Nottingham, UK: Nottingham University Press.
  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). In vitro differences between sex-sorted ram sperm originating from fresh or frozen-thawed samples. 39th Annual Conference of Physiology and Pathology of Reproduction and 31st Mutual Conference on Veterinary and Human Reproductive Medicine, u/k: Blackwell Synergy.
  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). Invitro differences between sex sorted Ram sperm Originating from Fresh or Frozen/Thawed samples. European Society of Domestic Animal Reproduction, Germany: Blackwell Publishers.
  • de Graaf, S., Leahy, T., Gillan, L., Evans, G., Maxwell, W., O'Brien, J. (2006). Kinematics of sex-sorted ram spermatozoa in response to seminal plasma. 7th International Symposium on Reproduction in Domestic Ruminants, Nottingham, UK: Nottingham University Press.
  • Maxwell, W., Evans, G., Hollinshead, F., Bathgate, R., de Graaf, S., Eriksson, B., Gillan, L., Morton, K., O'Brien, J. (2004). Intergration Of Sperm Sexing Technology Into The Art Toolbox. 15th International Congress on Animal Reproduction ICAR 2004, Netherlands: Elsevier.
  • de Graaf, S., Gillan, L., Evans, G., Maxwell, W., O'Brien, J. (2004). The effect of sheath fluid on the quality of sex-sorted ram spermatozoa. Annual Conference of the International Embryo Transfer Society, melbourne: CSIRO Publishing.

Magazine / Newspaper Articles

  • Leahy, T., de Graaf, S. (2016). The ART of milk production. Australasian Science, 37 (1).

2016

  • Pini, T., Leahy, T., de Graaf, S. (2016). Changes to sperm surface carbohydrates following exposure to seminal plasma and freezing. Animal Reproduction Science, 169, 100-100. [More Information]
  • Leahy, T., Rickard, J., Aitken, J., de Graaf, S. (2016). d-penicillamine prevents ram sperm agglutination by reducing the disulphide bonds of a copper-binding sperm protein. Reproduction, 151, 491-500. [More Information]
  • Al-Bulushi, S., Manjunatha, B., Bathgate, R., de Graaf, S. (2016). Effect of different extenders on sperm motion characteristics, viability and acrosome integrity during liquid storage of dromedary camel semen. Animal Reproduction Science, 169, 127-127. [More Information]
  • Rickard, J., Leahy, T., Druart, X., de Graaf, S. (2016). Exposure to seminal plasma reduces the duration that epididymal ram spermatozoa exhibit wave motion. Animal Reproduction Science, 169, 118-118. [More Information]
  • Bernecic, N., Gadella, B., de Graaf, S., Leahy, T. (2016). Isolation of high density lipoproteins in ovine follicular and oviductal fluid. Animal Reproduction Science, 169, 121-122. [More Information]
  • McGregor, B., de Graaf, S., Hatcher, S. (2016). On-farm factors affecting physical quality of Merino wool. 1. Nutrition, reproduction, health and management. Small Ruminant Research, 137, 138-150. [More Information]
  • Leahy, T., Rickard, J., de Graaf, S. (2016). Penicillamine attenuates agglutination of ram spermatozoa in capacitating media through the reduction of disulphide bonds on a sperm binding protein. Animal Reproduction Science, 169, 95-95. [More Information]
  • Leahy, T., Rickard, J., Aitken, J., de Graaf, S. (2016). Penicillamine prevents ram sperm agglutination in media that support capacitation. Reproduction, 151(2), 167-177. [More Information]
  • Soleilhavoup, C., Riou, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P., Reynaud, K., de Graaf, S., Druart, X. (2016). Proteomes of the Female Genital Tract During the Oestrous Cycle. Molecular and Cellular Proteomics, 15(1), 93-108. [More Information]
  • Leahy, T., de Graaf, S. (2016). The ART of milk production. Australasian Science, 37 (1).
  • Rickard, J., Schmidt, R., Maddison, J., Bathgate, R., Lynch, G., Druart, X., de Graaf, S. (2016). Variation in seminal plasma alters the ability of ram spermatozoa to survive cryopreservation. Reproduction, Fertility and Development, 28(4), 516-523. [More Information]

2015

  • Stuart, C., Vaughan, J., Kershaw-Young, C., Wilkinson, J., Bathgate, R., de Graaf, S. (2015). Effects of varying doses of b-nerve growth factor on the timing of ovulation, plasma progesterone concentration and corpus luteum size in female alpacas (Vicugna pacos). Reproduction, Fertility and Development, 27(8), 1181-1186. [More Information]
  • Mata-Campuzano, M., Soleilhavoup, C., Tsikis, G., Martinez-Pastor, F., de Graaf, S., Druart, X. (2015). Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration. Animal Reproduction Science, 162, 31-36. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2015). The Effect of Sperm Concentration and Storage Vessel on Quercetin-Supplemented Rabbit Semen During Chilled Storage. Reproduction in Domestic Animals, 50(4), 567-573. [More Information]
  • Rickard, J., Leahy, T., Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Lynch, G., Druart, X., de Graaf, S. (2015). The identification of proteomic markers of sperm freezing resilience in ram seminal plasma. Journal of Proteomics, 126, 303-311. [More Information]

