NanoDrop ND-1000 Spectrophotometer
- Spectrophotometer for full-spectrum UV-Vis absorbance analysis (220-750nm)
- Quantifies nucleic acids, proteins and fluorescent dyes
- Large dynamic range: 2-3700ng/µl of dsDNA
- Requires only 1µl samples with no cuvettes or capillaries needed
- Ten second measurements
- Data can be exported to spreadsheet
Manual: Nanodrop user manual
Use of the system in the Molecular Biology Facility
- There are Nanodrop spectrophotometers in both Anderson Stuart and Blackburn facilities Anderson Stuart online booking and Blackburn online booking calendar. Please make sure you book the right one. Plan ahead, book time slots and stick to them. You should not need to book more than half hour time slots for the NanoDrop.
- The NanoDrop is set up so that you need to have a log on to use it. The Molecular Biology Officer can give you a log on once she is satisfied you have had training and know how to use the machine. This means that the use of the machine can be monitored and, if something goes wrong with the machine, the last user can be identified. Hence, do not give your log on information to someone else.
- Never lift the sample arm by the black fibre optic cable! Only touch the sample arm itself.
- Wiping the sample from the upper and lower pedestals (with a soft laboratory wipe) following each measurement is sufficient to prevent sample carryover and avoid residue buildup. All the same, please clean the surfaces with de-ionised water after your last measurement.
- Data is automatically stored in your user folder. The files can be opened by the integrated Data Viewer software program or spreadsheet programs such as MS Excel. It is preferable that you transfer the data to your own server for archiving and printing. Alternatively, take the data away on a USB memory stick, or just record the data in you lab book. Data left on the computer may be cleaned out/deleted at any time.
- If you are the last user for the day, please shut down the computer after use.
Tips and additional information
- Sample size is not critical to measurements, however, it is essential that the liquid column is formed between the upper and lower measurement pedestals. If you use a precision pipettor and tips, a 1µl sample should provide reproducible readings with aqueous solutions of nucleic acids. If the sample column is not forming properly between the upper and lower measurement pedestals then you may need to add more sample (e.g. 2µl instead of 1µl). Alternatively, "buff" the surfaces by rubbing each with a dry laboratory wipe 15-20 times in order to "re-condition" the surfaces.
- If you are not getting acceptable duplicate readings, check your sample homogeneity. Often the problem is incomplete resuspension of your sample.
- If your sample is precious, it is possible to recover it from the upper and lower measurement pedestals by extraction with a pipette.