News/Workshops

NEW TECHNOLOGIES

ZEISS LSM800 Confocal Microscope with Airyscan

Zeiss LSM

This next generation confocal microscope – the ZEISS LSM 800 plus Airyscan features two highly sensitive GaAsP detectors that provide the highest signal-to-noise ratio and together with the unique that provide the highest signal-to-noise ratio and together with the unique.Airyscan technology provide a pronounced improvement in x, y and z resolution. As a result of these features, the imaging speed is markedly increased so that more time is available for image acquisition at the microscope and for more users. This system is equipped with the following diode lasers: 405, 488, 561 and 640 nm for routine multi-channel imaging. It also includes the ‘Shuttle and Find’ module for correlative microscopy.

This system is currently located in Rm S448, Anderson Stuart Building F13 at the Bosch Institute, Advanced Microscopy Facility, The University of Sydney.

Funding for this brand new microscope was kindly provided by a 2016 Ian Potter Foundation Grant and a 2016 Cancer Institute NSW Research Equipment Grant as well as generous support funding from the DVCR, Faculty of Medicine, School of Medical Sciences, Bosch Institute, Physiology, Pathology, Anatomy & Histology, Pharmacology and the Advanced Microscopy Facility.


Life Canvas Technologies Automated Tissue Clearing System

Life Canvas Technologies

This Automated Tissue Clearing System is based upon the CLARITY protocol that enables transformation of tissue into a hydrogel-hybridized format that:

  • is fully assembled and intact
  • is optically transparent
  • is permeable to macromolecules eg. antibodies for immunofluorescence labelling studies
  • avoids tissue sectioning

The technology is an all-in-one seamless operation for hydrogel formation and active tissue clearing that reduces the cost of solutions used, speeds up the clearing process and helps avoid tissue deformation as well as preserving endogenous fluorescent signals. Since this system speeds up the sample preparation process by considerably shortening the timeframe for clearing tissues (~1-2 days for a mouse brain), it also facilitates higher throughput of samples.

Once transparent and labelled, the cleared samples (eg. whole mouse or rat brain, mouse embryo, heart tissue and more) can be imaged with a light-sheet, confocal or multi-photon microscope. At the Bosch AMF, we have the following microscopes that can be set up to image a range of cleared samples:

  • LaVision BioTec Ultramicroscope II
  • LaVision BioTec TriM Scope II Multiphoton microscope
  • ZEISS LSM 510 Meta confocal microscope
  • NEW: ZEISS LSM 800 confocal microscope plus Airyscan

For more information about this Automated Tissue Clearing technology, please go to the following webpage.

This Automated Tissue Clearing technology will be available from late July 2017 and will be located in Rm S448, Anderson Stuart Building F13 at the Bosch Institute, Advanced Microscopy Facility, The University of Sydney.

Funding for this technology was kindly provided by a University of Sydney and NHMRC Small Equipment Grant 2016.

For more information about how to access these new technologies, please email Dr Louise Cole, AMF Facility Manager directly:.


Summer/Autumn 2017 Workshops and Training Sessions

workshop image

We are pleased to announce the 2017 Summer/Autumn workshop series at the Bosch AMF.

If you are interested in learning how to use light and fluorescence microscopes, the Bosch Institute Advanced Microscopy Facility (AMF) can offer expert training and support on a range of light and fluorescence microscopes and other specialized imaging methods, including image analysis and processing methods. Download the flyer for full details of all microscope training sessions.

Summary of Summer/Autumn Workshops 2017

  • ZEISS transmitted light, fluorescence and deconvolution Microscope
  • ZEISS AxioScan Slide-Scanning Microscope, Bright-field and Fluorescence
  • Olympus MBF/Stereo Investigator TL Microscope
  • Leica SPEII confocal Microscope
  • ZEISS LSM 510 Meta confocal Microscope
  • PALM Microbeam DuoFlex Combi Laser Microdissection Microscope
  • Light-sheet/Ultramicroscope microscope
  • LaVision TriM Scope II multiphoton microscope

Workshops are FREE to current registered users of the Bosch Institute Advanced Microscopy Facility.

For workshop registration or any further information, please email .


Summer/Autumn 2016 Workshops and Training Sessions

AxioScan.Z1 image

We are pleased to announce the 2016 Summer/Autumn workshop series at the Bosch AMF.

If you are interested in learning how to use light and fluorescence microscopes, the Bosch Institute Advanced Microscopy Facility (AMF) can offer expert training and support on a range of light and fluorescence microscopes and other specialized imaging methods, including image analysis and processing methods. Download the flyer for full details of all microscope training sessions.

Summary of Summer/Autumn Workshops 2016

  • ZEISS AxioScan Slide-Scanning Microscope
  • ZEISS transmitted light, fluorescence, ApoTome.2 and deconvolution Microscope
  • NuanceĀ® multi-spectral imaging system
  • Olympus MBF/Stereo Investigator TL Microscope
  • Leica SPEII confocal Microscope
  • ZEISS LSM 510 Meta confocal Microscope
  • PALM Microbeam DuoFlex Combi Laser Microdissection Microscope
  • Light-sheet/Ultramicroscope microscope

Workshops are FREE to current registered users of the Bosch Institute Advanced Microscopy Facility.

For workshop registration or any further information, please email .


Light-Sheet Ultramicroscope II Meeting

Light-Sheet Meeting Thursday 5 November 2015

The Ultramicroscope II/light-sheet (LISH) meeting will take place on Thursday November 5th (and Friday Nov 6th for hands-on sessions only) 2015 at the Bosch Institute/School of Medical Sciences, Advanced Microscopy Facility, Anderson Stuart Building F13 at The University of Sydney.

