People_
Associate Professor Jim Manos
BSc(Hons 1st Class), PhD, FASM
Professor in Infectious Diseases
Medicine (Immunology and Infectious Diseases)
School of Medical Sciences
Medicine (Immunology and Infectious Diseases)
School of Medical Sciences
Details
Member of the Charles Perkins Centre
Associate Professor Jim Manos has a growing international reputation in the field of bacterial pathogenesis with a focus on bacterial biofilm infections ((46 res. articles, 4 book chapters: 2500 lifetime citations, 1200 since 2019 h-index =27 (Google Scholar 25-9-24)). He has recently written an invited review article: Microbiomes in Pathology and Disease (APMIS 2022: 93 citations). A second review article on Cystic Fibrosis pathogens was published in 2021 (Microorganisms) and formed the basis of his award of Fellow of the Australian Society for Microbiology (ASM) in 2021. Since 2005 he has been a CI on successful grants totalling $5,200,000. For three of these grants, totalling $4,285,000, including the current iMCRC grant with Whiteley Corporation (IMCRC/WLY/08052019.1) he has been the program leader. He completed his PhD at UNSW (1999) and spent five years at the University of Maryland School of Medicine (Baltimore, USA) studying the flagellin switching mechanism of Proteus mirabilis (five publications 2001-2004) and has been at the University of Sydney since 2004 initially researching Cystic fibrosis infections before branching out into the study of bacterial biofilms. He is currently Associate Professor in Infectious Diseases in the School of Medical Sciences, where he established his research team as Head of the Bacterial Pathogens Research Group.
- Associate Professor Manos is the recipient of an Innovative Manufacturing CRC grant ($4.152 million 2018-2022) which has generated eight publications. Since 2023 he has also received funding from Whiteley Corporation and Sydney Infectious Diseases Institute ($380,000; 2023-2025) to employ Post Doctoral Fellow Dr Arthika Manoharan to continue the UTI research work and sucessfully obtained a Seed funding grant from the same organisations ($10,000) to expand the Bacterial Vaginosis research. He was previously the recipient of a Commercial Development & Industry Partnership (CDIP) Seed grant with CIB Whiteley ($84K 2017-18) which generated their joint first publication in the CT field (#JM3). CIA Manos was CIB on NHMRC#632788 ($427,000 2010-2013) which generated eight publications. He was the recipient of an Early Career Researcher Grant (ECR2009) Faculty of Medicine, University of Sydney ($49,450) and has also been a CI on five Australian Cystic Fibrosis Trust (ACFT) grants and one University of Sydney Sesqui Grant, with a total value of $425,000. He is also part of two other recent University of Sydney Seed grants (2018 and 2022). Invited and Oral Presentations. In the past year, A/Prof Manos has been an invited symposium speaker at the Australian Society for Microbiology Annual Conference (Sydney Convention Centre July 11-14, 2022) and the Asia-Pacific Biofilms conference (virtual- October 18-23 2022). He has given many other presentations, beginning in 1993, the most recent at the State University of New York (SUNY) in Buffalo NY USA on January 10 2023. Since 2017, Associate Professor Jim Manos has: • Established that the antioxidant glutathione, the precursor of N N-acetylcysteine effectively disrupts bacterial biofilms and enables antibiotics to eradicate the exposed bacteria AAC 60 (8) 2016. 56 cit.]. The outcomes of this publication and preliminary data on CT effects on other bacteria led CIA Manos to partner with CIB Whiteley from Whiteley Corporation and apply for two patents for CT: • Biofilm-disrupting composition for use on chronic wounds TDA Kumar, J Manos, GS Whiteley, TO Glasbey- US Patent App. 16/470,904, 2020. • Biofilm-disrupting composition TDA Kumar, J Manos, GS Whiteley, TO Glasbey- US Patent App. 16/470,887, 2020. Process of treating urinary tract infections (AU24-10-2022-903139) Manoharan A, Manos J, Whiteley G.S., Glasbey T.O.The first of these was granted (October 2022), the second is in the PCT examination stage while the third is at the pre-examination stage. • Established the efficacy and cytotoxicity of GSH [Front. Microbiol. 8, 2429 2017 23 cit.]. • Demonstrated the efficacy of the stable GSH derivative N-acetylcysteine (NAC), in a range of clinically derived biofilm-forming pathogens from various host infection sites [Front. Microbiol. 10 2000 14 2019. 24 cit.; J. Antimicrob. Chemother. 75 (7), 1787-1798 2020 10 cit.; four 2021 publications (Aiyer et al., Manoharan et al.,) 30 cit. and three 2022 publications (7 cit.)]
