People_

Associate Professor Ronald Clarke

BSc PhD Adelaide

Associate Professor

Phone

61 2 9351 4406

Fax

61 2 9351 3329

Email

ronald.clarke@sydney.edu.au

Address

F11 - Chemistry Building

The University of Sydney

Details

Member of The University of Sydney Nano Institute

Websites

Contact Details

Biographical details

BSc (Hons I) 1981, PhD 1986, University of Adelaide
Humboldt Fellow, University of Constance, 1987-88
Leverhulme Fellow, University of East Anglia, 1989
Max Planck Research Fellow, Liebig Fellow, Fritz Haber Institute, Berlin, 1990-94
Dr. Habil. (Physical Chemistry), 1995, Free University of Berlin
Max Planck Research Fellow, Max Planck Institute for Biophysics, Frankfurt/Main 1995-99
Dr. Habil. (physical chemistry) 1999, Johann Wolfgang von Goethe University, Frankfurt/Main
University of Sydney; Lecturer 1999-2002
University of Sydney; Senior Lecturer 2003 - 2012
University of Sydney, Associate Professor 2013 -
Dozor Visiting Professor, Ben-Gurion University of the Negev, Beer-Sheva, Israel, 2008
McAulay-Hope Prize for Original Biophysics (Australian Society for Biophysics), 2010
Visiting Professor, University of Science and Technology Hanoi, Vietnam, 2011 -
Research Affiliate, Kolling Institute of Medical Research, Sydney
Humboldt Fellow, Technical University Berlin, 2014-2015

Research interests

Biophysical chemistry
Biological membranes and membrane proteins
Fluorescence spectroscopy
Rapid reaction kinetics
Regulation and mechanism of ion transport across biological membranes
Voltage-sensitive fluorescent dyes
Na+,K+-ATPase
Membrane electrical properties

Ion-transporting Membrane Proteins: Ion-transporting membrane proteins play a decisive role in the metabolism of all cells and in numerous physiological processes, e.g., ATP production, nerve impulse propagation, and muscle contraction. A deeper understanding of their mechanisms and regulation can be obtained by the determination of the kinetics of their individual reaction steps. One approach to this research goal is to make use of the electrical current they generate across the cell membrane and to apply electrophysiological methods, such as the patch-clamp technique. Another is to convert the electrical voltage that the proteins produce into an optical signal by incorporating a voltage-sensitive dye into the membrane. Voltage-sensitive styrylpyridinium dyes allow a rapid detection (within nanoseconds) of local changes in electrical field strength within membranes. The great advantage of the dyes is that they allow one to investigate electrogenic reaction steps of membrane proteins in open membrane fragments, i.e. conditions under which electrophysiological methods are not applicable.

Na⁺,K⁺-ATPase: The Na⁺,K⁺-ATPase is the enzyme responsible for maintaining the physiological essential Na⁺ and K⁺concentration gradients across the plasma membrane of all animal cells. Jens-Christian Skou (University of Aarhus, Denmark) was awarded the Nobel Prize for Chemistry for its discovery in 1997. Using the voltage-sensitive probe RH421 in conjunction with the fluorescence stopped-flow method we have investigated in detail the enzyme’s kinetics and mechanism. This has allowed us to determine rate constants for most of the steps of the enzyme’s reaction cycle and to locate the rate-determining steps. The reaction cycle of the Na⁺,K⁺-ATPase is universally described in biology and chemistry textbooks by the Albers-Post model, which represents the catalytic unit of the enzyme as a monomer undergoing a sequence of ion-binding, conformational changes and ATP phosphorylation/dephosphorylation steps. Recently we have discovered that this description is inadequate and, based on our experimental results, have proposed a new model, in which the enzyme exists in dimeric form with two gears of ion pumping depending on the number of ATP molecules bound. We are currently investigating further the mechanism by which the two gears of pumping come about.

Membrane Dipole Potential and Orientational Polarisability: Ion-transporting membrane proteins, such as the Na⁺,K⁺-ATPase, are very sensitive to the composition of their lipid surroundings. The mechanisms by which lipids and membrane proteins interact are still unclear. One possibility which we are investigating is via an electrostatic interaction between charged or dipolar groups on both the lipid and the protein. Within the head-group region of phospholipid membranes there exists an electrical potential (the dipole potential) of ca. 200-400 mV, positive in the membrane interior. This produces a very large electric field strength of around 10⁹ V m-1 within the membrane, which could potentially influence the energy of intermediate states in the pumping cycle of ion-transporting membrane proteins, thus changing the activation energies of steps of the cycle and hence their rate constants. However, another important consideration is the degree to which charged groups of the lipid are free to reorientate around the intermediate states of ion pumps, which is determined by the order or the fluidity of the membrane. The effects of the dipole potential and membrane fluidity are combined in the concept of the membrane orientational polarisability, for which we recently developed a fluorescence-based method of determination using the probe di-8-ANEPPS. Future work will involve analysing for a correlation between orientational polarisability and ion pump activity.

Fluorescent Styrylpyridinium Voltage-Sensitive Membrane Probes: Voltage-sensitive membrane probes based on the styrylpyridinium fluorophore have proven to be very useful in the neurosciences for the optical imaging of voltage transients of neurons and in biophysical research for the investigation of the kinetics of ion-transporting membrane proteins. However, in spite of the successes which have been achieved through their use, they suffer from the disadvantage that they are often photochemically unstable and phototoxic. In order to develop improved probes it is necessary to obtain fundamental knowledge on the origin of the dyes’ photochemistry. We have been investigating in particular the probe RH421, which displays the unusual property of undergoing an increase in its fluorescence on excitation when bound to lipid membranes, and the more recently developed probe ANNINE 5.

Selected publications

Pumps, Channels, and Transporters: Methods of Functional Analysis

(John Wiley & Sons, 2015)

Publications

By Type

Expand all

Edited Books

Clarke, R., Khalid, M. (2015). Pumps, Channels, and Transporters: Methods of Functional Analysis. Hoboken: John Wiley & Sons. [More Information]

Book Chapters

Clarke, R. (2019). Effect of Cholesterol on the Dipole Potential of Lipid Membranes. In Avia Rosenhouse-Dantsker and Anna N. Bukiya (Eds.), Cholesterol Modulation of Protein Function: Sterol Specificity and Indirect Mechanisms, (pp. 135-154). Cham: Springer. [More Information]
Khalid, M., Clarke, R. (2015). Introduction. In Ronald J. Clarke, Mohammed A.A. Khalid (Eds.), Pumps, Channels, and Transporters: Methods of Functional Analysis, (pp. 1-22). Hoboken: John Wiley & Sons. [More Information]
Clarke, R., Khalid, M. (2015). Stopped-flow fluorimetry using voltage-sensitive fluorescent membrane probes. In Ronald J. Clarke, Mohammed A.A. Khalid (Eds.), Pumps, Channels, and Transporters: Methods of Functional Analysis, (pp. 179-209). Hoboken: John Wiley & Sons. [More Information]
Clarke, R. (2010). Electric Field Sensitive Dyes. In Alexander P Demchenko (Eds.), Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design, (pp. 331-344). Berlin Heidelberg: Springer. [More Information]
Clarke, R. (2009). Probing Kinetics of Ion Pumps via Voltage-Sensitive Fluorescent Dyes. In Ewa M Goldys (Eds.), Fluorescence Applications in Biotechnology and the Life Sciences, (pp. 349-363). New Jersey, USA: John Wiley & Sons.

