Sydney Mass Spectrometry

Sydney Mass Spectrometry provides access to cutting-edge instrumentation and expert assistance for proteomics, glycomics, metabolomics, lipidomics, and mass spectrometry imaging.

Our facilities feature a diverse portfolio of 20 mass spectrometers, with extensive data processing and analysis packages, and are located on two sites: the Charles Perkins Centre at the University of Sydney and the Kolling Institute at the Royal North Shore Hospital. 

Our specialist technical staff have over 50 years’ combined experience and are on hand to provide high-level training, project development, and contract research services.

• Academic Director – Professor Stuart Cordwell
• Associate Director – Dr Ben Crossett
• Senior Administration Officer – Jacquie Kufner

We welcome enquiries regarding our range of research services available on a contractual basis. Examples of our services include:

• Whole mass analysis – checks the quality and purity of synthetic peptides or purified proteins. It can be successfully applied to proteins up 175 kDa in size
• Peptide mass fingerprinting (PMF) – commonly used to identify proteins from 1D or 2D gels. It can be used to determine approximate sites of cleavage in the event of partial proteolysis of purified proteins, or the incorporation of labels such as deuterium, N15 or selenomethionine
• One dimensional liquid chromatography coupled to tandem mass spectrometry (1D LC-MS) can be used to identify proteins. Unlike PMF, 1D LC-MS can identify 10-100’s of proteins in semi-complex mixtures. Typical applications include identifying binding partners in pull-down assays
• Quantitative shotgun proteomics – for a comprehensive analysis of complex protein mixtures which are first digested with a protease and then the peptides are separated by multidimensional liquid chromatography. It typically identifies and quantifies thousands of proteins.