Dr Lake-ee Quek


Research interests

Metabolomics, cancer metabolism, computational biology

Selected grants

2018

  • Redefining insulin resistance as a metabolic defect beyond glucose transport; Krycer J, James D, Fazakerley D, Healy (Nelson) M, Quek L; Diabetes Australia/Diabetes Research Grant Program.
  • Chicken and the egg: how does metabolism fine-tune signal transduction?; Krycer J, Fazakerley D, Quek L, O'Sullivan J; Charles Perkins Centre/Early-Mid Career Researcher Seed Funding grant.

2016

  • Targeting Glutamine metabolism to starve triple-negative breast cancer; Holst J, Beith J, Hoy A, Quek L; University of Sydney - Sydney Catalyst Translational Cancer Research/Pilot and Seed Funding.

Selected publications

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Book Chapters

  • Quek, L., Nielsen, L. (2014). Customization of 13C-MFA Strategy According to Cell Culture System. In Jens O. Kromer, Lars K. Nielsen, Lars M. Blank (Eds.), Methods in Molecular Biology: Metabolic Flux Analysis - Methods and Protocols, (pp. 81-90). New York: Humana Press. [More Information]
  • Quek, L., Nielsen, L. (2014). Steady-State 13C Fluxomics Using OpenFLUX. In Jens O. Kromer, Lars K. Nielsen, Lars M. Blank (Eds.), Methods in Molecular Biology: Metabolic Flux Analysis - Methods and Protocols, (pp. 209-224). New York: Humana Press. [More Information]
  • Quek, L., Nielsen, L. (2010). Metabolic Engineering of Mammalian Cells. In Christina D. Smolke (Eds.), The Metabolic Pathway Engineering Handbook: Fundamentals, (pp. 26-1-26-14). Florida, USA: CRC Press.

