Associate Professor Renae Ryan

Associate Professor
Pharmacology, School of Medical Sciences

Telephone +61 2 9351 2669
Fax +61 2 9351 3868

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Research interests

Dr. Ryan's research focuses on understanding the way in which proteins move molecules across the cell membrane. In particular, she studies transporters for the neurotransmitters glutamate and glycine that play an important role in regulating normal neurotransmission in the brain. The dysfunction of these transporters has been implicated in disease states such as Alzheimer's disease, schizophrenia and ischemia following a stroke. Dr. Ryan uses a variety of biochemical and molecular techniques to try and understand the mechanism of transport and also how drugs interact with these proteins.

PhD and master's project opportunities

Selected grants

2015

  • Mechanism of anoxic iron acquisition in pathogenic bacteria; Jormakka M, Maher M, Ryan R; National Health and Medical Research Council (NHMRC)/Project Grants.
  • Novel Drugs for the Treatment of Chronic Pain; Vandenberg R, O'Mara M, Imlach W, Rawling T, Carland J, Ryan R; National Health and Medical Research Council (NHMRC)/Project Grants.

2014

  • CLARIOstar Multifunctional Microplate Reader for Shared Used at the Open Access, Multi-User Molecular Biology Core Facility; Huang M, Scolyer R, Fraser S, Sahni S, Speranza T, Owens T, Barrs V, Ju Y, Kovacevic Z, Lu Z, Camp A, Slobedman B, Morris B, Oliver B, Dos Remedios C, Kalinowski D, Fu D, Lai D, Lovicu F, Allbutt H, Triccas J, Burgess J, Kril J, Dixon K, Rendina L, Byrne M, King N, Groundwater P, Williamson P, Ho J, Dong Q, Bao B, Assinder S, McLennan S, Richardson D, Ammit A, Sharland A, Hardikar A, Hong A, Hambly B, Lee C, Murphy C, Goldsbury C, Johnstone D, Lane D, Hibbs D, Joshua D, Huq F, Halliday G, Sutherland G, Zreiqat H, Lok H, Lyons G, Jansson P, Black J, Zhu L, Sunde M, Day M, Naylor M, Buckland M, Murray M, Shafie N, Lay P, Poronnik P, Codd R, Mason R, Ryan R, Christopherson R, de Graaf S; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2013

  • Multiangle laser light scattering instrument for protein characterisation; Mackay J, Ataide S, Jormakka M, Ryan R, Matthews J, Shepherd N, Sunde M, Collyer C, Payne R; National Health and Medical Research Council (NHMRC)/Equipment Grants.
  • The structural basis for glutamate transporter function; Ryan R, Vandenberg R; National Health and Medical Research Council (NHMRC)/Project Grants.

2012

  • Lipid Modulation of Glycine Transporters; Vandenberg R, Ryan R; National Health and Medical Research Council (NHMRC)/Project Grants.

2011

  • PyroMark Q96 ID system; Richardson D, Stone J, Black J, King N, Mason R, Hunt N, Kril J, Murray M, Scolyer R, Halliday G, Vandenberg R, McMinn P, Chadban S, Byrne M, Ryan R, Dong Q, Lee C, Bishop A, Haass N, Fraser S, Hambly B, Sharland A, Kalinowski D, Assinder S, Yu Y, Bao B, Ju Y, Lovejoy D, Buckland M, Lane D, Dos Remedios C, Kovacevic Z, Sutherland G, Zhang D, Morris B, Suryo Rahmanto A, Murphy C; National Health and Medical Research Council (NHMRC)/Equipment Grants.

2010

  • Mechanism of glutamate transport from experimental and simulation studies; Kuyucak S, Ryan R, Vandenberg R; Australian Research Council (ARC)/Discovery Projects (DP).

2009

  • The Molecular mechanism of ion-coupled transport in the brain; Ryan R; National Health and Medical Research Council (NHMRC)/Career Development Awards.
  • Drug binding Sites on Glycine Transporters; Vandenberg R, Ryan R; National Health and Medical Research Council (NHMRC)/Project Grants.
  • The Molecular mechanism of ion-coupled transport in the brain; Ryan R; DVC Research/Research Fellow Establishment Grants.

2007

  • Molecular Dynamics of Glutamate Transport; Vandenberg R, Ryan R; National Health and Medical Research Council (NHMRC)/Project Grants.

2005

  • Investigations Into The Crystal Structure And Function Of A Bacterial Glutamate Transporter; Ryan R; National Health and Medical Research Council (NHMRC)/Early Career Fellowships (ECF).

