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vibrational spectroscopy machine
Infrastructure_

Vibrational Spectroscopy

Capabilities for point-spectra collection and chemical mapping
The Vibrational Spectroscopy facility at Sydney Analytical offers a range of specialised instruments and sampling accessories for use in simple point spectroscopy or sophisticated chemical/biological mapping and imaging.
infrared spectroscopy machine with person and tweezers

Infrared spectroscopy

In infrared spectroscopy the sample is irradiated with polychromatic light and a photon of light is absorbed when the frequency (energy) of the absorbed light matches the energy required for a particular bond to vibrate within the sample. In order for a vibration to be infrared active the molecular dipole moment must change during the vibration.

The microtiter plate extension (HTS-XT module) enables high throughput screening of samples in transmission mode using standard microplate formats. Around 1 - 20 mL of the liquid sample is placed onto a single position of a 96-well silicon microtiter plate and dried prior to insertion into the spectrometer. A Twister Microplate Handler enables automatic loading and unloading of up to 20 microtiter plates for high-throughput analysis.


This instrument is also equipped with a Bruker BioATR II Unit that is specifically designed for the investigation of temperature-induced unfolding, refolding and denaturation processes of proteins in aqueous media using only 10-20 µL of sample. A programmable thermostat can produce temperatures from 5 to 95°C and can be set up for automatic ramping. The option for flow-through analysis is also available.

The Bruker Tensor 27 spectrometer is equipped to collect spectra over the MIR spectral range (7000 - 600 cm-1). It is coupled to the Hyperion 3000 microscope which has an MCT (Mercury Cadmium Telluride) and FPA (Focal Plane Array) detector allowing collection of spectra in single point, mapping or imaging modes.

Mapping involves the collection of spectra from specific, user-defined regions of interest using points, lines or grids, while imaging is the simultaneous collection of spectra using a 64 x64 FPA detector with each of the 4096 pixels in the array representing a spectrum. 2D/3D false colour maps are generated by plotting the intensity/area/peak-ratio of a characteristic peak(s) vs. the spatial x, y co-ordinates to obtain a visual distribution of one or more characteristic functional groups.

Specialised objectives are available for attenuated total reflectance (ATR) and grazing angle (GA) experiments and a live cell incubator accessory enables real-time analysis of changes in live cells under culture conditions.

This is a high-end research grade FTIR instrument equipped to collect spectra over the NIR (15500 – 4000 cm-1), MIR (12000 – 850 cm-1) and FIR (680 – 50 cm-1) spectral ranges using an evacuated optics bench. A vacuum pump allows for evacuation of the optic bench to significantly reduce the infrared active atmospheric water and carbon dioxide absorptions.

Other advantages that the vacuum system offers include the following:

  • weak spectral features of some samples are not masked by moisture absorptions
  • no problems are caused by the fluctuation of a dry air purge supply.

Sample accessories can be used with this spectrometer to enable analysis using the following method:

  • diffuse reflectance – good for non-reflective materials (highly opaque or weakly absorbing) and irregular surfaces or coatings.
  • specular reflectance – used to study film thickness, material reflectivity, and the uniformity and homogeneity of surface coatings.
  • attenuated total reflectance (ATR) – used for materials that are strong absorbers and can provide information about the surface properties of a material.
  • photoacoustic – suitable for solid, semi-solid and liquid samples as well as dark, highly absorbing samples.

Available mid-2018

This instrument provides morphological, mechanical property and IR spectral information on materials, surfaces and biomaterials (cells, tissues, extracellular vesicles, etc) at resolutions of less than 10 nm (for ideal samples). It is an AFM based instrument where the spatial resolution is defined by the tip of the AFM probe. It has a variety of AFM modes to provide morphological, stiffness and hardness information for with complementary IR spectral information can be obtained. In Neasom mode it has a spatial resolution of less than 10 nm and a single spectrum can be collected at a single point or fast scanning can be conducted by integrating just a single band. The depth of sampling of this technique is ~20 nm. It also has photothermal expansion spectroscopy that produces IR spectra from further into the sample, but with less spatial resolution.

This instrument is designed for the analysis of samples in the near-infrared (NIR) region (12500 – 3600 cm-1). It has high sensitivity and stability due in part to the permanently aligned RockSolidTM interferometer. A wide array of sampling methods makes it suitable for the analysis of liquids, solids, powders, pastes and tablets. The fibre optic probe attachment even allows samples to be measured directly without transferring to a sample holder. Transmission measurements of multiple liquid vials or solid tablets can also be carried out using the automated sample wheel.

A stand alone, fully automated, all-in-one microscope and FTIR spectrometer designed to be extremely easy to use while still providing high quality spectra. The instrument is fitted with a ×8 objective capable of measurements in reflectance, transmission and attenuated total reflectance (ATR) while a digital zoom allows visible light images to be collect at up to ×32 magnification. The numerical aperture of the objective is automatically changed when switching between IR and visible modes to provide high depth of field visible images while maintaining high sensitivity for IR analysis. The micro-ATR crystal has a 100 µm diameter and is fitted with an integrated pressure control to ensure consistent application of pressure by the crystal on the sample.


Raman spectroscopy machine

Raman spectroscopy

In Raman spectroscopy the sample is irradiated with monochromatic light and the photons are either inelastically or elastically scattered. The inelastically scattered light, known as Raman scatter, has lost (Stokes) or gained (Anti–Stokes) energy during this interaction and the emitted photon contains information about the molecular structure of the sample. The elastically scattered light has the same energy as the incident laser light and is called Rayleigh scatter. Modern Raman instruments are designed to filter out the Rayleigh light as only one in every million photons will be Raman scattered. There is one other requirement for a vibration to be Raman active – when the molecule vibrates there must be a change in polarisability i.e., a change in the shape, size or orientation of the electron cloud that surrounds the molecules.

