Outline

Overview

Without cells, life as we know it would not exist. These dynamic assemblies, packed with biological molecules are constantly in action. But how do cells work? Why is the food that you eat so important for cellular function? How is information transmitted from generation to generation? And, what happens as a result of disease or genetic mutation? In this unit of study, you will learn how cells work at the molecular level, with an emphasis on human biochemistry and molecular biology. We will focus initially on how genetic information is regulated in eukaryotes, including replication, transcription and translation, and molecular aspects of the cell cycle, mitosis and meiosis. Then we will explore cellular metabolism and how cells extract and store energy from fuels like fats and carbohydrates, how the use of fuels is modulated in response to exercise, starvation and disease, and how other key metabolites are processed. The advanced laboratory component will provide students with an authentic research laboratory experience while in the theory component, current research topics will be presented in a problem-based format through tutorial sessions. This material will be assessed by creative student-centered activities supported by eLearning platforms.

Unit details and rules

Academic unit	Life and Environmental Sciences Academic Operations
Credit points	6
Prerequisites ?	A mark of at least 70 from (BIOL1XX7 or MBLG1XX1) and (CHEM1XX1 or CHEM1903)
Corequisites ?	None
Prohibitions ?	BCHM2072 or BCHM2972 or MBLG2071 or MBLG2971 or BMED2405 or BCMB2001 or MEDS2003
Assumed knowledge ?	None
Available to study abroad and exchange students	Yes

Teaching staff

Coordinator	Keng Kwang Jason Low, jason.low@sydney.edu.au
Lecturer(s)	Giselle Yeo, giselle.yeo@sydney.edu.au
	Alice Huang, alice.huang@sydney.edu.au
	Keng Kwang Jason Low, jason.low@sydney.edu.au
	Matthew Clemson, matthew.clemson@sydney.edu.au

Type	Description	Weight	Due	Length
Supervised exam ?	Final exam Final exam for all lectures, practicals and tutorials.	50%	Formal exam period	2 hours
Outcomes assessed:
Online task	Electronic laboratory notebook (LabArchives) Data analysis and interpretation, laboratory calculations, assay design.	30%	Multiple weeks	Notebooks completed after each practical
Outcomes assessed:
Tutorial quiz	Molecular Biology Lecture revision quiz Short multiple-choice quiz related to lecture concepts	5%	Week 06 Due date: 31 Mar 2023 at 23:59 Closing date: 31 Mar 2023	30 minute quiz
Outcomes assessed:
Assignment	Cloning presentation Recorded presentation about new cloning techniques	10%	Week 09 Due date: 30 Apr 2023 at 23:59 Closing date: 30 Apr 2023	Five-minute video
Outcomes assessed:
Tutorial quiz	Metabolism Lecture revision quiz Short multiple-choice quiz related to lecture concepts	5%	Week 13 Due date: 26 May 2023 at 23:59 Closing date: 26 May 2023	30 minute quiz
Outcomes assessed:

Assessment summary

Detailed information of the contribution of each assessment task to the final mark can be found on Canvas.

Electronic Laboratory Notebook (ELN): One write-up for each of the four main practical classes, and a final assessment in assay design in week 12. The write-ups need to be completed 7 days after the practical session.

Revision Quizzes: Multiple choice questions based on the lecture material. The first quiz will be for molecular biology lectures and the second quiz will be for metabolism lectures.

Cloning Presentation (BCMB2901): This will involve presenting a 5-minute video about a cloning technique.

Final Exam: This will cover all material from lectures, practicals and tutorials. There are multiple choice and short answer questions.

Final exam: This assessment is compulsory and failure to attend, attempt, or submit will result in the award of an AF grade.

If a second replacement exam is required, this exam may be delivered via an alternative assessment method, such as a viva voce (oral exam). The alternative assessment will meet the same learning outcomes as the original exam. The format of the alternative assessment will be determined by the unit coordinator.

Assessment criteria

The University awards common result grades, set out in the Coursework Policy 2014 (Schedule 1).

As a general guide, a high distinction indicates work of an exceptional standard, a distinction a very high standard, a credit a good standard, and a pass an acceptable standard.