Selected publications

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Book Chapters

  • Carland, J., Edington, A., Scopelliti, A., Ryan, R., Vandenberg, R. (2013). Directed Mutagenesis in Structure Activity Studies of Neurotransmitter Transporters. In David Figurski (Eds.), Genetic Manipulation of DNA and Protein - Examples from Current Research, (pp. 167-184). Rijeka: InTech Publishers.
  • Ryan, R., Boudker, O. (2013). Glutamate Transporter Family. In Gordon C. K. Roberts (Eds.), Encyclopedia of Biophysics, (pp. 893-900). Heidelberg: Springer.
  • McKinzie, A., Ryan, R., Vandenberg, R. (2010). Site-Directed Mutagenesis in the Study of Membrane Transporters. In Qing Yan (Eds.), Membrane Transporters in Drug Discovery and Development: Methods and Protocols - Methods in Molecular Biology Volume 637, (pp. 277-293). New York: Humana Press.
  • Ryan, R., Mitrovic, A., Vandenberg, R. (2003). Site-directed mutagenesis in the study of membrane transportes. In Qing Yan (Eds.), Membrane Transporters: Methods and Protocols (Methods in Molecular Biology Vol. 227), (pp. 97-108). New Jersey, USA: Humana Press.

Journals

  • Carland, J., Handford, C., Ryan, R., Vandenberg, R. (2014). Lipid Inhibitors of High Affinity Glycine Transporters: Identification of a novel class of analgesics. Neurochemistry International: the journal for the publication of cellular and molecular aspects of neurochemistry, 73, 211-216. [More Information]
  • Scopelliti, A., Heinzelmann, G., Kuyucak, S., Ryan, R., Vandenberg, R. (2014). Na+ interactions with the neutral amino acid transporter. The Journal of Biological Chemistry, 289(25), 17468-17479. [More Information]
  • Wang, K., Beaumont, K., Otte, N., Font Sadurni, J., Bailey, C., van Geldermalsen, M., Sharp, D., Tiffen, J., Ryan, R., Jormakka, M., Haass, N., Rasko, J., Holst, J. (2014). Targeting glutamine transport to suppress melanoma cell growth. International Journal of Cancer, 135(5), 1060-1071. [More Information]
  • Cater, R., Vandenberg, R., Ryan, R. (2014). The Domain Interface of the Human Glutamate Transporter EAAT1 Mediates Chloride Permeation. Biophysical Journal, 107(3), 621-629. [More Information]
  • Vandenberg, R., Ryan, R. (2013). Mechanisms of glutamate transport. Physiological Reviews, 93(4), 1621-1657. [More Information]
  • Scopelliti, A., Ryan, R., Vandenberg, R. (2013). Molecular Determinants for Functional Differences between Alanine-Serine-Cysteine Transporter 1 and Other Glutamate Transporter Family Members. The Journal of Biological Chemistry, 288(12), 8250-8257. [More Information]
  • Carland, J., Mansfield, R., Ryan, R., Vandenberg, R. (2013). Oleoyl-L-carnitine inhibits glycine transport by GlyT2. British Journal of Pharmacology, 168(4), 891-902. [More Information]
  • Bastug, T., Heinzelmann, G., Kuyucak, S., Salim, M., Vandenberg, R., Ryan, R. (2012). Position of the Third Na+ Site in the Aspartate Transporter GltPh and the Human Glutamate Transporter, EAAT1. PLoS One, 7(3), 1-10. [More Information]
  • Bailey, C., Ryan, R., Theong, A., Ng, C., King, K., Vanslambrouck, J., Auray-Blais, C., Vandenberg, R., Bröer, S., Rasko, J. (2011). Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria. Journal of Clinical Investigation, 121(1), 446-453. [More Information]
  • Vandenberg, R., Handford, C., Campbell, E., Ryan, R., Yool, A. (2011). Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1. The Biochemical Journal, 439(2), 333-340. [More Information]
  • Ryan, R., Kortt, N., Sirivanta, T., Vandenberg, R. (2010). The position of an arginine residue influences substrate affinity and K+ coupling in the human glutamate transporter, EAAT1. Journal of Neurochemistry, 114(2), 565-575. [More Information]
  • Edington, A., McKinzie, A., Reynolds, A., Kassiou, M., Ryan, R., Vandenberg, R. (2009). Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonylglycine inhibition of glycine transport. Journal of Biological Chemistry, 284(52), 36424-36430. [More Information]
  • Ryan, R., Compton, E., Mindell, J. (2009). Functional characterization of a Na+-dependent aspartate transporter from Pyrococcus horikoshii. Journal of Biological Chemistry, 284(26), 17540-17548. [More Information]
  • Huang, S., Ryan, R., Vandenberg, R. (2009). The Role of Cation Binding in Determining Substrate Selectivity of Glutamate Transporters. Journal of Biological Chemistry, 284(7), 4510-4515. [More Information]
  • Vandenberg, R., Huang, S., Ryan, R. (2008). Slips, leaks and channels in glutamate transporters. Channels, 2(1), 51-58. [More Information]
  • Boudker, O., Ryan, R., Yernool, D., Shimamoto, K., Gouaux, E. (2007). Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter. Nature, 445(7126), 387-393. [More Information]
  • Ryan, R., Mindell, J. (2007). The uncoupled chloride conductance of a bacterial glutamate transporter homolog. Nature Structural and Molecular Biology, 14(5), 365-371. [More Information]
  • Ryan, R., Vandenberg, R. (2005). A channel in a transporter. Clinical and Experimental Pharmacology and Physiology, 32(1-2), 1-6. [More Information]
  • Vandenberg, R., Ryan, R. (2005). How and Why Are Channels in Transporters? Signal Transduction Knowledge Environment, 19(280), pe17. [More Information]
  • Vandenberg, R., Ju, P., Aubrey, K., Ryan, R., Mitrovic, A. (2004). Allosteric Modulation Of Neurotransmitter Transporters At Excitatory Synapses. European Journal of Pharmaceutical Sciences, 23(1), 1-11.
  • Jennings, E., Ryan, R., Christie, M. (2004). Effects Of Sumatriptan On Rat Medullary Dorsal Horn Neurons. Pain, 111(1-2), 30-37.
  • Ryan, R., Mitrovic, A., Vandenberg, R. (2004). The Chloride Permeation Pathway Of A Glutamate Transporter And Its Proximity To The Glutamate Translocation Pathway. Journal of Biological Chemistry, 279(20), 20742-20751.
  • Ryan, R., Vandenberg, R. (2002). Distinct conformational states mediate the transport and anion channel properties of the glutamate transporter EAAT-1. Journal of Biological Chemistry, 277(16), 13494-13500.
  • Borgland, S., Connor, M., Ryan, R., Ball, H., Christie, M. (2002). Prostaglandin E2 inhibits calcium current in two sub-populations of acutely isolated mouse trigeminal sensory neurons. The Journal of Physiology, 539(2), 433-444.