Recently in the media:
https://www.medicalrepublic.com.au/__azithromycin_cysticfibrosis/71911
Research Training and Supervision As primary supervisor, Associate Professor Manos has supervised four PhD completions, ten Honours students (8 Class 1) and one M(Phil). He is currently primary supervisor of one Honours student. Undergraduate and Postgraduate Teaching CIA Manos is currently teaching in three courses: one undergraduate (INFD3888–Infectious Diseases: 2024: 28 students) and three postgraduate courses: the Graduate Medical and Dental Programs (GMP/GDP: 2024: 360 students) and the Graduate Program in Infection and Immunity (INIM) (2024: 18 students). He ran the Infection and Immunity Graduate program (INIM) for the School of Medical Sciences as the Course Coordinator 2009-21. In his career he has mentored and assisted over 245 students.
Treatment therapies for Biofilm infections (see grants)
Scientific Associations: CIA Manos was inducted as a Fellow of The Australian Society for Microbiology (FASM) in 2021. He is a committee member (since 2010) and was Treasurer of the ASM NSW Branch 2012-18 and Branch President 2019-2021. He is an active participant in ASM meetings. He is a member of the American Society for Microbiology since 2000.
2021 Fellow of the Australian Society for Microbiology (FASM). 2013 Overseas travel grant by (FEMS): to attend ‘Pseudomonas2013’ in Lausanne Switzerland. 1997 Young Scientist Award, by The European Helicobacter pylori Study Group (EHPSG). 1996 Postgraduate Travel Award, by: School of Microbiology and Immunology University of New South Wales.
Infection and Immunological Conditions, Lifespan
(IBIS, Laval University Quebec City, Quebec Canada) Collaborated with Prof. Roger Levesque and his team at the Institut de Biologie Integrative et des Systemes (IBIS, to sequence and phylogenetically compare the Australian Epidemic Strain -1 (AES-1) to other epidemic and non-epidemic P. aeruginosa strains worldwide. | |
(Prof Craig Winstanley University of Liverpool) Collaborated to put together Pseudomonas aeruginosa strains to form the International reference panel of CF-associated P. aeruginosa strains. |
Publications
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Book Chapters
- Ashish Kumar, T., Manoharan, A., Whiteley, G., Glasbey, T., Manos, J. (2020). Pseudomonas aeruginosa biofilms and infections: Roles of extracellular molecules. In Mukesh Kumar Yadav and Bhim Pratap Singh (Eds.), New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms: Current Research and Future Trends, (pp. 29-46). Amsterdam: Elsevier. [More Information]
- Ashish Kumar, T., Manos, J. (2017). Pseudomonas aeruginosa Extracellular Secreted Molecules Have a Dominant Role in Biofilm Development and Bacterial Virulence in Cystic Fibrosis Lung Infections. In Dinesh Sriramulu (Eds.), Progress in Understanding Cystic Fibrosis, (pp. 101-125). Rijeka: InTech Publishers. [More Information]
- Hu, H., Manos, J. (2015). Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa. In Kieran Jordan, Marion Dalmasso (Eds.), Pulse Field Gel Electrophoresis: Methods and Protocols, (pp. 157-170). New York: Springer Science+Business Media. [More Information]
Journals
- Manoharan, A., Farrell, J., Aldilla, V., Whiteley, G., Kriel, E., Glasbey, T., Kumar, N., Moore, K., Manos, J., Ashish Kumar, T. (2023). N-acetylcysteine prevents catheter occlusion and inflammation in catheter associated-urinary tract infections by suppressing urease activity. Frontiers in Cellular and Infection Microbiology, 13. [More Information]