show 2 more

Journals

Lev, B., Vorobyov, I., Clarke, R., Allen, T. (2024). The Membrane Dipole Potential and the Roles of Interfacial Water and Lipid Hydrocarbon Chains. The Journal of Physical Chemistry B. [More Information]
Blayney, E., Chennath, M., Cranfield, C., Clarke, R. (2023). Bioinformatic Analysis of Na+, K+-ATPase Regulation through Phosphorylation of the Alpha-Subunit N-Terminus. International Journal of Molecular Sciences, 24(1). [More Information]
Clarke, R. (2023). Electrostatic switch mechanisms of membrane protein trafficking and regulation. Biophysical Reviews, 15(6), 1967-1985. [More Information]
Lev, B., Chennath, M., Cranfield, C., Cornelius, F., Allen, T., Clarke, R. (2023). Involvement of the alpha-subunit N-terminus in the mechanism of the Na+,K+-ATPase. Biochimica et Biophysica Acta, 1870 (7), 119539. [More Information]
Clarke, R. (2023). SUSTAINABILITY FROM A CELL PERSPECTIVE. Proceedings of the Royal Society of Victoria, 135(2), 69-71. [More Information]
Catauro, M., Blanco, I., Clarke, R., Fiorentino, M., D'Angelo, A., Raffaini, G. (2022). Sol–Gel Synthesis, Thermal Characterization, Surface Interactions, and Release Kinetics of Silica/Drug Hybrid System. Macromolecular Symposia, 404(1). [More Information]
Catauro, M., D’errico, Y., D'angelo, A., Clarke, R., Blanco, I. (2021). Antibacterial activity and iron release of organic-inorganic hybrid biomaterials synthesized via the sol-gel route. Applied Sciences, 11(19), 9311. [More Information]
Clarke, R. (2021). Biological Membrane Asymmetry and its Role in Bone Mineralization. Macromolecular Symposia, 396(1), 1-3. [More Information]
Wang, T., Sabatini, R., Chan, B., Hou, J., Huynh, V., Proschogo, N., Xie, Z., Wang, T., Gao, L., Zhang, J., Hawkett, B., Clarke, R., Kepert, C., Lakhwani, G., D'Alessandro, D., et al (2021). Fluorescence Enhancement through Confined Oligomerization in Nanochannels: An Anthryl Oligomer in a Metal-Organic Framework. ACS Materials Letters, 3(11), 1599-1604. [More Information]
Hossain, K., Clayton, D., Goodchild, S., Rodger, A., Payne, R., Cornelius, F., Clarke, R. (2021). Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases. Faraday Discussions, 232, 172-187. [More Information]
Bryant, S., Garcia, A., Clarke, R., Warr, G. (2021). Selective ion transport across a lipid bilayer in a protic ionic liquid. Soft Matter, 17(10), 2688-2694. [More Information]
Yasir, M., Dutta, D., Hossain, K., Chen, R., Ho, K., Kuppusamy, R., Clarke, R., Kumar, N., Wilcox, M. (2020). Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa. Frontiers in Microbiology, 10, 3053. [More Information]
Clarke, R. (2020). One sequence, one structure: Demise of a dogma, or fake news. Journal and Proceedings of the Royal Society of New South Wales, 152(3), 343-345. [More Information]
Gorman, A., Hossain, K., Cornelius, F., Clarke, R. (2020). Penetration of phospholipid membranes by poly-L-lysine depends on cholesterol and phospholipid composition. Biochimica et Biophysica Acta - Biomembranes, 1862 (2), 183128. [More Information]
Chisholm, T., Kulkarni, S., Hossain, K., Cornelius, F., Clarke, R., Payne, R. (2020). Peptide Ligation at High Dilution via Reductive Diselenide-Selenoester Ligation. Journal of the American Chemical Society, 142(2), 1090-1100. [More Information]
Clarke, R., Hossain, K., Cao, K. (2020). Physiological roles of transverse lipid asymmetry of animal membranes. Biochimica et Biophysica Acta - Biomembranes, 1862 (10), 183382. [More Information]
Hossain, K., Li, X., Zhang, T., Paula, S., Cornelius, F., Clarke, R. (2020). Polarity of the ATP binding site of the Na+,K+-ATPase, gastric H+,K+-ATPase and sarcoplasmic reticulum Ca2+-ATPase. Biochimica et Biophysica Acta - Biomembranes, 1862 (2), 183138. [More Information]
Garcia, A., Lev, B., Hossain, K., Gorman, A., Diaz, D., Pham, T., Cornelius, F., Allen, T., Clarke, R. (2019). Cholesterol depletion inhibits Na+,K+-ATPase activity in a near-native membrane environment. Journal of Biological Chemistry, 294(15), 5956-5969. [More Information]
Garcia, A., Clarke, R. (2019). Evidence for ATP interaction with phosphatidylcholine bilayers. Biophysical Journal, 116(3 (S1)), 229a. [More Information]
Garcia, A., Pochinda, S., Elgaard-Jørgensen, P., Khandelia, H., Clarke, R. (2019). Evidence for ATP Interaction with Phosphatidylcholine Bilayers. Langmuir, 35(30), 9944-9953. [More Information]
Hossain, K., Clarke, R. (2019). General and specific interactions of the phospholipid bilayer with P-type ATPases. Biophysical Reviews, 11(3), 353-364. [More Information]
Garcia, A., Zou, H., Hossain, K., Xu, Q., Buda, A., Clarke, R. (2019). Polar interactions play an important role in the energetics of the main phase transition of phosphatidylcholine membranes. ACS Omega, 4(1), 518-527. [More Information]
Diaz, D., Clarke, R. (2018). Evolutionary analysis of the lysine-rich N-terminal cytoplasmic domains of the gastric H+,K+-ATPase and the Na+,K+-ATPase. Journal of Membrane Biology, 251(5-6), 653-666. [More Information]
Nguyen, K., Garcia, A., Sani, M., Diaz, D., Dubey, V., Clayton, D., Dal Poggetto, G., Cornelius, F., Payne, R., Separovic, F., Clarke, R., et al (2018). Interaction of N-terminal peptide analogues of the Na+, K+ -ATPase with membranes. Biochimica et Biophysica Acta - Biomembranes, 1860 (6), 1282-1291. [More Information]
Vleeskens, E., Clarke, R. (2018). Kinetic contribution to extracellular Na+/K+ selectivity in the Na+/K+ pump. FEBS Open Bio, 8(5), 854-859. [More Information]
Jiang, Q., Garcia, A., Han, M., Cornelius, F., Apell, H., Khandelia, H., Clarke, R. (2017). Electrostatic stabilization plays a central role in autoinhibitory regulation of the Na+, K+ - ATPase. Biophysical Journal, 112(2), 288-299. [More Information]
Garcia, A., Pratap, P., Lupfert, C., Cornelius, F., Jacquemin, D., Lev, B., Allen, T., Clarke, R. (2017). The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane surface. Biochimica et Biophysica Acta - Biomembranes, 1859 (5), 813-823. [More Information]
Garcia, A., Liu, C., Cornelius, F., Clarke, R., Rasmussen, H. (2016). Glutathionylation-dependence of Na+ -K+ -pump currents can mimic reduced subsarcolemmal Na+ diffusion. Biophysical Journal, 110(5), 1099-1109. [More Information]
Lambropoulos, N., Garcia, A., Clarke, R. (2016). Stimulation of Na+,K+-ATPase Activity as a Possible Driving Force in Cholesterol Evolution. Journal of Membrane Biology, 249(3), 251-259. [More Information]
