You are here: Home / research / Our Research / Research Facilities

NMR Facility

NMR Instrumentation

Varian 400 MR Spectrometer and Autosampler

The Facility has a new fully automated State of the Art Spectrometer from Varian, a 400MR System with SMS Autosampler.

The System has Pulsed Field Gradients & PFG 5mm ATB Broadband Probe 1H/19F/X, the Magnet is a Premium Shielded , long hold magent with up to 270 days Liquid Helium hold time.

The VNMRJ software runs under Red Hat. This aquisition will enable researchers to carry out the latest experiments with minimal effort and with excellent sensitivity.

Past Instruments

Varian 300 NMR Spectrometer Jeol FX90Q NMR Perkin Elmer R12 NMR
Varian Gemini 300BB NMR JEOL FX90Q FT NMR Perkin Elmer NMR
1992-2008 1980-1992 1972-1980

Techniques

Proton

Image: Proton - Click for full size

One of the most basic of all NMR experiments allows an initial view into the nature of the molecule being investigated, the spectrum can be simple to interperate or can be very complex, especially for large molecules.

The proton spectrum is the most basic and least time consuming experiment typically, taking minutes to run but some times hours or days to interpret.

Carbon

Image: Carbon Graph - Click for full size

The Carbon spectrum is another of the "simple" and basic spectrum for NMR, a wealth of information which helps unravel the structure of compounds examined by this technique, the typical carbon spectrum takes many hours to run, the natural abundance of 13C is 1.1% of its natural counterpart carbon 12.

Labelled 13 C experiments can take minutes dependant on the percentage enrichment of the isotope in the sample.

DEPT

Image: DEPT Graph - Click for full size

Distortionless enhancement by polarization transfer is a heteronuclear experiment (normally 13C -1H), the experiment determines whether a resonance signal found in the carbon spectrum is from CH3 , CH2 , CH or a quaternary carbon.

The most common results from this experiment are readily interperated as the CH3 and CH peaks are of normal phase and the CH2 peaks are of opposite phase (inverted) this is called a DEPT 135, On the GEMINI the software routine in the DEPTP Macro plots and labels the spectra in to three experiments.

APT

Image: APT - Click for full size

The Attached Proton Test is a simple experiment which helps in elucidation of structure by determining what type of Protons are attached to Carbons.

The spectrum takes as long as a carbon analysis to run but does not show and quartinary/carbonyl carbons in the resultant spectum.

COSY

Image: COSY - Click for full size

This is a 2D (2 dimensional) homonuclear experiment, normally 1H. COSY is basically like doing a whole series of the homonuclear decoupling experiments (1D) in a fully automated mode.

The results are in a 2D array which shows the spin-spin coupling between all nuclei in the sample to be presented on one single page.

HECTOR

Image: HECTOR - Click for full size

The HETCOR experiment is a hetronuclear 2D (2 dimensional) experiment typically usefully for 1H - 13C correlation. The experiment has a 1H component and a 13C component the data plotted has the 1H on one axis and the 13C on the other, the so called Carbon - Proton correlation can be assigned making structural elucidation a much easier task.