2014

  • de Graaf, S., Leahy, T., Vishwanath, R. (2014). Biological and practical lessons associated with the use of sexed semen. Ninth International Symposium on Reproduction in Domestic Ruminants, Leicestershire: Context Publishing.
  • Maddison, J., Rickard, J., Bathgate, R., Druart, X., de Graaf, S. (2014). Changes to ovine cervical mucus induced by the oestrous cycle, oestrus synchronisation and superovulation. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • de Graaf, S., Rickard, J., Pini, T., Maddison, J., Druart, X., Leahy, T. (2014). Emerging Roles of Seminal Plasma in Sperm Function. 9th Biennial Conference of the Association for Applied Animal Andrology, Newcastle: Association for Applied Animal Andrology.
  • Johinke, D., de Graaf, S., Bathgate, R. (2014). Investigation of in vitro parameters and in vivo fertility of rabbit spermatozoa after chilled storage. Animal Reproduction Science, 147(3-4), 135-143. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2014). Quercetin reduces the in vitro production of H2O2 during chilled storage of rabbit spermatozoa. Animal Reproduction Science, 151(3-4), 208-219. [More Information]
  • Soleilhavoup, C., Tsikis, G., Labas, V., Harichaux, G., Kohnke, P., Dacheux, J., Guerin, Y., Gatti, J., de Graaf, S., Druart, X. (2014). Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. Journal of Proteomics, 109(23), 245-260. [More Information]
  • Rickard, J., Pini, T., Soleilhavoup, C., Cognie, J., Bathgate, R., Lynch, G., Evans, G., Maxwell, W., Druart, X., de Graaf, S. (2014). Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa. Reproduction, 148, 469-478. [More Information]

2013

  • Bathgate, R., Mace, N., Heasman, K., Evans, G., Maxwell, W., de Graaf, S. (2013). Birth of Kids after Artificial Insemination with Sex-Sorted, Frozen-Thawed Goat Spermatozoa. Reproduction in Domestic Animals, 48(6), 893-898. [More Information]
  • Garratt, M., Bathgate, R., de Graaf, S., Brooks, R. (2013). Copper-zinc superoxide dismutase deficiency impairs sperm motility and in vivo fertility. Reproduction, 146(4), 297-304. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2013). European Society for Reproduction in Domestic Animals. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Rickard, J., Maddison, J., Pini, T., Bathgate, R., Evans, G., Maxwell, W., Lynch, G., Druart, X., de Graaf, S. (2013). Exposure to seminal plasma aids the migration of epididymal ram spermatozoa through the cervix. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.
  • de Graaf, S., Rickard, J., Maddison, J., Bathgate, R., Druart, X. (2013). Impaired transport of liquid and frozen stored spermatozoa through the cervix. Reproduction in Domestic Animals, 48(1), 63-63. [More Information]
  • de Graaf, S., Rickard, J., Maddison, J., Bathgate, R., Druart, X. (2013). Impaired transport of liquid and frozen stored spermatozoa through the cervix. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Johinke, D., de Graaf, S., Bathgate, R. (2013). In vitro quality of rabbit spermatozoa during chilled storage at 5 or 15 degrees C over 96 h. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Druart, X., Rickard, J., Mactier, S., Kohnke, P., Kershaw-Young, C., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Grupen, C., de Graaf, S., et al (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics, 91, 13-22. [More Information]
  • Druart, X., Rickard, J., Mactier, S., Kohnke, P., Kershaw-Young, C., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Grupen, C., de Graaf, S., et al (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. Journal of Proteomics, 91, 13-22.
  • Rath, D., Barcikowski, S., de Graaf, S., Garrels, W., Grossfeld, R., Klein, S., Knabe, W., Knorr, C., Kues, W., Meyer, W., et al (2013). Sex selection of sperm in farm animals: status report and developmental prospects. Reproduction, 145, R15-R30. [More Information]
  • Stuart, C., Craig, J., Kershaw-Young, C., de Graaf, S., Bathgate, R. (2013). The effect of egg yolk and glycerol concentrations on the post-thaw survival of papain-reduced alpaca (Vicugna pacos) spermatozoa. Australian Society of Animal Production, Perth: N/A.
  • Stuart, C., Kershaw-Young, C., de Graaf, S., Bathgate, R. (2013). The effect of four semen diluents on the post-thaw survival of papain-reduced alpaca (Vicugna pacos) spermatozoa. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.
  • Rickard, J., Bathgate, R., Maddison, J., Schmidt, R., Lynch, G., Druart, X., de Graaf, S. (2013). The effect of seminal plasma from rams with varied freezing resilience on the cryosurvival of ram spermatozoa. European Society for Reproduction in Domestic Animals, Italy: Reproduction in Domestic Animals.

