This unit of study is designed to provide a comprehensive coverage of the activity of genes in living organisms, with a focus on eukaryotic and particularly human systems. The lecture component covers the arrangement and structure of genes, how genes are expressed, promoter activity and enhancer action. This leads into discussions on the biochemical basis of differentiation of eukaryotic cells, the molecular basis of imprinting, epigenetics, and the role of RNA in gene expression. Additionally, the course discusses the effects of damage to the genome and mechanisms of DNA repair. The modern techniques for manipulating and analysing macromolecules such as DNA and proteins and their relevance to medical and biotechnological applications are discussed. Techniques such as the generation of gene knockout and transgenic mice are discussed as well as genomic methods of analysing gene expression patterns. Particular emphasis is placed on how modern molecular biology and biochemical methods have led to our current understanding of the structure and functions of genes within the human genome. The practical course is designed to complement the lecture course and will provide students with experience in a wide range of techniques used in molecular biology laboratories. The lecture component of this unit of study is the same as BCHM3071. Qualified students will attend seminars/practical classes in which more sophisticated topics in gene expression and manipulation will be covered.
Two 1-hour lectures per week; two 3-hours practicals per fortnight
One 2.5-hour exam (theory and theory of prac 70%), in-semester (practical work and assignments 30%)
Lewin, B. Genes XI. 11th edition. Jones and Bartlett. 2014.
BMedSc degree students: You must have successfully completed BMED2401 and an additional 12cp from BMED240X before enrolling in this unit.
[An average mark of 75 in 12cp from (BCHM2X71 or BCHM2X72 or BCMB2X01 or BCMB2X02 or MBLG2X71)] OR [BMED2401 and a mark of 75 or above in BMED2405 and a mark of 75 or above in 6cp from (BCHM2X71 or BCMB2X02 or MBLG2X71)]Prohibitions