2014

  • Carland, J., Handford, C., Ryan, R., Vandenberg, R. (2014). Lipid Inhibitors of High Affinity Glycine Transporters: Identification of a novel class of analgesics. Neurochemistry International: the journal for the publication of cellular and molecular aspects of neurochemistry, 73, 211-216. [More Information]
  • Scopelliti, A., Heinzelmann, G., Kuyucak, S., Ryan, R., Vandenberg, R. (2014). Na+ interactions with the neutral amino acid transporter. The Journal of Biological Chemistry, 289(25), 17468-17479. [More Information]
  • Wang, K., Beaumont, K., Otte, N., Font Sadurni, J., Bailey, C., van Geldermalsen, M., Sharp, D., Tiffen, J., Ryan, R., Jormakka, M., Haass, N., Rasko, J., Holst, J. (2014). Targeting glutamine transport to suppress melanoma cell growth. International Journal of Cancer, 135(5), 1060-1071. [More Information]
  • Cater, R., Vandenberg, R., Ryan, R. (2014). The Domain Interface of the Human Glutamate Transporter EAAT1 Mediates Chloride Permeation. Biophysical Journal, 107(3), 621-629. [More Information]

2013

  • Carland, J., Edington, A., Scopelliti, A., Ryan, R., Vandenberg, R. (2013). Directed Mutagenesis in Structure Activity Studies of Neurotransmitter Transporters. In David Figurski (Eds.), Genetic Manipulation of DNA and Protein - Examples from Current Research, (pp. 167-184). Rijeka: InTech Publishers.
  • Ryan, R., Boudker, O. (2013). Glutamate Transporter Family. In Gordon C. K. Roberts (Eds.), Encyclopedia of Biophysics, (pp. 893-900). Heidelberg: Springer.
  • Vandenberg, R., Ryan, R. (2013). Mechanisms of glutamate transport. Physiological Reviews, 93(4), 1621-1657. [More Information]
  • Scopelliti, A., Ryan, R., Vandenberg, R. (2013). Molecular Determinants for Functional Differences between Alanine-Serine-Cysteine Transporter 1 and Other Glutamate Transporter Family Members. The Journal of Biological Chemistry, 288(12), 8250-8257. [More Information]
  • Carland, J., Mansfield, R., Ryan, R., Vandenberg, R. (2013). Oleoyl-L-carnitine inhibits glycine transport by GlyT2. British Journal of Pharmacology, 168(4), 891-902. [More Information]