Result name	Mark range	Description
High distinction	85 - 100	Mastery of topics showing extensive integration and ability to transfer knowledge to novel contexts; treatment of tasks shows an advanced synthesis of ideas; demonstration of initiative, complex understanding and analysis; work is very well presented; all criteria addressed and learning outcomes achieved to an outstanding level
Distinction	75 - 84	Excellent achievement, consistent evidence of deep understanding and application of knowledge in biochemistry; treatment of tasks shows advanced understanding of topics; demonstration of initiative, complex understanding and analysis; work is well-presented; all criteria addressed and learning outcomes achieved to a superior level
Credit	65 - 74	Confident in explaining biochemistry processes, with evidence of solid understanding and achievement; occasional lapses indicative of unresolved issues; treatment of tasks show a good understanding of topic; work is well-presented with a minimum of errors; all criteria addressed and learning outcomes achieved to a high level
Pass	50 - 64	Satisfactory level of engagement with and understanding of topic; some inconsistencies in understanding and knowledge of biochemistry; work is adequately presented, with some errors or omissions, most criteria addressed and learning outcomes achieved to an adequate level
Fail	0 - 49	Unsatisfactory achievement and engagement with the biochemistry discipline; inadequate understanding or fundamental misunderstanding of topics; most criteria and learning outcomes not clearly or adequately addressed or achieved; lack of effort/involvement in the unit

For more information see guide to grades.

Late submission

In accordance with University policy, these penalties apply when written work is submitted after 11:59pm on the due date:

Deduction of 5% of the maximum mark for each calendar day after the due date.
After ten calendar days late, a mark of zero will be awarded.

Academic integrity

The Current Student website provides information on academic integrity and the resources available to all students. The University expects students and staff to act ethically and honestly and will treat all allegations of academic integrity breaches seriously.

We use similarity detection software to detect potential instances of plagiarism or other forms of academic integrity breach. If such matches indicate evidence of plagiarism or other forms of academic integrity breaches, your teacher is required to report your work for further investigation.

Use of generative artificial intelligence (AI) and automated writing tools

You may only use generative AI and automated writing tools in assessment tasks if you are permitted to by your unit coordinator. If you do use these tools, you must acknowledge this in your work, either in a footnote or an acknowledgement section. The assessment instructions or unit outline will give guidance of the types of tools that are permitted and how the tools should be used.

Your final submitted work must be your own, original work. You must acknowledge any use of generative AI tools that have been used in the assessment, and any material that forms part of your submission must be appropriately referenced. For guidance on how to acknowledge the use of AI, please refer to the AI in Education Canvas site.

The unapproved use of these tools or unacknowledged use will be considered a breach of the Academic Integrity Policy and penalties may apply.

Studiosity is permitted unless otherwise indicated by the unit coordinator. The use of this service must be acknowledged in your submission as detailed on the Learning Hub’s Canvas page.

Outside assessment tasks, generative AI tools may be used to support your learning. The AI in Education Canvas site contains a number of productive ways that students are using AI to improve their learning.

Learning support

Simple extensions

If you encounter a problem submitting your work on time, you may be able to apply for an extension of five calendar days through a simple extension.  The application process will be different depending on the type of assessment and extensions cannot be granted for some assessment types like exams.

Special consideration

If exceptional circumstances mean you can’t complete an assessment, you need consideration for a longer period of time, or if you have essential commitments which impact your performance in an assessment, you may be eligible for special consideration or special arrangements.

Special consideration applications will not be affected by a simple extension application.

Using AI responsibly

Co-created with students, AI in Education includes lots of helpful examples of how students use generative AI tools to support their learning. It explains how generative AI works, the different tools available and how to use them responsibly and productively.

