Protein structure, function and engineering lab Research methods used in the lab
Home

Research Interests

Structure Gallery

Publications

Research Methods

Current lab members

Past lab members

Excursions/photos

Contact/find us

Join the lab

Internal

BLOG/news



Random photo

In the course or our research, we use a wide range of experimental methods. Our philisophy is very much one of finding the right method to answer the questions we are interested in. Most of the methods that we use routinely are listed below.
  • Molecular biology
  • Protein expression and purification (from bacterial, mammalian and insect hosts)
  • Yeast two-hybrid
  • Mammalian and insect cell culture
  • GST-pulldowns
  • Gel shift assays (DNA + RNA)
  • SELEX (at least in the old days...)
  • CRISPR/Cas9-based genome engineering
  • Solution NMR spectroscopy
  • X-ray crystallography
  • Single-particle electron microscopy
  • Circular dichroism spectropolarimetry
  • Analytical ultracentrifugation
  • Surface plasmon resonance
  • Mass spectrometry (including quantitative approaches and crosslinking-MS)
  • Isothermal titration calorimetry
  • Differential scanning calorimetry
  • Microscale thermophoresis



Lastest update: "Lab members page", on 31st Aug 2019.


Flag Counter
0.007640s
© W. Yung 2002
SoLES
USyd