2012

  • Bastug, T., Heinzelmann, G., Kuyucak, S., Salim, M., Vandenberg, R., Ryan, R. (2012). Position of the Third Na+ Site in the Aspartate Transporter GltPh and the Human Glutamate Transporter, EAAT1. PLoS One, 7(3), 1-10. [More Information]

2011

  • Bailey, C., Ryan, R., Theong, A., Ng, C., King, K., Vanslambrouck, J., Auray-Blais, C., Vandenberg, R., Bröer, S., Rasko, J. (2011). Loss-of-function mutations in the glutamate transporter SLC1A1 cause human dicarboxylic aminoaciduria. Journal of Clinical Investigation, 121(1), 446-453. [More Information]
  • Vandenberg, R., Handford, C., Campbell, E., Ryan, R., Yool, A. (2011). Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1. The Biochemical Journal, 439(2), 333-340. [More Information]

2010

  • McKinzie, A., Ryan, R., Vandenberg, R. (2010). Site-Directed Mutagenesis in the Study of Membrane Transporters. In Qing Yan (Eds.), Membrane Transporters in Drug Discovery and Development: Methods and Protocols - Methods in Molecular Biology Volume 637, (pp. 277-293). New York: Humana Press.
  • Ryan, R., Kortt, N., Sirivanta, T., Vandenberg, R. (2010). The position of an arginine residue influences substrate affinity and K+ coupling in the human glutamate transporter, EAAT1. Journal of Neurochemistry, 114(2), 565-575. [More Information]

2009

  • Edington, A., McKinzie, A., Reynolds, A., Kassiou, M., Ryan, R., Vandenberg, R. (2009). Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonylglycine inhibition of glycine transport. Journal of Biological Chemistry, 284(52), 36424-36430. [More Information]
  • Ryan, R., Compton, E., Mindell, J. (2009). Functional characterization of a Na+-dependent aspartate transporter from Pyrococcus horikoshii. Journal of Biological Chemistry, 284(26), 17540-17548. [More Information]
  • Huang, S., Ryan, R., Vandenberg, R. (2009). The Role of Cation Binding in Determining Substrate Selectivity of Glutamate Transporters. Journal of Biological Chemistry, 284(7), 4510-4515. [More Information]

2008

  • Vandenberg, R., Huang, S., Ryan, R. (2008). Slips, leaks and channels in glutamate transporters. Channels, 2(1), 51-58. [More Information]

2007

  • Boudker, O., Ryan, R., Yernool, D., Shimamoto, K., Gouaux, E. (2007). Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter. Nature, 445(7126), 387-393. [More Information]
  • Ryan, R., Mindell, J. (2007). The uncoupled chloride conductance of a bacterial glutamate transporter homolog. Nature Structural and Molecular Biology, 14(5), 365-371. [More Information]

2005

  • Ryan, R., Vandenberg, R. (2005). A channel in a transporter. Clinical and Experimental Pharmacology and Physiology, 32(1-2), 1-6. [More Information]
  • Vandenberg, R., Ryan, R. (2005). How and Why Are Channels in Transporters? Signal Transduction Knowledge Environment, 19(280), pe17. [More Information]

2004

  • Vandenberg, R., Ju, P., Aubrey, K., Ryan, R., Mitrovic, A. (2004). Allosteric Modulation Of Neurotransmitter Transporters At Excitatory Synapses. European Journal of Pharmaceutical Sciences, 23(1), 1-11.
  • Jennings, E., Ryan, R., Christie, M. (2004). Effects Of Sumatriptan On Rat Medullary Dorsal Horn Neurons. Pain, 111(1-2), 30-37.
  • Ryan, R., Mitrovic, A., Vandenberg, R. (2004). The Chloride Permeation Pathway Of A Glutamate Transporter And Its Proximity To The Glutamate Translocation Pathway. Journal of Biological Chemistry, 279(20), 20742-20751.

2003

  • Ryan, R., Mitrovic, A., Vandenberg, R. (2003). Site-directed mutagenesis in the study of membrane transportes. In Qing Yan (Eds.), Membrane Transporters: Methods and Protocols (Methods in Molecular Biology Vol. 227), (pp. 97-108). New Jersey, USA: Humana Press.

2002

  • Ryan, R., Vandenberg, R. (2002). Distinct conformational states mediate the transport and anion channel properties of the glutamate transporter EAAT-1. Journal of Biological Chemistry, 277(16), 13494-13500.
  • Borgland, S., Connor, M., Ryan, R., Ball, H., Christie, M. (2002). Prostaglandin E2 inhibits calcium current in two sub-populations of acutely isolated mouse trigeminal sensory neurons. The Journal of Physiology, 539(2), 433-444.

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