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Veterinary Pathology Diagnostic Services

Providing reliable and high quality diagnostics
We provide high quality services to the University, veterinary profession & research organisations. Our pathologists and highly skilled technical staff ensure a reliable and high quality service to our clients.

Services

We provide high quality biopsy and necropsy services to the veterinary profession, servicing the University Veterinary Hospitals and large and small private practices. We offer a service that entails a strong review process, with all specimens being reviewed by multiple pathologists, and a personalised interaction with the veterinarian. 

Our pathologists are readily available for comment on individual cases and can help veterinarians bring their clinical research to publication standard. In addition, we offer a high quality reliable service to researchers in histopathological specimen processing, immunohistochemistry, and processing of unusual specimens. Our highly skilled technical staff are available for consultation about unusual or difficult specimens.

Service to researchers

  • histopathological specimen processing 

  • immunohistochemistry

  • laboratory animal haematology

  • serum biochemistry

  • processing of unusual specimens

Sending tissue through the post:

  • Place tissue in 10% neutral buffered formalin (volume tissue to formalin 1:20) 
  • Tissue should preferably be fixed for 24 hours at a minimum. 
  • Remove most to all of the fixative. In a zip lock plastic bag, seal the tissue with moistened gauze(s). Double seal or triple seal the bag with the specimen, with another zip lock bag(s) to minimise leakage and then place into IATA compliant packaging.
  • DO NOT LET SAMPLES DRY OUT during transport. Label bag with specimen details. 
  • Send information sheet with sample details separately to specimen and in another bag to avoid leakage onto writing. 
  • Instead of zip lock bags, wide neck containers, leak proof and screw top, can be used instead. Minimize formalin and send with moistened gauze. Minimize the use of tape before putting the lid on as it acts as a conduit for formalin to leak out. Label container with specimen details and double seal the containers with zip lock bags. Send information sheet with patient details separately. 
  • If excess liquid /formalin is sent through the mail, it must be labelled as 10% NB Formalin under legislation with an MSDS. NO concentrated formalin (>10%) should be sent via mail.

The Clinical Pathology Lab is equipped to carry out diagnostic haematology, biochemistry, microbiology and cytology. The lab predominantly services the UVTH-Sydney, running routine diagnostic tests on companion animals. 
 
Clinical Pathology is also in a position to offer research groups, from the University and external institutions, biochemistry and haematology on its state of the art analysers. This service is available for lab animals and wildlife on request. 
 
For enquiries regarding specimen collection contact the Lab Manager.

The Molecular Diagnostics section performs pathogen detection using a wide range of sensitive molecular diagnostic methods, including highly sensitive and specific conventional and real-time PCRs; immunohistochemistry and in-situ hybridization for fresh and formalin-fixed tissues; immunofluoresence for detection of pathogens in body fluids, cytological preparations and fecal parasites; and 16s PCR and sequencing for identification of bacteria from cultures.  
 
Test development draws on the diverse expertise of our pathobiology research group, who not only have expertise in the laboratory setting, but have backgrounds in management of the species involved. This ensures results are interpreted by leaders in the field, with a deep understanding of the test, the pathogen it is detecting, and its impact on animal management.  
 
Our greatest expertise lies in detection of pathogens associated with companion animal and wildlife species, and we welcome the opportunity to discuss development of new assays for support of animal management or research in these specialist areas. 
 
Please find Fecal Panel launch (pdf, 6MB)

Available tests

Test Category Test Name
Immunofluorescence FCoV (FIPV) (cytospin/effusion)
FeLV (EDTA blood) FIV (EDTA blood)
Immunohistochemistry (FFPE tissue) Chlamydia
FCoV (FIPV)
Feline Calicivirus
Feline Herpes Virus Toxoplasma*
Real-time PCR (swab/tissue) Chlamydia pecorum
Chlamydia pneumoniae
Chlamydia genus
PCR and sequencing* Bacterial ID 16s Fungal ID 18s
Serology (serum) Cryptococcus Ag LCAT
Cryptococcus Ag lateral flow ELISA
 
Faecal protozoal detection* Cryptosporidium, Giardia, T. fetus 
(culture and immunofluorescence)
T. fetus (culture)
Associated Profiles Koala Chlamydia PCR 
(ocular and urogenital swabs, C. pecorum and
C.pneumoniae)
Koala screening 
(Koala Chlamydial PCR, Cryptococcus Ag ELISA)
Koala Health Profile (FBC and biochemistry) 

Note: For tests marked *, contact lab before submission 

Section Head: Dr Damien Higgins

The Diagnostic Parasitology section is responsible for the detection and identification of external and internal parasites from a diverse animal hosts, these include pet, pocket, exotic as well domestic and wild animal species. We receive variety of materials and specimens, including faecal samples, aspirates, skin scrapings, sticky tapes as well as presumed parasites for identification. We use traditional processing methods as well as immunological assays for increased sensitivity and specificity. 
 
