Transmission electron microscopy

This new high-resolution TEM has been installed at our Sydney Nano Hub lab.

System specifications:

• 60-300 kV
• 3 condensor lenses
• X-FEG high brightness gun
• Monochromator
• SuperX-EDS (quad) detector system
• Ceta CMOS camera
• S-TWIN (+/-40° α, +/-30° β)
• 4k x 4k STEM imaging
• TEM information limit @ 300 kV: ≤ 1Å
• STEM point resolution @ 300 kV: ≤ 1.36Å

Specialist: Dr Magnus Garbrecht (magnus.garbrecht@sydney.edu.au)

This microscope which is located at the Sydney Nanoscience Hub allows for combined high spatial and spectral analysis of materials via atomically resolved imaging and elemental mapping with energy-dispersive X-ray and electron-energy loss spectroscopies at a range of voltages.

• High stability environmental enclosure
• X-FEG high-brightness gun
• Monochromator
• Probe DCOR+ Corrector (point resolution <0.6Å)
• Image DCOR+ Corrector (information limit <0.6Å)
• ChemiSTEM (Super-X) EDS detectors
• Gatan Quantum ER/965 GIF (<0.14 eV (1s))
• Dual EELS + Ultrafast shutter system (1000spectra/s)
• Ceta 16M camera
• Lorentz lens
• 60,120,200,300 kV
• Single- and low-background double-tilt-, Tomography-, and combined in-situheating/biasing holders

Specialist: Dr Magnus Garbrecht (magnus.garbrecht@sydney.edu.au)

The Thermo Glacios is a 200kV dedicated cryo-electron microscope. Fitted with a field emission gun and a Falcon3 direct electron detector it can produce micrographs suitable for high-resolution single particle analysis. Eleven grids can be loaded into the instrument at a time via the autoloader, allowing rapid grid screening.
System specifications:
- 200kV FEG source
- Falcon3 DED + Ceta 16M CMOS camera
- Autoloader system
- Single-axis stage tilting for tomography capability
- EPU software for single particle analysis
- SerialEM for tomography  

Specialists (Bio): 
Dr Errin Johnson  (errin johnson@sydney.edu.au)
Bhanu Mantri (bhanu.mantri@sydney.edu.au)

The JEOL 2200FS is one of the latest 200 kV field emission gun TEMs from JEOL. It provides a high coherence, high-current beam with a narrow energy spread and a current of 0.5 nA in a 1 nm probe. An advanced EDS and STEM system permits point, line scan and area mapping with X-rays and various electron signals. The built-in Omega filter enables energy-filtered imaging and energy loss spectroscopy.

• Point resolution of 0.23 nm
• Lattice resolution of 0.1 nm
• Omega filter for EELS/EFTEM
• EELS resolution of 0.8 eV
• Gatan Ultrascan 2 k x 2 k camera
• STEM using BF (energy-filtered), HAADF and X-ray signals
• Tomography and in-situ heating capabilities built-in
• Hollow cone dark field imaging

Specialist: Dr Hongwei Liu (hongwei.liu@sydney.edu.au)

This 200 kV instrument is configured with a cryo-polepiece, specifically designed for cryo-microscopy and cryo-tomography TEM to high tilts. It uses a high brightness LaB₆ electron source. A range of specialized cryo- and tomography holders enable 3-D imaging (tomography) of plunge frozen sections. Gatan large-area and high-resolution cameras are fitted. The instrument is also a useful platform for room temperature 2-D/3-D imaging of thick biological sections and materials specimens.

• Point resolution of 0.27 nm
• Lattice resolution of 0.14 nm
• Gatan Erlangshen (1.35 k x 1.08 k) and Ultrascan (2 k x 2 k) cameras
• Tomography, in-situ heating, deformation capabilities built-in
• Tilt angles up to ±80°
• Gatan cryo, cryo-tomography and double-tilt tomography holders

Specialists: 

Dr Hongwei Liu (hongwei.liu@sydney.edu.au)
Dr Takanori Sato (takanori.sato@sydney.edu.au)

This 120 kV TEM is a general purpose TEM for routine biological and materials characterisation. It has a high brightness LaB₆ electron source and an easy-to-use interface. A Gatan large-area camera is fitted, enabling effortless imaging. A STEM unit allows bright- and dark-field imaging. A JEOL EDS system makes this an ideal platform for users wishing to carry out routine X-ray microanalysis and elemental profiling and mapping.

• Point resolution of 0.38 nm
• Lattice resolution of 0.2 nm
• Gatan Erlangshen (1.35 k x 1.08 k) camera
• STEM imaging with BF, DF and X-ray signals
• JEOL EDS system

Specialists: 
Dr Hongwei Liu (hongwei.liu@sydney.edu.au)
Dr Errin Johnson  (errin johnson@sydney.edu.au) (Bio)

The FEI T12 is a general purpose life science TEM operating at 120 kV. It is useful for screening of beam sensitive samples as well as low to medium range magnification imaging.

Specialists:

Dr Hongwei Liu (hongwei.liu@sydney.edu.au)
Dr Takanori Sato (takanori.sato@sydney.edu.au)