2012

  • Rickard, J., Kohnke, P., Bathgate, R., Druart, X., Lynch, G., de Graaf, S. (2012). A preliminary assessment of the ram spermatozoa glycoproteome using 2DLC-MS/MS. International Congress on Animal Reproduction, Canada: Reproduction in Domestic Animals.
  • de Graaf, S., Grupen, C., Rickard, J., Bathgate, R., Mactier, S., Kohnke, B., Crossett, B., Maxwell, W., Christopherson, R., Druart, X. (2012). Identification of Membrane Glycoproteins of Ejaculated and Epididymal Boar Spermatozoa Using LC-MS/MS. The 2nd World Congress of Reproductive Biology, N/A: N/A.
  • Gibb, Z., Grupen, C., de Graaf, S. (2012). Is Sex-Sorting a Reality for the Horse Industry? The Australian College of Veterinary Scientists Science Week, N/A: N/A.
  • Leahy, T., de Graaf, S. (2012). Seminal Plasma and its Effect on Ruminant Spermatozoa During Processing. Reproduction in Domestic Animals, 47(Suppl. 4), 207-213. [More Information]
  • White, P., Bathgate, R., de Graaf, S. (2012). Sustainable student-led tutorials in Veterinary Physiology. Australian Veterinary Association conference, Australia: Australian Veterinary Association.
  • Johinke, D., de Graaf, S., Bathgate, R. (2012). The effects of various diluents on sperm motility of rabbit semen stored at 5�?�C for up to 96 hours. International Congress on Animal Reproduction, Canada: Reproduction in Domestic Animals.
  • Portas, T., Evans, G., de Graaf, S. (2012). The seminiferous cycle of the rhinoceros. Animal Reproduction Science, 136(1-2), 128-132. [More Information]
  • Johinke, D., de Graaf, S., Bathgate, R. (2012). Viability of rabbit spermatozoa stored in different extenders at 5�?�C or 15�?�C for up to 96 hours. The Annual Conference of the Australian Society for Reproductive Biology, Australia: Australian Society for Reproductive Biology.

2011

  • Beilby, K., Wilkening, S., de Graaf, S., Wrenzycki, C., Grupen, C. (2011). Quantification of Developmentally Important Gene Transcripts Expressed in Embryos Produced In Vivo and In Vitro Using Sex-Sorted and Non-Sorted Ram Spermatozoa. Annual Conference of the International Embryo Transfer Society, melbourne: CSIRO Publishing.
  • Beilby, K., de Graaf, S., Evans, G., Maxwell, W., Wilkening, S., Wrenzycki, C., Grupen, C. (2011). Quantitative mRNA expression in ovine blastocysts produced from X- and Y-chromosome bearing sperm, both in vitro and in vivo. Theriogenology: an international journal of animal reproduction, 76(3), 471-481. [More Information]

2010

  • Evans, G., de Graaf, S. (2010). Application of sexed sperm in animal breeding and conservation programs. International Strategic Programs for the Conservation of Animal Genetic Resources for Food and Agriculture, Canada: University of Saskatchewan.
  • de Graaf, S., Druart, X. (2010). Interaction of spermatozoa with the Female Reproductive Tract. Society for Reproductive Biology/Australian Endocrine Society, Australia: Australian Society for Reproductive Biology.
  • Beilby, K., Kaurivi, Y., Maxwell, W., Evans, G., de Graaf, S., Grupen, C. (2010). Production of Embryos in Superovulated Ewes Using Frozen-Thawed, Sex-Sorted and Refrozen-Thawed Sperm. 41st Annual Conference of the Society for Reproductive Biology, Australia: CSIRO Publishing.
  • de Graaf, S. (2010). Reproduction. In D.J. Cottle (Eds.), International Sheep and Wool Handbook, (pp. 189-222). Nottingham: Nottingham University Press.
  • Beilby, K., de Graaf, S., Grupen, C. (2010). The Effect of Sperm and Cryoprotectant concentration on the Freezing Success of Sex Sorted ram Sperm for In-Vitro Fertilization. Theriogenology: an international journal of animal reproduction, 74(5), 786-794. [More Information]