The microscope is a compact automated instrument used for a wide range of experiments from simple point spectroscopy to more sophisticated experiments. Multiple lasers offer excitation lines ranging from the visible to the near infrared (488, 514, 633, 785 and 830 nm) and provide the flexibility to offer the most suitable configuration for a researchers' experimental requirements.

The system is integrated with an atomic force microscope (AFM) to allow for correlated micro- and nano-scale property mapping using both AFM and tip-enhanced Raman spectroscopy (TERS).

Another feature of this system is the ability to map samples. Mapping is a specialised technique used to identify components and visualise their distribution within a sample. There are a number of different mapping modes available including: point-by-point, StreamLine™, StreamLine HR™, Surface and 3D Volume mapping.

A unique state-of-the-art system comprised of two separate Raman spectrometers that operate independently. Five lasers are available the cover a broad range of excitation lines from the deep UV to the NIR (266, 355, 532, 785 and 1064 nm). The Renishaw InVia Qontor confocal spectrometer has an enclosed, upright microscope and features LiveTrackTM focus tracking technology that enables mapping of rough, smooth, flat and curved surfaces by automatically maintaining optimum focus during data collection. For large samples a flexible sampling arm (FSA) can be mounted into the microscope turret in place of an objective with both single point analysis and mapping available.

The second instrument comprises a Renishaw InVia spectrometer with an inverted microscope which allows Raman spectra to be collected from below the sample (rather than from above). This enables data to be easily acquired from liquid samples, e.g. fluids within microplates, petri dishes and live cells in media.

Both instruments now feature the Rapide mapping function which allow samples to be mapped at a fraction of the time usually required for normal StreamLine™, StreamLineHR™ and 3D Volume mapping.

This instrument uses a 1W Nd:YAG air-cooled laser to deliver an excitation wavelength of 1064 nm. The NIR excitation is particularly useful for the analysis of samples that fluoresce when excited with other high-energy visible excitation lines.

A high throughput module can be configured for experiments using either 90 or 180° sampling geometries while fibre optic coupling to a RAMANSCOPE III microscope allows for sample analysis at a microscopic scale. The microscope is equipped with a motorised stage for mapping and offers 8 µm spatial resolution. High throughput optics and Germanium diode detector offers ultra-low signal detection with minimal noise assuring excellent sensitivity. The real-time spectrum display permits software control of the analysis conditions, including optimization of laser power and the sample position.


portable instrument held by woman

Portable instruments

Our range of portable analytical instrumentation allows on-site analyses in situations where sample location, size or fragility might make it unfeasible to carry out laboratory-based investigations. Our technical staff are also available to conduct and supervise this type of field research, and experienced users may request extended and unsupervised use of this instrumentation.   

The ALPHA is a compact, easy to use and portable FTIR spectrometer, with wireless communication, over 8 hours battery operation and a transport case allowing for use in the field, in the VSCF or other locations/laboratories as needed. Re-alignment is not necessary after transporting the instrument. It is insensitive to vibrations allowing the spectrometer to be placed anywhere for use, including in fumehoods.

There are two easily exchangeable sampling modules; the Platinum ATR single reflection diamond for solid and liquid samples and the External Reflection Module (ERM) for non-destructive, contactless analysis of larger samples such as coated metal, paper, textile fabrics, mural paintings or artwork. An integrated video camera in the ERM provides viewing of the sample area.

The MicroNIRTM OnSite is a rugged and durable handheld spectrometer designed for data collection in the field. It provides non-destructive analysis of a wide range of samples with rapid collection times enabling high throughput. The spectrometer is operated through a tablet or laptop via a USB cable and the obtained spectra can be directly exported as a data matrix for multivariate analysis in The UnscramblerTM.

A reliable high performance handheld analyser for sample identification using Raman spectroscopy that an even be used on dark, fluorescing and weakly scattering samples. This instrument uses patented Sequentially Shifted Excitation (SSETM) technology to mitigate fluorescence issues often encountered with Raman analysis. There are two interchangeable sampling heads available for measuring solid and liquid samples, making it ideal for rapid on-site identification and comparison with no sample preparation. Data can be stored on the spectrometer or transferred wirelessly to a PC for more in-depth analyses and data processing.

This is a sensitive, portable, reliable, compact and easy to use Raman spectrometer designed for laboratory or field analysis of samples. It comes complete with a robust carry case, laptop computer, optical fibre coupled laser probe and can be run on mains power or via the included rechargeable battery. The system is ideal for demanding on-site Raman identification, chemical process monitoring in the lab, the direct, non-destructive analysis of large samples and for any research requiring portability and high sensitivity in a Raman system.


X-ray and UV photoelectron spectroscopy

Photoelectron (or photoemission) spectroscopy measures the binding energies in a substance, which are characteristic of the chemical structure and molecular bonding. Using either x-ray (XPS) or ultraviolet light (UPS), this is a very sensitive technique for measuring the nature of surfaces.

The Thermo Fisher Scientific K-Alpha+ instrument for x-ray Photoelectron Spectroscopy and Ultraviolet Photoelectron Spectrscopy (XPS/UPS) will be located in the Sydney Nanoscience Hub. This instrument provides information on elemental composition (XPS), oxidation states (XPS) and electronic states (UPS). It has a scanning mode with 30 micron x,y spatial resolution and depth profiling with about 1 nm spatial resolution. It has a transfer module to enable surfaces and devices to be transferred from cleanrooms or PC labs to the instrument without exposure to surface contamination. This instrument has many applications in materials research, surface science, catalysis and biomaterials.