The aim of this meeting is to gather together researchers interested in (i) the use of clearing protocols for confocal and light-sheet (LISH) imaging, (ii) the LaVision BioTec Ultramicroscope II and (iii) as a result, large data handling. Hands-on sessions (up to 2 h) on the Ultramicroscope can be booked for Friday Nov 6th.

Registration for this meeting is FREE but places are limited. To register please RSVP to Louise Cole () directly. Lunch, morning and afternoon tea is kindly provided by the meeting sponsors.

Download the event flyer here (PDF 167 KB).


NEW! AMF user group meeting

You are invited to join the first AMF user group meeting! The aim of this meeting is to allow AMF users to talk about their microscopy-related research, to share preparation methods and labelling tips and tricks, and to learn from others.

We are thrilled to have so many great speakers to start this event off. If successful, there will be a few of these meetings organised per year. This meeting is intended for both those new to microscopy as well as experienced microscopists. Do invite your colleagues that you think will be interested in this event!

Speakers:

AMF user group meeting
  • Dr Prachi Mehta, Translational Neuroscience Facility, UNSW - Gene expression profiling of motor neurons below a spinal cord injury using Laser Capture Microdissection system
  • James Kang, Laboratory of Neural Structure & Function, Anatomy & Histology - Investigating TPH neurocircuitry in the medial prefrontal cortex of the rat after nerve injury, using the Olympus MBF/Stereology microscope
  • Dr Alison Hey-Cunningham, Department of Obstetrics, Gynaecology and Neonatology - Recent experiences with MetaMorph: Brightfield image analysis for endometriosis projects
  • Jessica Dudley, Cell & Reproductive Biology Laboratory, Anatomy & Histology - Using Immunofluorescence microscopy to identify specific molecular changes to the uterine epithelium during pregnancy
  • Claude Dennis, Neuropathology, Pathology - A quantitative approach to characterising neurogenesis in the human brain

Date: Tuesday 22 September, 2015
Time: 2:00 PM - 3:30 PM.
Venue: E401, Anderson Stuart Building (F13)

Refreshments will be provided.

Click here to download the flyer (PDF 570 KB).

If you would like to attend, please email .


Improvements in immunofluorescence labelling, imaging and analysis methods

Nuance Multi-Spectral Imaging System

Are you struggling with background labelling or autofluorescence of your samples, cross-reactvity with your immunolabelling methods, low fluorescence labelling of your samples, live cell imaging and/or simply understanding fluorescence microscopy? Interested in using the Nuance multispectral imaging system at the Bosch Institute, Advanced Microscopy Facility? Come to this workshop to find out more!

Date: Tuesday 11 August 2015
Time: 9:00 AM to 12:00 PM
Venue: Room E401, Anderson Stuart Building (F13)

Morning tea will be provided.

To view the full program, please click to download the flyer (PDF 409 KB).


Winter/Spring 2015 Workshops and Training Sessions

We are pleased to announce the 2015 Winter/Spring workshop series at the Bosch AMF. Please download the flyer for full details of all Q&A, demonstration and microscope training sessions.

Highlights include:

Winter/Spring Workshops
  • ZEISS LSM 800 confocal microscope with Airyscan – First time in Australia! Demonstrations for a limited time only. Monday 13 July to Wednesday 15 July. Rm S448 Anderson Stuart Building F13.
  • NIKON A1R+ confocal microscope on demo! Demonstrations and hands-on sessions for a limited time only. Monday 20 July to Friday 14 August inclusive. Rm S448 Anderson Stuart Building F13.
  • Training sessions on Bosch AMF microscopes – ZEISS decon microscope, Leica SPEII confocal, ZEISS AxioScan slide scanner, Nuance Multi-spectral imaging system, Light-sheet Ultramicroscope and ZEISS LSM 510 Meta confocal.

For more information or to register for a Q&A session, demonstration or microscope training, please email .


Bosch Institute User Group Meeting

You are invited to attend the upcoming Bosch Institute User Group Meeting. With all Bosch Core Facilities represented, this meeting will showcase some of the interesting research being carried out by current users of the Bosch Institute Core Facilities.

Speakers:

Bosch Institute User Group Meeting
  • Sadaf Kalam (Anatomy & Histology)
  • Aviva Levina (Chemistry)
  • Nicholas Skladnev (Physiology)
  • Rachel Shparberg (Physiology)
  • Felix Marsh-Wakefield (Infectious Diseases & Immunology)
  • Denise Tam (Pharmacology)

Date: Thursday 18 June 2015
Time: 2:00 PM to 4:00 PM
Venue: N248, Anderson Stuart Building (F13)

Click here to RSVP.

Please click for the full program (PDF 129KB).

Special Announcement: At approx. 3:35 PM the winners of the Bosch Institute Advanced Microscopy Facility Micrograph Of The Year (MOTY) 2015 competition will be announced.

Refreshments will be served from 3:45 PM. Please RSVP for catering purposes.


Special Seminar: Professor Hans Ulrich Dodt

Prof. Hans Ulrich Dodt

Speaker: Professor Hans Ulrich Dodt
Centre for Brain Research, Medical University of Vienna and Vienna University of Technology
Title: Imaging of cleared biological samples with the Ultramicroscope
Date: Friday March 20, 2015
Time: 3:00 PM - 4:00 PM.
Seminar will be followed by wine and cheese.
Venue: N248, Anderson Stuart Building (F13), The University of Sydney
RSVP: To for catering purposes.

Please click to read more.