2009

  • Behr, B., Rath, D., Mueller, P., Hildebrandt, T., Goeritz, F., Braun, B., Leahy, T., de Graaf, S., Maxwell, W., Hermes, R. (2009). Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis). Theriogenology: an international journal of animal reproduction, 72(3), 353-364. [More Information]
  • Behr, B., Rath, D., Hildebrandt, T., Goeritz, F., Blottner, S., Portas, T., Bryant, B., Sieg, B., Knieriem, A., de Graaf, S., Maxwell, W., et al (2009). Germany/Australia Index of sperm sex sortability in Elephants and Rhinoceros. Reproduction in Domestic Animals, 44(2), 273-277. [More Information]
  • de Graaf, S., Beilby, K., Underwood, S., Evans, G., Maxwell, W. (2009). Sperm sexing in sheep and cattle: The exception and the rule. Theriogenology: an international journal of animal reproduction, 71(1), 89-97. [More Information]

2008

  • de Graaf, S., Leahy, T., Marti, J., Evans, G., Maxwell, W. (2008). Application of Seminal Plasma in Sex-Sorting and Sperm Cryopreservation. Theriogenology: an international journal of animal reproduction, 70, 1360-1363. [More Information]

2007

  • de Graaf, S., Evans, G., Maxwell, W., Cran, D., O'Brien, J. (2007). Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(2), 391-398.
  • de Graaf, S., Beilby, K., O'Brien, J., Osborn, D., Downing, J., Maxwell, W., Evans, G. (2007). Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(3), 550-555.
  • de Graaf, S., Peake, K., Maxwell, W., O'Brien, J., Evans, G. (2007). Influence of supplementing diet with Oleic and Linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livestock Science, 110, 166-173.
  • Maxwell, W., de Graaf, S., El-Hajj Ghaoui, R., Evans, G. (2007). Seminal plasma effects on sperm handling and female fertility. Society of Reproduction and Fertility Supplement, 64, 13-38.
  • Maxwell, W., de Graaf, S., El-Hajj Ghaoui, R., Evans, G. (2007). Seminal plasma effects on sperm handling and female fertility. In: Reproduction in Domestic Ruminants VI (INVITED REVIEW). 7th International Symposium on Reproduction in Domestic Ruminants, Nottingham, UK: Nottingham University Press.
  • de Graaf, S., Evans, G., Maxwell, W., Downing, J., O'Brien, J. (2007). Successful Low Dose Insemination of Flow Cytometrically Sorted Ram Spermatozoa in Sheep. Reproduction in Domestic Animals, 42, 648-653.
  • de Graaf, S., Evans, G., Gillan, L., Guerra, M., Maxwell, W., O'Brien, J. (2007). The influence of antioxidant, cholesterol and seminal plasma on the in vitro quality of sorted and non-sorted ram spermatozoa. Theriogenology: an international journal of animal reproduction, 67(2), 217-227.

2006

  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). In vitro characteristics of fresh and frozen-thawed ram spermatozoa after sex sorting and re-freezing. Reproduction, Fertility and Development, 18(8), 867-874.
  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). In vitro differences between sex-sorted ram sperm originating from fresh or frozen-thawed samples. 39th Annual Conference of Physiology and Pathology of Reproduction and 31st Mutual Conference on Veterinary and Human Reproductive Medicine, u/k: Blackwell Synergy.
  • de Graaf, S., Evans, G., Maxwell, W., O'Brien, J. (2006). Invitro differences between sex sorted Ram sperm Originating from Fresh or Frozen/Thawed samples. European Society of Domestic Animal Reproduction, Germany: Blackwell Publishers.
  • de Graaf, S., Leahy, T., Gillan, L., Evans, G., Maxwell, W., O'Brien, J. (2006). Kinematics of sex-sorted ram spermatozoa in response to seminal plasma. 7th International Symposium on Reproduction in Domestic Ruminants, Nottingham, UK: Nottingham University Press.

2005

  • Morton, K., de Graaf, S., Campbell, A., Tomkins, L., Maxwell, W., Evans, G. (2005). Repeat Ovum Pick-up and in vitro Embryo Production from Adult Ewes with and without FSH treatment. Reproduction in Domestic Animals, 440, 422-428.

2004

  • Maxwell, W., Evans, G., Hollinshead, F., Bathgate, R., de Graaf, S., Eriksson, B., Gillan, L., Morton, K., O'Brien, J. (2004). Integration Of Sperm Sexing Technology Into The Art Toolbox. Animal Reproduction Science, 82-83, 79-95. [More Information]
  • Maxwell, W., Evans, G., Hollinshead, F., Bathgate, R., de Graaf, S., Eriksson, B., Gillan, L., Morton, K., O'Brien, J. (2004). Intergration Of Sperm Sexing Technology Into The Art Toolbox. 15th International Congress on Animal Reproduction ICAR 2004, Netherlands: Elsevier.
  • de Graaf, S., Gillan, L., Evans, G., Maxwell, W., O'Brien, J. (2004). The effect of sheath fluid on the quality of sex-sorted ram spermatozoa. Annual Conference of the International Embryo Transfer Society, melbourne: CSIRO Publishing.

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