Garcia, A., Rasmussen, H., Clarke, R. (2015). A novel kinetic model based on the Albers-Post scheme that mimics the behaviour of the Na+, K+-ATPase in situ. Heart, Lung, and Circulation, 24(S3), S224-S224. [More Information]
Clarke, R. (2015). Dipole-potential-mediated effects on ion pump kinetics. Biophysical Journal, 109(8), 1513-1520. [More Information]
Begum, S., Ratna, S., Clarke, R., Ahmed, M. (2015). Excess molar volumes, refractive indices and transport properties of aqueous solutions of poly(ethylene glycol)s at (303.15-323.15) K. Journal of Molecular Liquids, 202, 176-188. [More Information]
Garcia, A., Eljack, N., Sani, M., Separovic, F., Rasmussen, H., Kopec, W., Khandelia, H., Cornelius, F., Clarke, R. (2015). Membrane accessibility of glutathione. Biochimica et Biophysica Acta - Biomembranes, 1848 (10 (Part A)), 2430-2436. [More Information]
Lev, B., Clarke, R., Allen, T. (2014). Effects of lipid composition on biological membrane electrostatics. Biophysical Journal, 106(2, S1), 80a-80a. [More Information]
Mares, J., Garcia, A., Rasmussen, H., Cornelius, F., Mahmmoud, Y., Berlin, J., Lev, B., Allen, T., Clarke, R. (2014). Identification of Electric-Field-Dependent Steps in the Na+,K+ -Pump Cycle. Biophysical Journal, 107(6), 1352-1363. [More Information]
Eljack, N., Ma, H., Drucker, J., Shen, C., Hambley, T., New, E., Friedrich, T., Clarke, R. (2014). Mechanisms of cell uptake and toxicity of the anticancer drug cisplatin. Metallomics, 6(11), 2126-2133. [More Information]
Khalid, M., Suliman, R., Ahmed, R., Salim, H., Clarke, R. (2014). The high and low affinity binding sites of digitalis glycosides to Na,K-ATPase. Arabian Journal for Science and Engineering, 39(1), 75-85. [More Information]
Garcia, A., Fry, N., Karimi, K., Liu, C., Apell, H., Rasmussen, H., Clarke, R. (2013). Extracellular allosteric Na+ binding to the Na+, K+-ATPase in cardiac myocytes. Biophysical Journal, 105(12), 2695-2705. [More Information]
Clarke, R., Catauro, M., Rasmussen, H., Apell, H. (2013). Quantitative calculation of the role of the Na+ ,K+ -ATPase in thermogenesis. Biochimica et Biophysica Acta, 1827 (10), 1205-1212. [More Information]
Liu, C., Fry, N., Hamilton, E., Chia, K., Garcia, A., Karimigalougahi, K., Figtree, G., Clarke, R., Bundgaard, H., Rasmussen, H. (2013). Redox-dependent regulation of the Na+ -K+ pump: New twists to an old target for treatment of heart failure. Journal of Molecular and Cellular Cardiology, 61, 94-101. [More Information]
Ching, V., Clarke, R., Rendina, L. (2013). Supramolecular beta-cyclodextrin adducts of boron-rich DNA metallointercalators containing dicarba-closo-dodecaborane(12). Inorganic Chemistry, 52(18), 10356-10367. [More Information]
Begum, S., Clarke, R., Ahmed, M., Begum, S., Saleh, M. (2013). Volumetric, viscosimetric and surface properties of aqueous solutions of triethylene glycol, tetraethylene glycol, and tetraethylene glycol dimethyl ether. Journal of Molecular Liquids, 177, 11-18. [More Information]
Trevitt, A., Reimers, J., Clarke, R., Vandenberg, J. (2012). BIOPHYSCHEM2011: A joint meeting of the Australian Society for Biophysics and the RACI Chemistry Division. Australian Journal of Chemistry, 65(5), 439-441. [More Information]
Yin, Y., Fisher, K., Nosworthy, N., Bax, D., Clarke, R., McKenzie, D., Bilek, M. (2012). Comparison on protein adsorption properties of diamond-like carbon and nitrogen-containing plasma polymer surfaces. Thin Solid Films, 520(7), 3021-3025. [More Information]
Garcia, A., Rasmussen, H., Apell, H., Clarke, R. (2012). Kinetic comparisons of heart and kidney Na+, K+ -ATPases. Biophysical Journal, 103(4), 677-688. [More Information]
Liu, C., Garcia, A., Mahmmoud, Y., Hamilton, E., Galougahi, K., Fry, N., Figtree, G., Cornelius, F., Clarke, R., Rasmussen, H. (2012). Susceptibility of Î²1 Na+ -K+ pump subunit to glutathionylation and oxidative inhibition depends on conformational state of pump. Journal of Biological Chemistry, 287(15), 12353-12364. [More Information]
Ching, V., Clifford, S., Bhadbhade, M., Clarke, R., Rendina, L. (2012). Synthesis and supramolecular studies of chiral boronated platinum(II) complexes: Insights into the molecular recognition of carboranes by beta-cyclodextrin. Chemistry - A European Journal, 18(45), 14413-14425. [More Information]
Clarke, R. (2011). A perspective on biophysical chemistry. Australian Journal of Chemistry, 64(1), 3-4. [More Information]
Begum, S., Clarke, R., Ahmed, M., Begum, S., Saleh, M. (2011). Densities, Viscosities, and surface Tensions of the System water + Diethylene Glycol. Journal of Chemical and Engineering Data, 56(2), 303-306. [More Information]
Meyers, S., Cornelius, F., Apell, H., Clarke, R. (2011). Kinetics of K+ Occlusion by the Phosphoenzyme of the Na+,K+-ATPase. Biophysical Journal, 100(1), 70-79. [More Information]
Pisani, M., Fromm, P., Mulyana, Y., Clarke, R., Korner, H., Heimann, K., Collins, J., Keene, F. (2011). Mechanism of cytotoxicity and cellular uptake of lipophilic inert dinuclear polypyridylruthenium(II) complexes. ChemMedChem: chemistry enabling drug discovery, 6(5), 848-858. [More Information]
Clarke, R., Fan, X. (2011). Pumping ions. Clinical and Experimental Pharmacology and Physiology, 38(11), 726-733. [More Information]
Clarke, R. (2011). Rapid Reaction Kinetics: Lessons Learnt from Ion Pumps. Australian Journal of Chemistry, 64(1), 5-8. [More Information]
Hosseini, S., Bhadbhade, M., Clarke, R., Rutledge, P., Rendina, L. (2011). Synthesis, carbohydrate- and DNA-binding studies of cationic 2,2':6',2"-terpyridineplatinum(II) complexes containing N- and S-donor boronic acid ligands. Dalton Transactions, 40(2), 506-513. [More Information]
Khalid, M., Cornelius, F., Clarke, R. (2010). Dual Mechanisms of Allosteric Acceleration of the Na+,K+-ATPase by ATP. Biophysical Journal, 98(10), 2290-2298. [More Information]
Amoroso, S., Clarke, R., Larkum, A., Quinnell, R. (2010). Electrogenic plasma membrane H+-ATPase activity using voltage sensitive dyes. Journal of Bioenergetics and Biomembranes, 42(5), 387-393. [More Information]
Khalid, M., Fouassier, G., Apell, H., Cornelius, F., Clarke, R. (2010). Interaction of ATP with the phosphoenzyme of the Na+,K +-ATPase. Biochemistry, 49(6), 1248-1258. [More Information]
Noske, R., Cornelius, F., Clarke, R. (2010). Investigation of the enzymatic activity of the Na+,K+-ATPase via isothermal titration microcalorimetry. Biochimica et Biophysica Acta, 1797 (8), 1540-1545. [More Information]