- Ashish Kumar, T., Sabir, S., Chen, R., Farrell, J., Kriel, F., Whiteley, G., Glasbey, T., Manos, J., Willcox, M., Kumar, N. (2022). Halogenated Dihydropyrrol-2-One Molecules Inhibit Pyocyanin Biosynthesis by Blocking the Pseudomonas Quinolone Signaling System. Molecules, 27(4), 1169. [More Information]
- Limantoro, C., Ashish Kumar, T., He, M., Dirin, D., Manos, J., Kovalenko, M., Chrzanowski, W. (2022). Synthesis of Antimicrobial Gallium Nanoparticles Using the Hot Injection Method. ACS Materials Science Au. [More Information]
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2023
- Manoharan, A., Farrell, J., Aldilla, V., Whiteley, G., Kriel, E., Glasbey, T., Kumar, N., Moore, K., Manos, J., Ashish Kumar, T. (2023). N-acetylcysteine prevents catheter occlusion and inflammation in catheter associated-urinary tract infections by suppressing urease activity. Frontiers in Cellular and Infection Microbiology, 13. [More Information]
2022
- Ashish Kumar, T., Sabir, S., Chen, R., Farrell, J., Kriel, F., Whiteley, G., Glasbey, T., Manos, J., Willcox, M., Kumar, N. (2022). Halogenated Dihydropyrrol-2-One Molecules Inhibit Pyocyanin Biosynthesis by Blocking the Pseudomonas Quinolone Signaling System. Molecules, 27(4), 1169. [More Information]
- Limantoro, C., Ashish Kumar, T., He, M., Dirin, D., Manos, J., Kovalenko, M., Chrzanowski, W. (2022). Synthesis of Antimicrobial Gallium Nanoparticles Using the Hot Injection Method. ACS Materials Science Au. [More Information]
- Aiyer, A., Ashish Kumar, T., Whiteley, G., Glasbey, T., Kriel, F., Farrell, J., Manos, J. (2022). The Efficacy of an N-Acetylcysteine–Antibiotic Combination Therapy on Achromobacter xylosoxidans in a Cystic Fibrosis Sputum/Lung Cell Model. Biomedicines, 10(11). [More Information]
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2021
- Manos, J. (2021). Current and emerging therapies to combat cystic fibrosis lung infections. Microorganisms, 9(9), 1874. [More Information]
- Aiyer, A., Manoharan, A., Paino, D., Farrell, J., Whiteley, G., Kriel, F., Glasbey, T., Manos, J., Ashish Kumar, T. (2021). Disruption of biofilms and killing of Burkholderia cenocepacia from cystic fibrosis lung using an antioxidant-antibiotic combination therapy. International Journal of Antimicrobial Agents, 58(2), 106372. [More Information]
- Aiyer, A., Visser, S., Bye, P., Britton, W., Whiteley, G., Glasbey, T., Kriel, F., Farrell, J., Ashish Kumar, T., Manos, J. (2021). Effect of n-acetylcysteine in combination with antibiotics on the biofilms of three cystic fibrosis pathogens of emerging importance. Antibiotics, 10(10), 1176. [More Information]
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2020
- Yang, S., Tran, C., Whiteley, G., Glasbey, T., Kriel, F., McKenzie, D., Manos, J., Ashish Kumar, T. (2020). Covalent immobilization of N-acetylcysteine on a polyvinyl chloride substrate prevents bacterial adhesion and biofilm formation. Langmuir, 36(43), 13023-13033. [More Information]
- Ashish Kumar, T., Manoharan, A., Whiteley, G., Glasbey, T., Manos, J. (2020). Pseudomonas aeruginosa biofilms and infections: Roles of extracellular molecules. In Mukesh Kumar Yadav and Bhim Pratap Singh (Eds.), New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biofilms: Current Research and Future Trends, (pp. 29-46). Amsterdam: Elsevier. [More Information]
- Manoharan, A., Ashish Kumar, T., Whiteley, G., Glasbey, T., Kriel, F., Manos, J. (2020). The effect of N-acetylcysteine in a combined antibiofilm treatment against antibiotic-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy, 75(7), 1787-1798. [More Information]
2019
- Chang, R., Das, T., Manos, J., Kutter, E., Morales, S., Chan, H. (2019). Bacteriophage PEV20 and Ciprofloxacin Combination Treatment Enhances Removal of Pseudomonas aeruginosa Biofilm Isolated from Cystic Fibrosis and Wound Patients. The AAPS Journal, 21(3), 1-8. [More Information]