The laboratory’s responsibilities are processing and analysing of the received samples, and reporting results based on routine methods. On demand, the laboratory develops new methods or we adopt existing methods based on the client requirements, under these circumstances we will conduct quality control to assure quality results.

Section head: Professor Jan Slapeta   

Routine tests:

  • Direct Smear (all animal species, a fresh specimen required)

  • Faecal Flotation (NaCl solution, other solutions on demand; all animal species) 

  • McMaster Faecal Egg Counts (all species)

  • Baermann test for lungworms (all animal species)

  • Sedimentation techniques (qualitative & quantitative; all animal species)

  • Direct Fluorescence Test for Cryptosporidium and Giradia 
    - all mammalian species; only experimental for birds, reptiles and fish

  • Tritrichomonas foetus Culture 
    - cats; only experimental for dogs and other potential hosts

On demand tests 
On demand parasitological examinations and development usually include molecular methods such as PCR and immunodiagnostic tests.  
 
The laboratory is equipped to adopt and develop published methods or design new based on the needs of the clients. Using these activities the laboratory participates in field investigations leading to better understanding of parasite epidemiology.

Turnaround time of routine test is usually 24 hours of sample receipt. Turnaround time for ‘on demand’ diagnostics varies and is usually discussed with the client to meet their needs and capability of the laboratory.

  • A/Prof Damien Higgins ,Director, Veterinary Pathologist
  • Dr Derek Spielman, Deputy Director of VPDS (Veterinary Pathologist)
  • George Tsoukalas, Technical Manager (Lab Manager) VPDS

  • Dr Shannon Donahoe, Veterinary Anatomical Pathologist
  • Prof Jan Slapeta, Veterinary Parasitologist
  • A/Prof Jacqui Norris, Veterinary Microbiologist 
  • Dr Rachael Gray, Veterinary Clinical Pathologist
  • A/Prof Mark Krockenberger, Specialist Veterinary Anatomical Pathologist
  • Patricia Martin, Diagnostic Microbiologist/Cytologist 
  • Denise McDonell, Laboratory Diagnostician
  • Karen Barnes, Histopathology (Technician)
  • Andrew Fortis, Histopathology (Technician)
  • Christine Gotsis, Clinical Pathology (Technician)
  • Elaine Chew, Histopathology (Technician)
  • Maira Meggiolaro, Molecular Diagnostics (Technician)

Laboratory Fees (ex GST) 2018 

  

Haematology 

$

70045 

Full Blood Count (Retics when required) 

51.5 

70147 

FBC (Manual Validation) 

31 

70046 

PCV & TPP billed as one test 

20.5 

90043 

FBC - no diff (Sysmex) 

17 

90044 

FBC + diff (Sysmex) 

20 

90045 

FBC + diff + retic (Sysmex) 

23 

70124 

The FIRST test 

21 

70125 

EACH Additional Test 

12.5 

  

Except for:

  

70044 

Fibrinogen - heat 

19.5 

70049 

Reticulocyte Count 

21.5 

70041 

Bone marrow - Quantitative assessment 

123.5 

70048 

Bone Marrow - quantitative assessment plus histology 

134 

70056 

Cross Match Just one donor 

47 

70057 

Cross Match Just two donor 

64 

70052 

Each additional donor 

21.5 

  

Biochemistry 

  

70002 

First Test 

21 

70004 

Each Additional Test 

10.5 

  

Except for:

  

70005 

Ammonia - 1 Sample 

38 

70006 

Ammonia Tolerance - 2 Samples 

53.5 

70007 

Amylase 

12.5 

70011 

Electrolytes Alone 

22.5 

70012 

Electrolytes - Na, K+, Cl- 

17.5 

70014 

Lipase 

22.5 

70024 

Triglycerides 

13.5 

70120 

AST 

12.5 

70121 

GGTP 

12.5 

70152 

CRP 

38 

70163 

Canine PL 

50 

70164 

Feline PL 

50 

70148 

Total T4 

40 

70149 

Total T4 (add. test) 

19.5 

70158 

Canine TLI 

72 

70159 

Canine Total T4 

40 

70160 

Canine TSH 

51 

70161 

Vet Free T4 

51 

70162 

Thyroid- Free T4 & TSH (combo) 

92 

90017 

Globulin (panel) 

32 

70016 

Profile A Non Specific 

76 

70017 

Profile B Alimentary 

40 

70021 

Profile F Pre Anaesthesia 

40 

70138 

Profile Renal 

37.5 

70144 

Profile Liver 

53.5 

70153 

Profile Cardiac 

68 

90147 

Profile K 

54.5 

  

Urine 

  

70112 

Chemical & Microscopic 

39 

70113 

Chemical only 

17 

70114 

Microscopic only 

31 

  

Combinations 

  

70661 

Feline Combination - (FBC/Profile A no Amy or LIP) 

