Redox Biology Laboratory

Lab head: Dr Paul Witting
Location: Blackburn Building, Camperdown Campus

Protein modifications that potentially underlie the severity of acute myocardial infarct in hearts from diabetic rats. In addition, we have an established interest in monitoring oxidative stress in disease states including atherosclerosis, acute renal failure and cerebral ischemia injury (stroke).

Funding: ARC; National Heart Foundation; Diabetes Australia
Research approach equipment: Analytical Biochemistry: using analytical techniques such as liquid chromatography and liquid chromatography coupled with mass spectrometry, the redox biology lab assesses the mechanism of acute pathological processes. Molecular biology: we use quantitative gene analysis techniques to assess cellular and tissue response to experimental ischaemia reperfusion injury as a model for stroke and heart attack.

Post translational changes to key cardiac proteins in the hearts of diabetic rats after experimental heart attack

Primary supervisor: Paul Witting

The major causes of morbidity and mortality in diabetes are the cardiovascular complications of the disease. Of these, the development of diabetic cardiomyopathy is of particular concern, as it increases the risk and severity of acute myocardial infarction (heart attack) compared with the general population. The proposed project will provide information on the role for oxidative stress and inflammation in promoting the severity of myocardial infarct in an animal model of diabetic cardiomyopathy. We plan to use an established rat model of diabetes and monitor post translational changes to key cardiac proteins through a proteomic approach combining 2D gel and mass spectrometry peptide mass mapping. An understanding of the role for oxidative stress and inflammation following acute myocardial infarct in diabetics may allow the development of new therapeutic approaches targeted at slowing down the progression of cardiomyopathy and thereby potentially improving heart function and quality of life in diabetes sufferers. This would be viewed as a major advance.

Discipline: Pathology
Co-supervisors: Aisling McMahon