Journals

  • Small, L., Brandon, A., Quek, L., Krycer, J., James, D., Turner, N., Cooney, G. (2018). Acute activation of pyruvate dehydrogenase increases glucose oxidation in muscle without changing glucose uptake. American Journal of Physiology - Endocrinology and Metabolism, 315(2), E258-E266. [More Information]
  • Van Geldermalsen, M., Quek, L., Turner, N., Freidman, N., Pang, A., Guan, Y., Krycer, J., Ryan, R., Wang, Q., Holst, J. (2018). Benzylserine inhibits breast cancer cell growth by disrupting intracellular amino acid homeostasis and triggering amino acid response pathways. BMC Cancer, 18(1), 689 - 1-689 - 14. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Saac, P., Palfreymana, R., Nielsen, L. (2018). From reconstruction to C4 metabolic engineering: A case study for overproduction of polyhydroxybutyrate in bioenergy grasses. Plant Science, 273, 50-60. [More Information]
  • Bakshi, I., Suryana, E., Small, L., Quek, L., Brandon, A., Turner, N., Cooney, G. (2018). Fructose bisphosphatase 2 overexpression increases glucose uptake in skeletal muscle. Journal of Endocrinology, 237(2), 101-111. [More Information]
  • Buchsteiner, M., Quek, L., Gray, P., Nielsen, L. (2018). Improving culture performance and antibody production in CHO cell culture processes by reducing the Warburg effect. Biotechnology and Bioengineering, 115(9), 2315-2327. [More Information]
  • Bentley, N., Fiveash, C., Osborne, B., Quek, L., Ogura, M., Inagaki, N., Cooney, G., Polly, P., Montgomery, M., Turner, N. (2018). Protein hypoacylation induced by Sirt5 overexpression has minimal metabolic effect in mice. Biochemical and Biophysical Research Communications, 503(3), 1349-1355. [More Information]
  • Krycer, J., Yugi, K., Hirayama, A., Fazakerley, D., Quek, L., Scalzo, R., Ohno, S., Hodson, M., Ikeda, S., Shoji, F., Domanova, W., Parker, B., Healy (Nelson), M., Humphrey, S., Cooney, G., James, D., et al (2017). Dynamic Metabolomics Reveals that Insulin Primes the Adipocyte for Glucose Metabolism. Cell Reports, 21(12), 3536-3547. [More Information]
  • Quek, L., Liu, M., Joshi, S., Turner, N. (2016). Fast exchange fluxes around the pyruvate node: a leaky cell model to explain the gain and loss of unlabelled and labelled metabolites in a tracer experiment. Cancer and Metabolism, 4, 1-14. [More Information]
  • Couttas, T., Kain, N., Suchowerska, A., Quek, L., Turner, N., Fath, T., Garner, B., Don, A. (2016). Loss of ceramide synthase 2 activity, necessary for myelin biosynthesis, precedes tau pathology in the cortical pathogenesis of Alzheimer's disease. Neurobiology of Aging, 43(July), 89-100. [More Information]
  • Marcellin, E., Behrendorff, J., Nagaraju, S., DeTissera, S., Segovia, S., Palfreyman, R., Daniell, J., Licona-Cassani, C., Quek, L., Speight, R., et al (2016). Low carbon fuels and commodity chemicals from waste gases-systematic approach to understand energy metabolism in a model acetogen. Green Chemistry, 18(10), 3020-3028. [More Information]
  • Swainston, N., Smallbone, K., Hefzi, H., Dobson, P., Brewer, J., Hanscho, M., Zielinski, D., Ang, K., Gardiner, N., Gutierrez, J., et al (2016). Recon 2.2: from reconstruction to model of human metabolism. Metabolomics, 12(7), 1-7. [More Information]
  • De Oliveira Dal'Molin, C., Quek, L., Saa, P., Nielsen, L. (2015). A multi-tissue genome-scale metabolic modeling framework for the analysis of whole plant systems. Frontiers in Plant Science, 6, 1-12. [More Information]
  • Martinez, V., Buchsteiner, M., Gray, P., Nielsen, L., Quek, L. (2015). Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism. Metabolic Engineering Communications, 2, 46-57. [More Information]
  • Quek, L., Nielsen, L. (2014). A depth-first search algorithm to compute elementary flux modes by linear programming. BMC Systems Biology, 8(1), 1-10. [More Information]
  • Arifin, Y., Archer, C., Lim, S., Quek, L., Sugiarto, H., Marcellin, E., Vickers, C., Kromer, J., Nielsen, L. (2014). Escherichia coli W shows fast, highly oxidative sucrose metabolism and low acetate formation. Applied Microbiology and Biotechnology, 98(21), 9033-9044. [More Information]
  • Turner, J., Quek, L., Titmarsh, D., Kromer, J., Kao, L., Nielsen, L., Wolvetang, E., Cooper-White, J. (2014). Metabolic Profiling and Flux Analysis of MEL-2 Human Embryonic Stem Cells during Exponential Growth at Physiological and Atmospheric Oxygen Concentrations. PloS One, 9(11), 1-13. [More Information]
  • Martinez, V., Quek, L., Nielsen, L. (2014). Network Thermodynamic Curation of Human and Yeast Genome-Scale Metabolic Models. Biophysical Journal, 107(2), 493-503. [More Information]
  • Dal’Molin, C., Quek, L., Palfreyman, R., Nielsen, L. (2014). Plant genome-scale modeling and implementation. Methods in Molecular Biology, 1090, 317-332. [More Information]
  • Quek, L., Dietmair, S., Hanscho, M., Martinez, V., Borth, N., Nielsen, L. (2014). Reducing Recon 2 for steady-state flux analysis of HEK cell culture. Journal of Biotechnology, 184, 172-178. [More Information]
  • Martinez, V., Dietmair, S., Quek, L., Hodson, M., Gray, P., Nielsen, L. (2013). Flux balance analysis of CHO cells before and after a metabolic switch from lactate production to consumption. Biotechnology and Bioengineering, 110(2), 660-666. [More Information]
  • Dietmair, S., Hodson, M., Quek, L., Timmins, N., Gray, P., Nielsen, L. (2012). A multi-omics analysis of recombinant protein production in Hek293 cells. PloS One, 7(8), 1-15. [More Information]
  • Dietmair, S., Hodson, M., Quek, L., Timmins, N., Chrysanthopoulos, P., Jacob, S., Gray, P., Nielsen, L. (2012). Metabolite profiling of CHO cells with different growth characteristics. Biotechnology and Bioengineering, 109(6), 1404-1414. [More Information]
  • Licona-Cassani, C., Marcellin, E., Quek, L., Jacob, S., Nielsen, L. (2012). Reconstruction of the Saccharopolyspora erythraea genome-scale model and its use for enhancing erythromycin production. Antonie van Leeuwenhoek: international journal of general and molecular microbiology, 102(3), 493-502. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Nielsen, L. (2011). AlgaGEM--a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome. BMC Genomics, 12(SUPPL. 4), 1-10. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Brumbley, S., Nielsen, L. (2010). AraGEM, a genome-scale reconstruction of the primary metabolic network in Arabidopsis. Plant Physiology, 152(2), 579-589. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Brumbley, S., Nielsen, L. (2010). C4GEM, a genome-scale metabolic model to study C4 plant metabolism. Plant Physiology, 154(4), 1871-1885. [More Information]
  • Quek, L., Dietmair, S., Kromer, J., Nielsen, L. (2010). Metabolic flux analysis in mammalian cell culture. Metabolic Engineering, 12(2), 161-171. [More Information]
  • Kromer, J., Quek, L., Nielsen, L. (2009). 13C-Fluxomics: A Tool for Measuring Metabolic Phenotypes. Australian Biochemist, 40(3), 17-20.
  • Quek, L., Wittmann, C., Nielsen, L., Kromer, J. (2009). OpenFLUX: Efficient modelling software for 13C-based metabolic flux analysis. Microbial Cell Factories, 8, 1-15. [More Information]
  • Quek, L., Nielsen, L. (2008). On the reconstruction of the Mus musculus genome-scale metabolic network model. Genome Informatics, 21, 89-100.