- Ashish Kumar, T., Paino, D., Manoharan, A., Farrell, J., Whiteley, G., Kriel, F., Glasbey, T., Manos, J. (2019). Conditions Under Which Glutathione Disrupts the Biofilms and Improves Antibiotic Efficacy of Both ESKAPE and Non-ESKAPE Species. Frontiers in Microbiology, 10, 1-16. [More Information]
- Ashish Kumar, T., Choong, H., Kwang, Y., Chan, H., Manos, J., Kwok, P., Duong, H. (2019). Spray-Dried Particles of Nitric Oxide-Modified Glutathione for the Treatment of Chronic Lung Infection. Molecular Pharmaceutics, 16(4), 1723-1731. [More Information]
2017
- Ashish Kumar, T., Simone, M., Ibugo, A., Witting, P., Manefield, M., Manos, J. (2017). Glutathione Enhances Antibiotic Efficiency and Effectiveness of DNase I in Disrupting Pseudomonas aeruginosa Biofilms While Also Inhibiting Pyocyanin Activity, Thus Facilitating Restoration of Cell Enzymatic Activity, Confluence and Viability. Frontiers in Microbiology, 8, 1-18. [More Information]
- Ashish Kumar, T., Manos, J. (2017). Pseudomonas aeruginosa Extracellular Secreted Molecules Have a Dominant Role in Biofilm Development and Bacterial Virulence in Cystic Fibrosis Lung Infections. In Dinesh Sriramulu (Eds.), Progress in Understanding Cystic Fibrosis, (pp. 101-125). Rijeka: InTech Publishers. [More Information]
- Yamaguchi, S., Nath, S., Sugawara, Y., Divakarla, K., Ashish Kumar, T., Manos, J., Chrzanowski, W., Matsushita, T., Kokubo, T. (2017). Two-in-One Biointerfaces-Antimicrobial and Bioactive Nanoporous Gallium Titanate Layers for Titanium Implants. Nanomaterials, 7(8), 1-17. [More Information]
2016
- Klare, W., Ashish Kumar, T., Ibugo, A., Buckle, E., Manefield, M., Manos, J. (2016). Glutathione-Disrupted Biofilms of Clinical Pseudomonas aeruginosa Strains Exhibit an Enhanced Antibiotic Effect and a Novel Biofilm Transcriptome. Antimicrobial Agents and Chemotherapy, 60(8), 4539-4551. [More Information]
2015
- Freschi, L., Jeukens, J., Kukavica-Ibrulj, I., Boyle, B., Dupont, M., Laroche, J., Larose, S., Maaroufi, H., Fothergill, J., Moore, M., Manos, J., et al (2015). Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in Microbiology, 6, 1-8. [More Information]
- Harmer, C., Wynn, M., Pinto, R., Cordwell, S., Rose, B., Harbour, C., Triccas, J., Manos, J. (2015). Homogentisate 1-2-Dioxygenase Downregulation in the Chronic Persistence of Pseudomonas aeruginosa Australian Epidemic Strain-1 in the CF Lung. PloS One, 10(8), 1-13. [More Information]
- Hu, H., Manos, J. (2015). Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa. In Kieran Jordan, Marion Dalmasso (Eds.), Pulse Field Gel Electrophoresis: Methods and Protocols, (pp. 157-170). New York: Springer Science+Business Media. [More Information]
2013
- De Soyza, A., Hall, A., Mahenthiralingam, E., Drevinek, P., Kaca, W., Drulis-Kawa, Z., Stoitsova, S., Toth, V., Coenye, T., Zlosnik, J., Manos, J., et al (2013). Developing an international Pseudomonas aeruginosa reference panel. MicrobiologyOpen, 2(6), 1010-1023. [More Information]
- Harmer, C., Alnassafi, K., Hu, H., Elkins, M., Bye, P., Rose, B., Cordwell, S., Triccas, J., Harbour, C., Manos, J. (2013). Modulation of gene expression by Pseudomonas aeruginosa during chronic infection in the adult cystic fibrosis lung. Microbiology, 159(11), 2354-2363. [More Information]
- Scott, N., Hare, N., White, M., Manos, J., Cordwell, S. (2013). Secretome of Transmissible Pseudomonas aeruginosa AES-1R Grown in a Cystic Fibrosis Lung-Like Environment. Journal of Proteome Research, 12(12), 5357-5369. [More Information]
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2012