102 

70662 

Feline Combination Plus - FBC/Profile A no Amy or LIP + Urinalysis 

127.5 

70663 

Canine Combination - (FBC/Profile A + Amy + LIP) 

109.5 

70664 

Canine Combo Plus- (FBC/Profile A + Amy + LIP + Urinalysis) 

134 

70146 

Health Profile 

81 

70665 

Urine C/S + Urinalysis 

94 

70666 

UPC/Urinalysis 

63.5 

70129 

Urine Protein/Creatinine Ratio 

32.5 

90146 

Koala Health Profile 

75 

  

Histopathology 

  

70059 

Biopsy report - first specimen/site 

113.5 

70134 

Biopsy report - additional specimen/site 

21.5 

70058 

Histology Preparation - one specimen/site 

28.5 

90060 

PM - Histo only (externally performed PM) 1-6 tissues 

198 

70656 

PM histology - additional tissue samples 

18 

90059 

Biopsy - no report 

52.5 

70135 

Second Opinion - Slide Preparation 

54 

90135 

Biopsy Report - Second Opinion 

53.5 

70667 

Special stains - non-silver 

28.5 

70668 

Special stains - silver 

28.5 

90660 

PCR slide cutting 

28.5 

90661 

Oversize slide 

28.5 

90662 

SLIDE SCAN- HISTO 

28 

  

Immunohistology 

  

70136 

IHC - FIP confirmation test 

127.5 

70145 

IHC - CD3/CD79a panel 

165 

70401 

IHC - First Antibody 

127.5 

70657 

IHC - Additional Slides/Antibody 

28.5 

70402 

IHC - Additional Antibody 

63.5 

  

Post-mortems 

  

70100 

PM - Gross/Histo - Birds 

120.5 

70101 

PM - Gross/histo - Lab Animals 

161.5 

70102 

PM - Gross/Histo - Small animals (< 10kg) 

478 

70103 

PM - Gross/Histo - Large Animals (>10kg) 

584 

70106 

PM - Gross/Histo Additional Spinal Cord 

212 

  

Microbiology 

  

70070 

Culture & Sensitivity 

77 

70069 

Culture & Sensitivity (tissue) 

85 

90067 

C & S 2 Similar sites Tissue (external) 

112.5 

70067 

Culture & Sensitivity of two similar sites 

107 

70083 

Urine Culture & Sensitivity 

77 

70068 

Blood Culture & sensitivity in Oxoid 1st with urine 

124 

70080 

Oxoid Blood Culture  - Each subsequent Oxoid sample 

21 

70157 

Oxoid Blood C/S- without Urine 

100 

70060 

BactT Alert C&S Blood - without Urine 

75 

70061 

BacT Alert C&S Blood & Urine - Client Media 

102 

70075 

Fungal Culture - Non-dermatophyte 

70.5 

70076 

Fungal culture - Dermatophyte 

57.5 

70501 

Mycobacteria or Nocardia culture & sensitivity 

127.5 

70077 

LCAT (Cryptococcal  Serology) 

92.5 

70150 

Lateral Flow (Cryptococcal Serology) 

40 

70132 

Fungal identification 

87.5 

70133 

Fungal sensitivity 

101.5 

70658 

Postage & Handling (/$0.01) 

25 

90076 

Gram Stain 

36.5 

  

Cytology 

  

70033 

FNA/Smear - First Site 

68 

70031 

FNA/Smear - Second Site 

39 

70034 

FNA/Smear - Third + Subsequent Sites 

21 

70659 

FNA/Smear - More Than 10 Slides From Same Site 

35 

70032 

Fluid Analysis: cytology, TP, all fluids 

81 

70655 

CSF Analysis 

95.5 

70140 

IF - FIP + others 

92.5 

  

Parasitology 

  

70090 

Nematodes, Coccidia, (Float or eggs/gram) 

37.5 

70654 

DFA (Giardia/Crypto) 

57.50 

70653 

Tritrichomonas PCR 

63.00 

70091 

Lungworm (Baermann) 

47 

70092 

Faecal Culture & identification 

70 

70095 

Identification from 

41 

70096 

Skin- Digest 

55.5 

70097 

Viscera-Total worm count or Digest. 

86 

  

Molecular diagnostics 

  

92013 

qPCR 1-2 Targets 

63 

92014 

qPCR Add. target 

40 

92015 

qPCR 3-7 targets 

75 

92016 

qPCR > 7 targets 

92.5 

92009 

Avian Panel qPCR 

75 

92003 

Bacterial ID - PCR 

112.5 

92004 

Fungal ID - PCR 

112.5 

92005 

Mycobacterial ID - PCR 

112.5 

92006 

DNA Extraction (/Sample) 

23 

92012 

Sequencing 

20 

 

George Tsoukalas

Laboratory Manager
Address
  • Veterinary Pathology Diagnostic Services Sydney School of Veterinary Science B14 - McMaster Building, Room 213-220 The University of Sydney NSW 2006 Australia