2018

  • Small, L., Brandon, A., Quek, L., Krycer, J., James, D., Turner, N., Cooney, G. (2018). Acute activation of pyruvate dehydrogenase increases glucose oxidation in muscle without changing glucose uptake. American Journal of Physiology - Endocrinology and Metabolism, 315(2), E258-E266. [More Information]
  • Van Geldermalsen, M., Quek, L., Turner, N., Freidman, N., Pang, A., Guan, Y., Krycer, J., Ryan, R., Wang, Q., Holst, J. (2018). Benzylserine inhibits breast cancer cell growth by disrupting intracellular amino acid homeostasis and triggering amino acid response pathways. BMC Cancer, 18(1), 689 - 1-689 - 14. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Saac, P., Palfreymana, R., Nielsen, L. (2018). From reconstruction to C4 metabolic engineering: A case study for overproduction of polyhydroxybutyrate in bioenergy grasses. Plant Science, 273, 50-60. [More Information]
  • Bakshi, I., Suryana, E., Small, L., Quek, L., Brandon, A., Turner, N., Cooney, G. (2018). Fructose bisphosphatase 2 overexpression increases glucose uptake in skeletal muscle. Journal of Endocrinology, 237(2), 101-111. [More Information]
  • Buchsteiner, M., Quek, L., Gray, P., Nielsen, L. (2018). Improving culture performance and antibody production in CHO cell culture processes by reducing the Warburg effect. Biotechnology and Bioengineering, 115(9), 2315-2327. [More Information]
  • Bentley, N., Fiveash, C., Osborne, B., Quek, L., Ogura, M., Inagaki, N., Cooney, G., Polly, P., Montgomery, M., Turner, N. (2018). Protein hypoacylation induced by Sirt5 overexpression has minimal metabolic effect in mice. Biochemical and Biophysical Research Communications, 503(3), 1349-1355. [More Information]

