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Crosstalk of noncoding RNA regulatory network with epigenetic pathways determines the fate of stem cells and this discovery paves the way for developing novel RNA-based therapeutics to effectively destroy cancer stem cells.
North Shore - Kolling Institute of Medical Research
The recent discovery of non-coding RNAs (ncRNAs) has dramatically altered our view of gene regulation in cancer. MicroRNAs (miRNAs) are a class of ncRNAs that function to regulate gene expression at the transcriptional and post-transcriptional level, playing a pivotal role in cancer progression and metastasis. Using an integrated miRNA-mRNA expression profiling analysis, we have documented a miRNA regulatory network, whose downregulation is associated with the aggressive phenotype of cancer (Haematologica 2019).
This study will investigate how a crosstalk between miRNA regulatory network and epigenetic pathways determines the fate of stem cells and this will pave the way for developing novel RNA-based therapeutics in effectively destroying malignant stem cells.
Technologies to be used: Single cell omics analysis (transcriptomics, proteomics and epigenomics), cell-based assays, in vitro drug response assays, molecular and cell biology, gene and protein expression, immunofluorescence, gene editing, chromatin immunoprecipitation sequencing (ChIP-seq), flow cytometry, patient-derived xenograft mouse models, in vivo preclinical drug testing, stem cell technologies, nanotechology etc.
The opportunity ID for this research opportunity is 3157