Dr Jacky Stoeckli

Group Leader - Molecular Cell Biology
Senior Research Fellow
School of Life and Environmental Sciences

Member of the Charles Perkins Centre

D17 - Charles Perkins Centre
The University of Sydney

Telephone 0286271986

Biographical details

I obtained my PhD from the University of Basel in 2003. During this period I was trained in Cell Biology, Molecular Biology and Biochemistry and studied protein trafficking and trafficking signals. In 2004 I joined Prof David James' group when he was at the Garvan Institute in Sydney. Initially I focused on insulin regulated protein trafficking with a particular interest in GLUT4 and the vesicle trafficking proteins, such as Rabs and RabGAPs. In the James Lab I learned about Insulin Signalling, Glucose Metabolism and Mouse Physiology. In 2015 I was appointed as a Research Fellow at the Charles Perkins Centre and the School of Life and Environmental Sciences at the University of Sydney. My group "Molecular Cell Biology" within the larger James lab focuses on the insulin regulation of lipolysis.

Research interests

Adipose tissue lipolysis is induced during fasting via catecholamines and other fac­tors to activate lipolysis to release FFA into the circula­tion as an energy source for other tissues.These stimuli activate lipo­lysis in fat cells via elevated cAMP and acti­vation of PKA. Active PKA phosphorylates substrates like Hor­mone Sen­sitive Lipase (HSL) and the lipid droplet (LD) protein Perilipin 1 (PLIN1), releasing PLIN1 from CGI-58, leading to forma­tion of an active complex comprising Adipose Triglyc­er­ide Lipase (ATGL) and its co-activator CGI-58. This results in LD recruitment/acti­va­tion of the two lipases involved in the first two steps of lipolysis and complete hydro­lysis of TG to three molecules of FFAs and one of glycerol.

Insulin suppresses lipolysis but recently gaps have emerged in our understanding of insulin regulation of lipolysis. In view of the detrimental consequences of unsuppressed lipolysis, such as insulin resistance and fatty liver disease, it is surprising how little we know about the mechanism of this process. My focus is to shed light on this process and to identify novel regulators of lipolysis.

Teaching and supervision

Former UNSW PhD students:

Poh Sim Khoo (UNSW, graduated 2011)
Dave Pederson (graduated 2012)
Sean Humphrey (graduated 2013)
Sophie Trefely (graduated 2015)
Annabel Minard (graduated 2017)
Beverley Murrow (graduated 2017)

Former USYD Honours students (all 1st class Honours):

Anagha Killedar (2012)
Imogen Faulds (2015)
Gaia Herrmann (2016)

Current research students

Project title Research student
Delineating the mechanism by which insulin regulates lipolysis Armella ZADOORIAN

Current projects

Insulin regulation of lipolysis

Novel regulators of lipolysis

Selected grants

2018

  • Identification and Characterisation of a Novel Metabolite Involved in Insulin Resistance; Stoeckli J; Diabetes Australia/Diabetes Research Grant Program.

2017

  • Dissecting Rapamycin sensitive and insensitive effects of mTOR; James D, Stoeckli J, Yang J; National Health and Medical Research Council (NHMRC)/Project Grants.

2014

  • Mechanistic studies of insulin action; Stoeckli J, James D; National Health and Medical Research Council (NHMRC)/Project Grants.

2013

  • Defining how insulin regulates glucose uptake into muscle and fat, one of the major steps that is defective in Type 2 diabetes; James D, Stoeckli J, Lambright D, Hughes W; National Health and Medical Research Council (NHMRC)/Project Grants.