Ash, M., Guilfoyle, A., Clarke, R., Guss, M., Maher, M., Jormakka, M. (2010). Potassium-activated GTPase reaction in the G protein-coupled ferrous iron transporter B. Journal of Biological Chemistry, 285(19), 14594-14602. [More Information]
Starke-Peterkovic, T., Clarke, R. (2009). Effect of headgroup on the dipole potential of phospholipid vesicles. European Biophysics Journal, 39(1), 103-110. [More Information]
Clarke, R. (2009). Mechanism of allosteric effects of ATP on the kinetics of P-type ATPases. European Biophysics Journal, 39(1), 3-17. [More Information]
Pilotelle-Bunner, A., Cornelius, F., Sebban, P., Kuchel, P., Clarke, R. (2009). Mechanism of Mg(2+) binding in the Na+,K+ -ATPase. Biophysical Journal, 96(9), 3753-3761. [More Information]
Guilfoyle, A., Maher, M., Rapp, M., Clarke, R., Harrop, S., Jormakka, M. (2009). Structural basis of GDP release and gating in G protein coupled Fe(2+) transport. EMBO Journal, 28(17), 2677-2685. [More Information]
Pilotelle-Bunner, A., Beaunier, P., Tandori, J., Maroti, P., Clarke, R., Sebban, P. (2009). The local electric field within phospholipid membranes modulates the charge transfer reactions in reaction centres. Biochimica et Biophysica Acta, 1787 (8), 1039-1049. [More Information]
Pilotelle-Bunner, A., Matthews, J., Cornelius, F., Apell, H., Sebban, P., Clarke, R. (2008). ATP Binding Equilibria of the Na(+),K(+)-ATPase. Biochemistry, 47(49), 13103-13114. [More Information]
Pham, T., Clarke, R. (2008). Solvent Dependence of the Photochemistry of the Styrylpyridinium Dye RH421. The Journal of Physical Chemistry B, 112(20), 6513-6520. [More Information]
Clarke, R., Apell, H., Kong, B. (2007). Allosteric effect of ATP on Na+,K+-ATPase conformational kinetics. Biochemistry, 46(23), 7034-7044. [More Information]
Hansen, P., Clarke, R., Buhagiar, K., Hamilton, E., Garcia, A., White, C., Rasmussen, H. (2007). Alloxan-induced diabetes reduces sarcolemmal Na+-K+ pump function in rabbit ventricular myocytes. American Journal of Physiology: Cell Physiology, 292(0), C1070-C1077. [More Information]
Vitha, M., Clarke, R. (2007). Comparison of excitation and emission ratiometric fluorescence methods for quantifying the membrane dipole potential. Biochimica et Biophysica Acta - Biomembranes, 1768 (0), 107-114. [More Information]
Le Goff, G., Vitha, M., Clarke, R. (2007). Orientational polarisability of lipid membrane surfaces. Biochimica et Biophysica Acta, 1768 (0), 562-570. [More Information]
Clarke, R., Kane, D. (2007). Two gears of pumping by the sodium pump. Biophysical Journal, 93(12), 4187-4196. [More Information]
Starke-Peterkovic, T., Turner, N., Vitha, M., Waller, M., Hibbs, D., Clarke, R. (2006). Cholesterol effect on the dipole potential of lipid membranes. Biophysical Journal, 90(11), 4060-4070. [More Information]
Amoroso, S., Agon, V., Starke-Peterkovic, T., McLeod, M., Apell, H., Sebban, P., Clarke, R. (2006). Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive styrylpyridinium fluorescent membrane probes. Photochemistry and Photobiology, 82(2), 495-502. [More Information]
Starke-Peterkovic, T., Clarke, R., Turner, N., Else, P. (2005). Electric field strength of membrane lipids from vertebrate species: membrane lipid composition and Na+-K+-ATPase molecular activity. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 288(3), R663-R670. [More Information]
William, M., Vien, J., Hamilton, E., Garcia, A., Bundgaard, H., Clarke, R., Rasmussen, H. (2005). The nitric oxide donor sodium nitroprusside stimulates the Na+-K+ pump in isolated rabbit cardiac myocytes. Journal of Physiology, 565.3(3), 815-825. [More Information]
Buhagiar, K., Hansen, P., Kong, B., Clarke, R., Fernandes, C., Rasmussen, H. (2004). Dietary Cholesterol Alters Na+/K+ Selectivity At Intracellular Na+/K+ Pump Sites In Cardiac Myocytes. American Journal of Physiology: Cell Physiology, 286(2), C398-C405.
Clarke, R., Oprysa, A. (2004). Fluorescence And Light Scattering. Journal of Chemical Education, 81(5), 705-707.
Kong, B., Clarke, R. (2004). Identification Of Potential Regulatory Sites Of The Na+,K+-Atpase By Kinetic Analysis. Biochemistry, 43(8), 2241-2250. [More Information]
Nieuwenburg, P., Clarke, R., Cai, Z., Chen, M., Larkum, A., Cabral, N., Ghiggino, K., Reimers, J. (2003). Examination of the photophysical processes of chlorophyll d leading to a clarificaiton of proposed uphill energy transfer processess in cells of Acaryochloris marina. Photochemistry and Photobiology, 77(6), 628-637. [More Information]
Wang, Y., Winnik, F., Clarke, R. (2003). Interaction between DMPC liposomes and HM-PNIPAM polymer. Biophysical Chemistry, 104(2), 449-458. [More Information]
Clarke, R., Humphrey, P., Lupfert, C., Apell, H., Cornelius, B. (2003). Kinetic investigations of the mechanism of the rate-determining step of the Na+, K+-ATPase pump cycle. Annals of the New York Academy of Sciences, 986, 159-162. [More Information]
Hansen, P., Buhagiar, K., Kong, B., Clarke, R., Gray, D., Rasmussen, H. (2002). Dependence of Na+-K+ pump current-voltage relationship on intracellular Na+, K+, and Cs+ in rabbit cardiac myocytes. American Journal of Physiology: Cell Physiology, 283(5), 1511-1521. [More Information]
Schamberger, J., Clarke, R. (2002). Hydrophobic Ion Hydration and the Magnitude of the Dipole Potential. Biophysical Journal, 82(June 2002), 3081-3088. [More Information]
Humphrey, P., Lupfert, C., Apell, H., Cornelius, F., Clarke, R. (2002). Mechanism of the Rate-Determining Step of the Na+,K+-ATPase Pump Cycle. Biochemistry, 41(30), 9496-9507. [More Information]
Clarke, R., Bamberg, E., Fendler, K. (2001). Electrogenic properties of the Na+, K+ -ATPase probed by presteady state and relaxation studies. Journal of Bioenergetics and Biomembranes, 33, 401-405.
Clarke, R., Frank, J., Vater, J., Holzwarth, J. (2001). Influence of allosteric effectors on the kinetics and equilibrium binding of phosphoenolpyruvate (PEP) to phosphoenolpyruvate carboxylase (PEPC) from Zea mays. Biophysical Chemistry, 92, 53-64. [More Information]
Lupfert, C., Grell, E., Pintschovius, V., Apell, H., Cornelius, B., Clarke, R. (2001). Rate limitation of the Na+, K+ -ATPase pump cycle. Biophysical Journal, 81(4), 2069-2081.
Clarke, R. (2001). The dipole potential of phospholipid membranes and methods for its detection. Advances in Colloid and Interface Science, 89-90, 263-281.