2017

  • Krycer, J., Yugi, K., Hirayama, A., Fazakerley, D., Quek, L., Scalzo, R., Ohno, S., Hodson, M., Ikeda, S., Shoji, F., Domanova, W., Parker, B., Healy (Nelson), M., Humphrey, S., Cooney, G., James, D., et al (2017). Dynamic Metabolomics Reveals that Insulin Primes the Adipocyte for Glucose Metabolism. Cell Reports, 21(12), 3536-3547. [More Information]

2016

  • Quek, L., Liu, M., Joshi, S., Turner, N. (2016). Fast exchange fluxes around the pyruvate node: a leaky cell model to explain the gain and loss of unlabelled and labelled metabolites in a tracer experiment. Cancer and Metabolism, 4, 1-14. [More Information]
  • Couttas, T., Kain, N., Suchowerska, A., Quek, L., Turner, N., Fath, T., Garner, B., Don, A. (2016). Loss of ceramide synthase 2 activity, necessary for myelin biosynthesis, precedes tau pathology in the cortical pathogenesis of Alzheimer's disease. Neurobiology of Aging, 43(July), 89-100. [More Information]
  • Marcellin, E., Behrendorff, J., Nagaraju, S., DeTissera, S., Segovia, S., Palfreyman, R., Daniell, J., Licona-Cassani, C., Quek, L., Speight, R., et al (2016). Low carbon fuels and commodity chemicals from waste gases-systematic approach to understand energy metabolism in a model acetogen. Green Chemistry, 18(10), 3020-3028. [More Information]
  • Swainston, N., Smallbone, K., Hefzi, H., Dobson, P., Brewer, J., Hanscho, M., Zielinski, D., Ang, K., Gardiner, N., Gutierrez, J., et al (2016). Recon 2.2: from reconstruction to model of human metabolism. Metabolomics, 12(7), 1-7. [More Information]

2015

  • De Oliveira Dal'Molin, C., Quek, L., Saa, P., Nielsen, L. (2015). A multi-tissue genome-scale metabolic modeling framework for the analysis of whole plant systems. Frontiers in Plant Science, 6, 1-12. [More Information]
  • Martinez, V., Buchsteiner, M., Gray, P., Nielsen, L., Quek, L. (2015). Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism. Metabolic Engineering Communications, 2, 46-57. [More Information]

2014

  • Quek, L., Nielsen, L. (2014). A depth-first search algorithm to compute elementary flux modes by linear programming. BMC Systems Biology, 8(1), 1-10. [More Information]
  • Quek, L., Nielsen, L. (2014). Customization of 13C-MFA Strategy According to Cell Culture System. In Jens O. Kromer, Lars K. Nielsen, Lars M. Blank (Eds.), Methods in Molecular Biology: Metabolic Flux Analysis - Methods and Protocols, (pp. 81-90). New York: Humana Press. [More Information]
  • Arifin, Y., Archer, C., Lim, S., Quek, L., Sugiarto, H., Marcellin, E., Vickers, C., Kromer, J., Nielsen, L. (2014). Escherichia coli W shows fast, highly oxidative sucrose metabolism and low acetate formation. Applied Microbiology and Biotechnology, 98(21), 9033-9044. [More Information]
  • Turner, J., Quek, L., Titmarsh, D., Kromer, J., Kao, L., Nielsen, L., Wolvetang, E., Cooper-White, J. (2014). Metabolic Profiling and Flux Analysis of MEL-2 Human Embryonic Stem Cells during Exponential Growth at Physiological and Atmospheric Oxygen Concentrations. PloS One, 9(11), 1-13. [More Information]
  • Martinez, V., Quek, L., Nielsen, L. (2014). Network Thermodynamic Curation of Human and Yeast Genome-Scale Metabolic Models. Biophysical Journal, 107(2), 493-503. [More Information]
  • Dal’Molin, C., Quek, L., Palfreyman, R., Nielsen, L. (2014). Plant genome-scale modeling and implementation. Methods in Molecular Biology, 1090, 317-332. [More Information]
  • Quek, L., Dietmair, S., Hanscho, M., Martinez, V., Borth, N., Nielsen, L. (2014). Reducing Recon 2 for steady-state flux analysis of HEK cell culture. Journal of Biotechnology, 184, 172-178. [More Information]
  • Quek, L., Nielsen, L. (2014). Steady-State 13C Fluxomics Using OpenFLUX. In Jens O. Kromer, Lars K. Nielsen, Lars M. Blank (Eds.), Methods in Molecular Biology: Metabolic Flux Analysis - Methods and Protocols, (pp. 209-224). New York: Humana Press. [More Information]