Selected publications

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Journals

  • Burchfield, J., Kebede, M., Meoli, C., Stoeckli, J., Whitworth, P., Wright, A., Hoffman, N., Minard, A., Ma, X., Krycer, J., Healy, M., Yau, B., Thomas, K., Cooney, G., James, D., Fazakerley, D., et al (2018). High dietary fat and sucrose results in an extensive and time-dependent deterioration in health of multiple physiological systems in mice. Journal of Biological Chemistry, 293(15), 5731-5745. [More Information]
  • Fazakerley, D., Minard, A., Krycer, J., Thomas, K., Stoeckli, J., Harney, D., Burchfield, J., Maghzal, G., Caldwell, S., Hartley, R., James, D., et al (2018). Mitochondrial oxidative stress causes insulin resistance without disrupting oxidative phosphorylation. Journal of Biological Chemistry, 293(19), 7315-7328. [More Information]
  • Su, Z., Deshpande, V., James, D., Stoeckli, J. (2018). Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating the stability of GLUT4 vesicle proteins. Journal of Biological Chemistry, 293(22), 8578-8587. [More Information]
  • Stoeckli, J., Fisher-Wellman, K., Chaudhuri, R., Zeng, X., Fazakerley, D., Meoli, C., Thomas, K., Hoffman, N., Mangiafico, S., Xirouchaki, C., Cooney, G., James, D., et al (2017). Metabolomic analysis of insulin resistance across different mouse strains and diets. Journal of Biological Chemistry, 292(47), 19135-19145. [More Information]
  • Li, Z., Schulze, R., Weller, S., Krueger, E., Schott, M., Zhang, X., Casey, C., Liu, J., Stoeckli, J., James, D., et al (2016). A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Science Advances, 2(12), 1-16. [More Information]
  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Coster, A., Stoeckli, J., James, D. (2016). Hyperactivation of the Insulin Signaling Pathway Improves Intracellular Proteostasis by Coordinately Up-regulating the Proteostatic Machinery in Adipocytes. Journal of Biological Chemistry, 291(49), 25629-25640. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stoeckli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17(1), 29-36. [More Information]
  • Stoeckli, J., Piper, R., James, D. (2016). Regulated versus Constitutive Secretion - A Major Form of Intercellular Communication. Encyclopedia of Cell Biology, 2, 376-383. [More Information]
  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • Trefely, S., Khoo, P., Krycer, J., Chaudhuri, R., Fazakerley, D., Parker, B., Sultani, G., Lee, J., Stephan, J., Torres, E., James, D., Stoeckli, J., et al (2015). Kinome Screen Identifies PFKFB3 and Glucose Metabolism as Important Regulators of the Insulin/IGF-1 Signalling Pathway. Journal of Biological Chemistry, 290(43), 25834-25846. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Fazakerley, D., Naghiloo, S., Chaudhuri, R., Koumanov, F., Burchfield, J., Thomas, K., Krycer, J., Prior, M., Parker, B., Murrow, B., Stoeckli, J., Meoli, C., James, D., et al (2015). Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumour Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin Action in Adipocytes. Journal of Biological Chemistry, 290(39), 23528-23542. [More Information]
  • Tan, S., Fisher_Wellman, K., Fazakerley, D., Ng, Y., Pant, H., Li, J., Meoli, C., Coster, A., Stoeckli, J., James, D. (2015). Selective insulin resistance in adipocytes. Journal of Biological Chemistry, 290(18), 11337-11348. [More Information]
  • Stoeckli, J., Meoli, C., Hoffman, N., Fazakerley, D., Pant, H., Cleasby, M., Ma, X., Kleinert, M., Brandon, A., Lopez, J., James, D., et al (2015). The RabGAP TBC1D1 Plays a Central Role in Exercise-Regulated Glucose Metabolism in Skeletal Muscle. Diabetes, 64(6), 1914-1922. [More Information]

2018

  • Burchfield, J., Kebede, M., Meoli, C., Stoeckli, J., Whitworth, P., Wright, A., Hoffman, N., Minard, A., Ma, X., Krycer, J., Healy, M., Yau, B., Thomas, K., Cooney, G., James, D., Fazakerley, D., et al (2018). High dietary fat and sucrose results in an extensive and time-dependent deterioration in health of multiple physiological systems in mice. Journal of Biological Chemistry, 293(15), 5731-5745. [More Information]
  • Fazakerley, D., Minard, A., Krycer, J., Thomas, K., Stoeckli, J., Harney, D., Burchfield, J., Maghzal, G., Caldwell, S., Hartley, R., James, D., et al (2018). Mitochondrial oxidative stress causes insulin resistance without disrupting oxidative phosphorylation. Journal of Biological Chemistry, 293(19), 7315-7328. [More Information]
  • Su, Z., Deshpande, V., James, D., Stoeckli, J. (2018). Tankyrase modulates insulin sensitivity in skeletal muscle cells by regulating the stability of GLUT4 vesicle proteins. Journal of Biological Chemistry, 293(22), 8578-8587. [More Information]

