Characterising changes in a proteome of islets and islet cell types during progression to type 2 diabetes

Summary

Characterizing changes in a proteome through pathogenesis of diseases can provide unparalleled insights into the mechanisms underpinning disease. In this project, using flow cytometry and liquid chromatography mass spectrometry/mass spectrometry we aim to characterise the deep proteome of mouse and human whole islets and isolated individual islet cell types at various stages of T2D progression.

Supervisor(s)

Dr Melkam Kebede

Research Location

School of Life and Environmental Sciences

Program Type

Masters/PHD

Synopsis

In this project, using flow cytometry and liquid chromatography mass spectrometry/mass spectrometry we aim to characterise the deep proteome of both whole islets and individual islet cell types; alpha, beta, gamma, delta and epsilon from mice and humans at variable states of health and disease. By characterising the proteomes of the individual islet cell types, we can observe the communication between the islet cells, the regulation within islets and how this ultimately impacts on the regulation of blood glucose in the whole body. This can be further used to complement whole islet proteomics data of different stages of disease and attribute changes in the proteins specific to a cell type, furthering our understanding of how the cells within islets communicate.

Additional Information

HDR Inherent Requirements

In addition to the academic requirements set out in the Science Postgraduate Handbook, you may be required to satisfy a number of inherent requirements to complete this degree. Example of inherent requirement may include:

- Confidential disclosure and registration of a disability that may hinder your performance in your degree;
- Confidential disclosure of a pre-existing or current medical condition that may hinder your performance in your degree (e.g. heart disease, pace-maker, significant immune suppression, diabetes, vertigo, etc.);
- Ability to perform independently and/or with minimal supervision;
- Ability to undertake certain physical tasks (e.g. heavy lifting);
- Ability to undertake observatory, sensory and communication tasks;
- Ability to spend time at remote sites (e.g. One Tree Island, Narrabri and Camden);
- Ability to work in confined spaces or at heights;
- Ability to operate heavy machinery (e.g. farming equipment);
- Hold or acquire an Australian driver’s licence;
- Hold a current scuba diving license;
- Hold a current Working with Children Check;
- Meet initial and ongoing immunisation requirements (e.g. Q-Fever, Vaccinia virus, Hepatitis, etc.)

You must consult with your nominated supervisor regarding any identified inherent requirements before completing your application.

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Keywords

islet, endocrine cells, beta-cell failure, Type 2 diabetes, deep proteome, alpha cells, Flow cytometry, mass spectrometry, islet cell types, type 2 diabetes progression, Insulin resistance, beta-cell compensation, human islets and pre-diabetes

Opportunity ID

The opportunity ID for this research opportunity is: 2488

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