Weekly schedule

WK	Topic	Learning activity
Week 01	1. Introduction; 2. Nucleic acid structure; 3. Prokaryotic replication	Lecture (3 hr)
Week 01	Introduction to labs	Tutorial (1 hr)
Week 02	1. Eukaryotic replication; 2. DNA synthesis in lab; 3. Eukaryotic genome	Lecture (3 hr)
Week 02	1. Genotyping PCR; 2. Cloning - Restriction digest	Practical (4 hr)
Week 03	1. Prokaryotic transcription; 2. Eukaryotic transcription; 3. Post-transcriptional processing	Lecture (3 hr)
Week 03	1. Genotyping PCR; 2. Cloning - Restriction digest	Tutorial (1 hr)
Week 04	1. Translation in prokaryotes; 2. Translation in eukaryotes; 3. Translational regulation	Lecture (3 hr)
Week 04	1. Electrophoresis of amplicons; 2. Cloning - Gibson assembly and transformation	Practical (4 hr)
Week 05	1. Molecular biology techniques part 1; 2. Molecular biology techniques part 2; 3. Molecular biology techniques part 3	Lecture (3 hr)
Week 05	1. Electrophoresis of amplicons; 2. Cloning - Gibson assembly and transformation	Tutorial (1 hr)
Week 06	1. Molecular Biology Revision 1; 2. Molecular Biology Revision 2; 3. Molecular Biology Revision 3	Lecture (3 hr)
Week 06	1. Cloning - Fluorescent protein expression	Practical (1 hr)
Week 08	1. Introduction to Metabolism; 2. Fuel Selection During Exercise; 3. Fatty Acid and Glucose Oxidation	Lecture (3 hr)
Week 08	1. Spectrophotometry; 2. Cloning - Visualise fluorescent proteins, plasmid isolation and sequencing	Practical (4 hr)
Week 09	1. Electron Transport Chain and Oxidative Phosphorylation; 2. Glycogenolysis, Lipolysis and Gluconeogenesis; 3. Gluconeogenesis, Proteolysis and Ketone Body Synthesis	Lecture (3 hr)
Week 09	1. Spectrophotometry; 2. Cloning - Visualise fluorescent proteins, plasmid isolation and sequencing	Tutorial (1 hr)
Week 10	1. Regulation of Pathways; 2. Gluconeogenesis in Detail; 3. Glycemic Responses and Glycogenesis	Lecture (3 hr)
Week 10	1. Enzyme-linked Metabolite Assay (ELMA); 2. Cloning - Sequence analysis	Practical (4 hr)
Week 11	1. Lipogenesis and Pentose Phosphate Pathway; 2. Lipoprotein and Cholesterol Metabolism; 3. Nitrogen Metabolism	Lecture (3 hr)
Week 11	1. Enzyme-linked Metabolite Assay (ELMA); 2. Cloning - Sequence analysis	Tutorial (1 hr)
Week 12	1. Integration of Metabolism; 2. Metabolism Revision 1; 3. Metabolism Revision 2	Lecture (3 hr)
Week 12	ELMA Design assay	Practical (4 hr)
Week 13	1. Metabolism Revision 3; 2. Metabolism Q&A; 3. Theory of Practical Revision	Lecture (3 hr)

Attendance and class requirements

All lectures, practicals and tutorials are delivered live. Attendance for all practicals and tutorials is compulsory. Lecture attendance is optional. All lectures will be recorded and made available.

Study commitment

Typically, there is a minimum expectation of 1.5-2 hours of student effort per week per credit point for units of study offered over a full semester. For a 6 credit point unit, this equates to roughly 120-150 hours of student effort in total.

Learning outcomes

Learning outcomes are what students know, understand and are able to do on completion of a unit of study. They are aligned with the University's graduate qualities and are assessed as part of the curriculum.

Outcomes
Graduate qualities

At the completion of this unit, you should be able to:

LO1. describe in detail the main anabolic and catabolic processes in the cell and discuss how variations in energy demand and supply affect these processes
LO2. compare and contrast the integration of anabolic and catabolic processes in the cells and predict how perturbations to these processes, including fuel selection and genetic mutation, affect the cell and whole organism
LO3. summarise the catabolic and anabolic fates of dietary nitrogen and predict how the synthesis and degradation of nitrogenous biopolymers are affected in contexts such as starvation, diabetes and cancer
LO4. describe the complexity of the eukaryotic genome and its structure in detail and identify the key constituent elements
LO5. outline the specific processes by which genetic information is transmitted from one generation to the next and analyse the flow of this information within the cell
LO6. describe and evaluate the steps involved in gene transcription and translation and evaluate the different ways by which gene expression can be regulated
LO7. evaluate the main concepts and power of modern molecular biology techniques and select the appropriate technique for specific applications in life science and medical research
LO8. explain, with examples, the difference between qualitative and quantitative measurements; obtain quantitative measurements of metabolite concentrations and enzyme activities, in an accurate and reproducible manner
LO9. adapt, develop and trouble-shoot recognised procedures for novel contexts and requirements
LO10. assess the quality of, interpret and draw conclusions from data obtained in the laboratory
LO11. summarise and identify the key points from topical biochemical data from a number of published sources; synthesise and communicate the findings.

