Characterising insulin secretory granules as a function of age

Summary

In β-cells, insulin is stored in secretory granules (SGs). Upon stimulation, these SGs deliver insulin to the bloodstream. It is known that SGs exist in two functionally distinct pools; and one pool is preferentially secreted upon stimulation. How a cell can differentiate the two SG pools is unclear. In this project we use unbiased approaches to determine the molecular differences between these SG pools.

Supervisor(s)

Dr Melkam Kebede

Research Location

School of Life and Environmental Sciences

Program Type

Masters/PHD

Synopsis

Our aim is to investigate the cause of reduced insulin secretion in type 2 diabetes. In beta-cells, insulin is stored in secretory granules (SGs). Upon stimulation, these SGs deliver insulin to the bloodstream. It is known that SGs exist in two functionally distinct pools; and one pool is preferentially secreted upon stimulation. How a cell can differentiate the two SG pools is unclear. Here we will use unbiased approaches to determine the molecular differences between these SG pools.

Additional Information

HDR Inherent Requirements

In addition to the academic requirements set out in the Science Postgraduate Handbook, you may be required to satisfy a number of inherent requirements to complete this degree. Example of inherent requirement may include:

- Confidential disclosure and registration of a disability that may hinder your performance in your degree;
- Confidential disclosure of a pre-existing or current medical condition that may hinder your performance in your degree (e.g. heart disease, pace-maker, significant immune suppression, diabetes, vertigo, etc.);
- Ability to perform independently and/or with minimal supervision;
- Ability to undertake certain physical tasks (e.g. heavy lifting);
- Ability to undertake observatory, sensory and communication tasks;
- Ability to spend time at remote sites (e.g. One Tree Island, Narrabri and Camden);
- Ability to work in confined spaces or at heights;
- Ability to operate heavy machinery (e.g. farming equipment);
- Hold or acquire an Australian driver’s licence;
- Hold a current scuba diving license;
- Hold a current Working with Children Check;
- Meet initial and ongoing immunisation requirements (e.g. Q-Fever, Vaccinia virus, Hepatitis, etc.)

You must consult with your nominated supervisor regarding any identified inherent requirements before completing your application.

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Keywords

islet, insulin secretion, insulin granules, beta-cell failure, Type 2 diabetes, Flow cytometry, mass spectrometry, Insulin resistance, beta-cell compensation, Electron microscopy, human islets and pre-diabetes

Opportunity ID

The opportunity ID for this research opportunity is: 2489

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