show 85 more

Magazine / Newspaper Articles

Clarke, R. (2013). When the going gets hot. Chemistry in Australia, June 2013.

By Year

Expand all

2024

Lev, B., Vorobyov, I., Clarke, R., Allen, T. (2024). The Membrane Dipole Potential and the Roles of Interfacial Water and Lipid Hydrocarbon Chains. The Journal of Physical Chemistry B. [More Information]

2023

Blayney, E., Chennath, M., Cranfield, C., Clarke, R. (2023). Bioinformatic Analysis of Na+, K+-ATPase Regulation through Phosphorylation of the Alpha-Subunit N-Terminus. International Journal of Molecular Sciences, 24(1). [More Information]
Clarke, R. (2023). Electrostatic switch mechanisms of membrane protein trafficking and regulation. Biophysical Reviews, 15(6), 1967-1985. [More Information]
Lev, B., Chennath, M., Cranfield, C., Cornelius, F., Allen, T., Clarke, R. (2023). Involvement of the alpha-subunit N-terminus in the mechanism of the Na+,K+-ATPase. Biochimica et Biophysica Acta, 1870 (7), 119539. [More Information]
Clarke, R. (2023). SUSTAINABILITY FROM A CELL PERSPECTIVE. Proceedings of the Royal Society of Victoria, 135(2), 69-71. [More Information]

show 1 more

2022

Catauro, M., Blanco, I., Clarke, R., Fiorentino, M., D'Angelo, A., Raffaini, G. (2022). Sol–Gel Synthesis, Thermal Characterization, Surface Interactions, and Release Kinetics of Silica/Drug Hybrid System. Macromolecular Symposia, 404(1). [More Information]

2021

Catauro, M., D’errico, Y., D'angelo, A., Clarke, R., Blanco, I. (2021). Antibacterial activity and iron release of organic-inorganic hybrid biomaterials synthesized via the sol-gel route. Applied Sciences, 11(19), 9311. [More Information]
Clarke, R. (2021). Biological Membrane Asymmetry and its Role in Bone Mineralization. Macromolecular Symposia, 396(1), 1-3. [More Information]
Wang, T., Sabatini, R., Chan, B., Hou, J., Huynh, V., Proschogo, N., Xie, Z., Wang, T., Gao, L., Zhang, J., Hawkett, B., Clarke, R., Kepert, C., Lakhwani, G., D'Alessandro, D., et al (2021). Fluorescence Enhancement through Confined Oligomerization in Nanochannels: An Anthryl Oligomer in a Metal-Organic Framework. ACS Materials Letters, 3(11), 1599-1604. [More Information]
Hossain, K., Clayton, D., Goodchild, S., Rodger, A., Payne, R., Cornelius, F., Clarke, R. (2021). Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases. Faraday Discussions, 232, 172-187. [More Information]
Bryant, S., Garcia, A., Clarke, R., Warr, G. (2021). Selective ion transport across a lipid bilayer in a protic ionic liquid. Soft Matter, 17(10), 2688-2694. [More Information]

show 2 more

2020

Yasir, M., Dutta, D., Hossain, K., Chen, R., Ho, K., Kuppusamy, R., Clarke, R., Kumar, N., Wilcox, M. (2020). Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa. Frontiers in Microbiology, 10, 3053. [More Information]
Clarke, R. (2020). One sequence, one structure: Demise of a dogma, or fake news. Journal and Proceedings of the Royal Society of New South Wales, 152(3), 343-345. [More Information]
Gorman, A., Hossain, K., Cornelius, F., Clarke, R. (2020). Penetration of phospholipid membranes by poly-L-lysine depends on cholesterol and phospholipid composition. Biochimica et Biophysica Acta - Biomembranes, 1862 (2), 183128. [More Information]
Chisholm, T., Kulkarni, S., Hossain, K., Cornelius, F., Clarke, R., Payne, R. (2020). Peptide Ligation at High Dilution via Reductive Diselenide-Selenoester Ligation. Journal of the American Chemical Society, 142(2), 1090-1100. [More Information]
Clarke, R., Hossain, K., Cao, K. (2020). Physiological roles of transverse lipid asymmetry of animal membranes. Biochimica et Biophysica Acta - Biomembranes, 1862 (10), 183382. [More Information]
Hossain, K., Li, X., Zhang, T., Paula, S., Cornelius, F., Clarke, R. (2020). Polarity of the ATP binding site of the Na+,K+-ATPase, gastric H+,K+-ATPase and sarcoplasmic reticulum Ca2+-ATPase. Biochimica et Biophysica Acta - Biomembranes, 1862 (2), 183138. [More Information]

show 3 more

2019

Garcia, A., Lev, B., Hossain, K., Gorman, A., Diaz, D., Pham, T., Cornelius, F., Allen, T., Clarke, R. (2019). Cholesterol depletion inhibits Na+,K+-ATPase activity in a near-native membrane environment. Journal of Biological Chemistry, 294(15), 5956-5969. [More Information]
Clarke, R. (2019). Effect of Cholesterol on the Dipole Potential of Lipid Membranes. In Avia Rosenhouse-Dantsker and Anna N. Bukiya (Eds.), Cholesterol Modulation of Protein Function: Sterol Specificity and Indirect Mechanisms, (pp. 135-154). Cham: Springer. [More Information]
Garcia, A., Clarke, R. (2019). Evidence for ATP interaction with phosphatidylcholine bilayers. Biophysical Journal, 116(3 (S1)), 229a. [More Information]
Garcia, A., Pochinda, S., Elgaard-Jørgensen, P., Khandelia, H., Clarke, R. (2019). Evidence for ATP Interaction with Phosphatidylcholine Bilayers. Langmuir, 35(30), 9944-9953. [More Information]
Hossain, K., Clarke, R. (2019). General and specific interactions of the phospholipid bilayer with P-type ATPases. Biophysical Reviews, 11(3), 353-364. [More Information]
Garcia, A., Zou, H., Hossain, K., Xu, Q., Buda, A., Clarke, R. (2019). Polar interactions play an important role in the energetics of the main phase transition of phosphatidylcholine membranes. ACS Omega, 4(1), 518-527. [More Information]

show 3 more

2018

Diaz, D., Clarke, R. (2018). Evolutionary analysis of the lysine-rich N-terminal cytoplasmic domains of the gastric H+,K+-ATPase and the Na+,K+-ATPase. Journal of Membrane Biology, 251(5-6), 653-666. [More Information]
Nguyen, K., Garcia, A., Sani, M., Diaz, D., Dubey, V., Clayton, D., Dal Poggetto, G., Cornelius, F., Payne, R., Separovic, F., Clarke, R., et al (2018). Interaction of N-terminal peptide analogues of the Na+, K+ -ATPase with membranes. Biochimica et Biophysica Acta - Biomembranes, 1860 (6), 1282-1291. [More Information]
Vleeskens, E., Clarke, R. (2018). Kinetic contribution to extracellular Na+/K+ selectivity in the Na+/K+ pump. FEBS Open Bio, 8(5), 854-859. [More Information]