2017

  • Stoeckli, J., Fisher-Wellman, K., Chaudhuri, R., Zeng, X., Fazakerley, D., Meoli, C., Thomas, K., Hoffman, N., Mangiafico, S., Xirouchaki, C., Cooney, G., James, D., et al (2017). Metabolomic analysis of insulin resistance across different mouse strains and diets. Journal of Biological Chemistry, 292(47), 19135-19145. [More Information]

2016

  • Li, Z., Schulze, R., Weller, S., Krueger, E., Schott, M., Zhang, X., Casey, C., Liu, J., Stoeckli, J., James, D., et al (2016). A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Science Advances, 2(12), 1-16. [More Information]
  • Minard, A., Wong, M., Chaudhuri, R., Tan, S., Humphrey, S., Parker, B., Yang, J., Laybutt, D., Cooney, G., Coster, A., Stoeckli, J., James, D. (2016). Hyperactivation of the Insulin Signaling Pathway Improves Intracellular Proteostasis by Coordinately Up-regulating the Proteostatic Machinery in Adipocytes. Journal of Biological Chemistry, 291(49), 25629-25640. [More Information]
  • Minard, A., Tan, S., Yang, P., Fazakerley, D., Domanova, W., Parker, B., Humphrey, S., Jothi, R., Stoeckli, J., James, D. (2016). mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Reports, 17(1), 29-36. [More Information]
  • Stoeckli, J., Piper, R., James, D. (2016). Regulated versus Constitutive Secretion - A Major Form of Intercellular Communication. Encyclopedia of Cell Biology, 2, 376-383. [More Information]

2015

  • Hoffman, N., Parker, B., Chaudhuri, R., Fisher-Wellman, K., Kleinert, M., Humphrey, S., Yang, P., Holliday, M., Trefely, S., Fazakerley, D., Stoeckli, J., Burchfield, J., James, D., et al (2015). Global Phosphoproteomic Analysis of Human Skeletal Muscle Reveals a Network of Exercise-Regulated Kinases and AMPK Substrates. Cell Metabolism, 22(5), 922-935. [More Information]
  • Trefely, S., Khoo, P., Krycer, J., Chaudhuri, R., Fazakerley, D., Parker, B., Sultani, G., Lee, J., Stephan, J., Torres, E., James, D., Stoeckli, J., et al (2015). Kinome Screen Identifies PFKFB3 and Glucose Metabolism as Important Regulators of the Insulin/IGF-1 Signalling Pathway. Journal of Biological Chemistry, 290(43), 25834-25846. [More Information]
  • Chaudhuri, R., Sadrieh, A., Hoffman, N., Parker, B., Humphrey, S., Stoeckli, J., Hill, A., James, D., Yang, J. (2015). PhosphOrtholog: a web-based tool for cross-species mapping of orthologous protein post-translational modification. BMC Genomics, 16(1), 1-14. [More Information]
  • Fazakerley, D., Naghiloo, S., Chaudhuri, R., Koumanov, F., Burchfield, J., Thomas, K., Krycer, J., Prior, M., Parker, B., Murrow, B., Stoeckli, J., Meoli, C., James, D., et al (2015). Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumour Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin Action in Adipocytes. Journal of Biological Chemistry, 290(39), 23528-23542. [More Information]
  • Tan, S., Fisher_Wellman, K., Fazakerley, D., Ng, Y., Pant, H., Li, J., Meoli, C., Coster, A., Stoeckli, J., James, D. (2015). Selective insulin resistance in adipocytes. Journal of Biological Chemistry, 290(18), 11337-11348. [More Information]
  • Stoeckli, J., Meoli, C., Hoffman, N., Fazakerley, D., Pant, H., Cleasby, M., Ma, X., Kleinert, M., Brandon, A., Lopez, J., James, D., et al (2015). The RabGAP TBC1D1 Plays a Central Role in Exercise-Regulated Glucose Metabolism in Skeletal Muscle. Diabetes, 64(6), 1914-1922. [More Information]

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