Graduate qualities

The graduate qualities are the qualities and skills that all University of Sydney graduates must demonstrate on successful completion of an award course. As a future Sydney graduate, the set of qualities have been designed to equip you for the contemporary world.

GQ1	Depth of disciplinary expertise Deep disciplinary expertise is the ability to integrate and rigorously apply knowledge, understanding and skills of a recognised discipline defined by scholarly activity, as well as familiarity with evolving practice of the discipline.
GQ2	Critical thinking and problem solving Critical thinking and problem solving are the questioning of ideas, evidence and assumptions in order to propose and evaluate hypotheses or alternative arguments before formulating a conclusion or a solution to an identified problem.
GQ3	Oral and written communication Effective communication, in both oral and written form, is the clear exchange of meaning in a manner that is appropriate to audience and context.
GQ4	Information and digital literacy Information and digital literacy is the ability to locate, interpret, evaluate, manage, adapt, integrate, create and convey information using appropriate resources, tools and strategies.
GQ5	Inventiveness Generating novel ideas and solutions.
GQ6	Cultural competence Cultural Competence is the ability to actively, ethically, respectfully, and successfully engage across and between cultures. In the Australian context, this includes and celebrates Aboriginal and Torres Strait Islander cultures, knowledge systems, and a mature understanding of contemporary issues.
GQ7	Interdisciplinary effectiveness Interdisciplinary effectiveness is the integration and synthesis of multiple viewpoints and practices, working effectively across disciplinary boundaries.
GQ8	Integrated professional, ethical, and personal identity An integrated professional, ethical and personal identity is understanding the interaction between one’s personal and professional selves in an ethical context.
GQ9	Influence Engaging others in a process, idea or vision.

Outcome map

Learning outcomes	Graduate qualities
Learning outcomes

Responding to student feedback

This section outlines changes made to this unit following staff and student reviews.

In response to student feedback: 1. the number of assessments has been reduced; 2. the tutorials support the practical assessments; 3. additional revision sessions will be delivered in weeks 6 and 13

Additional information

Science student portal

Work, health and safety

We are governed by the Work Health and Safety Act 2011, Work Health and Safety Regulation 2011 and Codes of Practice. Penalties for non-compliance have increased. Everyone has a responsibility for health and safety at work. The University’s Work Health and Safety policy explains the responsibilities and expectations of workers and others, and the procedures for managing WHS risks associated with University activities.

General Laboratory Safety Rules

No eating or drinking is allowed in any laboratory under any circumstances
A laboratory coat and closed-toe shoes are mandatory
Follow safety instructions in your manual and posted in laboratories
In case of fire, follow instructions posted outside the laboratory door
First aid kits, eye wash and fire extinguishers are located in or immediately outside each laboratory
As a precautionary measure, it is recommended that you have a current tetanus immunisation. This can be obtained from University Health Service: unihealth.usyd.edu.au

Disclaimer

The University reserves the right to amend units of study or no longer offer certain units, including where there are low enrolment numbers.

To help you understand common terms that we use at the University, we offer an online glossary.

Current students

Overview

Overview

Unit details and rules

Teaching staff

Assessment

Assessment

Assessment summary

Assessment criteria

Late submission

Academic integrity

Learning support

Learning support

Simple extensions

Special consideration

Using AI responsibly

Weekly schedule

Weekly schedule

Attendance and class requirements

Study commitment

Learning outcomes

Learning outcomes

Graduate qualities

Outcome map

Responding to student feedback

Responding to student feedback

Additional information

Additional information

Work, health and safety

Disclaimer

On this page

Useful links

Media

Student links

About us

Connect

Current students

BCMB2901: Biochemistry and Molecular Biology (Advanced)

Semester 1, 2023 [Normal day] - Camperdown/Darlington, Sydney

Overview

Overview

Unit details and rules

Teaching staff

Assessment

Assessment

Assessment summary

Assessment criteria

Late submission

Academic integrity

Learning support

Learning support

Simple extensions

Special consideration

Using AI responsibly

Weekly schedule

Weekly schedule

Attendance and class requirements

Study commitment

Learning outcomes

Learning outcomes

Graduate qualities

Outcome map

Responding to student feedback

Responding to student feedback

Additional information

Additional information

Work, health and safety

Disclaimer

On this page

Useful links

Media

Student links

About us

Connect