2017

Jiang, Q., Garcia, A., Han, M., Cornelius, F., Apell, H., Khandelia, H., Clarke, R. (2017). Electrostatic stabilization plays a central role in autoinhibitory regulation of the Na+, K+ - ATPase. Biophysical Journal, 112(2), 288-299. [More Information]
Garcia, A., Pratap, P., Lupfert, C., Cornelius, F., Jacquemin, D., Lev, B., Allen, T., Clarke, R. (2017). The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane surface. Biochimica et Biophysica Acta - Biomembranes, 1859 (5), 813-823. [More Information]

2016

Garcia, A., Liu, C., Cornelius, F., Clarke, R., Rasmussen, H. (2016). Glutathionylation-dependence of Na+ -K+ -pump currents can mimic reduced subsarcolemmal Na+ diffusion. Biophysical Journal, 110(5), 1099-1109. [More Information]
Lambropoulos, N., Garcia, A., Clarke, R. (2016). Stimulation of Na+,K+-ATPase Activity as a Possible Driving Force in Cholesterol Evolution. Journal of Membrane Biology, 249(3), 251-259. [More Information]

2015

Garcia, A., Rasmussen, H., Clarke, R. (2015). A novel kinetic model based on the Albers-Post scheme that mimics the behaviour of the Na+, K+-ATPase in situ. Heart, Lung, and Circulation, 24(S3), S224-S224. [More Information]
Clarke, R. (2015). Dipole-potential-mediated effects on ion pump kinetics. Biophysical Journal, 109(8), 1513-1520. [More Information]
Begum, S., Ratna, S., Clarke, R., Ahmed, M. (2015). Excess molar volumes, refractive indices and transport properties of aqueous solutions of poly(ethylene glycol)s at (303.15-323.15) K. Journal of Molecular Liquids, 202, 176-188. [More Information]
Khalid, M., Clarke, R. (2015). Introduction. In Ronald J. Clarke, Mohammed A.A. Khalid (Eds.), Pumps, Channels, and Transporters: Methods of Functional Analysis, (pp. 1-22). Hoboken: John Wiley & Sons. [More Information]
Garcia, A., Eljack, N., Sani, M., Separovic, F., Rasmussen, H., Kopec, W., Khandelia, H., Cornelius, F., Clarke, R. (2015). Membrane accessibility of glutathione. Biochimica et Biophysica Acta - Biomembranes, 1848 (10 (Part A)), 2430-2436. [More Information]
Clarke, R., Khalid, M. (2015). Pumps, Channels, and Transporters: Methods of Functional Analysis. Hoboken: John Wiley & Sons. [More Information]
Clarke, R., Khalid, M. (2015). Stopped-flow fluorimetry using voltage-sensitive fluorescent membrane probes. In Ronald J. Clarke, Mohammed A.A. Khalid (Eds.), Pumps, Channels, and Transporters: Methods of Functional Analysis, (pp. 179-209). Hoboken: John Wiley & Sons. [More Information]

show 4 more

2014

Lev, B., Clarke, R., Allen, T. (2014). Effects of lipid composition on biological membrane electrostatics. Biophysical Journal, 106(2, S1), 80a-80a. [More Information]
Mares, J., Garcia, A., Rasmussen, H., Cornelius, F., Mahmmoud, Y., Berlin, J., Lev, B., Allen, T., Clarke, R. (2014). Identification of Electric-Field-Dependent Steps in the Na+,K+ -Pump Cycle. Biophysical Journal, 107(6), 1352-1363. [More Information]
Eljack, N., Ma, H., Drucker, J., Shen, C., Hambley, T., New, E., Friedrich, T., Clarke, R. (2014). Mechanisms of cell uptake and toxicity of the anticancer drug cisplatin. Metallomics, 6(11), 2126-2133. [More Information]
Khalid, M., Suliman, R., Ahmed, R., Salim, H., Clarke, R. (2014). The high and low affinity binding sites of digitalis glycosides to Na,K-ATPase. Arabian Journal for Science and Engineering, 39(1), 75-85. [More Information]

show 1 more

2013

Garcia, A., Fry, N., Karimi, K., Liu, C., Apell, H., Rasmussen, H., Clarke, R. (2013). Extracellular allosteric Na+ binding to the Na+, K+-ATPase in cardiac myocytes. Biophysical Journal, 105(12), 2695-2705. [More Information]
Clarke, R., Catauro, M., Rasmussen, H., Apell, H. (2013). Quantitative calculation of the role of the Na+ ,K+ -ATPase in thermogenesis. Biochimica et Biophysica Acta, 1827 (10), 1205-1212. [More Information]
Liu, C., Fry, N., Hamilton, E., Chia, K., Garcia, A., Karimigalougahi, K., Figtree, G., Clarke, R., Bundgaard, H., Rasmussen, H. (2013). Redox-dependent regulation of the Na+ -K+ pump: New twists to an old target for treatment of heart failure. Journal of Molecular and Cellular Cardiology, 61, 94-101. [More Information]
Ching, V., Clarke, R., Rendina, L. (2013). Supramolecular beta-cyclodextrin adducts of boron-rich DNA metallointercalators containing dicarba-closo-dodecaborane(12). Inorganic Chemistry, 52(18), 10356-10367. [More Information]
Begum, S., Clarke, R., Ahmed, M., Begum, S., Saleh, M. (2013). Volumetric, viscosimetric and surface properties of aqueous solutions of triethylene glycol, tetraethylene glycol, and tetraethylene glycol dimethyl ether. Journal of Molecular Liquids, 177, 11-18. [More Information]
Clarke, R. (2013). When the going gets hot. Chemistry in Australia, June 2013.

