This unit of study will focus on the high throughput methods for the analysis of gene structure and function (genomics) and the analysis of proteins (proteomics), which are at the forefront of discovery in the biomedical sciences. The course will concentrate on the hierarchy of gene-protein-structure-function through an examination of modern technologies built on the concepts of genomics versus molecular biology, and proteomics versus biochemistry. Technologies to be examined include DNA sequencing, nucleic acid and protein microarrays, two-dimensional gel electrophoresis of proteins, uses of mass spectrometry for high throughput protein identification, isotope tagging for quantitative proteomics, high-performance liquid chromatography, high-throughput functional assays, affinity chromatography and modern methods for database analysis. Particular emphasis will be placed on how these technologies can provide insight into the molecular basis of changes in cellular function under both physiological and pathological conditions as well as how they can be applied to biotechnology for the discovery of biomarkers, diagnostics, and therapeutics. The practical component is designed to complement the lecture course and will provide students with experience in a wide range of techniques used in proteomics and genomics.
Two 1-hour lectures per week and one 3-hour practical per week.
One 2.5-hour exam (theory and theory of prac 70%), in-semester (practical work and assignments 30%)
BMedSc degree students: You must have successfully completed BMED2401 and an additional 12cp from BMED240X before enrolling in this unit.
[12cp from (BCHM2X71 or BCHM2X72 or BCMB2X01 or BCMB2X02 or DATA2002 or ENVX2001 or BIOL2X22 or GEGE2X01 or MBLG2X71 or QBIO2001)] OR [BMED2401 and BMED2405 and 6cp from (BCHM2X71 or BCMB2X02 or MBLG2X71)]Prohibitions