- Hare, N., Solis, N., Harmer, C., Marzook, N., Rose, B., Harbour, C., Crossett, B., Manos, J., Cordwell, S. (2012). Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain. BMC Microbiology, 12(January 2012), 1-14. [More Information]
- Hare, N., Soe, C., Rose, B., Harbour, C., Codd, R., Manos, J., Cordwell, S. (2012). Proteomics of Pseudomonas aeruginosa Australian Epidemic Strain 1 (AES-1) Cultured under Conditions Mimicking the Cystic Fibrosis Lung Reveals Increased Iron Acquisition via the Siderophore Pyochelin. Journal of Proteome Research, 11(2), 776-795. [More Information]
- Harmer, C., Triccas, J., Hu, H., Rose, B., Bye, P., Elkins, M., Manos, J. (2012). Pseudomonas aeruginosa strains from the chronically infected cystic fibrosis lung display increased invasiveness of A549 epithelial cells over time. Microbial Pathogenesis, 53(1), 37-43. [More Information]
2011
- Naughton, S., Parker, D., Seemann, T., Thomas, T., Turnbull, L., Rose, B., Bye, P., Cordwell, S., Whitchurch, C., Manos, J. (2011). Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung. PloS One, 6(9), e24526-1-e24526-8. [More Information]
2010
- Tingpej, P., Elkins, M., Rose, B., Hu, H., Moriarty, C., Manos, J., Barras, B., Bye, P., Harbour, C. (2010). Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa. Respirology, 15(6), 923-929. [More Information]
- Fung, C., Naughton, S., Turnbull, L., Tingpej, P., Rose, B., Arthur, J., Hu, H., Harmer, C., Harbour, C., Hassett, D., Manos, J., et al (2010). Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking CF lung sputum. Journal of Medical Microbiology, 59(Pt9), 1089-1100. [More Information]
2009
- Manos, J., Arthur, J., Rose, B., Bell, S., Tingpej, P., Hu, H., Webb, J., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2009). Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth. FEMS Microbiology Letters, 292(1), 107-114. [More Information]
2008
- Manos, J., Arthur, J., Rose, B., Tingpej, P., Fung, C., Curtis, M., Webb, J., Hu, H., Kjelleberg, S., Gorrell, M., Bye, P., Harbour, C. (2008). Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung. Journal of Medical Microbiology, 57(12), 1454-1465. [More Information]
2007
- Tingpej, P., Smith, L., Rose, B., Zhu, H., Conibear, T., Al Nassafi, K., Manos, J., Elkins, M., Bye, P., Willcox, M., Harbour, C., et al (2007). Phenotypic characterization of clonal and nonclonal Pseudomonas aeruginosa strains isolated from lungs of adults with cystic fibrosis. Journal of Clinical Microbiology, 45(6), 1697-1704. [More Information]
2006
- Smith, L., Rose, B., Tingpej, P., Zhu, H., Conibear, T., Manos, J., Bye, P., Elkins, M., Willcox, M., Bell, S., Harbour, C., et al (2006). Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis. Journal of Medical Microbiology, 55(Pt 12), 1641-1644. [More Information]
2004
- Manos, J., Artimovich, E., Belas, R. (2004). Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella. Microbiology, 150(5), 1291-1299. [More Information]
- Belas, R., Manos, J., Suvanasuthi, R. (2004). Proteus mirabilis ZapA Metalloprotease Degrades a Broad Spectrum of Substrates, Including Antimicrobial Peptides. Infection and Immunity, 72(9), 5159-5167. [More Information]
- Manos, J., Belas, R. (2004). Transcription of Proteus mirabilis flaAB. Microbiology, 150(9), 2857-2863. [More Information]
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Selected Grants
2023
- Eradicating Bacterial Vaginosis bacteria, Manos J, Sydney Institute for Infectious Diseases (Sydney ID)/Sydney ID Seed Grants
2021
- Screening nitroxides for antimicrobial activity in 2 models of infection, Witting P, Chami B, Manos J, Oehlers S, Wright K, Centre for Drug Discovery Innovation/DDI and Sydney-ID Joint Seed Funding
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