2013

  • Martinez, V., Dietmair, S., Quek, L., Hodson, M., Gray, P., Nielsen, L. (2013). Flux balance analysis of CHO cells before and after a metabolic switch from lactate production to consumption. Biotechnology and Bioengineering, 110(2), 660-666. [More Information]

2012

  • Dietmair, S., Hodson, M., Quek, L., Timmins, N., Gray, P., Nielsen, L. (2012). A multi-omics analysis of recombinant protein production in Hek293 cells. PloS One, 7(8), 1-15. [More Information]
  • Dietmair, S., Hodson, M., Quek, L., Timmins, N., Chrysanthopoulos, P., Jacob, S., Gray, P., Nielsen, L. (2012). Metabolite profiling of CHO cells with different growth characteristics. Biotechnology and Bioengineering, 109(6), 1404-1414. [More Information]
  • Licona-Cassani, C., Marcellin, E., Quek, L., Jacob, S., Nielsen, L. (2012). Reconstruction of the Saccharopolyspora erythraea genome-scale model and its use for enhancing erythromycin production. Antonie van Leeuwenhoek: international journal of general and molecular microbiology, 102(3), 493-502. [More Information]

2011

  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Nielsen, L. (2011). AlgaGEM--a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome. BMC Genomics, 12(SUPPL. 4), 1-10. [More Information]

2010

  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Brumbley, S., Nielsen, L. (2010). AraGEM, a genome-scale reconstruction of the primary metabolic network in Arabidopsis. Plant Physiology, 152(2), 579-589. [More Information]
  • Gomes de Oliveira Dal'Molin, C., Quek, L., Palfreyman, R., Brumbley, S., Nielsen, L. (2010). C4GEM, a genome-scale metabolic model to study C4 plant metabolism. Plant Physiology, 154(4), 1871-1885. [More Information]
  • Quek, L., Nielsen, L. (2010). Metabolic Engineering of Mammalian Cells. In Christina D. Smolke (Eds.), The Metabolic Pathway Engineering Handbook: Fundamentals, (pp. 26-1-26-14). Florida, USA: CRC Press.
  • Quek, L., Dietmair, S., Kromer, J., Nielsen, L. (2010). Metabolic flux analysis in mammalian cell culture. Metabolic Engineering, 12(2), 161-171. [More Information]

2009

  • Kromer, J., Quek, L., Nielsen, L. (2009). 13C-Fluxomics: A Tool for Measuring Metabolic Phenotypes. Australian Biochemist, 40(3), 17-20.
  • Quek, L., Wittmann, C., Nielsen, L., Kromer, J. (2009). OpenFLUX: Efficient modelling software for 13C-based metabolic flux analysis. Microbial Cell Factories, 8, 1-15. [More Information]

2008

  • Quek, L., Nielsen, L. (2008). On the reconstruction of the Mus musculus genome-scale metabolic network model. Genome Informatics, 21, 89-100.

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