Magnetic resonance spectroscopy
Magnetic resonance spectroscopic methods measure the emission and absorption of energies of systems within strong magnetic fields. Nuclear Magnetic Resonance (NMR) spectroscopy is one of the most powerful techniques available to probe molecular structure and characterise complex dynamic systems, and utilises the behaviour of atomic nuclei within strong magnetic fields when irradiated with radiofrequency energy. Electron Paramagnetic Resonance (EPR) spectroscopy is analogous to NMR, but probes the response of electron spins rather than nuclear spins, and is especially useful when investigating systems that contain free radicals or are paramagnetic. Both techniques are non-destructive and can be applied to complex systems with as little as nanogram amounts of material.
What do you want to study?
The Magnetic Resonance node operates many instruments with different capabilities, optimised for different types of samples, environmental conditions and experiments. If you are not sure which instrument you need, download our brief guide to Sydney Analytical's magnetic resonance capabilities and their applications.
Magnetic resonance capabilities
This flagship high-field, narrow-bore, 3 channel NMR spectrometer is equipped with a high-resolution cryogenic triple nucleus probehead (H/C/N) providing significant signal-to-noise advantages over room temperature probes. The TCI probe can handle standard 3mm and 5mm tubes, as well as shaped tubes for high salt content samples. This spectrometer is optimised for the study of complex biomolecular systems, and provides capability for small molecule characterisation where only very limited amounts of samples are available.
A narrow-bore, 3-channel NMR spectrometer equipped with a high-resolution cryogenic triple nucleus probehead (H/C/N). Equipped with a high-throughput autosampler with individual rack temperature control, this system is ideal for metabolomic studies and fragment-based drug development, and supports commonly published metabolomic protocols. It is also ideally suited for the study of biomolecular systems, routine molecular and structural characterisation and drug screening studies
A standard bore 3-channel probe with multinuclear capabilities (15N-19F/H/F) providing a popular choice for 13C detection with simultaneous 1H and 19F decoupling. This instrument is optimised for high-throughput 24/7 walk-up operation and is supported by a 600-position autosampler. It has controlled variable temperature capabilities (180K to 350K) and is ideal for routine small-medium molecule characterisation, structure elucidation and sample monitoring.
A narrow-bore, 3-channel NMR spectrometer running a 5mm multinuclear probehead and a 1.7mm microprobe for improved sensitivity. This instrument is supported by a high-throughput autosampler, has controlled variable temperature capacity between 180K and 350K and is utilised for small -medium molecule characterisation and structure elucidation, and is optimised for quantitative sample measurements to provide excellent screening for sample purity and QC/QA applications.
A narrow-bore, 3-channel NMR spectrometer running a 5mm multinuclear probehead and several triple nucleus probeheads that allow the investigation of specific nuclear combinations. This instrument is supported by a high-throughput autosampler and has controlled variable temperature capacity between 180K and 370K. It is utilised for small -medium molecule characterisation and structure elucidation, investigating molecular dynamics and ligand binding studies. Commonly observed heteronuclei include 2H, 19F, 11B, 13C, 15N, 17O, 19F, 29Si, 31P, 77Se, 119Sn and 195Pt.
The 400-MHz wide-bore NMR spectrometer is a multipurpose instrument supporting all common sample types and forms. It is equipped with 4mm and 1.9mm broadband CP-MAS solids probes, a 4mm X/H CP-MAS probe with extended temperature capabilities (150K to 470K), a high-field gradient (1000 G/cm) diffusion probe, a mini 0.5 micro-imaging system, and several solution probes including 19F, broadband, dual and triple-resonance (H/C/N) probes. Depending on the probe, this system can handle samples in 5mm and 10mm tubes.
This 2-channel solution state instrument equipped with a 5mm multinuclear probehead (19F-15N) provides 24/7 walk up access to routine multinuclear acquisition, and is supported by a 24-sample autosampler, and variable temperature capability within the range of 180K and 350K.
This modern benchtop EPR instrument has a field range of 0 to 6.5kG and delivers excellent sensitivity and stability for a range of research applications. It is equipped with a variable temperature accessory that allows operation in the range of 100K to 425K.