Research student profile: Sambasivam Kuppusamy Periyannan
Genetic and molecular characterization of stem rust resistance in wheat.
My project aims to understand the genetic and molecular aspects of stem rust resistance provided by Sr22, Sr13, Sr33 and Sr45 genes in wheat. Briefly, it involves development of tightly linked PCR markers, fine mapping and study on gene interaction.
Stem rust is one of the major diseases in wheat cultivation and their management through host plant resistance is found to be one of the best strategies followed for several decades. Molecular tools like markers will aid in the rapid selection of resistant lines in the breeding program. Additionally, fine mapping of genes will provide entry points towards the positional cloning of the genes which is required for the better understanding of the resistance.
To tackle this project my research involves the utilization of publicly available genomic information of wheat and their related grass members like rice, barley and Brachypodium etc. The methods like production of mapping population, rust screening, marker development, validation, mapping, and sequence analysis etc. are followed in this study.
Markers identified from this work will enable the wheat breeders to make a quick and easy selection of lines carrying the targeted gene. Information from the fine mapping will enable progress in map based cloning of the targeted gene.
I am from India and hold a B. Sc. (Horticulture) from Tamil Nadu Agricultural University, an M. Sc. (Agriculture) with Plant Pathology as my specialisation from Kerala Agricultural University, and a Postgraduate Diploma in Bioinformatics from Bharathiar University. I previously worked as Horticultural Pathologist in “Tamil Nadu Precision Farming Project” at Tamil Nadu Agricultural University for a period of three years.
I got an Indian Council of Agricultural Research - Junior Research Fellowship for my M. Sc. (Agriculture) and now I have been granted an EIPRS and IPA scholarship. I also cleared National Eligibility Test (NET) for Lectureship /Assistant Professorship conducted by Indian Council of Agricultural Research.
During the second year of my present study I attended the “11th International Wheat Genetics Symposium” held at Brisbane and presented a paper on “Identification of markers linked with stem rust resistance genes Sr33 and Sr45”.
After my M. Sc. (Agriculture), I attended the “International Conference on Science and Technology for Sustainable Development” held at Kerala and presented a paper on “Use of antibiotics for the management of Ralstonia solanacearum causing bacterial wilt disease in ginger”.
- Sambasivam PK, Bansal UK, Hayden MJ, Dvorak J, Lagudah ES, Bariana HS. 2008. Identification of markers linked with stem rust resistance genes Sr33 and Sr45. In Proceedings of the 11th International Wheat Genetics Symposium, Brisbane, Australia, 24-29th August 2008.
- Sambasivam P.K and Girija D. 2007. Molecular Characterization of Ralstonia solanacearum infecting ginger in Kerala. Journal of Ecobiology 20(3):229-237
- Sambasivam P.K and Girija D. 2006. Biochemical Characterization of Ralstonia solanacearum infecting ginger. Annals of Plant Protection Sciences 14(2): 419-423
- Sambasivam P.K and Girija D. 2005. Studies on host range and intrinsic antibiotic resistance pattern of Ralstonia solanacearum infecting ginger. Annals of Plant Protection Sciences 13(2): 431-433
- Sambasivam P.K, Girija D, Sally K Mathew. 2005. Use of antibiotics for the management of Ralstonia solanacearum causing bacterial wilt disease in ginger. In Proceedings of the International Conference on Science and Technology for Sustainable Development, Kerala, India, 10-13th August 2005.