show 3 more

2012

Trevitt, A., Reimers, J., Clarke, R., Vandenberg, J. (2012). BIOPHYSCHEM2011: A joint meeting of the Australian Society for Biophysics and the RACI Chemistry Division. Australian Journal of Chemistry, 65(5), 439-441. [More Information]
Yin, Y., Fisher, K., Nosworthy, N., Bax, D., Clarke, R., McKenzie, D., Bilek, M. (2012). Comparison on protein adsorption properties of diamond-like carbon and nitrogen-containing plasma polymer surfaces. Thin Solid Films, 520(7), 3021-3025. [More Information]
Garcia, A., Rasmussen, H., Apell, H., Clarke, R. (2012). Kinetic comparisons of heart and kidney Na+, K+ -ATPases. Biophysical Journal, 103(4), 677-688. [More Information]
Liu, C., Garcia, A., Mahmmoud, Y., Hamilton, E., Galougahi, K., Fry, N., Figtree, G., Cornelius, F., Clarke, R., Rasmussen, H. (2012). Susceptibility of Î²1 Na+ -K+ pump subunit to glutathionylation and oxidative inhibition depends on conformational state of pump. Journal of Biological Chemistry, 287(15), 12353-12364. [More Information]
Ching, V., Clifford, S., Bhadbhade, M., Clarke, R., Rendina, L. (2012). Synthesis and supramolecular studies of chiral boronated platinum(II) complexes: Insights into the molecular recognition of carboranes by beta-cyclodextrin. Chemistry - A European Journal, 18(45), 14413-14425. [More Information]

show 2 more

2011

Clarke, R. (2011). A perspective on biophysical chemistry. Australian Journal of Chemistry, 64(1), 3-4. [More Information]
Begum, S., Clarke, R., Ahmed, M., Begum, S., Saleh, M. (2011). Densities, Viscosities, and surface Tensions of the System water + Diethylene Glycol. Journal of Chemical and Engineering Data, 56(2), 303-306. [More Information]
Meyers, S., Cornelius, F., Apell, H., Clarke, R. (2011). Kinetics of K+ Occlusion by the Phosphoenzyme of the Na+,K+-ATPase. Biophysical Journal, 100(1), 70-79. [More Information]
Pisani, M., Fromm, P., Mulyana, Y., Clarke, R., Korner, H., Heimann, K., Collins, J., Keene, F. (2011). Mechanism of cytotoxicity and cellular uptake of lipophilic inert dinuclear polypyridylruthenium(II) complexes. ChemMedChem: chemistry enabling drug discovery, 6(5), 848-858. [More Information]
Clarke, R., Fan, X. (2011). Pumping ions. Clinical and Experimental Pharmacology and Physiology, 38(11), 726-733. [More Information]
Clarke, R. (2011). Rapid Reaction Kinetics: Lessons Learnt from Ion Pumps. Australian Journal of Chemistry, 64(1), 5-8. [More Information]
Hosseini, S., Bhadbhade, M., Clarke, R., Rutledge, P., Rendina, L. (2011). Synthesis, carbohydrate- and DNA-binding studies of cationic 2,2':6',2"-terpyridineplatinum(II) complexes containing N- and S-donor boronic acid ligands. Dalton Transactions, 40(2), 506-513. [More Information]

show 4 more

2010

Khalid, M., Cornelius, F., Clarke, R. (2010). Dual Mechanisms of Allosteric Acceleration of the Na+,K+-ATPase by ATP. Biophysical Journal, 98(10), 2290-2298. [More Information]
Clarke, R. (2010). Electric Field Sensitive Dyes. In Alexander P Demchenko (Eds.), Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design, (pp. 331-344). Berlin Heidelberg: Springer. [More Information]
Amoroso, S., Clarke, R., Larkum, A., Quinnell, R. (2010). Electrogenic plasma membrane H+-ATPase activity using voltage sensitive dyes. Journal of Bioenergetics and Biomembranes, 42(5), 387-393. [More Information]
Khalid, M., Fouassier, G., Apell, H., Cornelius, F., Clarke, R. (2010). Interaction of ATP with the phosphoenzyme of the Na+,K +-ATPase. Biochemistry, 49(6), 1248-1258. [More Information]
Noske, R., Cornelius, F., Clarke, R. (2010). Investigation of the enzymatic activity of the Na+,K+-ATPase via isothermal titration microcalorimetry. Biochimica et Biophysica Acta, 1797 (8), 1540-1545. [More Information]
Ash, M., Guilfoyle, A., Clarke, R., Guss, M., Maher, M., Jormakka, M. (2010). Potassium-activated GTPase reaction in the G protein-coupled ferrous iron transporter B. Journal of Biological Chemistry, 285(19), 14594-14602. [More Information]

show 3 more

2009

Starke-Peterkovic, T., Clarke, R. (2009). Effect of headgroup on the dipole potential of phospholipid vesicles. European Biophysics Journal, 39(1), 103-110. [More Information]
Clarke, R. (2009). Mechanism of allosteric effects of ATP on the kinetics of P-type ATPases. European Biophysics Journal, 39(1), 3-17. [More Information]
Pilotelle-Bunner, A., Cornelius, F., Sebban, P., Kuchel, P., Clarke, R. (2009). Mechanism of Mg(2+) binding in the Na+,K+ -ATPase. Biophysical Journal, 96(9), 3753-3761. [More Information]
Clarke, R. (2009). Probing Kinetics of Ion Pumps via Voltage-Sensitive Fluorescent Dyes. In Ewa M Goldys (Eds.), Fluorescence Applications in Biotechnology and the Life Sciences, (pp. 349-363). New Jersey, USA: John Wiley & Sons.
Guilfoyle, A., Maher, M., Rapp, M., Clarke, R., Harrop, S., Jormakka, M. (2009). Structural basis of GDP release and gating in G protein coupled Fe(2+) transport. EMBO Journal, 28(17), 2677-2685. [More Information]
Pilotelle-Bunner, A., Beaunier, P., Tandori, J., Maroti, P., Clarke, R., Sebban, P. (2009). The local electric field within phospholipid membranes modulates the charge transfer reactions in reaction centres. Biochimica et Biophysica Acta, 1787 (8), 1039-1049. [More Information]

show 3 more

2008

Pilotelle-Bunner, A., Matthews, J., Cornelius, F., Apell, H., Sebban, P., Clarke, R. (2008). ATP Binding Equilibria of the Na(+),K(+)-ATPase. Biochemistry, 47(49), 13103-13114. [More Information]
Pham, T., Clarke, R. (2008). Solvent Dependence of the Photochemistry of the Styrylpyridinium Dye RH421. The Journal of Physical Chemistry B, 112(20), 6513-6520. [More Information]

2007

Clarke, R., Apell, H., Kong, B. (2007). Allosteric effect of ATP on Na+,K+-ATPase conformational kinetics. Biochemistry, 46(23), 7034-7044. [More Information]
Hansen, P., Clarke, R., Buhagiar, K., Hamilton, E., Garcia, A., White, C., Rasmussen, H. (2007). Alloxan-induced diabetes reduces sarcolemmal Na+-K+ pump function in rabbit ventricular myocytes. American Journal of Physiology: Cell Physiology, 292(0), C1070-C1077. [More Information]
Vitha, M., Clarke, R. (2007). Comparison of excitation and emission ratiometric fluorescence methods for quantifying the membrane dipole potential. Biochimica et Biophysica Acta - Biomembranes, 1768 (0), 107-114. [More Information]
Le Goff, G., Vitha, M., Clarke, R. (2007). Orientational polarisability of lipid membrane surfaces. Biochimica et Biophysica Acta, 1768 (0), 562-570. [More Information]
Clarke, R., Kane, D. (2007). Two gears of pumping by the sodium pump. Biophysical Journal, 93(12), 4187-4196. [More Information]

show 2 more

2006

Starke-Peterkovic, T., Turner, N., Vitha, M., Waller, M., Hibbs, D., Clarke, R. (2006). Cholesterol effect on the dipole potential of lipid membranes. Biophysical Journal, 90(11), 4060-4070. [More Information]
Amoroso, S., Agon, V., Starke-Peterkovic, T., McLeod, M., Apell, H., Sebban, P., Clarke, R. (2006). Photochemical behavior and Na+,K+-ATPase sensitivity of voltage-sensitive styrylpyridinium fluorescent membrane probes. Photochemistry and Photobiology, 82(2), 495-502. [More Information]

2005

Starke-Peterkovic, T., Clarke, R., Turner, N., Else, P. (2005). Electric field strength of membrane lipids from vertebrate species: membrane lipid composition and Na+-K+-ATPase molecular activity. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 288(3), R663-R670. [More Information]
William, M., Vien, J., Hamilton, E., Garcia, A., Bundgaard, H., Clarke, R., Rasmussen, H. (2005). The nitric oxide donor sodium nitroprusside stimulates the Na+-K+ pump in isolated rabbit cardiac myocytes. Journal of Physiology, 565.3(3), 815-825. [More Information]

2004

Buhagiar, K., Hansen, P., Kong, B., Clarke, R., Fernandes, C., Rasmussen, H. (2004). Dietary Cholesterol Alters Na+/K+ Selectivity At Intracellular Na+/K+ Pump Sites In Cardiac Myocytes. American Journal of Physiology: Cell Physiology, 286(2), C398-C405.
Clarke, R., Oprysa, A. (2004). Fluorescence And Light Scattering. Journal of Chemical Education, 81(5), 705-707.
Kong, B., Clarke, R. (2004). Identification Of Potential Regulatory Sites Of The Na+,K+-Atpase By Kinetic Analysis. Biochemistry, 43(8), 2241-2250. [More Information]

2003

Nieuwenburg, P., Clarke, R., Cai, Z., Chen, M., Larkum, A., Cabral, N., Ghiggino, K., Reimers, J. (2003). Examination of the photophysical processes of chlorophyll d leading to a clarificaiton of proposed uphill energy transfer processess in cells of Acaryochloris marina. Photochemistry and Photobiology, 77(6), 628-637. [More Information]
Wang, Y., Winnik, F., Clarke, R. (2003). Interaction between DMPC liposomes and HM-PNIPAM polymer. Biophysical Chemistry, 104(2), 449-458. [More Information]
Clarke, R., Humphrey, P., Lupfert, C., Apell, H., Cornelius, B. (2003). Kinetic investigations of the mechanism of the rate-determining step of the Na+, K+-ATPase pump cycle. Annals of the New York Academy of Sciences, 986, 159-162. [More Information]

2002

Hansen, P., Buhagiar, K., Kong, B., Clarke, R., Gray, D., Rasmussen, H. (2002). Dependence of Na+-K+ pump current-voltage relationship on intracellular Na+, K+, and Cs+ in rabbit cardiac myocytes. American Journal of Physiology: Cell Physiology, 283(5), 1511-1521. [More Information]
Schamberger, J., Clarke, R. (2002). Hydrophobic Ion Hydration and the Magnitude of the Dipole Potential. Biophysical Journal, 82(June 2002), 3081-3088. [More Information]
Humphrey, P., Lupfert, C., Apell, H., Cornelius, F., Clarke, R. (2002). Mechanism of the Rate-Determining Step of the Na+,K+-ATPase Pump Cycle. Biochemistry, 41(30), 9496-9507. [More Information]

2001

Clarke, R., Bamberg, E., Fendler, K. (2001). Electrogenic properties of the Na+, K+ -ATPase probed by presteady state and relaxation studies. Journal of Bioenergetics and Biomembranes, 33, 401-405.
Clarke, R., Frank, J., Vater, J., Holzwarth, J. (2001). Influence of allosteric effectors on the kinetics and equilibrium binding of phosphoenolpyruvate (PEP) to phosphoenolpyruvate carboxylase (PEPC) from Zea mays. Biophysical Chemistry, 92, 53-64. [More Information]
Lupfert, C., Grell, E., Pintschovius, V., Apell, H., Cornelius, B., Clarke, R. (2001). Rate limitation of the Na+, K+ -ATPase pump cycle. Biophysical Journal, 81(4), 2069-2081.
Clarke, R. (2001). The dipole potential of phospholipid membranes and methods for its detection. Advances in Colloid and Interface Science, 89-90, 263-281.

show 1 more

Selected Grants

2021

Membrane Interaction of Small Molecules Capable of Improving Battens Disease Cell Phenotypes, Clarke R, Hossain K, Batten Disease Support and Research Association, Australian Chapter/Research Grant

2016

How cholesterol optimises ion pump function in animal membranes, Clarke R, Allen T, Cornelius F, Australian Research Council (ARC)/Discovery Projects (DP)

2014

Lipid-protein interplay in the mechanism of the sodium pump, Clarke R, Cornelius F, Australian Research Council (ARC)/Discovery Projects (DP)

2013

University of Sydney Automated Peptide Synthesis Platform, Payne R, Britton W, Byrne S, Chan H, Clarke R, Hunt N, Jolliffe K, Kassiou M, Lay P, Mackay J, Matthews J, McErlean C, New E, Rasmussen H, Rendina L, Rudrawar S, Rutledge P, Shepherd N, National Health and Medical Research Council (NHMRC)/Equipment Grant

2012

SF-61SX2/s stopped-flow fluorimeter and Service and upgrade of SX-17MV stopped-flow spectrometer, Clarke R, Davies M, Lay P, Witting P, Rasmussen H, Matthews J, Pattison D, Vandenberg J, National Health and Medical Research Council (NHMRC)/Equipment Grant

2011

Electromechanical controls of membrane transport phenomena, Allen T, Clarke R, Australian Research Council (ARC)/Discovery Projects (DP)

2010

Automated storage, retrieval, and UV imaging system, Jormakka M, Gamble J, Semsarian C, Rasko J, Vadas M, Xia P, Gorrell M, Clarke R, Holst J, Church W, National Health and Medical Research Council (NHMRC)/Equipment Grant

2005

Regulation of the sarcolemmal Na-K pump by FXYD proteins associated with it, Rasmussen H, Marassi F, Clarke R, National Health and Medical Research Council (NHMRC)/Project Grants

2004

In situ measurements of the electorstatic potential within redox proteins via a quinone-linked voltage-sensative fluorescent probe, Clarke R, Goldys E, Kable S, ARC/NHMRC Research Network for Fluorescence Applications in Biotechnology and Life Sciences (NHMRC)/FABLS Support Scheme for Emerging Research Projects

2003

In situ measurements of the electrostatic properties inside photosynthetic reaction centres, Clarke R, Australian Research Council (ARC)/Linkage International: ARC International Fellowships (ARCIF)

2001

Function and regulation of the Na+,K+-ATPase, Apell H, Clarke R, Cornelius B, Else P, Australian Research Council (ARC)/Discovery Projects (DP)

2000

Mechanism of the rate-determining step of the Na+, K+-ATPhase, Clarke R, DVC Research/Research and Development Scheme: Newly Appointed Staff (NAS)

1999

Mechanism of local anaesthetics, Clarke R, University of Sydney/University Research Grant

Faculty of Science

Page not found

Associate Professor Ronald Clarke

Selected publications

Publications

By Type

By Year

Selected Grants

2021

2016

2014

2013

2012

2011

2010

2005

2004

2003

2001

2000

1999